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The map label for this gene is pcaD [H]

Identifier: 94309029

GI number: 94309029

Start: 91161

End: 92009

Strand: Direct

Name: pcaD [H]

Synonym: Rmet_0084

Alternate gene names: 94309029

Gene position: 91161-92009 (Clockwise)

Preceding gene: 94309028

Following gene: 94309030

Centisome position: 2.32

GC content: 63.84

Gene sequence:

>849_bases
ATGTCTGACACTTCCGCATCGACCGCGTCGCAAACGAACCGGCTTCGGGTCGGGGATGTGCATTTGCAAGTGCGCGTCGA
TGGTACCGAAGGCCCTTGGGTGATCCTGGCCCATGCGCTCGGTGCGAATCTGACTTTGTGGGATGACACCGCGCGGCACC
TTGCCGCACGCTATCGCGTGCTGCGTTTCGACATGCGGGGCCATGGGGGTAGCGATGCGCCGGTGGGCGCCTACACGATG
ACGCGGCTGGCCGACGACGTGGTGGCGCTGATGGACGAACTCGATATCGCGCAGGCCCACTTCTGTGGGGTGTCGGTGGG
CGGTATGGTGGCGCAGACGTTGGGCGTGCGCCATCCGGAGCGGCTGTTGTCGCTGACGCTGGTGGACACGATTCACCACA
CGCCGCTGGAAGCCCGGGCGATGTGGGCCGACCGGATCGGACAGGTGGAAGCGCATGGCATGGGCGGGACCGTCGAGTCG
ACGCTGAATCGGTGGCTGACCGCACCGTTCCGGGAGCGGCACCCAGAAATCGTCGAGAGAATCAGGAAAATGTTGCTGGA
AACACCAGTGCGCGGCTATGTTGGTGTAGCGCAGGCCATCGAAGCCTTCGATCTGGCCCGCGCGATCTCCCGGATTCACT
GCCCGACGCTAGTGGTGGTCGGCGACAAGGACGAGGGCTCGCCGGTGTCGATCGCCGAAGCCATCGCCCGTGAGATTCAC
GGTTCAAGGCTGGAAGTGCTGCCGGACGCGGCGCACCTGTCATTCATCGAACAACCGGAACGTTTCCATGCGATGTTCGA
CGCGTTCCTGGGACACGCGGCTTGTGGCGGCCAGTGTGATATTCCGTAA

Upstream 100 bases:

>100_bases
TGGCACTCAGATCGATCTCGTCTATCGTAGTAGTGAGACACATTGACGAATGCGCCTTGGGAGCCGGTGGGTGGCGCCAT
GACCGTACGGAGACTGACAC

Downstream 100 bases:

>100_bases
CGCCATTGGAAATATCGCAACACAGTAGTGCACGGCGCTCAAGAAACTTGCAGGCTGCGCCGATGCCCAATGTAAGAGGG
CAGCAATGGCACGACAAGGT

Product: putative hydrolase

Products: NA

Alternate protein names: 3-oxoadipate enol-lactonase I; Beta-ketoadipate enol-lactone hydrolase I; Enol-lactone hydrolase I [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSDTSASTASQTNRLRVGDVHLQVRVDGTEGPWVILAHALGANLTLWDDTARHLAARYRVLRFDMRGHGGSDAPVGAYTM
TRLADDVVALMDELDIAQAHFCGVSVGGMVAQTLGVRHPERLLSLTLVDTIHHTPLEARAMWADRIGQVEAHGMGGTVES
TLNRWLTAPFRERHPEIVERIRKMLLETPVRGYVGVAQAIEAFDLARAISRIHCPTLVVVGDKDEGSPVSIAEAIAREIH
GSRLEVLPDAAHLSFIEQPERFHAMFDAFLGHAACGGQCDIP

Sequences:

>Translated_282_residues
MSDTSASTASQTNRLRVGDVHLQVRVDGTEGPWVILAHALGANLTLWDDTARHLAARYRVLRFDMRGHGGSDAPVGAYTM
TRLADDVVALMDELDIAQAHFCGVSVGGMVAQTLGVRHPERLLSLTLVDTIHHTPLEARAMWADRIGQVEAHGMGGTVES
TLNRWLTAPFRERHPEIVERIRKMLLETPVRGYVGVAQAIEAFDLARAISRIHCPTLVVVGDKDEGSPVSIAEAIAREIH
GSRLEVLPDAAHLSFIEQPERFHAMFDAFLGHAACGGQCDIP
>Mature_281_residues
SDTSASTASQTNRLRVGDVHLQVRVDGTEGPWVILAHALGANLTLWDDTARHLAARYRVLRFDMRGHGGSDAPVGAYTMT
RLADDVVALMDELDIAQAHFCGVSVGGMVAQTLGVRHPERLLSLTLVDTIHHTPLEARAMWADRIGQVEAHGMGGTVEST
LNRWLTAPFRERHPEIVERIRKMLLETPVRGYVGVAQAIEAFDLARAISRIHCPTLVVVGDKDEGSPVSIAEAIAREIHG
SRLEVLPDAAHLSFIEQPERFHAMFDAFLGHAACGGQCDIP

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012790 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.24 [H]

Molecular weight: Translated: 30754; Mature: 30623

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSASTASQTNRLRVGDVHLQVRVDGTEGPWVILAHALGANLTLWDDTARHLAARYRV
CCCCCCCCHHHCCCEEEEEEEEEEEEECCCCCEEEEEEHHCCCEEEECHHHHHHHHHHHH
LRFDMRGHGGSDAPVGAYTMTRLADDVVALMDELDIAQAHFCGVSVGGMVAQTLGVRHPE
HEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
RLLSLTLVDTIHHTPLEARAMWADRIGQVEAHGMGGTVESTLNRWLTAPFRERHPEIVER
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCHHHCCHHHHHH
IRKMLLETPVRGYVGVAQAIEAFDLARAISRIHCPTLVVVGDKDEGSPVSIAEAIAREIH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GSRLEVLPDAAHLSFIEQPERFHAMFDAFLGHAACGGQCDIP
CCCEEECCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDTSASTASQTNRLRVGDVHLQVRVDGTEGPWVILAHALGANLTLWDDTARHLAARYRV
CCCCCCCHHHCCCEEEEEEEEEEEEECCCCCEEEEEEHHCCCEEEECHHHHHHHHHHHH
LRFDMRGHGGSDAPVGAYTMTRLADDVVALMDELDIAQAHFCGVSVGGMVAQTLGVRHPE
HEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
RLLSLTLVDTIHHTPLEARAMWADRIGQVEAHGMGGTVESTLNRWLTAPFRERHPEIVER
HHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCHHHCCHHHHHH
IRKMLLETPVRGYVGVAQAIEAFDLARAISRIHCPTLVVVGDKDEGSPVSIAEAIAREIH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GSRLEVLPDAAHLSFIEQPERFHAMFDAFLGHAACGGQCDIP
CCCEEECCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181753 [H]