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The map label for this gene is ilvE [H]

Identifier: 94309438

GI number: 94309438

Start: 519972

End: 520892

Strand: Reverse

Name: ilvE [H]

Synonym: Rmet_0493

Alternate gene names: 94309438

Gene position: 520892-519972 (Counterclockwise)

Preceding gene: 94309444

Following gene: 94309437

Centisome position: 13.26

GC content: 62.65

Gene sequence:

>921_bases
ATGTCGATGGCAGATCGCGACGGCAAGATCTGGATGGATGGCAAGCTGATTGAGTGGCGTGACGCAAAGATTCACGTACT
CACGCACACGCTGCATTACGGCATGGGTGTATTTGAAGGCGTGCGCGCCTACAAGACGCCGGAAGGCACCGCGATTTTCC
GCCTCAAGGAGCACACGCGCCGCCTGTTCAACTCTGCCAAGATCTTCCAGATGGCCATGCCGTTCGACGAGGCCACGCTC
GAAGCCGCCCAGCGCGAAGTTGTGCGCGCCAACAACCTTGAGTCGTGCTATATCCGTCCGCTGGTGTGGATCGGCTCCGA
AAAGCTCGGTGTGTCGGCCAAGGGCAACACGATTCACGTGGCGATCGCCGCCTGGCCCTGGGGCGCGTATCTGGGCGAGG
AAGGCCTGGAACGTGGCATCCGCGTGAAGACCTCGTCGTTCACGCGCCATCACGTGAATGTGTCGCTGGTGCGCGCCAAG
GCATCGGGCTACTACATCAACTCGATCCTGGCCAACCAGGAAGCCACCGGCCTGGGCTACGACGAAGCCCTGCTGCTGGA
TACCGAAGGCTACGTGAGCGAAGGCTCTGGCGAGAATGTGTTCATCGTGCGCAACGGCGTGATCTACACGCCGGACCTGG
CCTCGTGCCTGGACGGCATCACGCGCGACGCCACGCTGACAATCGCCCGTGACCTCGGCATCGAGGTGCGCGAGAAACGC
ATCACGCGTGACGAGATGTACTGCGCTGACGAAGCGTTCTTCACCGGCACCGCCGCCGAAGTGACGCCGATCCGCGAGCT
GGACGACCGTGTCATCGGCGAAGGCCGCCGCGGCCCCGTTACCAAGCGCATCCAGGACGCGTTCTTCGCGGCCGTGGGTG
GCACCGACGAAAAGTACAAGAAGTGGCTGACGCTGGTCTGA

Upstream 100 bases:

>100_bases
TGTAGAACGCTCTATCCGCCGCCCTTCGTTGGCACGCAAGGACGCGCGGCTTACAATGGTGCCTTTGCGACATTTACCTG
GGCTGCAGAGGGAGACGACC

Downstream 100 bases:

>100_bases
TCGACGGCGCCGACCCTGCATTTCCAATCGACATCCCTGCAAGACAGAAAAGCAACACCATGACTCAAACCGCATCCGTC
GTAGAAATCGGCGCAGAAGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTPEGTAIFRLKEHTRRLFNSAKIFQMAMPFDEATL
EAAQREVVRANNLESCYIRPLVWIGSEKLGVSAKGNTIHVAIAAWPWGAYLGEEGLERGIRVKTSSFTRHHVNVSLVRAK
ASGYYINSILANQEATGLGYDEALLLDTEGYVSEGSGENVFIVRNGVIYTPDLASCLDGITRDATLTIARDLGIEVREKR
ITRDEMYCADEAFFTGTAAEVTPIRELDDRVIGEGRRGPVTKRIQDAFFAAVGGTDEKYKKWLTLV

Sequences:

>Translated_306_residues
MSMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTPEGTAIFRLKEHTRRLFNSAKIFQMAMPFDEATL
EAAQREVVRANNLESCYIRPLVWIGSEKLGVSAKGNTIHVAIAAWPWGAYLGEEGLERGIRVKTSSFTRHHVNVSLVRAK
ASGYYINSILANQEATGLGYDEALLLDTEGYVSEGSGENVFIVRNGVIYTPDLASCLDGITRDATLTIARDLGIEVREKR
ITRDEMYCADEAFFTGTAAEVTPIRELDDRVIGEGRRGPVTKRIQDAFFAAVGGTDEKYKKWLTLV
>Mature_305_residues
SMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTPEGTAIFRLKEHTRRLFNSAKIFQMAMPFDEATLE
AAQREVVRANNLESCYIRPLVWIGSEKLGVSAKGNTIHVAIAAWPWGAYLGEEGLERGIRVKTSSFTRHHVNVSLVRAKA
SGYYINSILANQEATGLGYDEALLLDTEGYVSEGSGENVFIVRNGVIYTPDLASCLDGITRDATLTIARDLGIEVREKRI
TRDEMYCADEAFFTGTAAEVTPIRELDDRVIGEGRRGPVTKRIQDAFFAAVGGTDEKYKKWLTLV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=291, Percent_Identity=26.8041237113402, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=49.4983277591973, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17565728, Length=281, Percent_Identity=25.6227758007117, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322002, Length=265, Percent_Identity=28.6792452830189, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34171; Mature: 34040

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTPEGTAIFRLKEHTR
CCCCCCCCEEEECCEEEEEECCEEEEEEEHHHHCCHHHHHHHHHCCCCCCEEEEEHHHHH
RLFNSAKIFQMAMPFDEATLEAAQREVVRANNLESCYIRPLVWIGSEKLGVSAKGNTIHV
HHHCCHHEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEECCCEEEE
AIAAWPWGAYLGEEGLERGIRVKTSSFTRHHVNVSLVRAKASGYYINSILANQEATGLGY
EEEECCCHHHHCHHHHHCCCEEEECCCEEEEEEEEEEEEECCCEEEEHHHCCCCCCCCCC
DEALLLDTEGYVSEGSGENVFIVRNGVIYTPDLASCLDGITRDATLTIARDLGIEVREKR
CCEEEEECCCCEECCCCCEEEEEECCEEECCCHHHHHCCCCCCEEEEEEECCCCHHHHHH
ITRDEMYCADEAFFTGTAAEVTPIRELDDRVIGEGRRGPVTKRIQDAFFAAVGGTDEKYK
CCHHHHHCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCHHHHH
KWLTLV
HHHCCC
>Mature Secondary Structure 
SMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTPEGTAIFRLKEHTR
CCCCCCCEEEECCEEEEEECCEEEEEEEHHHHCCHHHHHHHHHCCCCCCEEEEEHHHHH
RLFNSAKIFQMAMPFDEATLEAAQREVVRANNLESCYIRPLVWIGSEKLGVSAKGNTIHV
HHHCCHHEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEECCCEEEE
AIAAWPWGAYLGEEGLERGIRVKTSSFTRHHVNVSLVRAKASGYYINSILANQEATGLGY
EEEECCCHHHHCHHHHHCCCEEEECCCEEEEEEEEEEEEECCCEEEEHHHCCCCCCCCCC
DEALLLDTEGYVSEGSGENVFIVRNGVIYTPDLASCLDGITRDATLTIARDLGIEVREKR
CCEEEEECCCCEECCCCCEEEEEECCEEECCCHHHHHCCCCCCEEEEEEECCCCHHHHHH
ITRDEMYCADEAFFTGTAAEVTPIRELDDRVIGEGRRGPVTKRIQDAFFAAVGGTDEKYK
CCHHHHHCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCHHHHH
KWLTLV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]