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The map label for this gene is nfdA [H]

Identifier: 94308950

GI number: 94308950

Start: 8367

End: 10121

Strand: Reverse

Name: nfdA [H]

Synonym: Rmet_0005

Alternate gene names: 94308950

Gene position: 10121-8367 (Counterclockwise)

Preceding gene: 94308951

Following gene: NA

Centisome position: 0.26

GC content: 64.96

Gene sequence:

>1755_bases
ATGTGCTTCTACTGCAGTCCTGGTGGTGCAGCAGTCCTGCTCGCGCGCGCGTCGCGCCGAGACTTCCTGACGGGCGCGCT
GGCGCTCGGTGCAATGTCCACCTTCGGATTCCCGTCGGCATACGCCGAGAACCCCCCGGCTACGTCTCCTTCGACATCTC
CCTACGCGCAATTCATCTTCACCAACGGGCCGATCTACACCATGGCCAGCGGCTCGCCAACGGCCAAGGCCCTGGCCGTG
CGCGATGGCCGCATCGTTGCCGTTGGCTCGATGGATGCGGTCGCGCAGTTCCGTGGCCCGGACACGCAAACCATCGACCT
CGGTGGACGCACGCTGCTGCCGGGATTTGTCGAACCACACATGCACTCCGCATTGGCCGTGCTCGACGCATGGCTCGACG
TGGGCCCGTTCACCACCACGTCGATTGACCAGGCACTCGAGAAGATCCGCGCCGAGGCGGCAAAGTCCAAACCGGGCGAG
TGGGTCCGCGCCCAGGCGCTCGATCCCTCGCTGATGCCCGGCGCACCCGTCACGCGACAACGGCTCGATGCCGTGGCGCC
CAACAATCCGGTCTTCATCCTGGAATCGAACGGACATATTGCCTACGTCAACAGCGCGGCCCTGAAGCTCGCCAACGTCA
CGCGCGATACGCCGAACCCGGCCCAGGGCCGCTTTGTCCGCGACGACAAGGGCGAACTAACCGGCCGGCTCGAGGAAGGC
CCCGCATACGGCCCCTTTCTGAAAGTCGCCCCGATGCCCAACGCCGCGCAAATGGTGGCCAGCATTCGCAAGCTGTTCGA
CCGCGCCAGCGCCGTGGGCTGTACCAGCCTGCACGATGCCGGCATTGGCACCAGCGGCGTGCATGACCTCGATATCGTCA
ACGCCGCCATGCGCGACGATCCTCCTGTCCGCTATTCGGGCTTCCTGACGTCCGACCGGATGGATGGCTGGGTAGAGCGA
GGCATCAAGCCGGGCGCCGGCACAGATCGCTTCCGGCTACACGGCATCAAGTTCTGGTCCGACGGCTCCAACCAGGCCCG
CACCGGATACCAGCGCGAGCCATACCTCAACTCCACCAGCCGTGGCGCGCTGAATTACACCGCCGAGCAACTGATCGAGG
GCGTACAACGCGCGCACGACCTCGGCTGGCAAGTCGCCATCCACGCCAACGGCGACGCCGCCATCGACACCACGCTGGAC
GTCTACGAACACGTGCTGAAACGCTCACCACGCACGGATCATCGACACCGCATCGAACACTGCAGCGTCCTGCACCCCGA
GCAGATTCAGCGAATGGCAAAGCTCGGCGTGTCGCCGTCATTCCTGATCGGACACGTGCACTACTGGGGCAAGGCATTCC
GCGACAACATCCTCGGCCCCCAGCGCGCCCAGTTCTACGCCCCCTGCGCATCGGCACTGCGCGGCGGCCTGCGCATCTCG
CTCCACTCCGACTACAACGTGACGCCGATCGAACCGATCCGTTACATGGACAACGCCGTCAACCGCACCATGCGCGACGG
CGGTGGCGTGCTCAACCCAGCGGAGCGCATTACCGTGGCGCAGGCACTGCGGGCGGTGACGATCGACGCGGCGTGGCAAT
GCCAGATGGACCAGATCGTTGGCAGCCTGGAGACCGGGAAGTATGCGGATCTGGTGATTGTGGATAAGGACCCGCTCCAG
ATGGCGCAGGAGGAGATTGGTAAGTTGAAGGTGATGGAGACTTGGTCGGAGGGACGGAGGCGGTTTGCGGCTTGA

Upstream 100 bases:

>100_bases
AAAACGCGGATGGACGGTCACCGCTATCGTCCGGCAGGCTACAGTCGACGCCACATACGCGCCATTCCCAACACAGCAAC
GATAACGATGGGGTAACCAC

Downstream 100 bases:

>100_bases
GGGGGGGATGTTGGTGCGGAAATGGCGCTCGGCGTCATTTCCGCTTCAGGTTTGCATGGCTTCGCGGTCTTTTGCTGCCG
CGTTCATGCGCACATCTGTC

Product: putative metal-dependent amidohydrolase (TAT secreted)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MCFYCSPGGAAVLLARASRRDFLTGALALGAMSTFGFPSAYAENPPATSPSTSPYAQFIFTNGPIYTMASGSPTAKALAV
RDGRIVAVGSMDAVAQFRGPDTQTIDLGGRTLLPGFVEPHMHSALAVLDAWLDVGPFTTTSIDQALEKIRAEAAKSKPGE
WVRAQALDPSLMPGAPVTRQRLDAVAPNNPVFILESNGHIAYVNSAALKLANVTRDTPNPAQGRFVRDDKGELTGRLEEG
PAYGPFLKVAPMPNAAQMVASIRKLFDRASAVGCTSLHDAGIGTSGVHDLDIVNAAMRDDPPVRYSGFLTSDRMDGWVER
GIKPGAGTDRFRLHGIKFWSDGSNQARTGYQREPYLNSTSRGALNYTAEQLIEGVQRAHDLGWQVAIHANGDAAIDTTLD
VYEHVLKRSPRTDHRHRIEHCSVLHPEQIQRMAKLGVSPSFLIGHVHYWGKAFRDNILGPQRAQFYAPCASALRGGLRIS
LHSDYNVTPIEPIRYMDNAVNRTMRDGGGVLNPAERITVAQALRAVTIDAAWQCQMDQIVGSLETGKYADLVIVDKDPLQ
MAQEEIGKLKVMETWSEGRRRFAA

Sequences:

>Translated_584_residues
MCFYCSPGGAAVLLARASRRDFLTGALALGAMSTFGFPSAYAENPPATSPSTSPYAQFIFTNGPIYTMASGSPTAKALAV
RDGRIVAVGSMDAVAQFRGPDTQTIDLGGRTLLPGFVEPHMHSALAVLDAWLDVGPFTTTSIDQALEKIRAEAAKSKPGE
WVRAQALDPSLMPGAPVTRQRLDAVAPNNPVFILESNGHIAYVNSAALKLANVTRDTPNPAQGRFVRDDKGELTGRLEEG
PAYGPFLKVAPMPNAAQMVASIRKLFDRASAVGCTSLHDAGIGTSGVHDLDIVNAAMRDDPPVRYSGFLTSDRMDGWVER
GIKPGAGTDRFRLHGIKFWSDGSNQARTGYQREPYLNSTSRGALNYTAEQLIEGVQRAHDLGWQVAIHANGDAAIDTTLD
VYEHVLKRSPRTDHRHRIEHCSVLHPEQIQRMAKLGVSPSFLIGHVHYWGKAFRDNILGPQRAQFYAPCASALRGGLRIS
LHSDYNVTPIEPIRYMDNAVNRTMRDGGGVLNPAERITVAQALRAVTIDAAWQCQMDQIVGSLETGKYADLVIVDKDPLQ
MAQEEIGKLKVMETWSEGRRRFAA
>Mature_584_residues
MCFYCSPGGAAVLLARASRRDFLTGALALGAMSTFGFPSAYAENPPATSPSTSPYAQFIFTNGPIYTMASGSPTAKALAV
RDGRIVAVGSMDAVAQFRGPDTQTIDLGGRTLLPGFVEPHMHSALAVLDAWLDVGPFTTTSIDQALEKIRAEAAKSKPGE
WVRAQALDPSLMPGAPVTRQRLDAVAPNNPVFILESNGHIAYVNSAALKLANVTRDTPNPAQGRFVRDDKGELTGRLEEG
PAYGPFLKVAPMPNAAQMVASIRKLFDRASAVGCTSLHDAGIGTSGVHDLDIVNAAMRDDPPVRYSGFLTSDRMDGWVER
GIKPGAGTDRFRLHGIKFWSDGSNQARTGYQREPYLNSTSRGALNYTAEQLIEGVQRAHDLGWQVAIHANGDAAIDTTLD
VYEHVLKRSPRTDHRHRIEHCSVLHPEQIQRMAKLGVSPSFLIGHVHYWGKAFRDNILGPQRAQFYAPCASALRGGLRIS
LHSDYNVTPIEPIRYMDNAVNRTMRDGGGVLNPAERITVAQALRAVTIDAAWQCQMDQIVGSLETGKYADLVIVDKDPLQ
MAQEEIGKLKVMETWSEGRRRFAA

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 63444; Mature: 63444

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCFYCSPGGAAVLLARASRRDFLTGALALGAMSTFGFPSAYAENPPATSPSTSPYAQFIF
CEEEECCCCCEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
TNGPIYTMASGSPTAKALAVRDGRIVAVGSMDAVAQFRGPDTQTIDLGGRTLLPGFVEPH
ECCCEEEEECCCCCCEEEEEECCEEEEECCHHHHHHHCCCCCCEEECCCCEECCCCCCHH
MHSALAVLDAWLDVGPFTTTSIDQALEKIRAEAAKSKPGEWVRAQALDPSLMPGAPVTRQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHH
RLDAVAPNNPVFILESNGHIAYVNSAALKLANVTRDTPNPAQGRFVRDDKGELTGRLEEG
HHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEECCC
PAYGPFLKVAPMPNAAQMVASIRKLFDRASAVGCTSLHDAGIGTSGVHDLDIVNAAMRDD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PPVRYSGFLTSDRMDGWVERGIKPGAGTDRFRLHGIKFWSDGSNQARTGYQREPYLNSTS
CCCEEECEECCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
RGALNYTAEQLIEGVQRAHDLGWQVAIHANGDAAIDTTLDVYEHVLKRSPRTDHRHRIEH
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHH
CSVLHPEQIQRMAKLGVSPSFLIGHVHYWGKAFRDNILGPQRAQFYAPCASALRGGLRIS
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEE
LHSDYNVTPIEPIRYMDNAVNRTMRDGGGVLNPAERITVAQALRAVTIDAAWQCQMDQIV
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCHHHEEHHHHH
GSLETGKYADLVIVDKDPLQMAQEEIGKLKVMETWSEGRRRFAA
CCCCCCCEEEEEEECCCHHHHHHHHHCCEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MCFYCSPGGAAVLLARASRRDFLTGALALGAMSTFGFPSAYAENPPATSPSTSPYAQFIF
CEEEECCCCCEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
TNGPIYTMASGSPTAKALAVRDGRIVAVGSMDAVAQFRGPDTQTIDLGGRTLLPGFVEPH
ECCCEEEEECCCCCCEEEEEECCEEEEECCHHHHHHHCCCCCCEEECCCCEECCCCCCHH
MHSALAVLDAWLDVGPFTTTSIDQALEKIRAEAAKSKPGEWVRAQALDPSLMPGAPVTRQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHH
RLDAVAPNNPVFILESNGHIAYVNSAALKLANVTRDTPNPAQGRFVRDDKGELTGRLEEG
HHHHCCCCCCEEEEECCCCEEEEEHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEEECCC
PAYGPFLKVAPMPNAAQMVASIRKLFDRASAVGCTSLHDAGIGTSGVHDLDIVNAAMRDD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PPVRYSGFLTSDRMDGWVERGIKPGAGTDRFRLHGIKFWSDGSNQARTGYQREPYLNSTS
CCCEEECEECCCHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
RGALNYTAEQLIEGVQRAHDLGWQVAIHANGDAAIDTTLDVYEHVLKRSPRTDHRHRIEH
CCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHH
CSVLHPEQIQRMAKLGVSPSFLIGHVHYWGKAFRDNILGPQRAQFYAPCASALRGGLRIS
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEE
LHSDYNVTPIEPIRYMDNAVNRTMRDGGGVLNPAERITVAQALRAVTIDAAWQCQMDQIV
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCHHHEEHHHHH
GSLETGKYADLVIVDKDPLQMAQEEIGKLKVMETWSEGRRRFAA
CCCCCCCEEEEEEECCCHHHHHHHHHCCEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA