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The map label for this gene is ubiB [H]

Identifier: 94309319

GI number: 94309319

Start: 398504

End: 400081

Strand: Direct

Name: ubiB [H]

Synonym: Rmet_0374

Alternate gene names: 94309319

Gene position: 398504-400081 (Clockwise)

Preceding gene: 94309318

Following gene: 335055487

Centisome position: 10.14

GC content: 64.58

Gene sequence:

>1578_bases
ATGACCCGCCTGCTGCGTCTCTGCAAGATACTGTTTGTCATCCTGTACTACGGGCTCGATGAACTCGTGCTGTCCGGCTT
CAAGAACCGGCGCATCAAGTTCCTCGTCCGAGTCATCACCATCGGCCGCAAGCTCGATCAGCCGCGCGGCGAACGCCTGC
GTATGGCGCTGACTGCGCTGGGCCCGATCTTCGTCAAGTTCGGCCAGGTGCTGTCCACGCGCCGTGACCTGATGCCGCCC
GATATCGCCGACGAACTGGCCAAGCTGCAGGACCAGGTGCCGCCGTTCGAGTCAGCGGTGGCGGTGAAGATCATCGAGAA
ATCGCTGGGCCGCCGGCTGACGGAACTGTTCGAGACATTCGAGCACGAGCCCGTGGCCAGCGCGTCGATCGCCCAGGTTC
ACTTCGCCACGCTGCGCGGCGGTCCGAGCCATGGCCGCGAGGTGGCCGTGAAGGTGCTGCGTCCGGGCATGTTGCCGGTA
ATCGATAGCGACCTGGCGCTGATGCGCGACATGGCCACGTGGCTCGAGCGCGTCTGGGTGGACGGTCGCCGGCTCAAGCC
CCGCGAGGTGGTGGCGGAGTTCGACAAGTACCTGCACGACGAACTCGACCTGATGATCGAGGCCGCCAATGCCAGCCAGT
TGCGCCGCAACTTTGCCGAAAGCGACCTGCTTTGCGTGCCCGAGGTGTTCTGGGACTGGTGCACGAGCAGCGTCTTCACG
ATGGAGCGGATGCATGGCATTCCGATCTCCCGCACCGAATCGCTGAAGGCGGCGGGCGTCGACATGCACAAGCTGGCCGA
AGAGGGCGTCGAGATCTTCTTCACGCAGGTGTTCCGCGACGGGTTCTTCCACGCGGACATGCACCCCGGCAACATCCTGG
TGTCGGTCAGACCCGAGACGTTCGGCCGGTATATCGCGCTGGACTTCGGCATCGTCGGCGCGCTGTCCGAGTTCGACAAA
AACTACCTGGCGCAGAACTTCATCGCGTTTTTCCGCCGCGACTACCATCGTGTGGCGCTCCTGCACGTGGAGTCGGGCTG
GGTGCCGCCGGAGACCCGGGTCGAGGAACTCGAATCCGCCGTGCGTGCCTGCTGCGAACCGTACTTCGACAAACCGCTGC
GCGAGATTTCGCTCGGCATGGTGCTGATGCGGTTGTTCCAGACCTCGCGCCGCTTCAACGTCGAGATCCAGCCGCAACTT
GTGCTGCTGCAGAAGACGCTGCTCAATATCGAAGGCCTGGGCCGCCAGCTTGACCCGGACCTCGACCTCTGGAAGACCGC
CAAGCCCTTCCTGGAACGCTGGATGTACGAGCAGGTGGGCTGGCAGGGCGCCTGGGAGCGGATCAAGATCGAGGCGCCGC
AATGGGCGAAGATGTTGCCCGACTTTCCCCGGCTGGCCCACCAGTTCCTGGAGCGCCGCGCGCTGCAGAACAACGAAGGG
CAGGAGCGTCTGCTTACCCAACTGGTGGCCGAGCAGCGCCGCACGAACCGCCGGCTGTCCACGGCCCTGCTGCTGATGGG
CGGGTTCCTGGCCGGCATCGTGATCGTCCAGGCACTGGCCTGGGCGGGATACTGGTAG

Upstream 100 bases:

>100_bases
GGCTGGAGCGTGCTCCGGGCGCGGGCTCCCCGGATGCCTTACCCTCGGCCCATCGCTGACCTGCCCTGACCCGCCACGTG
TCGTTTCCTGAAGATTTCGC

Downstream 100 bases:

>100_bases
TCACCGCGCCTGAAGCGTCGCTGGTTTCCCCTTTCTCCCGCAGGCGGTGGGAGGGGAGAACCACCATACAACACCCAAGT
GAACCAAGATGGGGATTTGC

Product: putative ubiquinone biosynthesis protein UbiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTRLLRLCKILFVILYYGLDELVLSGFKNRRIKFLVRVITIGRKLDQPRGERLRMALTALGPIFVKFGQVLSTRRDLMPP
DIADELAKLQDQVPPFESAVAVKIIEKSLGRRLTELFETFEHEPVASASIAQVHFATLRGGPSHGREVAVKVLRPGMLPV
IDSDLALMRDMATWLERVWVDGRRLKPREVVAEFDKYLHDELDLMIEAANASQLRRNFAESDLLCVPEVFWDWCTSSVFT
MERMHGIPISRTESLKAAGVDMHKLAEEGVEIFFTQVFRDGFFHADMHPGNILVSVRPETFGRYIALDFGIVGALSEFDK
NYLAQNFIAFFRRDYHRVALLHVESGWVPPETRVEELESAVRACCEPYFDKPLREISLGMVLMRLFQTSRRFNVEIQPQL
VLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMYEQVGWQGAWERIKIEAPQWAKMLPDFPRLAHQFLERRALQNNEG
QERLLTQLVAEQRRTNRRLSTALLLMGGFLAGIVIVQALAWAGYW

Sequences:

>Translated_525_residues
MTRLLRLCKILFVILYYGLDELVLSGFKNRRIKFLVRVITIGRKLDQPRGERLRMALTALGPIFVKFGQVLSTRRDLMPP
DIADELAKLQDQVPPFESAVAVKIIEKSLGRRLTELFETFEHEPVASASIAQVHFATLRGGPSHGREVAVKVLRPGMLPV
IDSDLALMRDMATWLERVWVDGRRLKPREVVAEFDKYLHDELDLMIEAANASQLRRNFAESDLLCVPEVFWDWCTSSVFT
MERMHGIPISRTESLKAAGVDMHKLAEEGVEIFFTQVFRDGFFHADMHPGNILVSVRPETFGRYIALDFGIVGALSEFDK
NYLAQNFIAFFRRDYHRVALLHVESGWVPPETRVEELESAVRACCEPYFDKPLREISLGMVLMRLFQTSRRFNVEIQPQL
VLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMYEQVGWQGAWERIKIEAPQWAKMLPDFPRLAHQFLERRALQNNEG
QERLLTQLVAEQRRTNRRLSTALLLMGGFLAGIVIVQALAWAGYW
>Mature_524_residues
TRLLRLCKILFVILYYGLDELVLSGFKNRRIKFLVRVITIGRKLDQPRGERLRMALTALGPIFVKFGQVLSTRRDLMPPD
IADELAKLQDQVPPFESAVAVKIIEKSLGRRLTELFETFEHEPVASASIAQVHFATLRGGPSHGREVAVKVLRPGMLPVI
DSDLALMRDMATWLERVWVDGRRLKPREVVAEFDKYLHDELDLMIEAANASQLRRNFAESDLLCVPEVFWDWCTSSVFTM
ERMHGIPISRTESLKAAGVDMHKLAEEGVEIFFTQVFRDGFFHADMHPGNILVSVRPETFGRYIALDFGIVGALSEFDKN
YLAQNFIAFFRRDYHRVALLHVESGWVPPETRVEELESAVRACCEPYFDKPLREISLGMVLMRLFQTSRRFNVEIQPQLV
LLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMYEQVGWQGAWERIKIEAPQWAKMLPDFPRLAHQFLERRALQNNEGQ
ERLLTQLVAEQRRTNRRLSTALLLMGGFLAGIVIVQALAWAGYW

