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The map label for this gene is parE [H]

Identifier: 93007238

GI number: 93007238

Start: 2971081

End: 2972967

Strand: Reverse

Name: parE [H]

Synonym: Pcryo_2414

Alternate gene names: 93007238

Gene position: 2972967-2971081 (Counterclockwise)

Preceding gene: 93007239

Following gene: 93007237

Centisome position: 97.16

GC content: 46.79

Gene sequence:

>1887_bases
GTGAGTCAATATAATGCGCAATCGTTAGAAGTTTTAGAAGGTCTTGAGCCAGTCCGTCGTCGTCCAGGTATGTACACCGA
TACCACGCGCCCGAACCATTTGGCGCAAGAGGTCATTGATAACTCGGTTGATGAGGCGTTAGCGGGTTATGCTAAAACCA
TCAAAGTGCAACTGCATAGTGATGGCTCGTTGTCTGTCGAAGATGATGGACGCGGTATGCCAACAGATATTCATCCTGAA
TTTAACCAATCAGGGATTGAGCTGATTTTAACTCGTTTGCATGCAGGTGGGAAATTTTCGACTTCCAATTATGAGGTGTC
AGGTGGCTTGCATGGCGTGGGTATCTCTGTCGTCAATGCGCTATCAACGCGTGTTGAGGTTACGGTATGGCGTGATAGCA
CACAGTTTGATATGGCGTTTGAAAATGGTGCGCCCGTTACGCCGTTGACCGAAGCGGTTAGCTCAAATAAGCGCAAACGC
GGTACTAAAGTGCACTTTTGGGCAGATGGTAGTTTCTTTGATACGCCAAAATTTAATGTAAAGCAGCTCAAGCATAATTT
GAAAGCCAAGGCGGTTTTGGCAGCTGGACTGACGATTGAATTCACCGATGATATTAATGATGAAAAGGTGGTTTGGCAGT
TTACTGATGGGGTGGTCGAGTATTTAAGCGAACAGTTAGATGGTCTTGAGACATTACCTGAAGCGCCATTCTATTATAAT
TACGATGCTAAAGCGGGTGAACGAGCTGCTATTACCTTTGCATTGTCTTGGTTGCCTGATGGTGGGACGCCAGTTCAAGA
AAGTTATGTAAACCTGATTCCTACAGCTCAGGGCGGTACGCACGTCAATGGACTGCGCACGGGTATCTTAGAAGCCTTGC
GTGAGTTCTGCGATATCCATAATTTACTACCGCGTAGTGTAAAGCTAACCGCAGAAGATGTCTGGGATGGGGTGAACTAT
ATCCTGTCTCTTAAGTTCTCTGAGCCGCAGTTTTCTGGACAGACCAAAGAGCGCTTATCTAGCCGTGAGGCTGCAGGAGC
CGTGCAGACATTGGCAAAAGATGCCTTTAGTCTTTGGCTAAACCAACATGCCGATATGGGTACGCAAATTGCCGAATTGG
CAATTAGCAAAGCAGGACGTCGTTTAAAATCGGCAAAAAAAGTCGCTCGTAAGAAAATCACTCAGGGGCCAGCATTGCCC
GGTAAATTGGCAGATTGCCGCGGTGATTTGCGTGATGGGGCTGAGCTGTTTTTGGTAGAAGGGGATTCTGCGGGTGGTAG
TGCGAAGCAAGCACGTGATCGTCATTACCAAGCGATTCTACCCTTGCGTGGTAAGATCTTAAATACGTGGGAGGTCTCAT
CAGATACAGTGCTCTCGAGTCAAGAAATTCATGATATTGCGATTGCCATTGGCGTTGATCCGGCATCTGACGATTTATCT
GAGCTACGTTATGACAAGGTATGCATCCTAGCGGATGCCGATTCTGATGGACTGCATATTGCTACCTTGATCTGTGCGCT
ATTCGTGAAGCACTTTCCTGCCTTAGTCGATGCTGGTCATTTATTTGTCGCAATGCCGCCACTCTATCGCGTCGATGTGG
CTAAAGAAGTGCATTATGCGTTAGATGAAACCGAGCTTCAGCATATTTTAGCCAATGTACCGGGCAATAAAAAAGCCCAA
ATCACACGTTTTAAAGGCTTGGGTGAGATGAGTGCCGAGCAGCTGCGTGAAACGACCATGAACCGTGATACACGCCGTCT
AGTCCAATTGGATATGGATGACATGGTACTAACCAACAGTGTGATGGATATGCTGCTAGCCAAAAAGCGTGCTGCTGATC
GTAAGATATGGCTAGAAGATAAAGGTAATCTAGCCGATATCTCATAG

Upstream 100 bases:

>100_bases
TTATATGACGTAAGCGATGGACTGTTAACGTTAATGTCGCTATTTTTCGTTATAATGGTTTAATAGAATTAAACGCACGC
GAACAGTTAAGGATGCATTT

Downstream 100 bases:

>100_bases
CTATTTTTAGGACGCTTAGCATTATTTATAATCATGATTGAGTAAGCTATAACATAACAAAAGCATGTAAAATCCAGATT
AGTGATACCAGCTTAATGGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MSQYNAQSLEVLEGLEPVRRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYAKTIKVQLHSDGSLSVEDDGRGMPTDIHPE
FNQSGIELILTRLHAGGKFSTSNYEVSGGLHGVGISVVNALSTRVEVTVWRDSTQFDMAFENGAPVTPLTEAVSSNKRKR
GTKVHFWADGSFFDTPKFNVKQLKHNLKAKAVLAAGLTIEFTDDINDEKVVWQFTDGVVEYLSEQLDGLETLPEAPFYYN
YDAKAGERAAITFALSWLPDGGTPVQESYVNLIPTAQGGTHVNGLRTGILEALREFCDIHNLLPRSVKLTAEDVWDGVNY
ILSLKFSEPQFSGQTKERLSSREAAGAVQTLAKDAFSLWLNQHADMGTQIAELAISKAGRRLKSAKKVARKKITQGPALP
GKLADCRGDLRDGAELFLVEGDSAGGSAKQARDRHYQAILPLRGKILNTWEVSSDTVLSSQEIHDIAIAIGVDPASDDLS
ELRYDKVCILADADSDGLHIATLICALFVKHFPALVDAGHLFVAMPPLYRVDVAKEVHYALDETELQHILANVPGNKKAQ
ITRFKGLGEMSAEQLRETTMNRDTRRLVQLDMDDMVLTNSVMDMLLAKKRAADRKIWLEDKGNLADIS

Sequences:

>Translated_628_residues
MSQYNAQSLEVLEGLEPVRRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYAKTIKVQLHSDGSLSVEDDGRGMPTDIHPE
FNQSGIELILTRLHAGGKFSTSNYEVSGGLHGVGISVVNALSTRVEVTVWRDSTQFDMAFENGAPVTPLTEAVSSNKRKR
GTKVHFWADGSFFDTPKFNVKQLKHNLKAKAVLAAGLTIEFTDDINDEKVVWQFTDGVVEYLSEQLDGLETLPEAPFYYN
YDAKAGERAAITFALSWLPDGGTPVQESYVNLIPTAQGGTHVNGLRTGILEALREFCDIHNLLPRSVKLTAEDVWDGVNY
ILSLKFSEPQFSGQTKERLSSREAAGAVQTLAKDAFSLWLNQHADMGTQIAELAISKAGRRLKSAKKVARKKITQGPALP
GKLADCRGDLRDGAELFLVEGDSAGGSAKQARDRHYQAILPLRGKILNTWEVSSDTVLSSQEIHDIAIAIGVDPASDDLS
ELRYDKVCILADADSDGLHIATLICALFVKHFPALVDAGHLFVAMPPLYRVDVAKEVHYALDETELQHILANVPGNKKAQ
ITRFKGLGEMSAEQLRETTMNRDTRRLVQLDMDDMVLTNSVMDMLLAKKRAADRKIWLEDKGNLADIS
>Mature_627_residues
SQYNAQSLEVLEGLEPVRRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYAKTIKVQLHSDGSLSVEDDGRGMPTDIHPEF
NQSGIELILTRLHAGGKFSTSNYEVSGGLHGVGISVVNALSTRVEVTVWRDSTQFDMAFENGAPVTPLTEAVSSNKRKRG
TKVHFWADGSFFDTPKFNVKQLKHNLKAKAVLAAGLTIEFTDDINDEKVVWQFTDGVVEYLSEQLDGLETLPEAPFYYNY
DAKAGERAAITFALSWLPDGGTPVQESYVNLIPTAQGGTHVNGLRTGILEALREFCDIHNLLPRSVKLTAEDVWDGVNYI
LSLKFSEPQFSGQTKERLSSREAAGAVQTLAKDAFSLWLNQHADMGTQIAELAISKAGRRLKSAKKVARKKITQGPALPG
KLADCRGDLRDGAELFLVEGDSAGGSAKQARDRHYQAILPLRGKILNTWEVSSDTVLSSQEIHDIAIAIGVDPASDDLSE
LRYDKVCILADADSDGLHIATLICALFVKHFPALVDAGHLFVAMPPLYRVDVAKEVHYALDETELQHILANVPGNKKAQI
TRFKGLGEMSAEQLRETTMNRDTRRLVQLDMDDMVLTNSVMDMLLAKKRAADRKIWLEDKGNLADIS

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=625, Percent_Identity=25.44, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI19913406, Length=643, Percent_Identity=24.7278382581649, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1789408, Length=631, Percent_Identity=64.3423137876387, Blast_Score=835, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=643, Percent_Identity=36.0808709175739, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535065, Length=631, Percent_Identity=24.5641838351823, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=548, Percent_Identity=25.3649635036496, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=248, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=641, Percent_Identity=25.585023400936, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17136538, Length=622, Percent_Identity=24.2765273311897, Blast_Score=116, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69085; Mature: 68954

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYNAQSLEVLEGLEPVRRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYAKTIKVQLHS
CCCCCHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCEEEEEEEEEC
DGSLSVEDDGRGMPTDIHPEFNQSGIELILTRLHAGGKFSTSNYEVSGGLHGVGISVVNA
CCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHH
LSTRVEVTVWRDSTQFDMAFENGAPVTPLTEAVSSNKRKRGTKVHFWADGSFFDTPKFNV
HHCEEEEEEEECCCCEEEEECCCCCCCHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCH
KQLKHNLKAKAVLAAGLTIEFTDDINDEKVVWQFTDGVVEYLSEQLDGLETLPEAPFYYN
HHHHHHHHHHEEEEECCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEC
YDAKAGERAAITFALSWLPDGGTPVQESYVNLIPTAQGGTHVNGLRTGILEALREFCDIH
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
NLLPRSVKLTAEDVWDGVNYILSLKFSEPQFSGQTKERLSSREAAGAVQTLAKDAFSLWL
HHCCCCEEEEHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NQHADMGTQIAELAISKAGRRLKSAKKVARKKITQGPALPGKLADCRGDLRDGAELFLVE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEE
GDSAGGSAKQARDRHYQAILPLRGKILNTWEVSSDTVLSSQEIHDIAIAIGVDPASDDLS
CCCCCCCHHHHHHHHHEEEECCCCEEEEEEECCCCCEECCCCCEEEEEEEECCCCCCHHH
ELRYDKVCILADADSDGLHIATLICALFVKHFPALVDAGHLFVAMPPLYRVDVAKEVHYA
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
LDETELQHILANVPGNKKAQITRFKGLGEMSAEQLRETTMNRDTRRLVQLDMDDMVLTNS
HHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHH
VMDMLLAKKRAADRKIWLEDKGNLADIS
HHHHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
SQYNAQSLEVLEGLEPVRRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYAKTIKVQLHS
CCCCHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCEEEEEEEEEC
DGSLSVEDDGRGMPTDIHPEFNQSGIELILTRLHAGGKFSTSNYEVSGGLHGVGISVVNA
CCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHH
LSTRVEVTVWRDSTQFDMAFENGAPVTPLTEAVSSNKRKRGTKVHFWADGSFFDTPKFNV
HHCEEEEEEEECCCCEEEEECCCCCCCHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCH
KQLKHNLKAKAVLAAGLTIEFTDDINDEKVVWQFTDGVVEYLSEQLDGLETLPEAPFYYN
HHHHHHHHHHEEEEECCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEC
YDAKAGERAAITFALSWLPDGGTPVQESYVNLIPTAQGGTHVNGLRTGILEALREFCDIH
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
NLLPRSVKLTAEDVWDGVNYILSLKFSEPQFSGQTKERLSSREAAGAVQTLAKDAFSLWL
HHCCCCEEEEHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NQHADMGTQIAELAISKAGRRLKSAKKVARKKITQGPALPGKLADCRGDLRDGAELFLVE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEE
GDSAGGSAKQARDRHYQAILPLRGKILNTWEVSSDTVLSSQEIHDIAIAIGVDPASDDLS
CCCCCCCHHHHHHHHHEEEECCCCEEEEEEECCCCCEECCCCCEEEEEEEECCCCCCHHH
ELRYDKVCILADADSDGLHIATLICALFVKHFPALVDAGHLFVAMPPLYRVDVAKEVHYA
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH
LDETELQHILANVPGNKKAQITRFKGLGEMSAEQLRETTMNRDTRRLVQLDMDDMVLTNS
HHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHH
VMDMLLAKKRAADRKIWLEDKGNLADIS
HHHHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]