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The map label for this gene is rfaF [H]

Identifier: 94309436

GI number: 94309436

Start: 518580

End: 519644

Strand: Reverse

Name: rfaF [H]

Synonym: Rmet_0491

Alternate gene names: 94309436

Gene position: 519644-518580 (Counterclockwise)

Preceding gene: 94309437

Following gene: 94309435

Centisome position: 13.23

GC content: 66.48

Gene sequence:

>1065_bases
ATGCCCGGGCACCGCATCACAGGGCCACGCAATACCCACAATATGAGAAAAGCCCTCGTCATTGCCCCGAACTGGATCGG
CGACGCGCTGATGGCGCAGCCGCTGTTCGCGCTGCTCAAGGCCCGTCACCCGCAACTGGTGATCGACGCGCTCGCCCCCA
AATGGGTGGCGCCCGTGCTGGCGCGCATGCCGGAGATCGGCAAGGTCTTCCCGACCGATCTGGCACACGGCAAGCTGCAA
TTGAGCGCGCGCCTGCTGTTCGCGCAACAGCTCAAGCACGAAGGCTACGACATGGCCTACGTGCTGCCGAATTCGCTGAA
ATCCGCGCTGATTCCGTTTCTGGCCGGCATTCCACTGCGCATCGGCTATCGTGGCGAAGCACGCTTCGGCATGCTCAACG
TGCGCCACGCCAATCCGCCGCGTGACAAGCGGCCGCCGATGGTGGAGCACTACGCGCGTCTGGCGCTCAAGCCCGGCGCC
CGCCTGCCCGAGTCGATTCCCGACCCGAAGCTCAAGAGCGATCCCAGGCGCATGGCCGCCACGGCCGAGCGCTTCGGCAT
CGCGCCGGGCACACGTCTGATCTCGTTCTGCCCGGGTGCGGAGTTCGGCCCGGCCAAGCGCTGGCCGGCCGCGCACTTCG
CCGCGCTGGCGCAGATGCTGCGTCGCTCGTTCCCGTACGCGCAGATCATCGCGCTCGGCTCGGCCAAAGATGCCGAGATC
GCCGACGAGATCGTCAAAGGCGCGCCGTTCGTCAGGAATCTGTGCGGTCAGACATCGCTCGACGATGCCGTGGACCTGCT
GGCGCTCTCAGAGGCCGCGGTCTGCAACGACTCGGGCCTGATGCACGTCACCGCCGCCCTGAACCGGCCGCAGGTAGCCG
TTTTCGGCTCCAGCGATCCGCGCCACACGCCCCCGCTGTCACAGGCAGCGAGTATCATGTGGCTTCAACTCGAGTGCAGT
CCCTGCTTCAAGCGCGAGTGCCCGCTCGGCCACCTGCGCTGCCTGAAGGAGATCGAGCCCGAAATGGTATTTGTCGAGCT
GCGCAAGCTCCTGCACCGCCCCTGA

Upstream 100 bases:

>100_bases
GGCACCGTACTGAAGGGTCATCACTGACCCGCCCGGCCCTCGCCAATTCCTGTCGTTTCGACCTGACGCCCCGGGCCGCC
CAGCGCCCCGGGGCGTTGAC

Downstream 100 bases:

>100_bases
CTCATCCAACATGCCTCGTTTCGCCCGCCTGTTCGATTCCGCCGAAGACATCGTCGATGCCTTCCGTGAAGCCATGAAGT
TGCGCGACGCCGATGGCGCG

Product: ADP-heptose:LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPGHRITGPRNTHNMRKALVIAPNWIGDALMAQPLFALLKARHPQLVIDALAPKWVAPVLARMPEIGKVFPTDLAHGKLQ
LSARLLFAQQLKHEGYDMAYVLPNSLKSALIPFLAGIPLRIGYRGEARFGMLNVRHANPPRDKRPPMVEHYARLALKPGA
RLPESIPDPKLKSDPRRMAATAERFGIAPGTRLISFCPGAEFGPAKRWPAAHFAALAQMLRRSFPYAQIIALGSAKDAEI
ADEIVKGAPFVRNLCGQTSLDDAVDLLALSEAAVCNDSGLMHVTAALNRPQVAVFGSSDPRHTPPLSQAASIMWLQLECS
PCFKRECPLGHLRCLKEIEPEMVFVELRKLLHRP

