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The map label for this gene is recG [H]

Identifier: 93007226

GI number: 93007226

Start: 2955973

End: 2958321

Strand: Reverse

Name: recG [H]

Synonym: Pcryo_2402

Alternate gene names: 93007226

Gene position: 2958321-2955973 (Counterclockwise)

Preceding gene: 93007228

Following gene: 93007225

Centisome position: 96.68

GC content: 46.19

Gene sequence:

>2349_bases
ATGCCCACATCGGTTAACCCTGCTACACCTCATTTACTAGACGTTTTGGCACTTGATATGCCGGTGACGGCGCTGGCAGG
TGTTGGACCAAAAGTGGCCGAGCAATTGGCGCAATTGGGCATTGCCCGTATCTTTGATTTGCTACTACATCTGCCACGCG
ACTATGAAGATCGTAGTCGCTTGGTATCAATAGGCGATGTGGCTCATGGACAAGCCGCCATGATTACTGGTCGCGTCGTG
CATGTCGATACCAATCGCAGCGGCATGACGGTGATTGTAGATGATGACACGGGTACGATATCTCTACGCTTTTTTAAGGT
ATATCGTGGACTTGCTCAAACCATGTGTCTAGGTACCCATCTACAATTATTCGGTGAAGTCAAAGTGAGCCGCTACGGTA
AGCAGATACATCATCCTGAATATCAAATTATCAGCAGTAACGAGACGGTAGTAAATACGGGTTTACAGCCTATTTATCCA
AGCGTTAAAGGTTTACATCAAAATAAGCTGCGTACATTAATTAAGCTTGCACTGCAAACGGTTCGTAGTCAGGGCTTGCC
GATGACTTTATTTACGCCTGAGGATTTTTCAGTCGTTGCGGATTTGCCATTAGTGCCTTTTGAACCAGCTAAAATACCGA
TAACCGAGCCAGATTATCCAGAAGATATCTTTTCCACCCTCGCGCGTAGTGTGCCTGACAACGGTTTTAGTAGTAGTGAT
AGCAGTAACAGTAGCGTCAATAGAACGAAGCCCTCTTTTTATCATAGTCAAAATAATACCAGTTCCGATGCTATAAGTAT
GGCTAATAATAACGTCTATAATTTAACCATATTTGAAGCGTTGGTATTACTGCATACGCCGCCTACTTATACTGATGCTG
GTCAACAATACAAATTATTAACCCAATTAAGCGCTCGTACTCATGCTGCTTGTCAACGCCTCATTATTGAAGAGTTGACT
GCACATCAGCTCAGCTTATTATATCGCCGTCAGCAATTGCATCAGTATAAAGCGCCAAAATGTGCTATCCAAAGTCCATT
GGCAGATAAATTGTTTGGTGCGTTGCCATTTGATTTAACGGGTGCACAAAAAAGAGTTATGAGGGACATTACCGCTGATA
TGGCGACTTCAATACCAATGCTCAGATTGGTACAAGGCGATGTTGGCGCTGGTAAAACCTTAGTCGCGGCAGGCGCGGCG
GGTTATGCATTAGATAGTGGTTGGCAGGTGGCGGTGATGGCACCGACGGAGATTTTAGCGGAACAGCATTTGGTTAATTT
CAAAAACTGGTTTGAGCCGTTAGGTATTGGGGTTGGTTGGTTGGCTGGTAAACAAACCGCTAAGCAGCGCCGCGAAGCGT
TAGAAGCGGTGAGCGAGAATACCGTACAAGTAGTCGTTGGTACTCATGCGCTTTTCCAGGATCAGGTACAATTTGCCAAA
TTGGGTTTGGTTATTATCGATGAACAGCATCGTTTTGGTGTTGAGCAGCGTATGGCGTTGACCAATAAAGGCGTGGCTGG
TAGCACACCGCATCAGCTGATTATGACTGCAACGCCCATTCCGCGGACGCTAGCGATGAGTGTTTATGGCGATATGGACA
CTTCTATTATCGATGAGCTACCACCAGGGCGTACACCGATTACTACCGTAACAATTGACCGCAACCGCCGTGATGAGGTG
ATTGAACGTATTGCAGTTAATTGTGAAGCTGGCAGGCAGGCATATTGGGTGTGCTCACTGGTTGAAGAGTCGAGTGTACT
CGATGCGCAAGCTGCTGAGGCGACTTATGAAGATTTGAGCGAGCGTCTGGATATTCGCATTGGCTTGGTGCATGGCAAAA
TGAAAGGCGCTGATAAACAAGCCATTATGCAAGCGTTTAAGGCAGGACAACTTGATTTGTTAATAGCTACTACAGTTATC
GAAGTGGGTGTTGATGTGCCCAATGCTTCTCTGATGGTCATTGAAAATGCAGAGCGATTGGGTCTATCGCAATTGCACCA
ATTACGCGGGCGAGTCGGGCGCGGTTCAACAAAAAGCTATTGCGTGCTGTTGTATCAGAAGCCGTTGTCGGAGACAGGTA
CTGAACGTCTAAATGTATTGCGTGATAGTACTGATGGTTTTGTCATTGCGCAAAAAGATTTAGAGCTGCGTGGTCCGGGC
GAGTTATTAGGAAAGCGTCAAACAGGTAATGTCGGCTATTATTTGGCGGACCTTATTCGGGATGAGCAGTTATTTGCTAT
TGCCCAGCGTTTGGCAAAGCATCTGATTGCAGATCCTGCACGCAAAGCCGATGTCAGCCAACTGATTCATCGTTGGATGC
CAGAGGCAAGTCGTTATACCAATGCTTAA

