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The map label for this gene is ptsI [H]

Identifier: 94309191

GI number: 94309191

Start: 262011

End: 263768

Strand: Direct

Name: ptsI [H]

Synonym: Rmet_0246

Alternate gene names: 94309191

Gene position: 262011-263768 (Clockwise)

Preceding gene: 94309190

Following gene: 94309192

Centisome position: 6.67

GC content: 65.93

Gene sequence:

>1758_bases
ATGCCCTTTGCCTTGCACGGCATTCCGGTTTCTCGTGGGGTCGCCATCGGGCGAGCCCATATTCTTGCGCCGGCTGCGCT
GGATGTGTCGCACTACCTGGTCGATGAAGACCGGCTCGAAGCCGAGGTGGAGCGATTGCGTTCGGCACGCGCCGCGGTGC
GGGCCGAGCTGATCACGCTCAAGCGTGACCTGCCGCGCGACGCGCCGGAGGAAATGGGGGCATTTCTCGATGTCCACGCG
ATGATCCTCGACGACGAGGCCCTGTCGCGCGAACCCGAGACGCTGATTCTCCAACGCCGCTATAACGCCGAGTGGGCCCT
GACCACGCGACTCGAAGAACTGATGCGTCAGTTCGACGAGATCGAGGACGAGTATCTGCGCGAGCGCAAGGCCGACATCG
AGCAGGTGGTGGAGCGGATCCTGAAGGTGCTGGCCGGAGCGCCCGTGCTGGCGCCGGCGCCCGTGCCGACGCTCGCGCCG
GATGGCGAGGCCACGCCGGGGGTGATCGTGGTGGCCCACGACATCGGCCCGGCCGACATGCTCCAGTTCAAGCACACCGT
GTTCCACGGTTTCGTGACGGACCTGGGCGGCCGTACATCGCACACGGCTATCGTCGCGCGCAGTCTCGACATCCCCGCCG
CGGTGGGCGTCAAGAACGCGAGCGAACTGATCCGTCAGGACGACTGGATCATCATCGATGGCGATGCCGGCCTGGTGATC
GTCGATCCGTCGGCCATCATTCTCGAAGAGTATCGGCACCGCCAGAGCGAGCGCGCGCTCGAGAAGAAGCGCCTGCAGCG
GCTGCGCCACATGCCGGCGGTGACGCTGGACGGGCTCGAGATCGAACTGCTGGCCAATATCGAGATGGCGGAGGATGCCG
GTGCGGCGCTGTCCGCCGGCGCCGTCGGGGTGGGGCTGTTCCGGTCCGAATTCCTGTTCATGAACCGGCGCGACGAGCTG
CCGGATGAAGACGAGCAATTTCACGCGTATCGCAACGCCGTGGAAGCGATGCACGGGCTGCCGGTCACGATTCGGACCAT
CGACATCGGCGCCGACAAGCCGCTCGACGGCCGTGGCGACGACTTCGAGACCGCGCTGAACCCCGCACTGGGTTTGCGCG
CGATCCGCTGGTCGTTGTCAGAGCCGGGGATGTTCCTGACCCAGTTGCGGGCGTTGCTGCGCGCTTCCGCGTTCGGCCCG
GTCCGGATTCTGATCCCGATGCTGGCTCACGCCGGTGAGATCGACCAGACGCTGGACCTGATCGAGCAAGCCAAGCGCCA
GCTCGACGAACGCGGCCTGCCGTACGACCCGGGTATCAAGGTCGGGGCGATGATTGAGATCCCGGCTGCCGTGCTGCTGC
TGCCGCTGTTCCTCAAGCGCATGGACTTCCTGTCCATCGGCACCAATGACCTGATCCAGTACACGCTGGCGATCGACCGC
GCCGACAATGCCGTGGCGCACCTCTACGACCCGCTTCACCCGGCCGTGCTGCAGCTGATTGCCCGGACGATCCGCGAAGC
CAACCATGCGGGCGTGCCCGTCGCCGTGTGTGGCGAGATGGCGGGGGATCCGGCGATGACCCGGCTGCTGCTGGGCATGG
GACTACGCGAGTTCTCGATGCACCCGGCGCAATTGCTGCGGGTGAAGCAGGAAGTCATCCATGCGGACTGCGAGCGCCTG
AGCAAGGCGGTCGACCAGATCCTGAACTGCTATGAACCGGACGAGCTTACGGCCGCGTTGAAGCAACTGACAGCGTGA

