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The map label for this gene is dacB [H]

Identifier: 94309453

GI number: 94309453

Start: 538842

End: 540398

Strand: Reverse

Name: dacB [H]

Synonym: Rmet_0508

Alternate gene names: 94309453

Gene position: 540398-538842 (Counterclockwise)

Preceding gene: 335055496

Following gene: 94309445

Centisome position: 13.76

GC content: 66.54

Gene sequence:

>1557_bases
ATGAGCGCCATGCCAATCCGCCCCTCCCTGCTCCGCCGCAGCCTGTCTCCCGTTATCGCAACGGCCGCGCTGCTCGCCTT
CGGCCTGACTGGAGCCCAGGCCAAACCCGTCAAATCCGTCAAAGCCACCAAACCGTCGGTCCAGCGCGTCAGTGCAGAAG
CCGCGGACCCGGCCGCCGCGCGCACCGGCCTGCCCGCTACTGTCACCACGGCGCTGCGCCGCGCGCATGTGCCGCTGTCG
GCAGCAAGCTTCTACGTGATCAAGGTCGGCGCGCCGCGCCCGCGTGTGAGCTGGAACGCCGAGGCGCCGATGAACCCGGC
GTCGACGATGAAGCTGGTCACCACGTTCGCCGGCCTGCAATTGCTCGGGCCCGACTATCGCTGGCTCACTTCGCTCTACG
CCGATGGCCAGCCGGGTCCGGACGGGGTGATCAACGGCAACGTCTATCTGCGCGGCCGGGGAGACCCGAAGCTGGTGCCC
GAGGAGATGGCCAAGCTGGTCAACACGGCGCGATCGACAGGCGCAACGGCCATCAACGGCGATCTCGTGCTCGACCGCAG
CTTCTTCGCGGATACCCAGGACAACAGCTACACGATCGATGGCGAGACGCAACGCGCCTACAACGTCAATCCTGATGCGC
TGCTTTATGCCTTCAAGACGCTCACCTTCACGATCACGCCCGACGCCGCCGGCCGCGCCGTCGACGTGGCCGTCACGCCG
GCGCTGGCGCAATTGCGTGTGGATAACCGCCTGACGCTGGTCAACGGCCGTTGCGGTGACTGGCGCGCACGTGCCAACCC
AAGCATCACGCCGCAGTCAGACGGCACCATCGTCGCCGCGTTCGACGGCAACTACTCCGCCGATTGTGGCGAACACGTGG
TCAACCTGGCCACGCTCTCGCACAGCGATTTCATCTGGGGCGGCTTCGTGGCCGAATGGCAGGCGGCGGGTGGCCGCTTC
GTGCGTCCGCCCGGGCTGCGTGGCGGCACCGTGCCGCGCGGCGCGTTCCTGCTGGCGCGGCACTACGGCCAGCCGCTCGG
CGAGATCGTGCGCGACATCAACAAGTTCTCGAACAACGTGATGGCACGTCAGCTCTTCCTGACGATTGGCGCGGAGATCG
ACCGGAGCGGCCCAGCCAGCACCGAGAAGTCGACGCGCGTGATCAAGCGCTGGCTATCGCGCCAGGGACTGGACATGCCG
GATCTGGTACTGGAAAACGGCTCGGGACTGTCACGCGAGGAACGGATCAGCGCTTATGACATGGCGCGTCTGCTGCAACA
GGCACTGGCCAGCAATGTTGGGCCGGTGCTGATGGACTCCCTGCCGATCCTCGGCGTGGATGGCACCCTGCGCAATCGCC
TGACCCGGGCCAATGCGGCCGGCAATGCCTACCTGAAGACGGGCACACTGCAGGACGTACGCGCGCTGGCGGGCTACGTC
GACGCGCTCGACGGGCAGCGTTATGTGGTGGTGGCGTATATCAATCACCCGAACGCATCGGGAGCGCAGGAGGCGCACGA
TGCGTTGATGCAGTGGGTGTATAAGGGCACGCCTTGA

Upstream 100 bases:

>100_bases
ACCGTGCGACGCGCGGTCGACCTCAGCAACAAAATGCGGCCGGAAACGGTCGAAAACGGCCGCATTCACGTCATCCATCG
GGCATGGGCCATGCGTTAAC

Downstream 100 bases:

>100_bases
GGTGAGGGTCTTGTGACAGGCGTGGTGGGGGATGGAGCGCCTGGCCTTCCCCTCGCCTTCCCCCAACCTCAGCAATCCTC
GAACGCAGCCGCGGCGCGAC

Product: D-Alanyl-D-alanine carboxypeptidase, S13 family

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSAMPIRPSLLRRSLSPVIATAALLAFGLTGAQAKPVKSVKATKPSVQRVSAEAADPAAARTGLPATVTTALRRAHVPLS
AASFYVIKVGAPRPRVSWNAEAPMNPASTMKLVTTFAGLQLLGPDYRWLTSLYADGQPGPDGVINGNVYLRGRGDPKLVP
EEMAKLVNTARSTGATAINGDLVLDRSFFADTQDNSYTIDGETQRAYNVNPDALLYAFKTLTFTITPDAAGRAVDVAVTP
ALAQLRVDNRLTLVNGRCGDWRARANPSITPQSDGTIVAAFDGNYSADCGEHVVNLATLSHSDFIWGGFVAEWQAAGGRF
VRPPGLRGGTVPRGAFLLARHYGQPLGEIVRDINKFSNNVMARQLFLTIGAEIDRSGPASTEKSTRVIKRWLSRQGLDMP
DLVLENGSGLSREERISAYDMARLLQQALASNVGPVLMDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYV
DALDGQRYVVVAYINHPNASGAQEAHDALMQWVYKGTP