Upstream 100 bases:

>100_bases
TGATCATTATCACATGATAAGACAGCTTATAAGCTAGCATGAATTCTTGAACAAGACACTTGACCCTGTAAAATTTATAT
TAACATCGTTAGGATTTTTG

Downstream 100 bases:

>100_bases
TGATAGTTAACTCAACCTGTTTTCTATTACTGGTTACCATAAAAAAGCCATTATTTTCTTAAAATAATGGCTTTTTTATG
GTTTTTATAAATCATTTATC

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MPTSVNPATPHLLDVLALDMPVTALAGVGPKVAEQLAQLGIARIFDLLLHLPRDYEDRSRLVSIGDVAHGQAAMITGRVV
HVDTNRSGMTVIVDDDTGTISLRFFKVYRGLAQTMCLGTHLQLFGEVKVSRYGKQIHHPEYQIISSNETVVNTGLQPIYP
SVKGLHQNKLRTLIKLALQTVRSQGLPMTLFTPEDFSVVADLPLVPFEPAKIPITEPDYPEDIFSTLARSVPDNGFSSSD
SSNSSVNRTKPSFYHSQNNTSSDAISMANNNVYNLTIFEALVLLHTPPTYTDAGQQYKLLTQLSARTHAACQRLIIEELT
AHQLSLLYRRQQLHQYKAPKCAIQSPLADKLFGALPFDLTGAQKRVMRDITADMATSIPMLRLVQGDVGAGKTLVAAGAA
GYALDSGWQVAVMAPTEILAEQHLVNFKNWFEPLGIGVGWLAGKQTAKQRREALEAVSENTVQVVVGTHALFQDQVQFAK
LGLVIIDEQHRFGVEQRMALTNKGVAGSTPHQLIMTATPIPRTLAMSVYGDMDTSIIDELPPGRTPITTVTIDRNRRDEV
IERIAVNCEAGRQAYWVCSLVEESSVLDAQAAEATYEDLSERLDIRIGLVHGKMKGADKQAIMQAFKAGQLDLLIATTVI
EVGVDVPNASLMVIENAERLGLSQLHQLRGRVGRGSTKSYCVLLYQKPLSETGTERLNVLRDSTDGFVIAQKDLELRGPG
ELLGKRQTGNVGYYLADLIRDEQLFAIAQRLAKHLIADPARKADVSQLIHRWMPEASRYTNA

Sequences:

>Translated_782_residues
MPTSVNPATPHLLDVLALDMPVTALAGVGPKVAEQLAQLGIARIFDLLLHLPRDYEDRSRLVSIGDVAHGQAAMITGRVV
HVDTNRSGMTVIVDDDTGTISLRFFKVYRGLAQTMCLGTHLQLFGEVKVSRYGKQIHHPEYQIISSNETVVNTGLQPIYP
SVKGLHQNKLRTLIKLALQTVRSQGLPMTLFTPEDFSVVADLPLVPFEPAKIPITEPDYPEDIFSTLARSVPDNGFSSSD
SSNSSVNRTKPSFYHSQNNTSSDAISMANNNVYNLTIFEALVLLHTPPTYTDAGQQYKLLTQLSARTHAACQRLIIEELT
AHQLSLLYRRQQLHQYKAPKCAIQSPLADKLFGALPFDLTGAQKRVMRDITADMATSIPMLRLVQGDVGAGKTLVAAGAA
GYALDSGWQVAVMAPTEILAEQHLVNFKNWFEPLGIGVGWLAGKQTAKQRREALEAVSENTVQVVVGTHALFQDQVQFAK
LGLVIIDEQHRFGVEQRMALTNKGVAGSTPHQLIMTATPIPRTLAMSVYGDMDTSIIDELPPGRTPITTVTIDRNRRDEV
IERIAVNCEAGRQAYWVCSLVEESSVLDAQAAEATYEDLSERLDIRIGLVHGKMKGADKQAIMQAFKAGQLDLLIATTVI
EVGVDVPNASLMVIENAERLGLSQLHQLRGRVGRGSTKSYCVLLYQKPLSETGTERLNVLRDSTDGFVIAQKDLELRGPG
ELLGKRQTGNVGYYLADLIRDEQLFAIAQRLAKHLIADPARKADVSQLIHRWMPEASRYTNA
>Mature_781_residues
PTSVNPATPHLLDVLALDMPVTALAGVGPKVAEQLAQLGIARIFDLLLHLPRDYEDRSRLVSIGDVAHGQAAMITGRVVH
VDTNRSGMTVIVDDDTGTISLRFFKVYRGLAQTMCLGTHLQLFGEVKVSRYGKQIHHPEYQIISSNETVVNTGLQPIYPS
VKGLHQNKLRTLIKLALQTVRSQGLPMTLFTPEDFSVVADLPLVPFEPAKIPITEPDYPEDIFSTLARSVPDNGFSSSDS
SNSSVNRTKPSFYHSQNNTSSDAISMANNNVYNLTIFEALVLLHTPPTYTDAGQQYKLLTQLSARTHAACQRLIIEELTA
HQLSLLYRRQQLHQYKAPKCAIQSPLADKLFGALPFDLTGAQKRVMRDITADMATSIPMLRLVQGDVGAGKTLVAAGAAG
YALDSGWQVAVMAPTEILAEQHLVNFKNWFEPLGIGVGWLAGKQTAKQRREALEAVSENTVQVVVGTHALFQDQVQFAKL
GLVIIDEQHRFGVEQRMALTNKGVAGSTPHQLIMTATPIPRTLAMSVYGDMDTSIIDELPPGRTPITTVTIDRNRRDEVI
ERIAVNCEAGRQAYWVCSLVEESSVLDAQAAEATYEDLSERLDIRIGLVHGKMKGADKQAIMQAFKAGQLDLLIATTVIE
VGVDVPNASLMVIENAERLGLSQLHQLRGRVGRGSTKSYCVLLYQKPLSETGTERLNVLRDSTDGFVIAQKDLELRGPGE
LLGKRQTGNVGYYLADLIRDEQLFAIAQRLAKHLIADPARKADVSQLIHRWMPEASRYTNA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=511, Percent_Identity=61.0567514677104, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787357, Length=392, Percent_Identity=38.265306122449, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85899; Mature: 85767