Upstream 100 bases:

>100_bases
TGGATGCGCTGCTGGCGCTGATCGCCAACCGCTTCGGCGAAGGCGAGTGAAGTTCGACGGTAATCCAGGCTTTTTCTAAA
CGTTCCTGACGGAGCGGTTC

Downstream 100 bases:

>100_bases
CGCCACCCTGAAACCCTGAAGATTTCAGGGTTTTTAGGGTGCGAATGTGGCGTCGTTTGCGGCACTCGCGTGTCTGGAAG
TGGCTTTTTAATCGCTTAAA

Product: phosphoenolpyruvate-protein phosphotransferase of PTS system (enzyme I)

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MPFALHGIPVSRGVAIGRAHILAPAALDVSHYLVDEDRLEAEVERLRSARAAVRAELITLKRDLPRDAPEEMGAFLDVHA
MILDDEALSREPETLILQRRYNAEWALTTRLEELMRQFDEIEDEYLRERKADIEQVVERILKVLAGAPVLAPAPVPTLAP
DGEATPGVIVVAHDIGPADMLQFKHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVKNASELIRQDDWIIIDGDAGLVI
VDPSAIILEEYRHRQSERALEKKRLQRLRHMPAVTLDGLEIELLANIEMAEDAGAALSAGAVGVGLFRSEFLFMNRRDEL
PDEDEQFHAYRNAVEAMHGLPVTIRTIDIGADKPLDGRGDDFETALNPALGLRAIRWSLSEPGMFLTQLRALLRASAFGP
VRILIPMLAHAGEIDQTLDLIEQAKRQLDERGLPYDPGIKVGAMIEIPAAVLLLPLFLKRMDFLSIGTNDLIQYTLAIDR
ADNAVAHLYDPLHPAVLQLIARTIREANHAGVPVAVCGEMAGDPAMTRLLLGMGLREFSMHPAQLLRVKQEVIHADCERL
SKAVDQILNCYEPDELTAALKQLTA

Sequences:

>Translated_585_residues
MPFALHGIPVSRGVAIGRAHILAPAALDVSHYLVDEDRLEAEVERLRSARAAVRAELITLKRDLPRDAPEEMGAFLDVHA
MILDDEALSREPETLILQRRYNAEWALTTRLEELMRQFDEIEDEYLRERKADIEQVVERILKVLAGAPVLAPAPVPTLAP
DGEATPGVIVVAHDIGPADMLQFKHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVKNASELIRQDDWIIIDGDAGLVI
VDPSAIILEEYRHRQSERALEKKRLQRLRHMPAVTLDGLEIELLANIEMAEDAGAALSAGAVGVGLFRSEFLFMNRRDEL
PDEDEQFHAYRNAVEAMHGLPVTIRTIDIGADKPLDGRGDDFETALNPALGLRAIRWSLSEPGMFLTQLRALLRASAFGP
VRILIPMLAHAGEIDQTLDLIEQAKRQLDERGLPYDPGIKVGAMIEIPAAVLLLPLFLKRMDFLSIGTNDLIQYTLAIDR
ADNAVAHLYDPLHPAVLQLIARTIREANHAGVPVAVCGEMAGDPAMTRLLLGMGLREFSMHPAQLLRVKQEVIHADCERL
SKAVDQILNCYEPDELTAALKQLTA
>Mature_584_residues
PFALHGIPVSRGVAIGRAHILAPAALDVSHYLVDEDRLEAEVERLRSARAAVRAELITLKRDLPRDAPEEMGAFLDVHAM
ILDDEALSREPETLILQRRYNAEWALTTRLEELMRQFDEIEDEYLRERKADIEQVVERILKVLAGAPVLAPAPVPTLAPD
GEATPGVIVVAHDIGPADMLQFKHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVKNASELIRQDDWIIIDGDAGLVIV
DPSAIILEEYRHRQSERALEKKRLQRLRHMPAVTLDGLEIELLANIEMAEDAGAALSAGAVGVGLFRSEFLFMNRRDELP
DEDEQFHAYRNAVEAMHGLPVTIRTIDIGADKPLDGRGDDFETALNPALGLRAIRWSLSEPGMFLTQLRALLRASAFGPV
RILIPMLAHAGEIDQTLDLIEQAKRQLDERGLPYDPGIKVGAMIEIPAAVLLLPLFLKRMDFLSIGTNDLIQYTLAIDRA
DNAVAHLYDPLHPAVLQLIARTIREANHAGVPVAVCGEMAGDPAMTRLLLGMGLREFSMHPAQLLRVKQEVIHADCERLS
KAVDQILNCYEPDELTAALKQLTA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=579, Percent_Identity=38.860103626943, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789193, Length=563, Percent_Identity=33.5701598579041, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI48994992, Length=519, Percent_Identity=32.9479768786127, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1788726, Length=589, Percent_Identity=30.7300509337861, Blast_Score=242, Evalue=6e-65,
Organism=Escherichia coli, GI1787994, Length=400, Percent_Identity=27.5, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI226510935, Length=173, Percent_Identity=28.3236994219653, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64538; Mature: 64407

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFALHGIPVSRGVAIGRAHILAPAALDVSHYLVDEDRLEAEVERLRSARAAVRAELITL
CCEEECCCCHHCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KRDLPRDAPEEMGAFLDVHAMILDDEALSREPETLILQRRYNAEWALTTRLEELMRQFDE
HHHCCCCCHHHHHHHHHHHHHEECCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
IEDEYLRERKADIEQVVERILKVLAGAPVLAPAPVPTLAPDGEATPGVIVVAHDIGPADM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
LQFKHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVKNASELIRQDDWIIIDGDAGLVI
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCHHHHHHCCCEEEEECCCCEEE
VDPSAIILEEYRHRQSERALEKKRLQRLRHMPAVTLDGLEIELLANIEMAEDAGAALSAG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCHHHCCCCHHHCC
AVGVGLFRSEFLFMNRRDELPDEDEQFHAYRNAVEAMHGLPVTIRTIDIGADKPLDGRGD
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
DFETALNPALGLRAIRWSLSEPGMFLTQLRALLRASAFGPVRILIPMLAHAGEIDQTLDL
CHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHH
IEQAKRQLDERGLPYDPGIKVGAMIEIPAAVLLLPLFLKRMDFLSIGTNDLIQYTLAIDR
HHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHEEEHHEEEC
ADNAVAHLYDPLHPAVLQLIARTIREANHAGVPVAVCGEMAGDPAMTRLLLGMGLREFSM
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHCCHHHHCC
HPAQLLRVKQEVIHADCERLSKAVDQILNCYEPDELTAALKQLTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PFALHGIPVSRGVAIGRAHILAPAALDVSHYLVDEDRLEAEVERLRSARAAVRAELITL
CEEECCCCHHCCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KRDLPRDAPEEMGAFLDVHAMILDDEALSREPETLILQRRYNAEWALTTRLEELMRQFDE
HHHCCCCCHHHHHHHHHHHHHEECCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
IEDEYLRERKADIEQVVERILKVLAGAPVLAPAPVPTLAPDGEATPGVIVVAHDIGPADM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHH
LQFKHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVKNASELIRQDDWIIIDGDAGLVI
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCHHHHHHCCCEEEEECCCCEEE
VDPSAIILEEYRHRQSERALEKKRLQRLRHMPAVTLDGLEIELLANIEMAEDAGAALSAG
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCHHHCCCCHHHCC
AVGVGLFRSEFLFMNRRDELPDEDEQFHAYRNAVEAMHGLPVTIRTIDIGADKPLDGRGD
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
DFETALNPALGLRAIRWSLSEPGMFLTQLRALLRASAFGPVRILIPMLAHAGEIDQTLDL
CHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHH
IEQAKRQLDERGLPYDPGIKVGAMIEIPAAVLLLPLFLKRMDFLSIGTNDLIQYTLAIDR
HHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHEEEHHEEEC
ADNAVAHLYDPLHPAVLQLIARTIREANHAGVPVAVCGEMAGDPAMTRLLLGMGLREFSM
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHCCHHHHCC
HPAQLLRVKQEVIHADCERLSKAVDQILNCYEPDELTAALKQLTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]