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The map label for this gene is fhlA [H]

Identifier: 94309493

GI number: 94309493

Start: 583966

End: 585510

Strand: Direct

Name: fhlA [H]

Synonym: Rmet_0548

Alternate gene names: 94309493

Gene position: 583966-585510 (Clockwise)

Preceding gene: 94309490

Following gene: 94309494

Centisome position: 14.87

GC content: 66.99

Gene sequence:

>1545_bases
ATGCACAAGATCGCCGAACCGGGCGGCGCGCTGCTGCTCGACTACGACGATGTCGCACGGCGGGCCATGGAGTCGCTTTT
CCGCACCTTCGAGAATTTTAGCGAGGGGACTTTCGTCGTGGACGACCAGGCGCGCGTGGTGTGGATCAACAAGCGCTACG
CGGCGCGTTTTGGCTTCAGCAATCCGCAGGATGCGATCGGGCTCGATTGCGAGCAGGTGATTCCGCACAGCCTGATGCGC
GAGGTCGTAACAACCGGCAAGCCGATCCTGTTGGACATCCTGGAGACAGGGCGCGAGCCGATGATCGTCACGCGCCTGCC
GATCAAGGACGACGAGGGTCGGACCATCGGCGGTGTGGGCTTCGCATTGTTCGACGAGCTCAAGGCTCTCACGCCGATCT
ATGCCCACTACGCGCGGGTGCAGGCCGAACTGGCTGCCGCGCGGCGATCGCTGGATCAGGCGCGCCGCGCCAAGTACACG
TTCGCGAGCTTCGTTGGCACCAGCCGGGCGGCGCAGGAGGTCAAGCGTCAGGCGCGCCGGGCGGCCCAGGTGGAATCGCC
GGTGCTGCTGCTCGGCGAGACCGGCACCGGCAAGGAACTGCTGGCGCACGGCATTCACGCTGGCTCGGCGCGTGCCGAAA
AGCCGCTCGTGACCGTCAATGTCTCGGCGATTCCCGACACGTTGCTCGAAGTGGAGTTCTTTGGCGCCGCGCCGGGCGCC
TATACCGGTGTCGACCGCAAGGGCCGCGTTGGCAAGTTCGAGCTGGCCGATGGCGGCACGCTGTTTCTCGATGAAATCGG
CGACATGCCGCTCGCGCTGCAGAGCAAGTTGCTGCGCGCGCTCCAGGATCGCGAATTCGAACCGCTTGGCTCCAACCGGA
TCGTGCGATCCGATGTGCGCATCATTGCCGCCACGTCCGCCGACCTGCCGGGGCTGGTGGCGGCAGGCAGGTTCCGCCCG
GACCTCTACTACCGGCTCAACGTGCTGACCATCGAACTGCCGCCGCTGCGGGAGCGACTGGGCGATCTGGTGCCGATGGC
CTACGCAATGATCGAGGAACTCTGCATCAAGGCTGCCCGGCAGCCGATGGGGCTGGGCGCGGACGCATTGAAGCTCCTCG
GCGAGTACGGCTGGCCGGGCAACGTACGCGAACTGCGCAATGTGCTGGAGCGTGTGGTGATGCTGTGCGACGAGGATGCG
ATCGACGCCGCGACACTGGCGCCGCTGCTTGGGCTGGGGCGCGTGCCGTCCGCCATCCATGCGGGCCCTCAATCGGCACC
AGAATCCGCACCCCATCCGACGACAGTGATTCCGGAGACACCCGTGCCGCCGGAGGTGGCAGCAGACGTGGCCATCGCCC
ACGTCCCCGGCGTTCAGACCGCGGTGCCCGCGCAATCCTATGCCGACGCCATGGCGCAGTTCGAAGCGGCCTTCCTGCAT
CAGGCGCTGGACGCCTGCGGAGGCCGTGTAGCCGAGGCCGCCGCCCGCATCGGGCTCAGCCGCGCGGCGTTCTACAAGAA
GCTGGCGGCCGGAAAGGCCAGGTAG

Upstream 100 bases:

>100_bases
AAAATGTCTCCCGATGGAGATGTCGGGTTCTCCCGGGAGACCTTTGAGTCTCTGATATGAGACACTTCGCGTCTCCAAAT
CGAGAATCGAAAACGGGAAC

Downstream 100 bases:

>100_bases
TTCGGGGACGTCACGGCGCCATGCTAACGTCGCGCGTCACACAGTTATTTCGATCCACGGGAGTCTTCATGCAATCGGGC
ATTGTCGAGTCGGGCTACGG

Product: Sigma54 specific transcriptional regulator, Fis-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MHKIAEPGGALLLDYDDVARRAMESLFRTFENFSEGTFVVDDQARVVWINKRYAARFGFSNPQDAIGLDCEQVIPHSLMR
EVVTTGKPILLDILETGREPMIVTRLPIKDDEGRTIGGVGFALFDELKALTPIYAHYARVQAELAAARRSLDQARRAKYT
FASFVGTSRAAQEVKRQARRAAQVESPVLLLGETGTGKELLAHGIHAGSARAEKPLVTVNVSAIPDTLLEVEFFGAAPGA
YTGVDRKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDREFEPLGSNRIVRSDVRIIAATSADLPGLVAAGRFRP
DLYYRLNVLTIELPPLRERLGDLVPMAYAMIEELCIKAARQPMGLGADALKLLGEYGWPGNVRELRNVLERVVMLCDEDA
IDAATLAPLLGLGRVPSAIHAGPQSAPESAPHPTTVIPETPVPPEVAADVAIAHVPGVQTAVPAQSYADAMAQFEAAFLH
QALDACGGRVAEAAARIGLSRAAFYKKLAAGKAR