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSVNPATPHLLDVLALDMPVTALAGVGPKVAEQLAQLGIARIFDLLLHLPRDYEDRSR
CCCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LVSIGDVAHGQAAMITGRVVHVDTNRSGMTVIVDDDTGTISLRFFKVYRGLAQTMCLGTH
EEEECCCCCCCEEEEEEEEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
LQLFGEVKVSRYGKQIHHPEYQIISSNETVVNTGLQPIYPSVKGLHQNKLRTLIKLALQT
HHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
VRSQGLPMTLFTPEDFSVVADLPLVPFEPAKIPITEPDYPEDIFSTLARSVPDNGFSSSD
HHHCCCCEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
SSNSSVNRTKPSFYHSQNNTSSDAISMANNNVYNLTIFEALVLLHTPPTYTDAGQQYKLL
CCCCCCCCCCCCEEECCCCCCCHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHH
TQLSARTHAACQRLIIEELTAHQLSLLYRRQQLHQYKAPKCAIQSPLADKLFGALPFDLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCCCC
GAQKRVMRDITADMATSIPMLRLVQGDVGAGKTLVAAGAAGYALDSGWQVAVMAPTEILA
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCCEECCCCEEEEECHHHHHH
EQHLVNFKNWFEPLGIGVGWLAGKQTAKQRREALEAVSENTVQVVVGTHALFQDQVQFAK
HHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHH
LGLVIIDEQHRFGVEQRMALTNKGVAGSTPHQLIMTATPIPRTLAMSVYGDMDTSIIDEL
CCEEEEECHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHC
PPGRTPITTVTIDRNRRDEVIERIAVNCEAGRQAYWVCSLVEESSVLDAQAAEATYEDLS
CCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
ERLDIRIGLVHGKMKGADKQAIMQAFKAGQLDLLIATTVIEVGVDVPNASLMVIENAERL
HHCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEECCHHHC
GLSQLHQLRGRVGRGSTKSYCVLLYQKPLSETGTERLNVLRDSTDGFVIAQKDLELRGPG
CHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCH
ELLGKRQTGNVGYYLADLIRDEQLFAIAQRLAKHLIADPARKADVSQLIHRWMPEASRYT
HHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHCC
NA
CC
>Mature Secondary Structure 
PTSVNPATPHLLDVLALDMPVTALAGVGPKVAEQLAQLGIARIFDLLLHLPRDYEDRSR
CCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LVSIGDVAHGQAAMITGRVVHVDTNRSGMTVIVDDDTGTISLRFFKVYRGLAQTMCLGTH
EEEECCCCCCCEEEEEEEEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
LQLFGEVKVSRYGKQIHHPEYQIISSNETVVNTGLQPIYPSVKGLHQNKLRTLIKLALQT
HHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
VRSQGLPMTLFTPEDFSVVADLPLVPFEPAKIPITEPDYPEDIFSTLARSVPDNGFSSSD
HHHCCCCEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
SSNSSVNRTKPSFYHSQNNTSSDAISMANNNVYNLTIFEALVLLHTPPTYTDAGQQYKLL
CCCCCCCCCCCCEEECCCCCCCHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHH
TQLSARTHAACQRLIIEELTAHQLSLLYRRQQLHQYKAPKCAIQSPLADKLFGALPFDLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCCCCCC
GAQKRVMRDITADMATSIPMLRLVQGDVGAGKTLVAAGAAGYALDSGWQVAVMAPTEILA
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCCCCEECCCCEEEEECHHHHHH
EQHLVNFKNWFEPLGIGVGWLAGKQTAKQRREALEAVSENTVQVVVGTHALFQDQVQFAK
HHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHH
LGLVIIDEQHRFGVEQRMALTNKGVAGSTPHQLIMTATPIPRTLAMSVYGDMDTSIIDEL
CCEEEEECHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHC
PPGRTPITTVTIDRNRRDEVIERIAVNCEAGRQAYWVCSLVEESSVLDAQAAEATYEDLS
CCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
ERLDIRIGLVHGKMKGADKQAIMQAFKAGQLDLLIATTVIEVGVDVPNASLMVIENAERL
HHCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEECCHHHC
GLSQLHQLRGRVGRGSTKSYCVLLYQKPLSETGTERLNVLRDSTDGFVIAQKDLELRGPG
CHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCH
ELLGKRQTGNVGYYLADLIRDEQLFAIAQRLAKHLIADPARKADVSQLIHRWMPEASRYT
HHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHCC
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]