Click here to switch to the map view.

The map label for this gene is xthA1 [H]

Identifier: 94309094

GI number: 94309094

Start: 153809

End: 154585

Strand: Reverse

Name: xthA1 [H]

Synonym: Rmet_0149

Alternate gene names: 94309094

Gene position: 154585-153809 (Counterclockwise)

Preceding gene: 94309097

Following gene: 94309093

Centisome position: 3.94

GC content: 59.59

Gene sequence:

>777_bases
ATGTTACGGATTATCAGCGCCAACCTCAACGGCATCCGCTCCGCCTCCAAGAAAGGCTTCTTCGAGTGGATGGGCAAGCA
GGAAGCGGACATGGTCTGCGTTCAGGAACTCAAGGCCCAGGAAGCCGACATGACCGAGGCGTTCCTGGCGCCACACGGCT
ATCACGGCTACTTCCACTATGCCGAGAAAAAAGGCTACAGCGGCGTGGGCCTCTATACCCGCCACAAGCCGGAGCGCGTG
ATCATCGGCTTCGGCAATACGGAGTTCGATGCTGAGGGTCGCTACGTCGAAGTGCAGTATCCGCACCTCGCCGTGATCTC
GGTCTACGTGCCTTCCGGCTCAAGCTCGGAGGAGCGCCAGCAGGCCAAGTTCCGCTTTATGGAAGCCTTCCTGCCGCACC
TGCTGGAGCTGAAAGCCAGCGGCCGCGAGATCGTGCTGTGCGGTGACGTGAACATCGCCCACAAGGAAATCGACATCAAG
AACTGGAAGGGCAACCTGAAAAACTCCGGTTTCCTGCCCGAAGAGCGCGCCTGGATCGGCGAACTGTTCGACAACCACGG
CTATGTCGACGTATTCCGCCAGCTGGACCCGCGCCCCGAACAGTACACATGGTGGAGCAACCGCGGTCAGGCGTACGCGA
AGAACGTGGGGTGGCGCATCGATTACCACCTGACCACGGCGAAAATCGCGGCTACCGCCAAGGCTACGTCGATCTACAAG
GACGAGAAGTTCAGCGACCACGCCCCGCTGTCGATCGATTACGATTTTCCGTTGTGA

Upstream 100 bases:

>100_bases
GCACGCCCGCGTCCAGTGCAAAGCGGATGAAGGACTGGCTAAGGTCGTCCGGCGTCGTCTGCTGCGTCATCTTTTCTCTC
TGAATTCAAAATTTCCCGAG

Downstream 100 bases:

>100_bases
TGTGCGCATCCCCCGGCGCAACGCCGGGGGTGCCGTCCGCCTTCAGATGGCGCGCGTCTCATCGAACAGGTTCGGGTGAC
GGTCCAGCAGCTTCAGCAGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLRIISANLNGIRSASKKGFFEWMGKQEADMVCVQELKAQEADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERV
IIGFGNTEFDAEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLELKASGREIVLCGDVNIAHKEIDIK
NWKGNLKNSGFLPEERAWIGELFDNHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLTTAKIAATAKATSIYK
DEKFSDHAPLSIDYDFPL

Sequences:

>Translated_258_residues
MLRIISANLNGIRSASKKGFFEWMGKQEADMVCVQELKAQEADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERV
IIGFGNTEFDAEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLELKASGREIVLCGDVNIAHKEIDIK
NWKGNLKNSGFLPEERAWIGELFDNHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLTTAKIAATAKATSIYK
DEKFSDHAPLSIDYDFPL
>Mature_258_residues
MLRIISANLNGIRSASKKGFFEWMGKQEADMVCVQELKAQEADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERV
IIGFGNTEFDAEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLELKASGREIVLCGDVNIAHKEIDIK
NWKGNLKNSGFLPEERAWIGELFDNHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLTTAKIAATAKATSIYK
DEKFSDHAPLSIDYDFPL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=38.5826771653543, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=38.5826771653543, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=38.5826771653543, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375507, Length=310, Percent_Identity=27.0967741935484, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=30.3370786516854, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=31.6602316602317, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI221330655, Length=256, Percent_Identity=35.546875, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI17136678, Length=252, Percent_Identity=36.1111111111111, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29538; Mature: 29538

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIISANLNGIRSASKKGFFEWMGKQEADMVCVQELKAQEADMTEAFLAPHGYHGYFHY
CEEEEECCCCHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEE
AEKKGYSGVGLYTRHKPERVIIGFGNTEFDAEGRYVEVQYPHLAVISVYVPSGSSSEERQ
HHCCCCCCCCEEECCCCCEEEEECCCCEECCCCCEEEEECCCEEEEEEEECCCCCHHHHH
QAKFRFMEAFLPHLLELKASGREIVLCGDVNIAHKEIDIKNWKGNLKNSGFLPEERAWIG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHH
ELFDNHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLTTAKIAATAKATSIYK
HHHCCCCHHHHHHHCCCCCCCEEEECCCCCEEECCCCEEEEEEEEEHHHHHHHHHHHHCC
DEKFSDHAPLSIDYDFPL
CCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MLRIISANLNGIRSASKKGFFEWMGKQEADMVCVQELKAQEADMTEAFLAPHGYHGYFHY
CEEEEECCCCHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEE
AEKKGYSGVGLYTRHKPERVIIGFGNTEFDAEGRYVEVQYPHLAVISVYVPSGSSSEERQ
HHCCCCCCCCEEECCCCCEEEEECCCCEECCCCCEEEEECCCEEEEEEEECCCCCHHHHH
QAKFRFMEAFLPHLLELKASGREIVLCGDVNIAHKEIDIKNWKGNLKNSGFLPEERAWIG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHH
ELFDNHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLTTAKIAATAKATSIYK
HHHCCCCHHHHHHHCCCCCCCEEEECCCCCEEECCCCEEEEEEEEEHHHHHHHHHHHHCC
DEKFSDHAPLSIDYDFPL
CCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]