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The map label for this gene is mutM

Identifier: 94309238

GI number: 94309238

Start: 309517

End: 310410

Strand: Direct

Name: mutM

Synonym: Rmet_0293

Alternate gene names: 94309238

Gene position: 309517-310410 (Clockwise)

Preceding gene: 94309231

Following gene: 94309239

Centisome position: 7.88

GC content: 69.13

Gene sequence:

>894_bases
ATGCCAGAACTGCCGGAAGTCGAAGTTACGCGGCGCGGGTTGCTGCCACACGTAGTGGGCCGCCGCATTGCCGACGTGAT
CGTGCGCCACCGGGGCTTGCGCTGGCCGGTCGAGCCGGAGTTGGAGGCGCGACTGACGGGCCGCATCATCGGCCGTATCG
AGCGACGCGGCAAGTATCTGCTGCTGGAATGCCTGCCGCCGGCCGACGCGACCCGGGCGGGGACAGGCGAGGACGCCGCG
CCGGGCTGGCTGCTGGTGCACCTGGGCATGACGGGCACGCTGCGCGTCTATCCCGCTCCGCCCGCGCCGGGCGCGCACGA
TCATCTCGACCTGTTGCTGGCTGCCGGCCCGGATACCGCGGAGGCAGAGCCGGTTGTCCTGCGTTTCCGCGACCCGCGCC
GCTTCGGCGCCATTCTCTGGACTCCGCTTGCCGAATCGGACCTGCCCGGGCATCCGCTGCTGAGCAGGCTCGGCATCGAG
CCCTTCGACCCGCGCTTCGACGGCGCCTGGCTACATCGTGGCATGCGCGGGCGCTCCATGGCGATCAAGCAGGCTCTGCT
GGCGGGCGACGTGGTCGTCGGCGTTGGCAACATCTACTGCTCCGAGAGCCTGTTTCGCGCGGGCATTCGGCCGACCACGC
AGGCCGGGCGGCTGAGCCTGGCGCGTTGCGAGAAGCTGGCCGTGGCCGTGCGCGAGACGCTGGCGGAGGCCATCGCACGC
GGCGGCAGCACATTGCGCGACTTCGTCGGCAGCGACGGCAGTAGCGGCTATTTCCAGCTCGACTGTTTTGTCTACGATCG
TGCCGGCGAGCCATGTCGCATTTGCGGCACTCCGATCCGTCAGATCCTGCAAGGCCAGCGATCGACGTTCTATTGCCCGC
ACTGCCAGCATTGA

Upstream 100 bases:

>100_bases
TCAGAAAAACAGGCAAGAGGTTCTTGGGAACTGATTAGATAAGGGCTGACAAGGGTCCGGAAGCCTGCTGCCGGCCGGCG
GATGGACTAAAGGGGGGGCG

Downstream 100 bases:

>100_bases
TCCGTCACCGGGGCGCAAAGCCTGTCCGGGACGGTCTGGCACGCGCCGAACCGGTCTGGATTTCTTGTTGAAATCGCCTG
CCGATGGGTCGGCGCAACAC

Product: formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPELPEVEVTRRGLLPHVVGRRIADVIVRHRGLRWPVEPELEARLTGRIIGRIERRGKYLLLECLPPADATRAGTGEDAA
PGWLLVHLGMTGTLRVYPAPPAPGAHDHLDLLLAAGPDTAEAEPVVLRFRDPRRFGAILWTPLAESDLPGHPLLSRLGIE
PFDPRFDGAWLHRGMRGRSMAIKQALLAGDVVVGVGNIYCSESLFRAGIRPTTQAGRLSLARCEKLAVAVRETLAEAIAR
GGSTLRDFVGSDGSSGYFQLDCFVYDRAGEPCRICGTPIRQILQGQRSTFYCPHCQH

Sequences:

>Translated_297_residues
MPELPEVEVTRRGLLPHVVGRRIADVIVRHRGLRWPVEPELEARLTGRIIGRIERRGKYLLLECLPPADATRAGTGEDAA
PGWLLVHLGMTGTLRVYPAPPAPGAHDHLDLLLAAGPDTAEAEPVVLRFRDPRRFGAILWTPLAESDLPGHPLLSRLGIE
PFDPRFDGAWLHRGMRGRSMAIKQALLAGDVVVGVGNIYCSESLFRAGIRPTTQAGRLSLARCEKLAVAVRETLAEAIAR
GGSTLRDFVGSDGSSGYFQLDCFVYDRAGEPCRICGTPIRQILQGQRSTFYCPHCQH
>Mature_296_residues
PELPEVEVTRRGLLPHVVGRRIADVIVRHRGLRWPVEPELEARLTGRIIGRIERRGKYLLLECLPPADATRAGTGEDAAP
GWLLVHLGMTGTLRVYPAPPAPGAHDHLDLLLAAGPDTAEAEPVVLRFRDPRRFGAILWTPLAESDLPGHPLLSRLGIEP
FDPRFDGAWLHRGMRGRSMAIKQALLAGDVVVGVGNIYCSESLFRAGIRPTTQAGRLSLARCEKLAVAVRETLAEAIARG
GSTLRDFVGSDGSSGYFQLDCFVYDRAGEPCRICGTPIRQILQGQRSTFYCPHCQH

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=296, Percent_Identity=48.3108108108108, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_RALME (Q1LRP7)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582448.1
- ProteinModelPortal:   Q1LRP7
- SMR:   Q1LRP7
- STRING:   Q1LRP7
- GeneID:   4037080
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0293
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- PhylomeDB:   Q1LRP7
- ProtClustDB:   PRK01103
- BioCyc:   RMET266264:RMET_0293-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 32540; Mature: 32409

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 287-287 BINDING 106-106 BINDING 133-133 BINDING 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVTRRGLLPHVVGRRIADVIVRHRGLRWPVEPELEARLTGRIIGRIERRGKYL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LLECLPPADATRAGTGEDAAPGWLLVHLGMTGTLRVYPAPPAPGAHDHLDLLLAAGPDTA
EEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCC
EAEPVVLRFRDPRRFGAILWTPLAESDLPGHPLLSRLGIEPFDPRFDGAWLHRGMRGRSM
CCCEEEEEECCCCHHCCEEECCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHH
AIKQALLAGDVVVGVGNIYCSESLFRAGIRPTTQAGRLSLARCEKLAVAVRETLAEAIAR
HHHHHHHHCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GGSTLRDFVGSDGSSGYFQLDCFVYDRAGEPCRICGTPIRQILQGQRSTFYCPHCQH
CCHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEECCHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
PELPEVEVTRRGLLPHVVGRRIADVIVRHRGLRWPVEPELEARLTGRIIGRIERRGKYL
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LLECLPPADATRAGTGEDAAPGWLLVHLGMTGTLRVYPAPPAPGAHDHLDLLLAAGPDTA
EEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCCC
EAEPVVLRFRDPRRFGAILWTPLAESDLPGHPLLSRLGIEPFDPRFDGAWLHRGMRGRSM
CCCEEEEEECCCCHHCCEEECCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHH
AIKQALLAGDVVVGVGNIYCSESLFRAGIRPTTQAGRLSLARCEKLAVAVRETLAEAIAR
HHHHHHHHCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GGSTLRDFVGSDGSSGYFQLDCFVYDRAGEPCRICGTPIRQILQGQRSTFYCPHCQH
CCHHHHHHHCCCCCCCEEEEEEEEEECCCCCCEECCHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA