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The map label for this gene is oprM [H]

Identifier: 94309456

GI number: 94309456

Start: 542098

End: 543591

Strand: Reverse

Name: oprM [H]

Synonym: Rmet_0511

Alternate gene names: 94309456

Gene position: 543591-542098 (Counterclockwise)

Preceding gene: 94309457

Following gene: 94309454

Centisome position: 13.84

GC content: 66.27

Gene sequence:

>1494_bases
ATGAAGGCAATTGCCATGTCTCGAAGCCAATTCTCGTTCACCGTCGTCGCGCTGGCTTGTGCGCTGCTGACCGGTTGTGC
GGTCGGCCCTGATTACCAGCGGCCCGATGCGCCCGTGCCCAAGGCCTTCAAGGAAGCCAGCGAGGACATGCCCGCATGGA
CCGGCGACTGGAAAACCGCCGAGCCGCAGGACATCATGGCGCGCCCCGACTGGTGGCGCGTGTTCGACGATCCCGTGCTC
GACGCGCTGATGTCTCAGGTCAAGATCTCGAACCAGAACATCAAGGCGGCGGAAGCACAGTATCGCCAAGCCACGGCATC
GCTGTCTGCGGCACGCGCGGGCTACTTCCCGACGGTCGGCGCCAATGCCGGCGTATCGCGCGGCGCCACGAGCAGCAATG
GCCAGATCATCAACGGCCAGAACCTGACGCTGTCCGCCAACTGGGAAATCGACGTGTGGGGCCGCGTGCGCAGGCAGGTG
GAAAGCGCCCAGGCCGGCGCGCAGGCGAGCGAGGCCGACCTGGCTTCGATCCTGCTCTCCACGCAGGCAACGCTGGCGCA
GAGCTACTTCCTGCTGCGCGTGGCAGACGCCCAGCACGCGCTGCTGGACCGCACCGTGGCCGACTACGCGAAGTCGCTGC
AGCTCGTGCAGAACCAGTACAAGGCGGGCACCGCGCAACGCTCGGACGTGCTGCAGTCGGAAACCCAGCTCAAGTCCGCG
CAAGCACAGCAACTCGATATCCAGATCACGCGGCAGCAGCTTGAACATGCCATCGCGGTACTGGTCGGCAAACCCCCGGC
GGACCTGACCGTGGCGACCGTCGACAACCGCGACGCGATTCCGCGCGTACCGGTGGCCGTGCCGTCGCAGTTGCTGGAAC
GCCGCCCCGATATCTCGGCTGCGGAACGGCGCATGGCATCTGCGAACGCCAATATTGGCGTGGCGGAGGCGGCCTACTAC
CCAACGCTCTCGCTATCGGCCACAGGCGGGCTGACGGCCAGCACCCTGGCGCGCTGGCTCTCCCTGCCCGACCGCGTCTG
GTCAATCGGTGCTGGTCTGGCCGGCACCGTGTTCGACGGCGGCCTGCGCCGTGCCAATACAGCGATTGCCGTGGCCCAAT
ACGACCAGACCGTGGCGAACTATCGGCAGACCGTGCTGGGCGCGTTCCAGGAGGTCGAGGACAACCTCGCCTCGGCCCGC
GTGCTCGAACAGGAGTCGATCGTCCAGAACGACGCGCTGCGCTCGGCACGTGAAGCGCTTTCGCTGGTCAACAACCGCTA
CAAGGCCGGCACCGCAGCGTTGCTGGATGTGCTGACGGCACAGACCGCGGCCTATACGGCGGAGAACACCACATTGACGA
TCATGGGGCGGCAGTACACAGCGGCCATCACGCTGATCAAGGCGCTTGGCGGCGGATGGCATGGGCAGGCTATGGAGGTG
GGGAAGGTCGATAGCACCGCGCCAGCTACCGCGCCTGCATCGACGGCGCAGTAA

Upstream 100 bases:

>100_bases
TGGTCTACCTCACGCTGGATCGCTGGCGCCTCAAGGTCAAGGCCTGGCGCGAGCGTCGTCGCACCCAGGCAAGCGGCACC
ACGCCGCACCTCGAACACTG

Downstream 100 bases:

>100_bases
CAGACTCGGTGGCAGACTCAATAGCGGCGCTGCAGCGCGCTACCGCCCCCCAGGCATCGGCAACGGCTGCTGACTGACCC
GCTCCCGCAGCCATGTGGCC

Product: cation/multidrug efflux system outer membrane porin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKAIAMSRSQFSFTVVALACALLTGCAVGPDYQRPDAPVPKAFKEASEDMPAWTGDWKTAEPQDIMARPDWWRVFDDPVL
DALMSQVKISNQNIKAAEAQYRQATASLSAARAGYFPTVGANAGVSRGATSSNGQIINGQNLTLSANWEIDVWGRVRRQV
ESAQAGAQASEADLASILLSTQATLAQSYFLLRVADAQHALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
QAQQLDIQITRQQLEHAIAVLVGKPPADLTVATVDNRDAIPRVPVAVPSQLLERRPDISAAERRMASANANIGVAEAAYY
PTLSLSATGGLTASTLARWLSLPDRVWSIGAGLAGTVFDGGLRRANTAIAVAQYDQTVANYRQTVLGAFQEVEDNLASAR
VLEQESIVQNDALRSAREALSLVNNRYKAGTAALLDVLTAQTAAYTAENTTLTIMGRQYTAAITLIKALGGGWHGQAMEV
GKVDSTAPATAPASTAQ

Sequences:

>Translated_497_residues
MKAIAMSRSQFSFTVVALACALLTGCAVGPDYQRPDAPVPKAFKEASEDMPAWTGDWKTAEPQDIMARPDWWRVFDDPVL
DALMSQVKISNQNIKAAEAQYRQATASLSAARAGYFPTVGANAGVSRGATSSNGQIINGQNLTLSANWEIDVWGRVRRQV
ESAQAGAQASEADLASILLSTQATLAQSYFLLRVADAQHALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
QAQQLDIQITRQQLEHAIAVLVGKPPADLTVATVDNRDAIPRVPVAVPSQLLERRPDISAAERRMASANANIGVAEAAYY
PTLSLSATGGLTASTLARWLSLPDRVWSIGAGLAGTVFDGGLRRANTAIAVAQYDQTVANYRQTVLGAFQEVEDNLASAR
VLEQESIVQNDALRSAREALSLVNNRYKAGTAALLDVLTAQTAAYTAENTTLTIMGRQYTAAITLIKALGGGWHGQAMEV
GKVDSTAPATAPASTAQ
>Mature_497_residues
MKAIAMSRSQFSFTVVALACALLTGCAVGPDYQRPDAPVPKAFKEASEDMPAWTGDWKTAEPQDIMARPDWWRVFDDPVL
DALMSQVKISNQNIKAAEAQYRQATASLSAARAGYFPTVGANAGVSRGATSSNGQIINGQNLTLSANWEIDVWGRVRRQV
ESAQAGAQASEADLASILLSTQATLAQSYFLLRVADAQHALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
QAQQLDIQITRQQLEHAIAVLVGKPPADLTVATVDNRDAIPRVPVAVPSQLLERRPDISAAERRMASANANIGVAEAAYY
PTLSLSATGGLTASTLARWLSLPDRVWSIGAGLAGTVFDGGLRRANTAIAVAQYDQTVANYRQTVLGAFQEVEDNLASAR
VLEQESIVQNDALRSAREALSLVNNRYKAGTAALLDVLTAQTAAYTAENTTLTIMGRQYTAAITLIKALGGGWHGQAMEV
GKVDSTAPATAPASTAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=468, Percent_Identity=28.4188034188034, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI2367348, Length=484, Percent_Identity=24.5867768595041, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52975; Mature: 52975

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIAMSRSQFSFTVVALACALLTGCAVGPDYQRPDAPVPKAFKEASEDMPAWTGDWKTA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
EPQDIMARPDWWRVFDDPVLDALMSQVKISNQNIKAAEAQYRQATASLSAARAGYFPTVG
CCHHHCCCCCHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
ANAGVSRGATSSNGQIINGQNLTLSANWEIDVWGRVRRQVESAQAGAQASEADLASILLS
CCCCCCCCCCCCCCEEECCCEEEEEECCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TQATLAQSYFLLRVADAQHALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
HHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QAQQLDIQITRQQLEHAIAVLVGKPPADLTVATVDNRDAIPRVPVAVPSQLLERRPDISA
HHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCH
AERRMASANANIGVAEAAYYPTLSLSATGGLTASTLARWLSLPDRVWSIGAGLAGTVFDG
HHHHHHHCCCCCCEEHHHHCCCEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHCC
GLRRANTAIAVAQYDQTVANYRQTVLGAFQEVEDNLASARVLEQESIVQNDALRSAREAL
CHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVNNRYKAGTAALLDVLTAQTAAYTAENTTLTIMGRQYTAAITLIKALGGGWHGQAMEV
HHHHCCCCHHHHHHHHHHHHHHHHEEECCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEC
GKVDSTAPATAPASTAQ
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKAIAMSRSQFSFTVVALACALLTGCAVGPDYQRPDAPVPKAFKEASEDMPAWTGDWKTA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
EPQDIMARPDWWRVFDDPVLDALMSQVKISNQNIKAAEAQYRQATASLSAARAGYFPTVG
CCHHHCCCCCHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
ANAGVSRGATSSNGQIINGQNLTLSANWEIDVWGRVRRQVESAQAGAQASEADLASILLS
CCCCCCCCCCCCCCEEECCCEEEEEECCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TQATLAQSYFLLRVADAQHALLDRTVADYAKSLQLVQNQYKAGTAQRSDVLQSETQLKSA
HHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QAQQLDIQITRQQLEHAIAVLVGKPPADLTVATVDNRDAIPRVPVAVPSQLLERRPDISA
HHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCH
AERRMASANANIGVAEAAYYPTLSLSATGGLTASTLARWLSLPDRVWSIGAGLAGTVFDG
HHHHHHHCCCCCCEEHHHHCCCEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHCC
GLRRANTAIAVAQYDQTVANYRQTVLGAFQEVEDNLASARVLEQESIVQNDALRSAREAL
CHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVNNRYKAGTAALLDVLTAQTAAYTAENTTLTIMGRQYTAAITLIKALGGGWHGQAMEV
HHHHCCCCHHHHHHHHHHHHHHHHEEECCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEC
GKVDSTAPATAPASTAQ
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]