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The map label for this gene is rpoH [H]

Identifier: 94309217

GI number: 94309217

Start: 287945

End: 288880

Strand: Reverse

Name: rpoH [H]

Synonym: Rmet_0272

Alternate gene names: 94309217

Gene position: 288880-287945 (Counterclockwise)

Preceding gene: 94309219

Following gene: 94309211

Centisome position: 7.35

GC content: 63.78

Gene sequence:

>936_bases
GTGAACGCAGTCCTGTCTGCCGATCAAACCCAAGTTACCCGCCGTCTGCCCACGGCGCCCCAGAATGCCGCCGCGAATGC
CTTCGCACTGACCTTCCCCGGCACACTGGGTAATCTGGACAGCTACATTCAGGCTGTCCACCGCATCCCGATGCTCACCC
CCGAGGAAGAGCTCAGCCTGGCTCGCGACCTGCGCGACAACGACTCAGTCGATGCCGCCCGCCGCATGGTGATGTCGCAC
CTGCGTCTGGTGGTCTCCATTGCTCGCCAGTACCTGGGATACGGCTTGCCTCACGCCGACCTGATCCAGGAAGGCAACAT
CGGGCTGATGAAGGCCGTCAAGCGCTTCGACCCCGAACAGGGCGTGCGCCTGGTGTCGTACGCGATGCACTGGATCAAGG
CCGAGATCCACGAATACGTGTTGAAGAACTGGCGCATGGTCAAGGTGGCCACCACCAAGGCCCAGCGCAAGCTGTTCTTC
AACCTGCGCAGCCACAAGCAGGGCTCGCATACCTTCACGCCCGAGCAGATCGAAGCCGTTGCGCGCGAGCTCAACGTCAA
GCCCGAGGAAGTGATGGAGATGGAAACCCGCATGTCGGGCGGCGATATGGCGCTGGAAGGCCAGATCGAGGACGGCGAGG
AAGAGTTCGCGCCGATCGCCTACCTTGCGGATAACCACAACGAGCCGACGCAGGTGCTGGAAGCCAAGCGCCACGACCGT
CTGCAGGTCGAGGGCCTGGAAGAGGCGCTGACGAAGCTGGACCCGCGCAGCCGCCGCATCATCGAGGCGCGCTGGCTCGA
CGTCAACGACGATGGCTCGGGCGGCGCCACGCTGCACGAACTGGCCGACGAGTTCGGTGTATCCGCCGAGCGGATTCGCC
AGATCGAATCGGCGGCAATGAAGAAGATGAAGGGCGCGCTGGCGGCATTCGCCTGA

Upstream 100 bases:

>100_bases
TAACTTAGCACTCGTCCGGTTCGAGTGCTAATATGCGTTGCAATGCGGTGTCCCTTCGGGACACACCGGCAACCCACCGC
CGGATGCGAAAGGAGTCATA

Downstream 100 bases:

>100_bases
GGCCACGCACCCCGCAATGAGAAAGGCCGGTCATTAGACCGGCCTTTTCTTATTTACGACATCAACTGCTTGAGGTCATG
CGCGATCGGCTCCGGCCCCT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNAVLSADQTQVTRRLPTAPQNAAANAFALTFPGTLGNLDSYIQAVHRIPMLTPEEELSLARDLRDNDSVDAARRMVMSH
LRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPEQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFF
NLRSHKQGSHTFTPEQIEAVARELNVKPEEVMEMETRMSGGDMALEGQIEDGEEEFAPIAYLADNHNEPTQVLEAKRHDR
LQVEGLEEALTKLDPRSRRIIEARWLDVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALAAFA

Sequences:

>Translated_311_residues
MNAVLSADQTQVTRRLPTAPQNAAANAFALTFPGTLGNLDSYIQAVHRIPMLTPEEELSLARDLRDNDSVDAARRMVMSH
LRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPEQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFF
NLRSHKQGSHTFTPEQIEAVARELNVKPEEVMEMETRMSGGDMALEGQIEDGEEEFAPIAYLADNHNEPTQVLEAKRHDR
LQVEGLEEALTKLDPRSRRIIEARWLDVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALAAFA
>Mature_311_residues
MNAVLSADQTQVTRRLPTAPQNAAANAFALTFPGTLGNLDSYIQAVHRIPMLTPEEELSLARDLRDNDSVDAARRMVMSH
LRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPEQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFF
NLRSHKQGSHTFTPEQIEAVARELNVKPEEVMEMETRMSGGDMALEGQIEDGEEEFAPIAYLADNHNEPTQVLEAKRHDR
LQVEGLEEALTKLDPRSRRIIEARWLDVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALAAFA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=54.3478260869565, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1789098, Length=271, Percent_Identity=32.1033210332103, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=30.8641975308642, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34896; Mature: 34896

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVLSADQTQVTRRLPTAPQNAAANAFALTFPGTLGNLDSYIQAVHRIPMLTPEEELSL
CCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ARDLRDNDSVDAARRMVMSHLRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPEQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCHHH
GVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNLRSHKQGSHTFTPEQIEAV
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ARELNVKPEEVMEMETRMSGGDMALEGQIEDGEEEFAPIAYLADNHNEPTQVLEAKRHDR
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCEEEEECCCCCHHHHHHHHHCCC
LQVEGLEEALTKLDPRSRRIIEARWLDVNDDGSGGATLHELADEFGVSAERIRQIESAAM
CHHHHHHHHHHHCCCCHHHEEHEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
KKMKGALAAFA
HHHHHHHHHCC
>Mature Secondary Structure
MNAVLSADQTQVTRRLPTAPQNAAANAFALTFPGTLGNLDSYIQAVHRIPMLTPEEELSL
CCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHH
ARDLRDNDSVDAARRMVMSHLRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPEQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCHHH
GVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNLRSHKQGSHTFTPEQIEAV
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ARELNVKPEEVMEMETRMSGGDMALEGQIEDGEEEFAPIAYLADNHNEPTQVLEAKRHDR
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCEEEEECCCCCHHHHHHHHHCCC
LQVEGLEEALTKLDPRSRRIIEARWLDVNDDGSGGATLHELADEFGVSAERIRQIESAAM
CHHHHHHHHHHHCCCCHHHEEHEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
KKMKGALAAFA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]