Sequences:

>Translated_518_residues
MSAMPIRPSLLRRSLSPVIATAALLAFGLTGAQAKPVKSVKATKPSVQRVSAEAADPAAARTGLPATVTTALRRAHVPLS
AASFYVIKVGAPRPRVSWNAEAPMNPASTMKLVTTFAGLQLLGPDYRWLTSLYADGQPGPDGVINGNVYLRGRGDPKLVP
EEMAKLVNTARSTGATAINGDLVLDRSFFADTQDNSYTIDGETQRAYNVNPDALLYAFKTLTFTITPDAAGRAVDVAVTP
ALAQLRVDNRLTLVNGRCGDWRARANPSITPQSDGTIVAAFDGNYSADCGEHVVNLATLSHSDFIWGGFVAEWQAAGGRF
VRPPGLRGGTVPRGAFLLARHYGQPLGEIVRDINKFSNNVMARQLFLTIGAEIDRSGPASTEKSTRVIKRWLSRQGLDMP
DLVLENGSGLSREERISAYDMARLLQQALASNVGPVLMDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYV
DALDGQRYVVVAYINHPNASGAQEAHDALMQWVYKGTP
>Mature_517_residues
SAMPIRPSLLRRSLSPVIATAALLAFGLTGAQAKPVKSVKATKPSVQRVSAEAADPAAARTGLPATVTTALRRAHVPLSA
ASFYVIKVGAPRPRVSWNAEAPMNPASTMKLVTTFAGLQLLGPDYRWLTSLYADGQPGPDGVINGNVYLRGRGDPKLVPE
EMAKLVNTARSTGATAINGDLVLDRSFFADTQDNSYTIDGETQRAYNVNPDALLYAFKTLTFTITPDAAGRAVDVAVTPA
LAQLRVDNRLTLVNGRCGDWRARANPSITPQSDGTIVAAFDGNYSADCGEHVVNLATLSHSDFIWGGFVAEWQAAGGRFV
RPPGLRGGTVPRGAFLLARHYGQPLGEIVRDINKFSNNVMARQLFLTIGAEIDRSGPASTEKSTRVIKRWLSRQGLDMPD
LVLENGSGLSREERISAYDMARLLQQALASNVGPVLMDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYVD
ALDGQRYVVVAYINHPNASGAQEAHDALMQWVYKGTP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=462, Percent_Identity=25.7575757575758, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 55435; Mature: 55303

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMPIRPSLLRRSLSPVIATAALLAFGLTGAQAKPVKSVKATKPSVQRVSAEAADPAAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCHHH
RTGLPATVTTALRRAHVPLSAASFYVIKVGAPRPRVSWNAEAPMNPASTMKLVTTFAGLQ
HCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHH
LLGPDYRWLTSLYADGQPGPDGVINGNVYLRGRGDPKLVPEEMAKLVNTARSTGATAING
EECCCHHHHHHHHCCCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECC
DLVLDRSFFADTQDNSYTIDGETQRAYNVNPDALLYAFKTLTFTITPDAAGRAVDVAVTP
CEEEECHHCCCCCCCCEEECCCCCCEECCCHHHHHEEEEEEEEEECCCCCCCEEEEEECC
ALAQLRVDNRLTLVNGRCGDWRARANPSITPQSDGTIVAAFDGNYSADCGEHVVNLATLS
HHHHEEECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHEEC
HSDFIWGGFVAEWQAAGGRFVRPPGLRGGTVPRGAFLLARHYGQPLGEIVRDINKFSNNV
CCCCEECCHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
MARQLFLTIGAEIDRSGPASTEKSTRVIKRWLSRQGLDMPDLVLENGSGLSREERISAYD
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHEECCCCCCHHHHHHHHH
MARLLQQALASNVGPVLMDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYV
HHHHHHHHHHCCCCHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHH
DALDGQRYVVVAYINHPNASGAQEAHDALMQWVYKGTP
HHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAMPIRPSLLRRSLSPVIATAALLAFGLTGAQAKPVKSVKATKPSVQRVSAEAADPAAA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCHHH
RTGLPATVTTALRRAHVPLSAASFYVIKVGAPRPRVSWNAEAPMNPASTMKLVTTFAGLQ
HCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHH
LLGPDYRWLTSLYADGQPGPDGVINGNVYLRGRGDPKLVPEEMAKLVNTARSTGATAING
EECCCHHHHHHHHCCCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECC
DLVLDRSFFADTQDNSYTIDGETQRAYNVNPDALLYAFKTLTFTITPDAAGRAVDVAVTP
CEEEECHHCCCCCCCCEEECCCCCCEECCCHHHHHEEEEEEEEEECCCCCCCEEEEEECC
ALAQLRVDNRLTLVNGRCGDWRARANPSITPQSDGTIVAAFDGNYSADCGEHVVNLATLS
HHHHEEECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHEEC
HSDFIWGGFVAEWQAAGGRFVRPPGLRGGTVPRGAFLLARHYGQPLGEIVRDINKFSNNV
CCCCEECCHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
MARQLFLTIGAEIDRSGPASTEKSTRVIKRWLSRQGLDMPDLVLENGSGLSREERISAYD
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHEECCCCCCHHHHHHHHH
MARLLQQALASNVGPVLMDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYV
HHHHHHHHHHCCCCHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHH
DALDGQRYVVVAYINHPNASGAQEAHDALMQWVYKGTP
HHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1554361; 7305936 [H]