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI41393593, Length=326, Percent_Identity=28.5276073619632, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI40254938, Length=261, Percent_Identity=30.6513409961686, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI217035081, Length=216, Percent_Identity=31.0185185185185, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI32261307, Length=316, Percent_Identity=27.8481012658228, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI34147522, Length=260, Percent_Identity=27.6923076923077, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI27363457, Length=286, Percent_Identity=25.1748251748252, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI2367309, Length=542, Percent_Identity=50.7380073800738, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17559152, Length=296, Percent_Identity=28.0405405405405, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32565180, Length=268, Percent_Identity=27.2388059701493, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325148, Length=441, Percent_Identity=26.984126984127, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323282, Length=249, Percent_Identity=27.3092369477912, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321319, Length=265, Percent_Identity=26.7924528301887, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI22024280, Length=268, Percent_Identity=29.8507462686567, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24662575, Length=277, Percent_Identity=29.9638989169675, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI18859849, Length=281, Percent_Identity=26.6903914590747, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 60505; Mature: 60374

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLLRLCKILFVILYYGLDELVLSGFKNRRIKFLVRVITIGRKLDQPRGERLRMALTAL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GPIFVKFGQVLSTRRDLMPPDIADELAKLQDQVPPFESAVAVKIIEKSLGRRLTELFETF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EHEPVASASIAQVHFATLRGGPSHGREVAVKVLRPGMLPVIDSDLALMRDMATWLERVWV
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHHC
DGRRLKPREVVAEFDKYLHDELDLMIEAANASQLRRNFAESDLLCVPEVFWDWCTSSVFT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
MERMHGIPISRTESLKAAGVDMHKLAEEGVEIFFTQVFRDGFFHADMHPGNILVSVRPET
HHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHH
FGRYIALDFGIVGALSEFDKNYLAQNFIAFFRRDYHRVALLHVESGWVPPETRVEELESA
HCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCHHHHHHHHHH
VRACCEPYFDKPLREISLGMVLMRLFQTSRRFNVEIQPQLVLLQKTLLNIEGLGRQLDPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCC
LDLWKTAKPFLERWMYEQVGWQGAWERIKIEAPQWAKMLPDFPRLAHQFLERRALQNNEG
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCC
QERLLTQLVAEQRRTNRRLSTALLLMGGFLAGIVIVQALAWAGYW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRLLRLCKILFVILYYGLDELVLSGFKNRRIKFLVRVITIGRKLDQPRGERLRMALTAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GPIFVKFGQVLSTRRDLMPPDIADELAKLQDQVPPFESAVAVKIIEKSLGRRLTELFETF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EHEPVASASIAQVHFATLRGGPSHGREVAVKVLRPGMLPVIDSDLALMRDMATWLERVWV
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHHC
DGRRLKPREVVAEFDKYLHDELDLMIEAANASQLRRNFAESDLLCVPEVFWDWCTSSVFT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
MERMHGIPISRTESLKAAGVDMHKLAEEGVEIFFTQVFRDGFFHADMHPGNILVSVRPET
HHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHH
FGRYIALDFGIVGALSEFDKNYLAQNFIAFFRRDYHRVALLHVESGWVPPETRVEELESA
HCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCHHHHHHHHHH
VRACCEPYFDKPLREISLGMVLMRLFQTSRRFNVEIQPQLVLLQKTLLNIEGLGRQLDPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCC
LDLWKTAKPFLERWMYEQVGWQGAWERIKIEAPQWAKMLPDFPRLAHQFLERRALQNNEG
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCC
QERLLTQLVAEQRRTNRRLSTALLLMGGFLAGIVIVQALAWAGYW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]