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The map label for this gene is paaF [H]

Identifier: 94309334

GI number: 94309334

Start: 417104

End: 417880

Strand: Direct

Name: paaF [H]

Synonym: Rmet_0389

Alternate gene names: 94309334

Gene position: 417104-417880 (Clockwise)

Preceding gene: 94309333

Following gene: 94309336

Centisome position: 10.62

GC content: 64.48

Gene sequence:

>777_bases
ATGAGCATTGCAACCAGCATCGACCAGGGCGTACTGACCATTGGCTTCGATCGCATCGACAAGAAGAACGCGATCACCGC
GGCGATGTACCAGACCATGGCCGACGCGCTGCGCGCCGCCGAGACCGACGCCGCGGTTCGCGTCATCGTGATCGAAGGCA
AGCCGGAAGTCTTCACGGCGGGCAACGACATCGAGGACTTCCTGAAGCGCCCGCCGACCAACGGCGCCGATGGCGCGCCG
GCACCGGTGTTCCAGTTCCTCCAGGCAATCAGCCGGGCGACCAAGCCCATCGTGGCATCGGTCAGCGGCGCAGCCGTTGG
CATCGGCACCACGCTGCTGCTGCATTGCGATCTCGTCTACGCATCCGAGACGGCAAAGCTGGCGCTGCCGTTCGCCCAAC
TCGGCCTCTGCCCGGAGGCGGCGTCGAGCCTGCTGTTGCCGCGCATGGTCGGCTATCAGCGCGCCGCCGAAAAGCTGCTT
CTTGGCGAGGCATTCAGCGCCCAGGAAGCCTTCGAAATCGGTCTGGTGACGAAGGTACTGTCGGTGGCGGAACTGCAGGG
TTATGTGCGTCAGCAGGCGGCCAAGCTGGCGGTGCTGCCCGCGTCGTCGCTGCGCGAAACCAAGCGCCTGATGAAGGGCG
ATGACGCGGCGGCCGTGGAAAAGAAGATGCTTGAGGAGGGCGAGGTGTTCCGCCGCATGCTGGTCGCGCCGGAAGCGAAG
GAGGCCTTTACGGCATTCTTCGAGAAGCGCAAGCCGGATTTCTCGAAGTTCAGCTAA

Upstream 100 bases:

>100_bases
CTGAACGTGGGAGCGGGCGCGTTCGGCTTGAGTTATCTTTCCGAAGCGCCGCCACCGTTCGTGTGATCGGCTTCCTTCTC
AAAACGGAGACGAGGTAGAA

Downstream 100 bases:

>100_bases
GGCGAAGCAAGCTGTAGCTTGTTTGCGCCCCTCTCCCATGCGATGGGAGAGGGGCTGCGGAGAGGAGAGAACTCCCTGCG
ATCAAGCCGCCGGCAACACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSIATSIDQGVLTIGFDRIDKKNAITAAMYQTMADALRAAETDAAVRVIVIEGKPEVFTAGNDIEDFLKRPPTNGADGAP
APVFQFLQAISRATKPIVASVSGAAVGIGTTLLLHCDLVYASETAKLALPFAQLGLCPEAASSLLLPRMVGYQRAAEKLL
LGEAFSAQEAFEIGLVTKVLSVAELQGYVRQQAAKLAVLPASSLRETKRLMKGDDAAAVEKKMLEEGEVFRRMLVAPEAK
EAFTAFFEKRKPDFSKFS

Sequences:

>Translated_258_residues
MSIATSIDQGVLTIGFDRIDKKNAITAAMYQTMADALRAAETDAAVRVIVIEGKPEVFTAGNDIEDFLKRPPTNGADGAP
APVFQFLQAISRATKPIVASVSGAAVGIGTTLLLHCDLVYASETAKLALPFAQLGLCPEAASSLLLPRMVGYQRAAEKLL
LGEAFSAQEAFEIGLVTKVLSVAELQGYVRQQAAKLAVLPASSLRETKRLMKGDDAAAVEKKMLEEGEVFRRMLVAPEAK
EAFTAFFEKRKPDFSKFS
>Mature_257_residues
SIATSIDQGVLTIGFDRIDKKNAITAAMYQTMADALRAAETDAAVRVIVIEGKPEVFTAGNDIEDFLKRPPTNGADGAPA
PVFQFLQAISRATKPIVASVSGAAVGIGTTLLLHCDLVYASETAKLALPFAQLGLCPEAASSLLLPRMVGYQRAAEKLLL
GEAFSAQEAFEIGLVTKVLSVAELQGYVRQQAAKLAVLPASSLRETKRLMKGDDAAAVEKKMLEEGEVFRRMLVAPEAKE
AFTAFFEKRKPDFSKFS

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=32.6693227091633, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=32.6693227091633, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=32.6693227091633, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4757968, Length=240, Percent_Identity=30, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI49355787, Length=240, Percent_Identity=30, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=31.7269076305221, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=28.75, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=28.75, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4757966, Length=229, Percent_Identity=29.2576419213974, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI51538581, Length=229, Percent_Identity=29.2576419213974, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI221136756, Length=241, Percent_Identity=25.3112033195021, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221136753, Length=241, Percent_Identity=25.3112033195021, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221307494, Length=241, Percent_Identity=25.3112033195021, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=31.6793893129771, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI116235487, Length=194, Percent_Identity=29.8969072164948, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI68989263, Length=168, Percent_Identity=32.7380952380952, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI70995211, Length=189, Percent_Identity=28.042328042328, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=25.8823529411765, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=31.7269076305221, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=29.9610894941634, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=30.078125, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=26.1904761904762, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788682, Length=164, Percent_Identity=28.0487804878049, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790281, Length=189, Percent_Identity=25.3968253968254, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=246, Percent_Identity=32.1138211382114, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=31.1740890688259, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=30.4878048780488, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17549921, Length=225, Percent_Identity=32.4444444444444, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=29.3532338308458, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=27.2222222222222, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=189, Percent_Identity=23.2804232804233, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=269, Percent_Identity=24.1635687732342, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=169, Percent_Identity=31.3609467455621, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=169, Percent_Identity=31.3609467455621, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=169, Percent_Identity=31.3609467455621, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24654903, Length=209, Percent_Identity=34.4497607655502, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=31.1023622047244, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=31.1023622047244, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=215, Percent_Identity=28.3720930232558, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=24.8062015503876, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650670, Length=203, Percent_Identity=29.5566502463054, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920382, Length=182, Percent_Identity=26.3736263736264, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27655; Mature: 27524

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIATSIDQGVLTIGFDRIDKKNAITAAMYQTMADALRAAETDAAVRVIVIEGKPEVFTA
CCCCCCCCCCEEEECHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEC
GNDIEDFLKRPPTNGADGAPAPVFQFLQAISRATKPIVASVSGAAVGIGTTLLLHCDLVY
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHE
ASETAKLALPFAQLGLCPEAASSLLLPRMVGYQRAAEKLLLGEAFSAQEAFEIGLVTKVL
ECCCHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAELQGYVRQQAAKLAVLPASSLRETKRLMKGDDAAAVEKKMLEEGEVFRRMLVAPEAK
HHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
EAFTAFFEKRKPDFSKFS
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SIATSIDQGVLTIGFDRIDKKNAITAAMYQTMADALRAAETDAAVRVIVIEGKPEVFTA
CCCCCCCCCEEEECHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEC
GNDIEDFLKRPPTNGADGAPAPVFQFLQAISRATKPIVASVSGAAVGIGTTLLLHCDLVY
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHE
ASETAKLALPFAQLGLCPEAASSLLLPRMVGYQRAAEKLLLGEAFSAQEAFEIGLVTKVL
ECCCHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAELQGYVRQQAAKLAVLPASSLRETKRLMKGDDAAAVEKKMLEEGEVFRRMLVAPEAK
HHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
EAFTAFFEKRKPDFSKFS
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]