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The map label for this gene is rhtB [H]

Identifier: 94309181

GI number: 94309181

Start: 252302

End: 252946

Strand: Direct

Name: rhtB [H]

Synonym: Rmet_0236

Alternate gene names: 94309181

Gene position: 252302-252946 (Clockwise)

Preceding gene: 94309180

Following gene: 94309184

Centisome position: 6.42

GC content: 63.41

Gene sequence:

>645_bases
ATGCGTTGGGAAGTCTGGCTGGCCTATTTTGCCGCGTGCTGGGTGATCGCTGTCTCGCCCGGTTCGGGCGCCGTGCTGTC
GATGAGCCACGGCCTGTCATACGGCCTGAAGAAGACGTCGACGACGATTCTGGGCCTGCAGACCGGCCTGGTGATCATCC
TGCTGATCGCAGGCGGTGGACTTGGCGCACTGCTGCTGGCGTCCGAAGAAGCATTCCTGGTGGTCAAGACGATTGGCGCG
ATGTATCTGATCTACCTGGGTATCCAGCAGTGGCGTGCCAAGGTTGAGACGAACCCGCAGAACAGCGAGGACAAGACGGT
GCGCGTGACCATCATGAGCCGGCGCCGGCGCTTCGCCACGGGGCTGCTGACGAACGTCACCAACCCCAAGGGCATCATCT
TCATGGTGGCCGTGCTGCCGCAGTTCATCGACCCGACGCATGCGCTCGGGCTGCAACTGGCTATCCTGGCGGCGACGATG
TGCTTTGTGGACCTGATCGTGATGCACGGCTATGCGCTACTGGCGTCGCGCATGCAGGGCCTGTTCCGCAACGCGCGTGC
CGTGCGCTGGCAAAACCGCTTCTTCGGCAGCGTGCTGGTGGCCGTGGGCACCGCGCTGTTCTTTGTCCGGCGTCATCACG
CCTGA

Upstream 100 bases:

>100_bases
GGATGCGCGCCAGGCCGAGCAGGTCGAGCAGTTCCTCACCGGTTTTCACCCGTTCTAAATCCGTTCCAAGTCTTTCAGGT
AGTCAATCAAGGAGTTCGTC

Downstream 100 bases:

>100_bases
GCGTTGATGGCGAGGGCGGGTGGCGCGCAGTCGACAGGTTTGCTTCAATAGGGAGTGATCAGGAATCTATGGAGAACCCC
GATGCCTGCCGTCGCCCACC

Product: neutral amino-acid efflux system

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGLKKTSTTILGLQTGLVIILLIAGGGLGALLLASEEAFLVVKTIGA
MYLIYLGIQQWRAKVETNPQNSEDKTVRVTIMSRRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPTHALGLQLAILAATM
CFVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLVAVGTALFFVRRHHA

Sequences:

>Translated_214_residues
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGLKKTSTTILGLQTGLVIILLIAGGGLGALLLASEEAFLVVKTIGA
MYLIYLGIQQWRAKVETNPQNSEDKTVRVTIMSRRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPTHALGLQLAILAATM
CFVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLVAVGTALFFVRRHHA
>Mature_214_residues
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGLKKTSTTILGLQTGLVIILLIAGGGLGALLLASEEAFLVVKTIGA
MYLIYLGIQQWRAKVETNPQNSEDKTVRVTIMSRRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPTHALGLQLAILAATM
CFVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLVAVGTALFFVRRHHA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=212, Percent_Identity=41.0377358490566, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1788099, Length=169, Percent_Identity=28.4023668639053, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23479; Mature: 23479

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGLKKTSTTILGLQTGLVIILLIAGGG
CCHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCHHHHHEEEEHHHHHHEEEEECCCC
LGALLLASEEAFLVVKTIGAMYLIYLGIQQWRAKVETNPQNSEDKTVRVTIMSRRRRFAT
CEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHH
GLLTNVTNPKGIIFMVAVLPQFIDPTHALGLQLAILAATMCFVDLIVMHGYALLASRMQG
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRNARAVRWQNRFFGSVLVAVGTALFFVRRHHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGLKKTSTTILGLQTGLVIILLIAGGG
CCHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCHHHHHEEEEHHHHHHEEEEECCCC
LGALLLASEEAFLVVKTIGAMYLIYLGIQQWRAKVETNPQNSEDKTVRVTIMSRRRRFAT
CEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHH
GLLTNVTNPKGIIFMVAVLPQFIDPTHALGLQLAILAATMCFVDLIVMHGYALLASRMQG
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRNARAVRWQNRFFGSVLVAVGTALFFVRRHHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]