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The map label for this gene is tesA [C]

Identifier: 93007258

GI number: 93007258

Start: 3007778

End: 3008590

Strand: Direct

Name: tesA [C]

Synonym: Pcryo_2434

Alternate gene names: 93007258

Gene position: 3007778-3008590 (Clockwise)

Preceding gene: 93007257

Following gene: 93007259

Centisome position: 98.3

GC content: 44.4

Gene sequence:

>813_bases
ATGATGAACAGACCTTTTCGCTTGCCGCAGCGCTTGCTCACGGTTGTCGCATTGACGGTAGCAATCGCAATCAGTGGTTG
CCAAAAAACGCCTGATGCTAATGAAGCTCAGACGGATACTGGCAGTCAAAACAGCAATAAAAATACCAGTCAAACCTCCG
ATATCCAAGTGTCTCAAACCACATCTACAACGAACCCTGCTGAACAACCAGCGCCTTTGACCATATTGGCATTAGGTGAC
TCGTTAACCGAAGGGCTTGGCGTTGATAATGATGACAATTACCCTGCTCAATTACAAACACGCTTACAAGAACTTGGTTA
TAAAGATGTAAAAGTCATCAATTCAGGATTGAGCGGCGAGACCAGTACCGGTTTGGTCAATCGCTTGGATTGGGTATTAC
AGACCAAACCTGATATCACTATTTTGACCATTGGTGCCAATGATGCTATTCGTGGTATTGATGTCGCGACGGTTGAAGCA
AATATTCGTACTGCCGTAAAACGCTTACAGGACAATGACAGCGTGGTTATCTTGGGCGGTATGCAGATCTATGACAACCT
TGGCTCTGATTATGTAAAATCTTTTGCAGCGATATATCCAAGAGTGGCGAAAGACATGAACGTGACGCTTATTCCGTTCT
TTTTAGACGGTGTTGGCGGCGACCCTAAGCTCAATCAAGCCGATGCCATTCATCCGACCAAAGAAGGTTATACGCTGATC
GTTAATAACAATATCTTGCCGATATTACAGCCTGAGCTTAAAAAAATAAATCCGTCGAATGTGACCAATACGCCTACTGA
GACAGCACAATGA

Upstream 100 bases:

>100_bases
GAAGGTTTAGTTTTTATCATTAATCGATTAACTTTTGCACCATATTGCTGTTTATCTGATTCTGCTAGACTGGCTCTATT
GCCTATAGGACAGCAGAATT

Downstream 100 bases:

>100_bases
CATCAGCATTACCTACCTCGCAAGCAGCGCTGCTCACTGCCACTAAGCTAAATAAAACCGTACACGTTGGTGACCAGTCC
TTGTCTATTATTAAAGACGT

Product: G-D-S-L lipolytic protein

Products: NA

Alternate protein names: Aryl-ester hydrolase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MMNRPFRLPQRLLTVVALTVAIAISGCQKTPDANEAQTDTGSQNSNKNTSQTSDIQVSQTTSTTNPAEQPAPLTILALGD
SLTEGLGVDNDDNYPAQLQTRLQELGYKDVKVINSGLSGETSTGLVNRLDWVLQTKPDITILTIGANDAIRGIDVATVEA
NIRTAVKRLQDNDSVVILGGMQIYDNLGSDYVKSFAAIYPRVAKDMNVTLIPFFLDGVGGDPKLNQADAIHPTKEGYTLI
VNNNILPILQPELKKINPSNVTNTPTETAQ

Sequences:

>Translated_270_residues
MMNRPFRLPQRLLTVVALTVAIAISGCQKTPDANEAQTDTGSQNSNKNTSQTSDIQVSQTTSTTNPAEQPAPLTILALGD
SLTEGLGVDNDDNYPAQLQTRLQELGYKDVKVINSGLSGETSTGLVNRLDWVLQTKPDITILTIGANDAIRGIDVATVEA
NIRTAVKRLQDNDSVVILGGMQIYDNLGSDYVKSFAAIYPRVAKDMNVTLIPFFLDGVGGDPKLNQADAIHPTKEGYTLI
VNNNILPILQPELKKINPSNVTNTPTETAQ
>Mature_270_residues
MMNRPFRLPQRLLTVVALTVAIAISGCQKTPDANEAQTDTGSQNSNKNTSQTSDIQVSQTTSTTNPAEQPAPLTILALGD
SLTEGLGVDNDDNYPAQLQTRLQELGYKDVKVINSGLSGETSTGLVNRLDWVLQTKPDITILTIGANDAIRGIDVATVEA
NIRTAVKRLQDNDSVVILGGMQIYDNLGSDYVKSFAAIYPRVAKDMNVTLIPFFLDGVGGDPKLNQADAIHPTKEGYTLI
VNNNILPILQPELKKINPSNVTNTPTETAQ

Specific function: Favors the hydrolysis of several arylesters [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=170, Percent_Identity=31.7647058823529, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.2 [H]

Molecular weight: Translated: 29074; Mature: 29074

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNRPFRLPQRLLTVVALTVAIAISGCQKTPDANEAQTDTGSQNSNKNTSQTSDIQVSQT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
TSTTNPAEQPAPLTILALGDSLTEGLGVDNDDNYPAQLQTRLQELGYKDVKVINSGLSGE
CCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCC
TSTGLVNRLDWVLQTKPDITILTIGANDAIRGIDVATVEANIRTAVKRLQDNDSVVILGG
CHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEC
MQIYDNLGSDYVKSFAAIYPRVAKDMNVTLIPFFLDGVGGDPKLNQADAIHPTKEGYTLI
HHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
VNNNILPILQPELKKINPSNVTNTPTETAQ
ECCCCCCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMNRPFRLPQRLLTVVALTVAIAISGCQKTPDANEAQTDTGSQNSNKNTSQTSDIQVSQT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
TSTTNPAEQPAPLTILALGDSLTEGLGVDNDDNYPAQLQTRLQELGYKDVKVINSGLSGE
CCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCC
TSTGLVNRLDWVLQTKPDITILTIGANDAIRGIDVATVEANIRTAVKRLQDNDSVVILGG
CHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEC
MQIYDNLGSDYVKSFAAIYPRVAKDMNVTLIPFFLDGVGGDPKLNQADAIHPTKEGYTLI
HHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
VNNNILPILQPELKKINPSNVTNTPTETAQ
ECCCCCCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8141782 [H]