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The map label for this gene is lip2 [H]

Identifier: 93007282

GI number: 93007282

Start: 3037053

End: 3038504

Strand: Direct

Name: lip2 [H]

Synonym: Pcryo_2458

Alternate gene names: 93007282

Gene position: 3037053-3038504 (Clockwise)

Preceding gene: 93007279

Following gene: 93007292

Centisome position: 99.25

GC content: 42.84

Gene sequence:

>1452_bases
ATGTCTAACTCAACAGTACTATCAGTCAACACCTTACTCAATAAAGCGGTGAAGACCCTAAATCTTATGTCATTTGGACA
AGATAAGAACCCCAAAAGCACTGATATCAATATATCAGCTGAAATAATGGATATAGATGAGAGTGCTTTGCAAGATAGTC
GCGAGGATAAAGGCTTATCTATTAAAGAAAAGATACTCGAGCACCATCTGATGACCAACTATCAACCGCATCTACTGCAC
TATGCCATAAAAAGCTTTGGCTGCTTACCTACGCCGATTCTTGAGAGTTTGATAAAGTGTTTAGATGGACCTACTTCAAA
GCAATACTTGCATGTTGATGCCCATCTGCGCTTAATACTTGCAGTCAATAGCAAGCTAAAAACGCCTTTGCAGCTGATAG
AGATGTCTGAGCTGCGTAAACGCTTTGCAACAGATGCGGTCGCTATGCAAGCACCAAAAGTATGGCAGCAGGCTTCTGAC
AACTTGCTTAGCAATTTGAAACAGTTTCATAAAAAAGGCGATAGCGCCATCAGTTGGCAAGATAGAACGATTGCCAATGC
CGACGACGGTGATATGACCATTCGCTGCTATCAAAATGAGACCTCTGATAATGGTTTTGGCTTTAAAAAAGAGCAGACCA
GTAATCCTGATGAGACCGTGCTATTGTTTTTTCATGGTGGTGGATTTTGTATTGGTGACCTTAATACCCATCATGAATTT
TGTCATGCTATTTGTGAGCAGACGGGTTGGCCGGTCATTAGTGTTGATTATCGCTTAGCACCTGAACACCCTGCCCCTGC
TGCCGTAAGAGACTGTATCAGTGCCTATGCTTGGTTAGCTGAACATTGTGAAGAATTTGGTGCTTTGCCATCACGCATTG
TATTAGCAGGTGATAGCGCAGGTGGTGGATTATCTACTTTGATGGCTCAGCAAATCATCACGCCAAATAAAGAGGCTTGG
TTGGATCTAGGTGATGAAGGTCAAAAGACCTTTGATATATTACAAGGTTTACCACATCCTATGGCACAGATGCCCTTATA
TCCTGTGACCGATATTGAGACCGATTATCCAAGCTGGGAATTATATGGTGAAGGTCTACTACTCGACCATGCTGATGTTG
CCATTTTTGATGCCGCTTGTTTAGAAAATAGTCCGTTACCGCGTCAGCATATCCTGACCTCACCGATGCTTGGGGACAAT
CGACAGGTTTGTCCCAGTTATGTCGTTGCAGCAGAATTAGATGTCTTACGTGATGAAGCGTTTGCTTATGCCGATCAGCT
AAAGAGTTATGGTATAGCCGTACAAACCCATACGGTACTTGGCGCACCGCATGGATTTATTCACTTTATGAGCGTTCATC
AAAGATTAGGGCAAGAAACTCAGCATATCATCACAGGGTTTGCTAATTTTGTGCGTGAAATCATAAAAACAAGGGCGCTA
TTGAGCGCTTAA

Upstream 100 bases:

>100_bases
GACTGCATAACAGTCATTACACATTTGCCAAATGTCAGCAAAATACTAACAGTGAATACTTACCATTAATAAGTATTCAC
CATGATTTTTAAGAGGTGTT

Downstream 100 bases:

>100_bases
TGAGAATTTTAATATTTGAGTGCTTATTGATCTTAAGTTTGAGTACCTACATTGGTACCTGTAAGATTGATTATCCAAAT
CTCTGATCGGGAGCCTAGTC

Product: alpha/beta hydrolase fold protein-3

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINISAEIMDIDESALQDSREDKGLSIKEKILEHHLMTNYQPHLLH
YAIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLILAVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASD
NLLSNLKQFHKKGDSAISWQDRTIANADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEF
CHAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSAGGGLSTLMAQQIITPNKEAW
LDLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWELYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDN
RQVCPSYVVAAELDVLRDEAFAYADQLKSYGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRAL
LSA

Sequences:

>Translated_483_residues
MSNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINISAEIMDIDESALQDSREDKGLSIKEKILEHHLMTNYQPHLLH
YAIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLILAVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASD
NLLSNLKQFHKKGDSAISWQDRTIANADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEF
CHAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSAGGGLSTLMAQQIITPNKEAW
LDLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWELYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDN
RQVCPSYVVAAELDVLRDEAFAYADQLKSYGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRAL
LSA
>Mature_482_residues
SNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINISAEIMDIDESALQDSREDKGLSIKEKILEHHLMTNYQPHLLHY
AIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLILAVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASDN
LLSNLKQFHKKGDSAISWQDRTIANADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEFC
HAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSAGGGLSTLMAQQIITPNKEAWL
DLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWELYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDNR
QVCPSYVVAAELDVLRDEAFAYADQLKSYGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRALL
SA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=97, Percent_Identity=40.2061855670103, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI157041239, Length=133, Percent_Identity=30.8270676691729, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI61966717, Length=306, Percent_Identity=23.8562091503268, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI206597554, Length=309, Percent_Identity=26.537216828479, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI68299767, Length=292, Percent_Identity=24.3150684931507, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI157041237, Length=80, Percent_Identity=35, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1786682, Length=271, Percent_Identity=28.0442804428044, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=319, Percent_Identity=27.2727272727273, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=206, Percent_Identity=28.1553398058252, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=167, Percent_Identity=30.5389221556886, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=154, Percent_Identity=30.5194805194805, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=92, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=92, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=38.7755102040816, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=38.7755102040816, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=38.7755102040816, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 53574; Mature: 53443

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINISAEIMDIDESALQDSREDKGLS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCCCCC
IKEKILEHHLMTNYQPHLLHYAIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLIL
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEECHHEEEEE
AVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASDNLLSNLKQFHKKGDSAISWQ
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DRTIANADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEF
CCEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCHHHHH
CHAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSA
HHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GGGLSTLMAQQIITPNKEAWLDLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWE
CCHHHHHHHHHHCCCCHHHHEECCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCE
LYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDNRQVCPSYVVAAELDVLRDEA
EECCCEEEECCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAYADQLKSYGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRAL
HHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LSA
HCC
>Mature Secondary Structure 
SNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINISAEIMDIDESALQDSREDKGLS
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCCCCC
IKEKILEHHLMTNYQPHLLHYAIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLIL
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEECHHEEEEE
AVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASDNLLSNLKQFHKKGDSAISWQ
EECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DRTIANADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEF
CCEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCHHHHH
CHAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSA
HHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
GGGLSTLMAQQIITPNKEAWLDLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWE
CCHHHHHHHHHHCCCCHHHHEECCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCE
LYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDNRQVCPSYVVAAELDVLRDEA
EECCCEEEECCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FAYADQLKSYGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRAL
HHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LSA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]