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The map label for this gene is yiaJ [H]

Identifier: 94309027

GI number: 94309027

Start: 89192

End: 90049

Strand: Direct

Name: yiaJ [H]

Synonym: Rmet_0082

Alternate gene names: 94309027

Gene position: 89192-90049 (Clockwise)

Preceding gene: 94309020

Following gene: 94309028

Centisome position: 2.27

GC content: 67.48

Gene sequence:

>858_bases
ATGACCGCAGCCGAAGACGACGATACCCGGGACGATATTGCCGACGACACCAGGCGTGCGGGCATCCAGTCCATCGAGGT
GGGATTCCGGCTGCTGCAGGCGCTGGCCGCGTCGCCGCGCGCGATGATGCTGCGCGACCTGGCCGCGGCGGCGGACATGA
GTCCGGCCAAGGCGCACCGCTATCTGGTTAGCTTCCAGCGGCTGGGCGCCGTGACGCAGGACGCCGTGAGCCAGCGCTAC
GACCTTGGCCCGTTCGCGCTGCAGCTTGGCCTGGCCGGGCTGAACCGGCTCGACCCGGTGCGCAAGGCGCGCCCGATCCT
GTCGCAGCTGCGTGACGAGATCGACCAGACCGCCGGTATCGCCGTGTGGGGCAACCACGGGCCGACCATCGTCCACTGGG
AGGAATCGAGCCATCCGGTCAGCGCCAGCCTGCGGCTCGGTGACGTCATGCCGATGCTCAACTCGGCCACGGGCCGCCTT
TATGGGGCTTATCTGTCGCGCCGCCAGACACTGCCGCTGATCGAGCGGGAACTGGCCACGCGTGGCACCGTCCCACTGCC
TGACATGCCCGTATCGCTGGACGAATATGACGCGATCTGCGCGGACGTGCGAGAGCACGGCGCGGCACGCACGCGCGGCG
GGGTGTTGCCGGGCATCAATGCGATCTCCACGCCCGTGTTCGACGCGAATGGCCATCTGGCGATGGCGCTGATCGTGCTC
GGCGCGCAGAGCGTATTCGATGCAGAATGGGGCGGTACGGTGGATCGGCGCGTGCGCGATATCGCCCGGCAAATCTCTTC
GGAACTCGGTTACCTAGATGGCAACCAACGGGCCGGACAAACCGGTCAAACCTCGTAG

Upstream 100 bases:

>100_bases
GAGTCGAACATAGGCAAATCGGATGGGCCACAGTCTACCGGAGCGGCGCGGGCGCACCGTATAGAATGGCCGGCAATACC
GATACGCTTGCCAGGAACGC

Downstream 100 bases:

>100_bases
TCGGTCGCGGGGCGGCACGCTGAGCTGGTGGCAGCGCCGTCGCAGATGGGTTGTGGTCATTCTGCTGGTGGTGGCCGCCC
ATTTGCTGGCGCTGCTGCGT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAAEDDDTRDDIADDTRRAGIQSIEVGFRLLQALAASPRAMMLRDLAAAADMSPAKAHRYLVSFQRLGAVTQDAVSQRY
DLGPFALQLGLAGLNRLDPVRKARPILSQLRDEIDQTAGIAVWGNHGPTIVHWEESSHPVSASLRLGDVMPMLNSATGRL
YGAYLSRRQTLPLIERELATRGTVPLPDMPVSLDEYDAICADVREHGAARTRGGVLPGINAISTPVFDANGHLAMALIVL
GAQSVFDAEWGGTVDRRVRDIARQISSELGYLDGNQRAGQTGQTS

Sequences:

>Translated_285_residues
MTAAEDDDTRDDIADDTRRAGIQSIEVGFRLLQALAASPRAMMLRDLAAAADMSPAKAHRYLVSFQRLGAVTQDAVSQRY
DLGPFALQLGLAGLNRLDPVRKARPILSQLRDEIDQTAGIAVWGNHGPTIVHWEESSHPVSASLRLGDVMPMLNSATGRL
YGAYLSRRQTLPLIERELATRGTVPLPDMPVSLDEYDAICADVREHGAARTRGGVLPGINAISTPVFDANGHLAMALIVL
GAQSVFDAEWGGTVDRRVRDIARQISSELGYLDGNQRAGQTGQTS
>Mature_284_residues
TAAEDDDTRDDIADDTRRAGIQSIEVGFRLLQALAASPRAMMLRDLAAAADMSPAKAHRYLVSFQRLGAVTQDAVSQRYD
LGPFALQLGLAGLNRLDPVRKARPILSQLRDEIDQTAGIAVWGNHGPTIVHWEESSHPVSASLRLGDVMPMLNSATGRLY
GAYLSRRQTLPLIERELATRGTVPLPDMPVSLDEYDAICADVREHGAARTRGGVLPGINAISTPVFDANGHLAMALIVLG
AQSVFDAEWGGTVDRRVRDIARQISSELGYLDGNQRAGQTGQTS

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=269, Percent_Identity=26.7657992565056, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788131, Length=258, Percent_Identity=25.5813953488372, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082359, Length=246, Percent_Identity=25.2032520325203, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786715, Length=258, Percent_Identity=23.6434108527132, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30778; Mature: 30647

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAEDDDTRDDIADDTRRAGIQSIEVGFRLLQALAASPRAMMLRDLAAAADMSPAKAHR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
YLVSFQRLGAVTQDAVSQRYDLGPFALQLGLAGLNRLDPVRKARPILSQLRDEIDQTAGI
HHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHCCE
AVWGNHGPTIVHWEESSHPVSASLRLGDVMPMLNSATGRLYGAYLSRRQTLPLIERELAT
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RGTVPLPDMPVSLDEYDAICADVREHGAARTRGGVLPGINAISTPVFDANGHLAMALIVL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHH
GAQSVFDAEWGGTVDRRVRDIARQISSELGYLDGNQRAGQTGQTS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAAEDDDTRDDIADDTRRAGIQSIEVGFRLLQALAASPRAMMLRDLAAAADMSPAKAHR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
YLVSFQRLGAVTQDAVSQRYDLGPFALQLGLAGLNRLDPVRKARPILSQLRDEIDQTAGI
HHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHCCE
AVWGNHGPTIVHWEESSHPVSASLRLGDVMPMLNSATGRLYGAYLSRRQTLPLIERELAT
EEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RGTVPLPDMPVSLDEYDAICADVREHGAARTRGGVLPGINAISTPVFDANGHLAMALIVL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHH
GAQSVFDAEWGGTVDRRVRDIARQISSELGYLDGNQRAGQTGQTS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]