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The map label for this gene is dac [H]

Identifier: 94309170

GI number: 94309170

Start: 238765

End: 239958

Strand: Reverse

Name: dac [H]

Synonym: Rmet_0225

Alternate gene names: 94309170

Gene position: 239958-238765 (Counterclockwise)

Preceding gene: 94309171

Following gene: 94309169

Centisome position: 6.11

GC content: 63.32

Gene sequence:

>1194_bases
ATGCTGAACAGAGTCACGCCGTACGTCGCATCCGCCATTGCTCCCGTAATCGTTGCGGCAACGCTCGCCGCCGCGCCCGC
AGCCGTGATGGCGCAAGGCGTGCCGGTGCCACAGGTCGCCGCCAAGTCCTGGATGCTGTACGACGTGACGAGCGGCCAGG
CGCTGGCGTCACAGAATGCAGATATGCGCATCGAGCCGGCGTCGCTGACCAAGCTGATGACGGCTTACCTGGCCTTCGAG
GCGCTCAAGGAAAAACGCCTGACGCTGGATCAGACCGTCACGCCGACCGACATCGTCCGCAAGGTGAAAAGCGATGAATC
GCGCATGTTCATCGAAGCCGGCAAGCCGGTCAGCGTGCAGGACCTTCTGCTGGGCCTGATCGTCCAGTCGGGCAACGACG
CCTCGCTGGCGCTGGCCGAAGCCGTGGGCGGCTCGGAAGAAGGCTTCGTCGTGATGATGAACCGTGAAGCCCAGCGCATG
GGCATGAAGAACACGCACTTCACGAACACCGACGGTGTGCCCGATCCGAACCACTACACCACGGCGGTGGACCTGGCGAT
CCTGACCACGCGCCTGATCAAGGACTTTCCCGAGTACTACTCGATGTACTCGCAGAAGGAGTTCACCTATAACAAGATCA
GGCAGCCGAACCGCAACCGTCTGCTGTACATCGACCCGACCGTGGACGGCGTGAAGACCGGCCATACGAAGTCCGCGGGC
TACTGCCTGATTTCGTCGGCAAAGCGTCCGCTGGCCAATGTGCCCAACGGCTCGCGCCGACTGGTGTCGATCGTGATCGG
CACGGCCACCGAGCAGATCCGCACACAGGAAAGCCTGAAGATCCTGAACTACGGCTTCCAGTTCTTCGACACGCTGCGCC
TGTACGACAAGGGCCAGGTGCTGGCCACGCCCGACATCTACAAGGGCAAGACCGGCACGATCAAGATCGGCGTGGCCAAC
GAGACGTTCGTCACGGTGCCCAAGGGCACGGGCTCGCGCCTCAAGCCGGTGCTGGAGCGCCAGGAACTGCTGATCGCGCC
GATCACGGCCGGCCAGCAGGTGGGCACCGTCAAGCTGATGGACGGCGCCAACAAGGTGGCGGAATTCCCGGTGGTGGCGC
TCGAGGAAGTGCCGGAGGCCGGCTTCTTCGGCCGCCTGTGGGATACGATCCGCCTCTGGTTCAAGCGCAAGTAA

Upstream 100 bases:

>100_bases
TTTCATCGCAAGCTGCACCTGATCAAGCAGCTCGTCGTCAACGCGTGGGACCGGTGAATCGCCCCGCCCGCGCCAATCCT
TTCTTGTTGGAAACCTACAC

Downstream 100 bases:

>100_bases
GAGTTCAAGGATTCGAGATGTCTACAGATTCGAACAACGGTCCGCTGATTCCGGGGGAGATTCCGCCGGAACAGTCGCTG
ATCGAGTACCCGAGCGATTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6; PBP-6 [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MLNRVTPYVASAIAPVIVAATLAAAPAAVMAQGVPVPQVAAKSWMLYDVTSGQALASQNADMRIEPASLTKLMTAYLAFE
ALKEKRLTLDQTVTPTDIVRKVKSDESRMFIEAGKPVSVQDLLLGLIVQSGNDASLALAEAVGGSEEGFVVMMNREAQRM
GMKNTHFTNTDGVPDPNHYTTAVDLAILTTRLIKDFPEYYSMYSQKEFTYNKIRQPNRNRLLYIDPTVDGVKTGHTKSAG
YCLISSAKRPLANVPNGSRRLVSIVIGTATEQIRTQESLKILNYGFQFFDTLRLYDKGQVLATPDIYKGKTGTIKIGVAN
ETFVTVPKGTGSRLKPVLERQELLIAPITAGQQVGTVKLMDGANKVAEFPVVALEEVPEAGFFGRLWDTIRLWFKRK

Sequences:

>Translated_397_residues
MLNRVTPYVASAIAPVIVAATLAAAPAAVMAQGVPVPQVAAKSWMLYDVTSGQALASQNADMRIEPASLTKLMTAYLAFE
ALKEKRLTLDQTVTPTDIVRKVKSDESRMFIEAGKPVSVQDLLLGLIVQSGNDASLALAEAVGGSEEGFVVMMNREAQRM
GMKNTHFTNTDGVPDPNHYTTAVDLAILTTRLIKDFPEYYSMYSQKEFTYNKIRQPNRNRLLYIDPTVDGVKTGHTKSAG
YCLISSAKRPLANVPNGSRRLVSIVIGTATEQIRTQESLKILNYGFQFFDTLRLYDKGQVLATPDIYKGKTGTIKIGVAN
ETFVTVPKGTGSRLKPVLERQELLIAPITAGQQVGTVKLMDGANKVAEFPVVALEEVPEAGFFGRLWDTIRLWFKRK
>Mature_397_residues
MLNRVTPYVASAIAPVIVAATLAAAPAAVMAQGVPVPQVAAKSWMLYDVTSGQALASQNADMRIEPASLTKLMTAYLAFE
ALKEKRLTLDQTVTPTDIVRKVKSDESRMFIEAGKPVSVQDLLLGLIVQSGNDASLALAEAVGGSEEGFVVMMNREAQRM
GMKNTHFTNTDGVPDPNHYTTAVDLAILTTRLIKDFPEYYSMYSQKEFTYNKIRQPNRNRLLYIDPTVDGVKTGHTKSAG
YCLISSAKRPLANVPNGSRRLVSIVIGTATEQIRTQESLKILNYGFQFFDTLRLYDKGQVLATPDIYKGKTGTIKIGVAN
ETFVTVPKGTGSRLKPVLERQELLIAPITAGQQVGTVKLMDGANKVAEFPVVALEEVPEAGFFGRLWDTIRLWFKRK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=381, Percent_Identity=41.2073490813648, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1786851, Length=368, Percent_Identity=39.945652173913, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082022, Length=387, Percent_Identity=40.3100775193798, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082054, Length=221, Percent_Identity=31.6742081447964, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43509; Mature: 43509

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRVTPYVASAIAPVIVAATLAAAPAAVMAQGVPVPQVAAKSWMLYDVTSGQALASQNA
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHCCEEEEEECCCCHHHCCCC
DMRIEPASLTKLMTAYLAFEALKEKRLTLDQTVTPTDIVRKVKSDESRMFIEAGKPVSVQ
CCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHH
DLLLGLIVQSGNDASLALAEAVGGSEEGFVVMMNREAQRMGMKNTHFTNTDGVPDPNHYT
HHHHHHHHCCCCCCCEEHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCH
TAVDLAILTTRLIKDFPEYYSMYSQKEFTYNKIRQPNRNRLLYIDPTVDGVKTGHTKSAG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCCC
YCLISSAKRPLANVPNGSRRLVSIVIGTATEQIRTQESLKILNYGFQFFDTLRLYDKGQV
EEEEECCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LATPDIYKGKTGTIKIGVANETFVTVPKGTGSRLKPVLERQELLIAPITAGQQVGTVKLM
EECCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEE
DGANKVAEFPVVALEEVPEAGFFGRLWDTIRLWFKRK
CCCCHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNRVTPYVASAIAPVIVAATLAAAPAAVMAQGVPVPQVAAKSWMLYDVTSGQALASQNA
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHCCEEEEEECCCCHHHCCCC
DMRIEPASLTKLMTAYLAFEALKEKRLTLDQTVTPTDIVRKVKSDESRMFIEAGKPVSVQ
CCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHH
DLLLGLIVQSGNDASLALAEAVGGSEEGFVVMMNREAQRMGMKNTHFTNTDGVPDPNHYT
HHHHHHHHCCCCCCCEEHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCH
TAVDLAILTTRLIKDFPEYYSMYSQKEFTYNKIRQPNRNRLLYIDPTVDGVKTGHTKSAG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCCCC
YCLISSAKRPLANVPNGSRRLVSIVIGTATEQIRTQESLKILNYGFQFFDTLRLYDKGQV
EEEEECCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LATPDIYKGKTGTIKIGVANETFVTVPKGTGSRLKPVLERQELLIAPITAGQQVGTVKLM
EECCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEE
DGANKVAEFPVVALEEVPEAGFFGRLWDTIRLWFKRK
CCCCHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3279397; 8905232; 9278503; 7042389 [H]