Sequences:

>Translated_354_residues
MPGHRITGPRNTHNMRKALVIAPNWIGDALMAQPLFALLKARHPQLVIDALAPKWVAPVLARMPEIGKVFPTDLAHGKLQ
LSARLLFAQQLKHEGYDMAYVLPNSLKSALIPFLAGIPLRIGYRGEARFGMLNVRHANPPRDKRPPMVEHYARLALKPGA
RLPESIPDPKLKSDPRRMAATAERFGIAPGTRLISFCPGAEFGPAKRWPAAHFAALAQMLRRSFPYAQIIALGSAKDAEI
ADEIVKGAPFVRNLCGQTSLDDAVDLLALSEAAVCNDSGLMHVTAALNRPQVAVFGSSDPRHTPPLSQAASIMWLQLECS
PCFKRECPLGHLRCLKEIEPEMVFVELRKLLHRP
>Mature_353_residues
PGHRITGPRNTHNMRKALVIAPNWIGDALMAQPLFALLKARHPQLVIDALAPKWVAPVLARMPEIGKVFPTDLAHGKLQL
SARLLFAQQLKHEGYDMAYVLPNSLKSALIPFLAGIPLRIGYRGEARFGMLNVRHANPPRDKRPPMVEHYARLALKPGAR
LPESIPDPKLKSDPRRMAATAERFGIAPGTRLISFCPGAEFGPAKRWPAAHFAALAQMLRRSFPYAQIIALGSAKDAEIA
DEIVKGAPFVRNLCGQTSLDDAVDLLALSEAAVCNDSGLMHVTAALNRPQVAVFGSSDPRHTPPLSQAASIMWLQLECSP
CFKRECPLGHLRCLKEIEPEMVFVELRKLLHRP

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=347, Percent_Identity=44.956772334294, Blast_Score=282, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38975; Mature: 38844

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGHRITGPRNTHNMRKALVIAPNWIGDALMAQPLFALLKARHPQLVIDALAPKWVAPVL
CCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCHHHHHHHH
ARMPEIGKVFPTDLAHGKLQLSARLLFAQQLKHEGYDMAYVLPNSLKSALIPFLAGIPLR
HHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCEE
IGYRGEARFGMLNVRHANPPRDKRPPMVEHYARLALKPGARLPESIPDPKLKSDPRRMAA
EECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
TAERFGIAPGTRLISFCPGAEFGPAKRWPAAHFAALAQMLRRSFPYAQIIALGSAKDAEI
HHHHHCCCCCCHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCCCHHH
ADEIVKGAPFVRNLCGQTSLDDAVDLLALSEAAVCNDSGLMHVTAALNRPQVAVFGSSDP
HHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCC
RHTPPLSQAASIMWLQLECSPCFKRECPLGHLRCLKEIEPEMVFVELRKLLHRP
CCCCCHHHHCEEEEEEEECCHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PGHRITGPRNTHNMRKALVIAPNWIGDALMAQPLFALLKARHPQLVIDALAPKWVAPVL
CCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCHHHHHHHH
ARMPEIGKVFPTDLAHGKLQLSARLLFAQQLKHEGYDMAYVLPNSLKSALIPFLAGIPLR
HHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCEE
IGYRGEARFGMLNVRHANPPRDKRPPMVEHYARLALKPGARLPESIPDPKLKSDPRRMAA
EECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
TAERFGIAPGTRLISFCPGAEFGPAKRWPAAHFAALAQMLRRSFPYAQIIALGSAKDAEI
HHHHHCCCCCCHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEECCCCCHHH
ADEIVKGAPFVRNLCGQTSLDDAVDLLALSEAAVCNDSGLMHVTAALNRPQVAVFGSSDP
HHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCC
RHTPPLSQAASIMWLQLECSPCFKRECPLGHLRCLKEIEPEMVFVELRKLLHRP
CCCCCHHHHCEEEEEEEECCHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]