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The map label for this gene is pth

Identifier: 94309232

GI number: 94309232

Start: 303018

End: 303614

Strand: Reverse

Name: pth

Synonym: Rmet_0287

Alternate gene names: 94309232

Gene position: 303614-303018 (Counterclockwise)

Preceding gene: 94309233

Following gene: 94309226

Centisome position: 7.73

GC content: 66.0

Gene sequence:

>597_bases
ATGATCAAGCTCATCGTCGGCCTCGGCAATCCCGGGGCGGAGTACGAAGCCACCCGGCACAACGCCGGCTTCTGGCTTGT
CGACCAACTCGCCCGCATGGGTGGCACAACCCTGCGCGTGGAAAGCCGCTTTCACGGCCTGGCTGGCCGCGCCCGTTTGT
GGGATCAGGATGTCTGGCTGCTCAAGCCTTCCACGTTCATGAACCGCTCCGGCCTGTCCGTGGTGTCGCTGGCGCGCTTT
TACAAGATCCTGCCCGACGAAATCGTGGTCGCCCACGACGAAATGGATCTGCCCGCCGGCTCGGCCAAGCTCAAGCGCGG
CGGTGGCTCGGGTGGCCACAATGGCCTCAAGGACATTTCGGCGCACCTGACCACTCAGGACTATTGGCGTCTGCGCATCG
GCGTCGGCCATCCGCGTAACGCGCCGGGCGGCGCGGCCGGTGGCCGCGAAGACGTGGTCAACTTCGTGCTCAAGCCACCA
CGCAAGGAAGAGCAGCAAGCCATCGACGAAGCGATCGACCGCTGCATCGGCCCGCTGGGCACGCTTGCGCGCGGCGAGAG
CGAGCGCGCGATGATGGAACTGCACACGGGCCGATAG

Upstream 100 bases:

>100_bases
TTTTTTTTCGCGCGGCTTTTTCGTACGCTTTTTCGCGTATCCTGTGTCGCGTTCCACGGTGCCCTCAGGCGTCCGATTTA
CCGCATTCCGTTCTCAACGC

Downstream 100 bases:

>100_bases
CACGCGCCCCGACAGCCGGCTCCAAAGAAAAAACCCCGCAAACCTGACGGTTTCGCGGGGTTTCTCTTTTCTGCTCTTGC
CTGTGGGGGCCGGAACGATA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVESRFHGLAGRARLWDQDVWLLKPSTFMNRSGLSVVSLARF
YKILPDEIVVAHDEMDLPAGSAKLKRGGGSGGHNGLKDISAHLTTQDYWRLRIGVGHPRNAPGGAAGGREDVVNFVLKPP
RKEEQQAIDEAIDRCIGPLGTLARGESERAMMELHTGR

Sequences:

>Translated_198_residues
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVESRFHGLAGRARLWDQDVWLLKPSTFMNRSGLSVVSLARF
YKILPDEIVVAHDEMDLPAGSAKLKRGGGSGGHNGLKDISAHLTTQDYWRLRIGVGHPRNAPGGAAGGREDVVNFVLKPP
RKEEQQAIDEAIDRCIGPLGTLARGESERAMMELHTGR
>Mature_198_residues
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVESRFHGLAGRARLWDQDVWLLKPSTFMNRSGLSVVSLARF
YKILPDEIVVAHDEMDLPAGSAKLKRGGGSGGHNGLKDISAHLTTQDYWRLRIGVGHPRNAPGGAAGGREDVVNFVLKPP
RKEEQQAIDEAIDRCIGPLGTLARGESERAMMELHTGR

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=173, Percent_Identity=34.1040462427746, Blast_Score=87, Evalue=7e-18,
Organism=Escherichia coli, GI1787455, Length=178, Percent_Identity=56.7415730337079, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_RALME (Q1LRQ3)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_582442.1
- ProteinModelPortal:   Q1LRQ3
- SMR:   Q1LRQ3
- STRING:   Q1LRQ3
- GeneID:   4037073
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_0287
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q1LRQ3
- ProtClustDB:   PRK05426
- BioCyc:   RMET266264:RMET_0287-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21630; Mature: 21630

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVESRFHGLAGRARLWDQDVWL
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHCCCCCCCEECCCCEEE
LKPSTFMNRSGLSVVSLARFYKILPDEIVVAHDEMDLPAGSAKLKRGGGSGGHNGLKDIS
ECCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCHHHHH
AHLTTQDYWRLRIGVGHPRNAPGGAAGGREDVVNFVLKPPRKEEQQAIDEAIDRCIGPLG
HHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHH
TLARGESERAMMELHTGR
HHHCCCCHHHHHEECCCC
>Mature Secondary Structure
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVESRFHGLAGRARLWDQDVWL
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHCCCCCCCEECCCCEEE
LKPSTFMNRSGLSVVSLARFYKILPDEIVVAHDEMDLPAGSAKLKRGGGSGGHNGLKDIS
ECCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCHHHHH
AHLTTQDYWRLRIGVGHPRNAPGGAAGGREDVVNFVLKPPRKEEQQAIDEAIDRCIGPLG
HHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHH
TLARGESERAMMELHTGR
HHHCCCCHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA