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The map label for this gene is ribE [H]

Identifier: 93007293

GI number: 93007293

Start: 3053155

End: 3053826

Strand: Reverse

Name: ribE [H]

Synonym: Pcryo_2469

Alternate gene names: 93007293

Gene position: 3053826-3053155 (Counterclockwise)

Preceding gene: 93007294

Following gene: 93007291

Centisome position: 99.8

GC content: 42.41

Gene sequence:

>672_bases
ATGTTTACCGGAATTATAGAAAGTGTGGGAAAGGTTAAGTCGATGCAACCTACAGGCGGCGACATTCGCTTGACGATAGA
GTCTGATGGCTTAGATTTTAGCGATGTGAAGCTTGGAGACTCTATAGCTTCTAACGGTATCTGTCTTACGGTTGTAGATT
TCGGTAGTAATCATTATTCCGTTGATGTTTCACGTGAAACTATAGCGCGAACAGCATTAGAAGATCTAAAGCCCGGTCAT
CTGGTCAATTTAGAAAAAGCGATGCTGCCAACCACACGTTTTGGTGGTCATATCGTCGCAGGTCATGTCGATGGCGTCGG
GATAGTCAGTAAGCTACAAAAGGATGCGCGCTCTATTTATATCGAAATTGAGATACCAAAAGAGTTAGTACATTATACCG
CGACCAAAGGCTCGATTACCCTTGATGGTATTAGCCTTACCACCAATTTAGTACGCGATAATATCGTGTCGCTCAATATC
ATTCCACATACTGCGCAAGTGACCAATATTGCTCAGCATTGGCTGATAGGATCTAAGGTCAATGTAGAGGTAGATATCGT
GGCCAGATATTTAGAGCGTTTATTAAATAAGTCGCAGGGAACCAGTGACAATAGCAGCCCGCAGAGCAGTATTACTGAAG
CTTTTTTAGCGGATAATGGTTTTATGAAATAA

Upstream 100 bases:

>100_bases
TCACTTATCCACAGTTTACCCCGAAATATATGTGATATAGCATATTTATTACTGAGAAAAGCTATCTATAACGACAATAA
AATAATGAGAAGAGGTTGAT

Downstream 100 bases:

>100_bases
GACCTTTGCTACAACAGTCTTATTCTGCTGCAAATTTTATATTAGCAGTAAGTGAGAAGCATGGAAGCTATCTTGTTTTG
GCATGGTTTTGTGTAAGTGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MFTGIIESVGKVKSMQPTGGDIRLTIESDGLDFSDVKLGDSIASNGICLTVVDFGSNHYSVDVSRETIARTALEDLKPGH
LVNLEKAMLPTTRFGGHIVAGHVDGVGIVSKLQKDARSIYIEIEIPKELVHYTATKGSITLDGISLTTNLVRDNIVSLNI
IPHTAQVTNIAQHWLIGSKVNVEVDIVARYLERLLNKSQGTSDNSSPQSSITEAFLADNGFMK

Sequences:

>Translated_223_residues
MFTGIIESVGKVKSMQPTGGDIRLTIESDGLDFSDVKLGDSIASNGICLTVVDFGSNHYSVDVSRETIARTALEDLKPGH
LVNLEKAMLPTTRFGGHIVAGHVDGVGIVSKLQKDARSIYIEIEIPKELVHYTATKGSITLDGISLTTNLVRDNIVSLNI
IPHTAQVTNIAQHWLIGSKVNVEVDIVARYLERLLNKSQGTSDNSSPQSSITEAFLADNGFMK
>Mature_223_residues
MFTGIIESVGKVKSMQPTGGDIRLTIESDGLDFSDVKLGDSIASNGICLTVVDFGSNHYSVDVSRETIARTALEDLKPGH
LVNLEKAMLPTTRFGGHIVAGHVDGVGIVSKLQKDARSIYIEIEIPKELVHYTATKGSITLDGISLTTNLVRDNIVSLNI
IPHTAQVTNIAQHWLIGSKVNVEVDIVARYLERLLNKSQGTSDNSSPQSSITEAFLADNGFMK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=201, Percent_Identity=33.8308457711443, Blast_Score=104, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319733, Length=204, Percent_Identity=37.2549019607843, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24089; Mature: 24089

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIESVGKVKSMQPTGGDIRLTIESDGLDFSDVKLGDSIASNGICLTVVDFGSNHYS
CCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEECCCHHHCCCEEEEEEECCCCEEE
VDVSRETIARTALEDLKPGHLVNLEKAMLPTTRFGGHIVAGHVDGVGIVSKLQKDARSIY
EEECHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCEEEE
IEIEIPKELVHYTATKGSITLDGISLTTNLVRDNIVSLNIIPHTAQVTNIAQHWLIGSKV
EEEECCHHHHHEECCCCCEEECCHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCC
NVEVDIVARYLERLLNKSQGTSDNSSPQSSITEAFLADNGFMK
EEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFTGIIESVGKVKSMQPTGGDIRLTIESDGLDFSDVKLGDSIASNGICLTVVDFGSNHYS
CCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEECCCHHHCCCEEEEEEECCCCEEE
VDVSRETIARTALEDLKPGHLVNLEKAMLPTTRFGGHIVAGHVDGVGIVSKLQKDARSIY
EEECHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCEEEE
IEIEIPKELVHYTATKGSITLDGISLTTNLVRDNIVSLNIIPHTAQVTNIAQHWLIGSKV
EEEECCHHHHHEECCCCCEEECCHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCC
NVEVDIVARYLERLLNKSQGTSDNSSPQSSITEAFLADNGFMK
EEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]