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The map label for this gene is otsB [H]

Identifier: 94309300

GI number: 94309300

Start: 375533

End: 376351

Strand: Direct

Name: otsB [H]

Synonym: Rmet_0355

Alternate gene names: 94309300

Gene position: 375533-376351 (Clockwise)

Preceding gene: 94309297

Following gene: 94309301

Centisome position: 9.56

GC content: 67.03

Gene sequence:

>819_bases
GTGTTACGGTCTTGCGTGATGTCTTCGCTACCTCTCATCGAACCCGATACCGCGCTGTTTCTGGATTTCGACGGCACGCT
GGCTGACCTCGCGCCACGCCCCGAGCTGGTGCAGGTCGAGCCGGAACTGGTTGGCACGCTGCGCGCGCTGCACACCTGCC
TGGACGGCGCCCTGGCCATTATCTCGGGCCGGCCCGTTGCCGAGCTCGATGGCTTCCTGCAGCCGCTGAGGCTCCCCGCG
GCAGGCGTGCACGGCGCCGAACTGCGCCATGACGGCGAAGCGCTGTTCATGCCACCGGCCCCGGGCATCACGGCATTGAT
GCCGAGGCTCGAAGCCTTCGTCGCGCGCCATCCCGGCCTACGCATCGAGCGGAAATCCGTGGCAGTCGCCATTCACTATC
GACAGGCTCCGGAACTCGAGGCACAGGTGCGAGCGATGGTGGCCGACACCCTGCACGACGTGGATGGGCTGGAAGGCTTG
CCCGGAAAAATGGTTGTCGAGATCAAGCCTGCCGGCGTCGACAAGGGGGGCGCGATCGCCGCGTTCATGCGCATGGCCCC
CTTCGAAGGACGCAAGCCGCTGTTCGCGGGTGACGATGTCACCGACGAAGCCGGTTTCGTTGTGGCGCGTGAACTCGGCG
GGGTGGGCGTGCTGGTTGGCGAGCGCGCCACCGCCGCCACGGTCAGCGTGACCGGGCCCGCTGCGTTGCGTTGCTGGCTT
CATCGATCCGCCCATGCTCTTCTGGCGCTCAGGGCGCAACGCGGCGGCAAGCCGCAGTCCGTCTCATCCACTTCGTCCGA
AACGGAGGACTCGCTGTGA

Upstream 100 bases:

>100_bases
ATGGCGCACTTGGCAGTGCCAGGTTGGCAGTTGCCGTGCGACGGGTGCAGTGACAGCCACAGTTGTAGGACAGCGTCCGG
CTGACACCTTCCAAATCGAC

Downstream 100 bases:

>100_bases
ACGCCCCCCCCACACCTGCCGCGCATCATCCAGAAGCGCCCGCTTTCAATACCGATACCAACTGCACTGGCCGTCACGCC
GATCCGTCGCTATCGCTCGG

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLRSCVMSSLPLIEPDTALFLDFDGTLADLAPRPELVQVEPELVGTLRALHTCLDGALAIISGRPVAELDGFLQPLRLPA
AGVHGAELRHDGEALFMPPAPGITALMPRLEAFVARHPGLRIERKSVAVAIHYRQAPELEAQVRAMVADTLHDVDGLEGL
PGKMVVEIKPAGVDKGGAIAAFMRMAPFEGRKPLFAGDDVTDEAGFVVARELGGVGVLVGERATAATVSVTGPAALRCWL
HRSAHALLALRAQRGGKPQSVSSTSSETEDSL

Sequences:

>Translated_272_residues
MLRSCVMSSLPLIEPDTALFLDFDGTLADLAPRPELVQVEPELVGTLRALHTCLDGALAIISGRPVAELDGFLQPLRLPA
AGVHGAELRHDGEALFMPPAPGITALMPRLEAFVARHPGLRIERKSVAVAIHYRQAPELEAQVRAMVADTLHDVDGLEGL
PGKMVVEIKPAGVDKGGAIAAFMRMAPFEGRKPLFAGDDVTDEAGFVVARELGGVGVLVGERATAATVSVTGPAALRCWL
HRSAHALLALRAQRGGKPQSVSSTSSETEDSL
>Mature_272_residues
MLRSCVMSSLPLIEPDTALFLDFDGTLADLAPRPELVQVEPELVGTLRALHTCLDGALAIISGRPVAELDGFLQPLRLPA
AGVHGAELRHDGEALFMPPAPGITALMPRLEAFVARHPGLRIERKSVAVAIHYRQAPELEAQVRAMVADTLHDVDGLEGL
PGKMVVEIKPAGVDKGGAIAAFMRMAPFEGRKPLFAGDDVTDEAGFVVARELGGVGVLVGERATAATVSVTGPAALRCWL
HRSAHALLALRAQRGGKPQSVSSTSSETEDSL

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=247, Percent_Identity=41.7004048582996, Blast_Score=178, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19920676, Length=203, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221473368, Length=220, Percent_Identity=28.6363636363636, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20129307, Length=220, Percent_Identity=28.6363636363636, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582490, Length=220, Percent_Identity=28.6363636363636, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28707; Mature: 28707

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSCVMSSLPLIEPDTALFLDFDGTLADLAPRPELVQVEPELVGTLRALHTCLDGALAI
CCHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEEE
ISGRPVAELDGFLQPLRLPAAGVHGAELRHDGEALFMPPAPGITALMPRLEAFVARHPGL
ECCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCC
RIERKSVAVAIHYRQAPELEAQVRAMVADTLHDVDGLEGLPGKMVVEIKPAGVDKGGAIA
EEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHH
AFMRMAPFEGRKPLFAGDDVTDEAGFVVARELGGVGVLVGERATAATVSVTGPAALRCWL
HHHHHCCCCCCCCEECCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCHHHHHHHH
HRSAHALLALRAQRGGKPQSVSSTSSETEDSL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRSCVMSSLPLIEPDTALFLDFDGTLADLAPRPELVQVEPELVGTLRALHTCLDGALAI
CCHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEEE
ISGRPVAELDGFLQPLRLPAAGVHGAELRHDGEALFMPPAPGITALMPRLEAFVARHPGL
ECCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCC
RIERKSVAVAIHYRQAPELEAQVRAMVADTLHDVDGLEGLPGKMVVEIKPAGVDKGGAIA
EEEECEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHH
AFMRMAPFEGRKPLFAGDDVTDEAGFVVARELGGVGVLVGERATAATVSVTGPAALRCWL
HHHHHCCCCCCCCEECCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEEECCHHHHHHHH
HRSAHALLALRAQRGGKPQSVSSTSSETEDSL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]