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The map label for this gene is mhpA [H]

Identifier: 94309284

GI number: 94309284

Start: 356913

End: 358667

Strand: Direct

Name: mhpA [H]

Synonym: Rmet_0339

Alternate gene names: 94309284

Gene position: 356913-358667 (Clockwise)

Preceding gene: 94309283

Following gene: 94309285

Centisome position: 9.09

GC content: 66.78

Gene sequence:

>1755_bases
ATGAGCAGTACCAAGGTCGACATCGACTACCAGCGCATCCAGTACGACTACGTACCATGCGCCGAGCAGCGGGGGGCATT
GACCAATGCCGCCCCGCATCCCGTGATCGTGGTCGGGGCCGGGCCGATTGGCCTGGCCACCGCCATCGACCTGGCCCGCC
ACGGCGTGCCGGTGGTGGTGGTCGATGACGACTGCATGCTCTCGGTCGGATCGCGTGCAATCTGCTTCGCCAAGCGCACG
CTGGATATCTTCGACCGGCTCGGCTGTGGCGACCGCATGGTCGACAAGGGCGTGCGCTGGAACCTTGGCAAGGTCTTCCT
GCGGGACAGCCAGGTCTATGCGTTCGACCTGTTGCCCGAATCCGACCACCAGCGGCCGGCGTTTATCAACCTGCAGCAGT
ACTACCTCGAGGGCTATCTGCTCGAATGCGCGCAGGCGATGCCCAACATCGAGATCCGCTGGAAGAGCAAGGCAGTTGCC
ATCGAGCAACACGCGGACCACGTGACGCTGACGATCGAAACGCCCGATGGCTGTTACGCGCTGCGCGGTGCCTACGTGGT
GGCCGCCGATGGCTCGCGCAGCCCGCTGCGCAAGATGCTGGGTCTGGAGAGCCACGGTCATACGTTCCGAGACCGCTTCC
TGATCGCCGACGTGAAGATGGAAGCGCCGTTCCCGGCCGAGCGCTGGTTCTGGTTTGATCCGCCGTTCCATCCGAACCAG
TCGGTGCTGCTGCACCGGCAGCCTGACAACGTCTGGCGCATTGACTTCCAGCTCGGGTGGGATGCCGATCCCGCATTCGA
GAAGACGCCCGAGCGTGTGCTGCCGCGTGTACAGGCGCTGCTTGGCCCCGATGCGAAGTTCGAGCTCGAATGGGTCAGCG
TCTATACGTTCTCGTGCGTGCGGATGGACAGCTTCCGCCACGGCCGCGTGTTGTTCGCGGGCGATTCGGCCCACTGCGTC
TCGCCGTTCGGCGCGCGCGGCGCTAACAGCGGCGTGCAGGATGCCGAGAACCTTGCCTGGAAACTGGCCTACGTGCTGCA
GGGCAAGGCGCCGGAAAGCCTGCTGGATACCTACGCCAGCGAGCGCGAGTTCGCGGCCGACGAGAACATCCTCAACTCGA
CGCGCTCGACCGATTTCATTACGCCGAAGAGCGCGGTCAGCCGCATCTTCCGCGACGCGGTGCTGACACTGGCGCGCGAC
CACGCGTTTGCGCGGCAACTGGTCAATAGTGGCCGGCTGTCGGTGCCGGCCGTGCTGCGCGACTCGCCGCTGAACACCGC
CGATGCGGAATCGTTCTCCGGCCAGATGGTGCCGGGCGCAGCATGCGTCGATGCCCCCGTGGCCGGCACGGAAGGCAAGC
CCTGGTTGCTGCCGCATCTGGGCGACGACTTCGTGGCGCTGATCTTTGGCGCGCCGGCTGGCATCGACGCAGCATGCCGT
GAAGCGCTGACGCAACTGCTGCAGGGAGACATTCCGCTGCGGGTGGTGTTCGTGACCGATGCGGCCATTTCGGCATCGGC
CGAAGCCTGGCCCGGCGCGGTCGTGCTGCGCGACGCGGGCGGCGTGGCGGCCCGGCGCTATGACGCGCGCGCGGGCACCT
GCTACCTGATTCGCCCTGACCAGCACGTCTGCGCGCGGTGGCGCGCGGTCGATGCGAACCAGATCGCCGGAGCGATCGCT
CGCGCCACATGCAATGCCGCAGCAACCGTCGCGGCGCAATCGCCAGCCGCGGCACGCACGCCGGCACTGGCCTGA

Upstream 100 bases:

>100_bases
TTGTTCGGTTGTTGTGTGTGGTTTCCCTCTCCCATGAAGTGGGAGAAGGGAGCAAACAGAAAGCAGTATCAAGCGCCGCA
AGTGCGTGGATGGAGACACC

Downstream 100 bases:

>100_bases
TCGACAGTCACCAGGAGACAGGCCCATGCCGCTCAACACCGCTCCCAATCTTGCCTATCCCGACGAGTTCTACGAGGCGC
TCATCGAGATGCATCATGAC

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSSTKVDIDYQRIQYDYVPCAEQRGALTNAAPHPVIVVGAGPIGLATAIDLARHGVPVVVVDDDCMLSVGSRAICFAKRT
LDIFDRLGCGDRMVDKGVRWNLGKVFLRDSQVYAFDLLPESDHQRPAFINLQQYYLEGYLLECAQAMPNIEIRWKSKAVA
IEQHADHVTLTIETPDGCYALRGAYVVAADGSRSPLRKMLGLESHGHTFRDRFLIADVKMEAPFPAERWFWFDPPFHPNQ
SVLLHRQPDNVWRIDFQLGWDADPAFEKTPERVLPRVQALLGPDAKFELEWVSVYTFSCVRMDSFRHGRVLFAGDSAHCV
SPFGARGANSGVQDAENLAWKLAYVLQGKAPESLLDTYASEREFAADENILNSTRSTDFITPKSAVSRIFRDAVLTLARD
HAFARQLVNSGRLSVPAVLRDSPLNTADAESFSGQMVPGAACVDAPVAGTEGKPWLLPHLGDDFVALIFGAPAGIDAACR
EALTQLLQGDIPLRVVFVTDAAISASAEAWPGAVVLRDAGGVAARRYDARAGTCYLIRPDQHVCARWRAVDANQIAGAIA
RATCNAAATVAAQSPAAARTPALA

Sequences:

>Translated_584_residues
MSSTKVDIDYQRIQYDYVPCAEQRGALTNAAPHPVIVVGAGPIGLATAIDLARHGVPVVVVDDDCMLSVGSRAICFAKRT
LDIFDRLGCGDRMVDKGVRWNLGKVFLRDSQVYAFDLLPESDHQRPAFINLQQYYLEGYLLECAQAMPNIEIRWKSKAVA
IEQHADHVTLTIETPDGCYALRGAYVVAADGSRSPLRKMLGLESHGHTFRDRFLIADVKMEAPFPAERWFWFDPPFHPNQ
SVLLHRQPDNVWRIDFQLGWDADPAFEKTPERVLPRVQALLGPDAKFELEWVSVYTFSCVRMDSFRHGRVLFAGDSAHCV
SPFGARGANSGVQDAENLAWKLAYVLQGKAPESLLDTYASEREFAADENILNSTRSTDFITPKSAVSRIFRDAVLTLARD
HAFARQLVNSGRLSVPAVLRDSPLNTADAESFSGQMVPGAACVDAPVAGTEGKPWLLPHLGDDFVALIFGAPAGIDAACR
EALTQLLQGDIPLRVVFVTDAAISASAEAWPGAVVLRDAGGVAARRYDARAGTCYLIRPDQHVCARWRAVDANQIAGAIA
RATCNAAATVAAQSPAAARTPALA
>Mature_583_residues
SSTKVDIDYQRIQYDYVPCAEQRGALTNAAPHPVIVVGAGPIGLATAIDLARHGVPVVVVDDDCMLSVGSRAICFAKRTL
DIFDRLGCGDRMVDKGVRWNLGKVFLRDSQVYAFDLLPESDHQRPAFINLQQYYLEGYLLECAQAMPNIEIRWKSKAVAI
EQHADHVTLTIETPDGCYALRGAYVVAADGSRSPLRKMLGLESHGHTFRDRFLIADVKMEAPFPAERWFWFDPPFHPNQS
VLLHRQPDNVWRIDFQLGWDADPAFEKTPERVLPRVQALLGPDAKFELEWVSVYTFSCVRMDSFRHGRVLFAGDSAHCVS
PFGARGANSGVQDAENLAWKLAYVLQGKAPESLLDTYASEREFAADENILNSTRSTDFITPKSAVSRIFRDAVLTLARDH
AFARQLVNSGRLSVPAVLRDSPLNTADAESFSGQMVPGAACVDAPVAGTEGKPWLLPHLGDDFVALIFGAPAGIDAACRE
ALTQLLQGDIPLRVVFVTDAAISASAEAWPGAVVLRDAGGVAARRYDARAGTCYLIRPDQHVCARWRAVDANQIAGAIAR
ATCNAAATVAAQSPAAARTPALA

