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The map label for this gene is ksgA

Identifier: 94309384

GI number: 94309384

Start: 465833

End: 466669

Strand: Direct

Name: ksgA

Synonym: Rmet_0439

Alternate gene names: 94309384

Gene position: 465833-466669 (Clockwise)

Preceding gene: 94309383

Following gene: 94309385

Centisome position: 11.86

GC content: 66.43

Gene sequence:

>837_bases
ATGCGTTCAAACGTGCACCAGGGCCATGTGGCCCGCAAACGGTTCGGACAGAACTTCCTCGTCGACGACGGCATCATTCA
CGGCATCGTCAGCGCGATTGACCCGCAGCCGAACGACATCGTGGTCGAGATCGGTCCGGGCCTCGGCGCGCTGACCGATC
CGCTGCTGGAGCGGCTGCCAGGCATGCAGGTGGTCGAGCTCGACCGGGATCTCGTGGAGCGGTTGCGTCGGCGCTACGGC
GACCGCCTCGTGGTCCACGCCGGCGATGCGCTGGCGTTCGATTTTGGCAAGCTGCGCGAGCCCGGCCGCGCGCTGCGCAT
CGTCGGTAACCTGCCGTACAACATTTCCAGCCCGCTGCTGTTCCATCTGGTGGATTTCGCCGATGACGTGCGCGACCAGC
ATTTCATGCTGCAGAAGGAAGTGGTCGAGCGCATGGTGGCCGACCCCGGCAGCAAATCGTATGGCCGCCTGTCGATCATG
CTGCAGGTTCGCTACCACATGGAGCACGTGCTCGATGTGCCGCCCGCGTCGTTCAATCCGCCGCCGAAGGTCGATTCCGC
CGTGGTGCGCATGATTCCGTGGCCACGCGCCGAGGATGGCACGCTGCGGTCGCCGTATGCCGCGTGCGATGCGGGCGTGT
TGGGCGACGTGGTGACCGCCGCGTTCTCGCAGCGCCGCAAGGTGCTGCGCAATACGCTGTCGTTCCTCCGCGACCAGGTT
GATTTCGACGCGCTAGGCTTCGATCTGACCCGCCGCGCCGAGGAAGTGCCCGTTGCCGAGTATGTCGAGCTCGCGCGAAT
CGTCGGTGGCGCGGAGCCGCCCGCCCGCGTGGCCTGA

Upstream 100 bases:

>100_bases
CGAAGCCATCCGCTGTGCCATTACCATGGCCGGACATGCCAGCGGGCGCCACAGCAATGGGCGCGCCGATAACGGCATCT
CCAACGGCCAGCACTGACTT

Downstream 100 bases:

>100_bases
GCCCCTTTTTATCCTGAATACCCTGAAGTCCTTGCGACGCCCATGACTGACCAGACCAACCCTGATAAGCGTCCCGTCAT
CCTGACTGGCGACCGTCCTA

Product: 16S ribosomal RNA methyltransferase KsgA/Dim1 family protein

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRSNVHQGHVARKRFGQNFLVDDGIIHGIVSAIDPQPNDIVVEIGPGLGALTDPLLERLPGMQVVELDRDLVERLRRRYG
DRLVVHAGDALAFDFGKLREPGRALRIVGNLPYNISSPLLFHLVDFADDVRDQHFMLQKEVVERMVADPGSKSYGRLSIM
LQVRYHMEHVLDVPPASFNPPPKVDSAVVRMIPWPRAEDGTLRSPYAACDAGVLGDVVTAAFSQRRKVLRNTLSFLRDQV
DFDALGFDLTRRAEEVPVAEYVELARIVGGAEPPARVA

Sequences:

>Translated_278_residues
MRSNVHQGHVARKRFGQNFLVDDGIIHGIVSAIDPQPNDIVVEIGPGLGALTDPLLERLPGMQVVELDRDLVERLRRRYG
DRLVVHAGDALAFDFGKLREPGRALRIVGNLPYNISSPLLFHLVDFADDVRDQHFMLQKEVVERMVADPGSKSYGRLSIM
LQVRYHMEHVLDVPPASFNPPPKVDSAVVRMIPWPRAEDGTLRSPYAACDAGVLGDVVTAAFSQRRKVLRNTLSFLRDQV
DFDALGFDLTRRAEEVPVAEYVELARIVGGAEPPARVA
>Mature_278_residues
MRSNVHQGHVARKRFGQNFLVDDGIIHGIVSAIDPQPNDIVVEIGPGLGALTDPLLERLPGMQVVELDRDLVERLRRRYG
DRLVVHAGDALAFDFGKLREPGRALRIVGNLPYNISSPLLFHLVDFADDVRDQHFMLQKEVVERMVADPGSKSYGRLSIM
LQVRYHMEHVLDVPPASFNPPPKVDSAVVRMIPWPRAEDGTLRSPYAACDAGVLGDVVTAAFSQRRKVLRNTLSFLRDQV
DFDALGFDLTRRAEEVPVAEYVELARIVGGAEPPARVA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=183, Percent_Identity=38.7978142076503, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI156415992, Length=249, Percent_Identity=31.7269076305221, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786236, Length=276, Percent_Identity=51.0869565217391, Blast_Score=271, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI25146882, Length=183, Percent_Identity=35.5191256830601, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=26.9360269360269, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=196, Percent_Identity=35.2040816326531, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358017, Length=197, Percent_Identity=37.0558375634518, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21357273, Length=282, Percent_Identity=29.7872340425532, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_RALME (Q1LRA1)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582594.1
- ProteinModelPortal:   Q1LRA1
- SMR:   Q1LRA1
- STRING:   Q1LRA1
- GeneID:   4037227
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0439
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q1LRA1
- ProtClustDB:   PRK00274
- BioCyc:   RMET266264:RMET_0439-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30927; Mature: 30927

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 89-89 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNVHQGHVARKRFGQNFLVDDGIIHGIVSAIDPQPNDIVVEIGPGLGALTDPLLERLP
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCC
GMQVVELDRDLVERLRRRYGDRLVVHAGDALAFDFGKLREPGRALRIVGNLPYNISSPLL
CCEEEHHHHHHHHHHHHHHCCEEEEEECCHHEECHHHHCCCCCEEEEEECCCCCCCCHHH
FHLVDFADDVRDQHFMLQKEVVERMVADPGSKSYGRLSIMLQVRYHMEHVLDVPPASFNP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCC
PPKVDSAVVRMIPWPRAEDGTLRSPYAACDAGVLGDVVTAAFSQRRKVLRNTLSFLRDQV
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DFDALGFDLTRRAEEVPVAEYVELARIVGGAEPPARVA
CHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRSNVHQGHVARKRFGQNFLVDDGIIHGIVSAIDPQPNDIVVEIGPGLGALTDPLLERLP
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCC
GMQVVELDRDLVERLRRRYGDRLVVHAGDALAFDFGKLREPGRALRIVGNLPYNISSPLL
CCEEEHHHHHHHHHHHHHHCCEEEEEECCHHEECHHHHCCCCCEEEEEECCCCCCCCHHH
FHLVDFADDVRDQHFMLQKEVVERMVADPGSKSYGRLSIMLQVRYHMEHVLDVPPASFNP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCC
PPKVDSAVVRMIPWPRAEDGTLRSPYAACDAGVLGDVVTAAFSQRRKVLRNTLSFLRDQV
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DFDALGFDLTRRAEEVPVAEYVELARIVGGAEPPARVA
CHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA