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The map label for this gene is gloA [H]

Identifier: 94309387

GI number: 94309387

Start: 468718

End: 469125

Strand: Reverse

Name: gloA [H]

Synonym: Rmet_0442

Alternate gene names: 94309387

Gene position: 469125-468718 (Counterclockwise)

Preceding gene: 94309388

Following gene: 94309386

Centisome position: 11.94

GC content: 63.48

Gene sequence:

>408_bases
ATGCGACTCCTCCACACCATGCTGCGCGTTGGCGACTACCAGCGTTCGATCGACTTCTACACGCGCGTGCTGGGCATGAC
GCTGCTGCGCGAAAGCGACAATCCCGAATACAAGTACCGCCTGGCCTTCGTCGGATACGGCCCCGAAACCGAGACCGCCG
TCATCGAACTGACCTACAACTACGGCGTCGACAAGTACGATCTTGGTACCGCCTATGGCCATATCGCCCTGGAAGTACCC
AGCGCGGCCGAAGCCTGTGAGCGCATCCGGTCGGCCGGCGGCAAGGTCACCCGCGAAGCCGGTCCGGTCAAGGGCGGCAC
CACGGTGATCGCGTTCGTAGAGGACCCGGACGGCTACAAGATCGAACTGATCGAGCGTCACTCGACGCGCGACGCTGCCC
GCCCGTAA

Upstream 100 bases:

>100_bases
ATATCGGCATAGGGACCGACATTGACGGCCGCCGACGCGTTCCGCGGCATGGGAATAGAATGGCGAACTCTGTCTTTACA
GTTTTGCAAAAGAGACCCCC

Downstream 100 bases:

>100_bases
CGCGCAATGAGCGCGCAAGCAATGACGCGGCGCCCGCTGGACGGCACGGCCATCGGGTTGATGGTGCTGCTGTGCGCCGC
CTGGGGGCTCCAGCAGGTGA

Product: glyoxalase I, Ni-dependent

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRLLHTMLRVGDYQRSIDFYTRVLGMTLLRESDNPEYKYRLAFVGYGPETETAVIELTYNYGVDKYDLGTAYGHIALEVP
SAAEACERIRSAGGKVTREAGPVKGGTTVIAFVEDPDGYKIELIERHSTRDAARP

Sequences:

>Translated_135_residues
MRLLHTMLRVGDYQRSIDFYTRVLGMTLLRESDNPEYKYRLAFVGYGPETETAVIELTYNYGVDKYDLGTAYGHIALEVP
SAAEACERIRSAGGKVTREAGPVKGGTTVIAFVEDPDGYKIELIERHSTRDAARP
>Mature_135_residues
MRLLHTMLRVGDYQRSIDFYTRVLGMTLLRESDNPEYKYRLAFVGYGPETETAVIELTYNYGVDKYDLGTAYGHIALEVP
SAAEACERIRSAGGKVTREAGPVKGGTTVIAFVEDPDGYKIELIERHSTRDAARP

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=36.986301369863, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=83.5820895522388, Blast_Score=227, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=33.0827067669173, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=37.410071942446, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=37.1621621621622, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920404, Length=138, Percent_Identity=34.0579710144928, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15094; Mature: 15094

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGDYQRSIDFYTRVLGMTLLRESDNPEYKYRLAFVGYGPETETAVIELTYN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEEEEC
YGVDKYDLGTAYGHIALEVPSAAEACERIRSAGGKVTREAGPVKGGTTVIAFVEDPDGYK
CCCCCEECCCCEEEEEEECCCHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCE
IELIERHSTRDAARP
EEEEECCCCCCCCCC
>Mature Secondary Structure
MRLLHTMLRVGDYQRSIDFYTRVLGMTLLRESDNPEYKYRLAFVGYGPETETAVIELTYN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEEEEC
YGVDKYDLGTAYGHIALEVPSAAEACERIRSAGGKVTREAGPVKGGTTVIAFVEDPDGYK
CCCCCEECCCCEEEEEEECCCHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCE
IELIERHSTRDAARP
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]