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=365, Percent_Identity=30.1369863013699, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 63698; Mature: 63567

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTKVDIDYQRIQYDYVPCAEQRGALTNAAPHPVIVVGAGPIGLATAIDLARHGVPVVV
CCCCEEEEEHEEEEEECCCCHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEE
VDDDCMLSVGSRAICFAKRTLDIFDRLGCGDRMVDKGVRWNLGKVFLRDSQVYAFDLLPE
ECCCCEEECCCCEEEEHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCEEEEEEECCC
SDHQRPAFINLQQYYLEGYLLECAQAMPNIEIRWKSKAVAIEQHADHVTLTIETPDGCYA
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCEEEEEEECCCCCEE
LRGAYVVAADGSRSPLRKMLGLESHGHTFRDRFLIADVKMEAPFPAERWFWFDPPFHPNQ
ECCCEEEEECCCHHHHHHHHCCCCCCCCHHCEEEEEEEEECCCCCCCCEEEECCCCCCCC
SVLLHRQPDNVWRIDFQLGWDADPAFEKTPERVLPRVQALLGPDAKFELEWVSVYTFSCV
CEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEE
RMDSFRHGRVLFAGDSAHCVSPFGARGANSGVQDAENLAWKLAYVLQGKAPESLLDTYAS
EECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHHC
EREFAADENILNSTRSTDFITPKSAVSRIFRDAVLTLARDHAFARQLVNSGRLSVPAVLR
CCHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
DSPLNTADAESFSGQMVPGAACVDAPVAGTEGKPWLLPHLGDDFVALIFGAPAGIDAACR
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCHHHH
EALTQLLQGDIPLRVVFVTDAAISASAEAWPGAVVLRDAGGVAARRYDARAGTCYLIRPD
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEEECCC
QHVCARWRAVDANQIAGAIARATCNAAATVAAQSPAAARTPALA
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SSTKVDIDYQRIQYDYVPCAEQRGALTNAAPHPVIVVGAGPIGLATAIDLARHGVPVVV
CCCEEEEEHEEEEEECCCCHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEE
VDDDCMLSVGSRAICFAKRTLDIFDRLGCGDRMVDKGVRWNLGKVFLRDSQVYAFDLLPE
ECCCCEEECCCCEEEEHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCEEEEEEECCC
SDHQRPAFINLQQYYLEGYLLECAQAMPNIEIRWKSKAVAIEQHADHVTLTIETPDGCYA
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCEEEEEEECCCCCEE
LRGAYVVAADGSRSPLRKMLGLESHGHTFRDRFLIADVKMEAPFPAERWFWFDPPFHPNQ
ECCCEEEEECCCHHHHHHHHCCCCCCCCHHCEEEEEEEEECCCCCCCCEEEECCCCCCCC
SVLLHRQPDNVWRIDFQLGWDADPAFEKTPERVLPRVQALLGPDAKFELEWVSVYTFSCV
CEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEE
RMDSFRHGRVLFAGDSAHCVSPFGARGANSGVQDAENLAWKLAYVLQGKAPESLLDTYAS
EECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHHC
EREFAADENILNSTRSTDFITPKSAVSRIFRDAVLTLARDHAFARQLVNSGRLSVPAVLR
CCHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
DSPLNTADAESFSGQMVPGAACVDAPVAGTEGKPWLLPHLGDDFVALIFGAPAGIDAACR
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCHHHH
EALTQLLQGDIPLRVVFVTDAAISASAEAWPGAVVLRDAGGVAARRYDARAGTCYLIRPD
HHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEEECCC
QHVCARWRAVDANQIAGAIARATCNAAATVAAQSPAAARTPALA
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA