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The map label for this gene is yafJ [C]

Identifier: 93007212

GI number: 93007212

Start: 2937437

End: 2938360

Strand: Reverse

Name: yafJ [C]

Synonym: Pcryo_2388

Alternate gene names: 93007212

Gene position: 2938360-2937437 (Counterclockwise)

Preceding gene: 93007213

Following gene: 93007209

Centisome position: 96.03

GC content: 43.51

Gene sequence:

>924_bases
ATGTGTCAACTTTTAGGTATGAACTGTAATACGCCAACCGATATTGGTTTTAGCTTTGCAGGCTTCCGTCGTCGCGGCGG
CATGACAGACAGTCATGAGGATGGTTTTGGTATTGCTTTTTTTGAGCGTAGCGAATGCACTAATAGTGACAGCTCTAATA
GTGAAAAGTCGGACAATGCCTCGACTGGTCTACGCTTATTCCATGACAATCGCCCAAGCCATTTATCACCCGTTGCTGAT
TTGGTTAATAATTATCCAATTAAAGCGATGAATGTTATCGCACATATTCGTAAAGCCACCCAAGGTCAGAATTGTTTGGC
GAATACCCATCCTTTTGTCCGTGAAGTCTGGGGCGAGCAGTGGGTATTTGCGCACAACGGACAGATGAATAGCGAATTTA
TTAAACGCTGCCAGCGCCTGCAAGACAATGGTAATGCCTCACATTGCCAACCAGTCGGCACAACTGATTCTGAAATGGCA
TTCTGTTATCTGGTCAATCGGCTGAAAAGTAGCTTTAAAACTCGTCCGGATGACAAAACGCTGTTTAACTTTTTGACGAC
TCAATGTCGTTATTTATCCGCTAACGGTTTGTTTAATTGCTTGATATCGAATGGTCAATGGCAATTGGCTTATGCAGGCA
GTTTACTGTTTTATCTCACGCGCAAAGCACCATTTGGTGAAGCGAAATTAGCAGATGATGATTTGGCGATTAACTTTGGT
GATGTGACCACTGATAAAGATAAAGTGACTATCTTAGTGACTGTGCCATTGACTGAAAATGAAAAATGGCAACAGTTGGC
AGTCAATGAGTGCTTAATATTTCAAGATGGGGATGTCATTTATAAAGACAGCCCAAGCCAGCGCAAGTTTTTGACGATTG
AGGAAGGCGTTGCGGTAGCACGGGCAGTTGGGGCGAGTGTTTAG

Upstream 100 bases:

>100_bases
AGATACCGATTTGGTGCATGCCAAACGCATTTTGGATAGTATTGATAAGGATGCGGCAAGCTTGCCTGAAACAGACGACG
ATAACGGCGGTACTCTATAA

Downstream 100 bases:

>100_bases
GAGTGGTGAATAGTGGATGTTAATTAAATATACATTCCACAACAACGCTTAAATTTCTCACCCGAACCGCAAATACACGG
CTGTTTTTGGGTTATAGTCA

Product: glutamine amidotransferase, class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MCQLLGMNCNTPTDIGFSFAGFRRRGGMTDSHEDGFGIAFFERSECTNSDSSNSEKSDNASTGLRLFHDNRPSHLSPVAD
LVNNYPIKAMNVIAHIRKATQGQNCLANTHPFVREVWGEQWVFAHNGQMNSEFIKRCQRLQDNGNASHCQPVGTTDSEMA
FCYLVNRLKSSFKTRPDDKTLFNFLTTQCRYLSANGLFNCLISNGQWQLAYAGSLLFYLTRKAPFGEAKLADDDLAINFG
DVTTDKDKVTILVTVPLTENEKWQQLAVNECLIFQDGDVIYKDSPSQRKFLTIEEGVAVARAVGASV

Sequences:

>Translated_307_residues
MCQLLGMNCNTPTDIGFSFAGFRRRGGMTDSHEDGFGIAFFERSECTNSDSSNSEKSDNASTGLRLFHDNRPSHLSPVAD
LVNNYPIKAMNVIAHIRKATQGQNCLANTHPFVREVWGEQWVFAHNGQMNSEFIKRCQRLQDNGNASHCQPVGTTDSEMA
FCYLVNRLKSSFKTRPDDKTLFNFLTTQCRYLSANGLFNCLISNGQWQLAYAGSLLFYLTRKAPFGEAKLADDDLAINFG
DVTTDKDKVTILVTVPLTENEKWQQLAVNECLIFQDGDVIYKDSPSQRKFLTIEEGVAVARAVGASV
>Mature_307_residues
MCQLLGMNCNTPTDIGFSFAGFRRRGGMTDSHEDGFGIAFFERSECTNSDSSNSEKSDNASTGLRLFHDNRPSHLSPVAD
LVNNYPIKAMNVIAHIRKATQGQNCLANTHPFVREVWGEQWVFAHNGQMNSEFIKRCQRLQDNGNASHCQPVGTTDSEMA
FCYLVNRLKSSFKTRPDDKTLFNFLTTQCRYLSANGLFNCLISNGQWQLAYAGSLLFYLTRKAPFGEAKLADDDLAINFG
DVTTDKDKVTILVTVPLTENEKWQQLAVNECLIFQDGDVIYKDSPSQRKFLTIEEGVAVARAVGASV

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=281, Percent_Identity=41.9928825622776, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 34240; Mature: 34240

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCNTPTDIGFSFAGFRRRGGMTDSHEDGFGIAFFERSECTNSDSSNSEKSDNA
CCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
STGLRLFHDNRPSHLSPVADLVNNYPIKAMNVIAHIRKATQGQNCLANTHPFVREVWGEQ
CCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
WVFAHNGQMNSEFIKRCQRLQDNGNASHCQPVGTTDSEMAFCYLVNRLKSSFKTRPDDKT
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LFNFLTTQCRYLSANGLFNCLISNGQWQLAYAGSLLFYLTRKAPFGEAKLADDDLAINFG
HHHHHHHHHHEECCCCEEEEEECCCCEEEEECCEEEEEEECCCCCCCCEECCCCEEEEEC
DVTTDKDKVTILVTVPLTENEKWQQLAVNECLIFQDGDVIYKDSPSQRKFLTIEEGVAVA
CCCCCCCCEEEEEEEECCCCCHHHHHHHCEEEEEECCCEEECCCCCCCEEEEHHHHHHHH
RAVGASV
HHHCCCC
>Mature Secondary Structure
MCQLLGMNCNTPTDIGFSFAGFRRRGGMTDSHEDGFGIAFFERSECTNSDSSNSEKSDNA
CCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
STGLRLFHDNRPSHLSPVADLVNNYPIKAMNVIAHIRKATQGQNCLANTHPFVREVWGEQ
CCCEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
WVFAHNGQMNSEFIKRCQRLQDNGNASHCQPVGTTDSEMAFCYLVNRLKSSFKTRPDDKT
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
LFNFLTTQCRYLSANGLFNCLISNGQWQLAYAGSLLFYLTRKAPFGEAKLADDDLAINFG
HHHHHHHHHHEECCCCEEEEEECCCCEEEEECCEEEEEEECCCCCCCCEECCCCEEEEEC
DVTTDKDKVTILVTVPLTENEKWQQLAVNECLIFQDGDVIYKDSPSQRKFLTIEEGVAVA
CCCCCCCCEEEEEEEECCCCCHHHHHHHCEEEEEECCCEEECCCCCCCEEEEHHHHHHHH
RAVGASV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]