Sequences:

>Translated_514_residues
MHKIAEPGGALLLDYDDVARRAMESLFRTFENFSEGTFVVDDQARVVWINKRYAARFGFSNPQDAIGLDCEQVIPHSLMR
EVVTTGKPILLDILETGREPMIVTRLPIKDDEGRTIGGVGFALFDELKALTPIYAHYARVQAELAAARRSLDQARRAKYT
FASFVGTSRAAQEVKRQARRAAQVESPVLLLGETGTGKELLAHGIHAGSARAEKPLVTVNVSAIPDTLLEVEFFGAAPGA
YTGVDRKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDREFEPLGSNRIVRSDVRIIAATSADLPGLVAAGRFRP
DLYYRLNVLTIELPPLRERLGDLVPMAYAMIEELCIKAARQPMGLGADALKLLGEYGWPGNVRELRNVLERVVMLCDEDA
IDAATLAPLLGLGRVPSAIHAGPQSAPESAPHPTTVIPETPVPPEVAADVAIAHVPGVQTAVPAQSYADAMAQFEAAFLH
QALDACGGRVAEAAARIGLSRAAFYKKLAAGKAR
>Mature_514_residues
MHKIAEPGGALLLDYDDVARRAMESLFRTFENFSEGTFVVDDQARVVWINKRYAARFGFSNPQDAIGLDCEQVIPHSLMR
EVVTTGKPILLDILETGREPMIVTRLPIKDDEGRTIGGVGFALFDELKALTPIYAHYARVQAELAAARRSLDQARRAKYT
FASFVGTSRAAQEVKRQARRAAQVESPVLLLGETGTGKELLAHGIHAGSARAEKPLVTVNVSAIPDTLLEVEFFGAAPGA
YTGVDRKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDREFEPLGSNRIVRSDVRIIAATSADLPGLVAAGRFRP
DLYYRLNVLTIELPPLRERLGDLVPMAYAMIEELCIKAARQPMGLGADALKLLGEYGWPGNVRELRNVLERVVMLCDEDA
IDAATLAPLLGLGRVPSAIHAGPQSAPESAPHPTTVIPETPVPPEVAADVAIAHVPGVQTAVPAQSYADAMAQFEAAFLH
QALDACGGRVAEAAARIGLSRAAFYKKLAAGKAR

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=263, Percent_Identity=48.2889733840304, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1790437, Length=387, Percent_Identity=39.0180878552972, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082117, Length=263, Percent_Identity=45.2471482889734, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1788550, Length=386, Percent_Identity=35.2331606217617, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1789233, Length=394, Percent_Identity=34.2639593908629, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788905, Length=333, Percent_Identity=39.9399399399399, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786524, Length=372, Percent_Identity=35.752688172043, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=46.3519313304721, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1790299, Length=479, Percent_Identity=31.5240083507307, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787583, Length=298, Percent_Identity=37.5838926174497, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=233, Percent_Identity=42.0600858369099, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=356, Percent_Identity=30.3370786516854, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=35.9307359307359, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55424; Mature: 55424

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIAEPGGALLLDYDDVARRAMESLFRTFENFSEGTFVVDDQARVVWINKRYAARFGFS
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECHHHHHCCCC
NPQDAIGLDCEQVIPHSLMREVVTTGKPILLDILETGREPMIVTRLPIKDDEGRTIGGVG
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEECHHH
FALFDELKALTPIYAHYARVQAELAAARRSLDQARRAKYTFASFVGTSRAAQEVKRQARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AAQVESPVLLLGETGTGKELLAHGIHAGSARAEKPLVTVNVSAIPDTLLEVEFFGAAPGA
HHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHEEEEECCCCCC
YTGVDRKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDREFEPLGSNRIVRSDVR
CCCCCCCCCCCEEEECCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCE
IIAATSADLPGLVAAGRFRPDLYYRLNVLTIELPPLRERLGDLVPMAYAMIEELCIKAAR
EEEECCCCCCCHHHCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHC
QPMGLGADALKLLGEYGWPGNVRELRNVLERVVMLCDEDAIDAATLAPLLGLGRVPSAIH
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH
AGPQSAPESAPHPTTVIPETPVPPEVAADVAIAHVPGVQTAVPAQSYADAMAQFEAAFLH
CCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHH
QALDACGGRVAEAAARIGLSRAAFYKKLAAGKAR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHKIAEPGGALLLDYDDVARRAMESLFRTFENFSEGTFVVDDQARVVWINKRYAARFGFS
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECHHHHHCCCC
NPQDAIGLDCEQVIPHSLMREVVTTGKPILLDILETGREPMIVTRLPIKDDEGRTIGGVG
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCCEECHHH
FALFDELKALTPIYAHYARVQAELAAARRSLDQARRAKYTFASFVGTSRAAQEVKRQARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AAQVESPVLLLGETGTGKELLAHGIHAGSARAEKPLVTVNVSAIPDTLLEVEFFGAAPGA
HHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHEEEEECCCCCC
YTGVDRKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDREFEPLGSNRIVRSDVR
CCCCCCCCCCCEEEECCCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCE
IIAATSADLPGLVAAGRFRPDLYYRLNVLTIELPPLRERLGDLVPMAYAMIEELCIKAAR
EEEECCCCCCCHHHCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHC
QPMGLGADALKLLGEYGWPGNVRELRNVLERVVMLCDEDAIDAATLAPLLGLGRVPSAIH
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHH
AGPQSAPESAPHPTTVIPETPVPPEVAADVAIAHVPGVQTAVPAQSYADAMAQFEAAFLH
CCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHH
QALDACGGRVAEAAARIGLSRAAFYKKLAAGKAR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]