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The map label for this gene is mltF [H]

Identifier: 94309280

GI number: 94309280

Start: 352361

End: 353860

Strand: Reverse

Name: mltF [H]

Synonym: Rmet_0335

Alternate gene names: 94309280

Gene position: 353860-352361 (Counterclockwise)

Preceding gene: 94309281

Following gene: 94309279

Centisome position: 9.01

GC content: 60.87

Gene sequence:

>1500_bases
ATGGTCAACGATCGAAGGCGGTTCCTTGTCATCGGCGCCCGCGCACTGGCCGGCGCTTCCATCACGTGGTCAGGACTGGC
GGCGGCAGCGACGCCCGCCATTCCAGCTCCCAAGGAAAAACGGCTCACCCCTCCGCGCCAGCTCAACCTGACCAACAAAC
CCTGGACCGGTGACTTCGATGGCATGTTCAAGCACCGCACCATTCGCGTGCTGGTGCCCTACAGCCGCACCCTGTACTTC
AACGACCGGGGACGCGAGCGGGGCGTCACGGCGGACACCGTGCGGGATTTCGAGCGCTATATCAACAAGACCATGGCCGC
TCAGCTTGCCAAGCGGCCGCTCACGGTCTACATGATTCCGGTCACGCGCGACCGTTTGCTGCCCGATCTCAATGCCGGTC
TTGGCGACATCGCAGCCGGCAATCTCACGGTCACCGACTCCCGGCTCAAGCAAGCGGACTTCATTGTCGGGCAGACCAGC
AAGCCGATCCGGGAGCTGGTCATCACGGGCCCGAAATCCCCCACGCTTGCCACGCTCGACGACCTGGCTGGCAAGGCCGT
GCACGTGCGCCCCAGCAGCAGCTACTACGAAAGCCTGACCGCGTTGAACGACCGCTTCCGTAAGGCCGGCAAACCGCAGA
TGCACCTGGTGCTGCTACCCGACGCGCTGGAGGACGAAGACGCACTGGAAATGCTCAACGCCGGCACGCTGCAGATCCTG
GTGGTCGACGACTGGAAGGCGCGCATCTGGGCCCAGATCCTGAAGCGCATCAAGGTTCGCGATGACCTCGCGGTACGTGA
AGGCAGCAAGATCGGCTGGGCAATCCGCAAAGGCAGTCCAAAGCTGAACGCGGTCCTCGTGGACTACAACGACAACTTCA
TCAAGAAGCAGGGCATCGCCGACTACCGGCTGAAGCAATACATGAAGGTCATCAAGCAGATCTCGGACAACACCAACGGC
GCGGAGATCAAGCGGTTCCAGGACACCCTGGCGTTCTTCGAGAAGTACGGCAAGCTCTACCATTTCGATCCGCTGATGCT
GGCGGCGCAGGGCTTCCAGGAGTCCCAGCTCAATCAGAACGCGCGTAGCCATGTGGGAGCGATCGGTGTCATGCAGATCA
TGCCGGCCACTGGGAGGGAACTGAACGTCGGCAGCATCAAGATCACCGAATCCAATATCCACGCGGGTACCAAATACCTG
GACCAGCTGATGACGAGGTACTTCTCCGATGCGAATTTCTCGGAATCTGACCGGCCACTGTTTGCATTCGCCAGCTACAA
CGCCGGGCCGGGAAATATCTCCAAGATGCGCACCGAAGCCAAGGCTCGGGGCCTGAACCCGGACAAGTGGTTCAACAACG
TCGAGATCGTGACCTCCGAGCGGATCGGCATCGAGACCACCACCTACGTGCGCAACATCTACAAGTACTACGCCGCCTAC
AAGCTGATCGAGGATGCGCAGGAAGAACGAAGCCGCGCCATGAAGAAGGTAGCCGGGTAA

Upstream 100 bases:

>100_bases
CTATGCTTAGTCAACGGACGGCGATTGCCTGCGCATCCGGGCCCGCTCCCGGTGGCCGGCATCCGCGTGCCAGCAGGACC
TGACTGTCGCGAGGTGTGCA

Downstream 100 bases:

>100_bases
AGGCTGGCCACAGCACCGGGTCTGGTGCCCGGCTCATACCGACGGCGTATCGGGGGTCCCCGATGCCATCGTCTTGCCTG
TCGTCCTCATGCGTAGGGAT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MVNDRRRFLVIGARALAGASITWSGLAAAATPAIPAPKEKRLTPPRQLNLTNKPWTGDFDGMFKHRTIRVLVPYSRTLYF
NDRGRERGVTADTVRDFERYINKTMAAQLAKRPLTVYMIPVTRDRLLPDLNAGLGDIAAGNLTVTDSRLKQADFIVGQTS
KPIRELVITGPKSPTLATLDDLAGKAVHVRPSSSYYESLTALNDRFRKAGKPQMHLVLLPDALEDEDALEMLNAGTLQIL
VVDDWKARIWAQILKRIKVRDDLAVREGSKIGWAIRKGSPKLNAVLVDYNDNFIKKQGIADYRLKQYMKVIKQISDNTNG
AEIKRFQDTLAFFEKYGKLYHFDPLMLAAQGFQESQLNQNARSHVGAIGVMQIMPATGRELNVGSIKITESNIHAGTKYL
DQLMTRYFSDANFSESDRPLFAFASYNAGPGNISKMRTEAKARGLNPDKWFNNVEIVTSERIGIETTTYVRNIYKYYAAY
KLIEDAQEERSRAMKKVAG

Sequences:

>Translated_499_residues
MVNDRRRFLVIGARALAGASITWSGLAAAATPAIPAPKEKRLTPPRQLNLTNKPWTGDFDGMFKHRTIRVLVPYSRTLYF
NDRGRERGVTADTVRDFERYINKTMAAQLAKRPLTVYMIPVTRDRLLPDLNAGLGDIAAGNLTVTDSRLKQADFIVGQTS
KPIRELVITGPKSPTLATLDDLAGKAVHVRPSSSYYESLTALNDRFRKAGKPQMHLVLLPDALEDEDALEMLNAGTLQIL
VVDDWKARIWAQILKRIKVRDDLAVREGSKIGWAIRKGSPKLNAVLVDYNDNFIKKQGIADYRLKQYMKVIKQISDNTNG
AEIKRFQDTLAFFEKYGKLYHFDPLMLAAQGFQESQLNQNARSHVGAIGVMQIMPATGRELNVGSIKITESNIHAGTKYL
DQLMTRYFSDANFSESDRPLFAFASYNAGPGNISKMRTEAKARGLNPDKWFNNVEIVTSERIGIETTTYVRNIYKYYAAY
KLIEDAQEERSRAMKKVAG
>Mature_499_residues
MVNDRRRFLVIGARALAGASITWSGLAAAATPAIPAPKEKRLTPPRQLNLTNKPWTGDFDGMFKHRTIRVLVPYSRTLYF
NDRGRERGVTADTVRDFERYINKTMAAQLAKRPLTVYMIPVTRDRLLPDLNAGLGDIAAGNLTVTDSRLKQADFIVGQTS
KPIRELVITGPKSPTLATLDDLAGKAVHVRPSSSYYESLTALNDRFRKAGKPQMHLVLLPDALEDEDALEMLNAGTLQIL
VVDDWKARIWAQILKRIKVRDDLAVREGSKIGWAIRKGSPKLNAVLVDYNDNFIKKQGIADYRLKQYMKVIKQISDNTNG
AEIKRFQDTLAFFEKYGKLYHFDPLMLAAQGFQESQLNQNARSHVGAIGVMQIMPATGRELNVGSIKITESNIHAGTKYL
DQLMTRYFSDANFSESDRPLFAFASYNAGPGNISKMRTEAKARGLNPDKWFNNVEIVTSERIGIETTTYVRNIYKYYAAY
KLIEDAQEERSRAMKKVAG

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=466, Percent_Identity=21.4592274678112, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 56087; Mature: 56087

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNDRRRFLVIGARALAGASITWSGLAAAATPAIPAPKEKRLTPPRQLNLTNKPWTGDFD
CCCCCEEEEEEECHHHCCCCEEECCHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
GMFKHRTIRVLVPYSRTLYFNDRGRERGVTADTVRDFERYINKTMAAQLAKRPLTVYMIP
CCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
VTRDRLLPDLNAGLGDIAAGNLTVTDSRLKQADFIVGQTSKPIRELVITGPKSPTLATLD
CCHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHEEECCCCCCHHHHEEECCCCCCEEEHH
DLAGKAVHVRPSSSYYESLTALNDRFRKAGKPQMHLVLLPDALEDEDALEMLNAGTLQIL
HHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEE
VVDDWKARIWAQILKRIKVRDDLAVREGSKIGWAIRKGSPKLNAVLVDYNDNFIKKQGIA
EECCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCEEEEEEECCCCCHHHCCCH
DYRLKQYMKVIKQISDNTNGAEIKRFQDTLAFFEKYGKLYHFDPLMLAAQGFQESQLNQN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHCCCCHHHHHHH
ARSHVGAIGVMQIMPATGRELNVGSIKITESNIHAGTKYLDQLMTRYFSDANFSESDRPL
HHHHHCHHHHEEECCCCCCEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCE
FAFASYNAGPGNISKMRTEAKARGLNPDKWFNNVEIVTSERIGIETTTYVRNIYKYYAAY
EEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
KLIEDAQEERSRAMKKVAG
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVNDRRRFLVIGARALAGASITWSGLAAAATPAIPAPKEKRLTPPRQLNLTNKPWTGDFD
CCCCCEEEEEEECHHHCCCCEEECCHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
GMFKHRTIRVLVPYSRTLYFNDRGRERGVTADTVRDFERYINKTMAAQLAKRPLTVYMIP
CCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
VTRDRLLPDLNAGLGDIAAGNLTVTDSRLKQADFIVGQTSKPIRELVITGPKSPTLATLD
CCHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHEEECCCCCCHHHHEEECCCCCCEEEHH
DLAGKAVHVRPSSSYYESLTALNDRFRKAGKPQMHLVLLPDALEDEDALEMLNAGTLQIL
HHCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEE
VVDDWKARIWAQILKRIKVRDDLAVREGSKIGWAIRKGSPKLNAVLVDYNDNFIKKQGIA
EECCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCEEEEEEECCCCCHHHCCCH
DYRLKQYMKVIKQISDNTNGAEIKRFQDTLAFFEKYGKLYHFDPLMLAAQGFQESQLNQN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHCCCCHHHHHHH
ARSHVGAIGVMQIMPATGRELNVGSIKITESNIHAGTKYLDQLMTRYFSDANFSESDRPL
HHHHHCHHHHEEECCCCCCEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCE
FAFASYNAGPGNISKMRTEAKARGLNPDKWFNNVEIVTSERIGIETTTYVRNIYKYYAAY
EEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
KLIEDAQEERSRAMKKVAG
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA