Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 93007117

GI number: 93007117

Start: 2820537

End: 2821844

Strand: Direct

Name: rho [H]

Synonym: Pcryo_2293

Alternate gene names: 93007117

Gene position: 2820537-2821844 (Clockwise)

Preceding gene: 93007116

Following gene: 93007118

Centisome position: 92.18

GC content: 44.88

Gene sequence:

>1308_bases
ATGGCATCTCTCATCTTATCAACTGCTAACGACCTATCTATGAATCTTACTGAATTAAAGAAAAAATCAATCGCTGAGCT
ACTGGCCATCGCTAAAGAGATGGGTCTTGATAATATGGCACGTAGCCGTAAACAAGACATTATCTTTGCCATTCTAAAAA
CTCATGCCCGTAACGGTGAGGCCATCTATGGTGATGGTGTGCTTGAAGTGCTCCCTGACGGTTTTGGTTTTTTGCGCTCA
TCTGAAGGCTCTTATTTAGCAGGTCCTGATGATATCTATGTCAGCCCAAGCCAAATTCGCCGTTTCAGTCTAAAAACTGG
CGACAGTATCGCAGGCACGATTCGTCCACCAAAAGACTCTGAGCGTTATTTTGCACTATTAAAAGTCGGTGAAATCAACT
TTGATACGCCAGACCGCTCACGTCATAAGCTTATCTTTGAAAACTTAACGCCATTATTTCCAACTGAGCAGCTAAAACTT
GAGCTAGGTAACGGTACGACTGAAGATTTGACTGGTCGTATTATCGACCTGATCGCACCGATTGGTAAAGGTCAGCGCTC
TATTATTGTGGCACCGCCCAAAGCGGGTAAAACGATGCTGCTACAGTCAATAGCACAATCGATCACTCGTAATAATCCTG
AATGCTACCTCATTGTGCTGCTGATTGATGAGCGTCCAGAAGAAGTCACCGAGATGGAACGTACTGTACGCGGTGAAGTG
GTTGCCTCAACTTTCGATGAGCCGCCACAGCGCCATGTACAAGTGGCTGAAATGGTCATTGAAAAAGCCAAGCGCTTGGT
TGAGCACAAGCAAGACGTGGTCATTCTGCTTGACTCGATTACCCGTCTTGCCCGCGCCTATAACACCGTTATTCCTTCAT
CAGGTAAAGTGTTGACCGGTGGTTTGGATGCCAATGCGTTAGAGCGCCCAAAACGATTCTTTGGTGCTGCTCGTAATATT
GAAGAAGGCGGCAGCTTGACCATTATCGCCAGTGCCCTTATTGATACTGGTAGTAAAATGGACAGCGTTATCTTTGAAGA
ATTCAAAGGTACCGGTAACCAAGAGATTACCCTTGAGCGTGATCTGGCAGAAAAGCGTGTCTTCCCTGCCATTAATATCA
AAAAATCGGGTACACGCCGTGAAGAACGTCTGCTTGATGAAGATAAGCTGCGTAAAGTTTGGATCCTGCGTAAATTGCTA
CAGCCGATGGATGGTGTACAAGCAACTGAGTTCTTATTGGACCGCTTAAAAGAAGCCAAAACCAATGATGAATTTTTTGA
GCAAATGAAGCGTAAATCACAAAATTGA

Upstream 100 bases:

>100_bases
ACTGACACATAAAATCAAAACTACATAAATTTTAACTCTGTATATATACCCTCTATCGCCCTAATAACATTGTTTTAATC
ACTGTCGTGATTTATGAATA

Downstream 100 bases:

>100_bases
TTTTAAATCTTTTACGACAATAGAGTTATATAAATAATTGACGTTTTACTCATAAAGGCTGCCTATAAGGCGGTCTTTTT
GTTTTTATTACTATTGGCTC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MASLILSTANDLSMNLTELKKKSIAELLAIAKEMGLDNMARSRKQDIIFAILKTHARNGEAIYGDGVLEVLPDGFGFLRS
SEGSYLAGPDDIYVSPSQIRRFSLKTGDSIAGTIRPPKDSERYFALLKVGEINFDTPDRSRHKLIFENLTPLFPTEQLKL
ELGNGTTEDLTGRIIDLIAPIGKGQRSIIVAPPKAGKTMLLQSIAQSITRNNPECYLIVLLIDERPEEVTEMERTVRGEV
VASTFDEPPQRHVQVAEMVIEKAKRLVEHKQDVVILLDSITRLARAYNTVIPSSGKVLTGGLDANALERPKRFFGAARNI
EEGGSLTIIASALIDTGSKMDSVIFEEFKGTGNQEITLERDLAEKRVFPAINIKKSGTRREERLLDEDKLRKVWILRKLL
QPMDGVQATEFLLDRLKEAKTNDEFFEQMKRKSQN

Sequences:

>Translated_435_residues
MASLILSTANDLSMNLTELKKKSIAELLAIAKEMGLDNMARSRKQDIIFAILKTHARNGEAIYGDGVLEVLPDGFGFLRS
SEGSYLAGPDDIYVSPSQIRRFSLKTGDSIAGTIRPPKDSERYFALLKVGEINFDTPDRSRHKLIFENLTPLFPTEQLKL
ELGNGTTEDLTGRIIDLIAPIGKGQRSIIVAPPKAGKTMLLQSIAQSITRNNPECYLIVLLIDERPEEVTEMERTVRGEV
VASTFDEPPQRHVQVAEMVIEKAKRLVEHKQDVVILLDSITRLARAYNTVIPSSGKVLTGGLDANALERPKRFFGAARNI
EEGGSLTIIASALIDTGSKMDSVIFEEFKGTGNQEITLERDLAEKRVFPAINIKKSGTRREERLLDEDKLRKVWILRKLL
QPMDGVQATEFLLDRLKEAKTNDEFFEQMKRKSQN
>Mature_434_residues
ASLILSTANDLSMNLTELKKKSIAELLAIAKEMGLDNMARSRKQDIIFAILKTHARNGEAIYGDGVLEVLPDGFGFLRSS
EGSYLAGPDDIYVSPSQIRRFSLKTGDSIAGTIRPPKDSERYFALLKVGEINFDTPDRSRHKLIFENLTPLFPTEQLKLE
LGNGTTEDLTGRIIDLIAPIGKGQRSIIVAPPKAGKTMLLQSIAQSITRNNPECYLIVLLIDERPEEVTEMERTVRGEVV
ASTFDEPPQRHVQVAEMVIEKAKRLVEHKQDVVILLDSITRLARAYNTVIPSSGKVLTGGLDANALERPKRFFGAARNIE
EGGSLTIIASALIDTGSKMDSVIFEEFKGTGNQEITLERDLAEKRVFPAINIKKSGTRREERLLDEDKLRKVWILRKLLQ
PMDGVQATEFLLDRLKEAKTNDEFFEQMKRKSQN

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=72.7272727272727, Blast_Score=651, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48622; Mature: 48490

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLILSTANDLSMNLTELKKKSIAELLAIAKEMGLDNMARSRKQDIIFAILKTHARNGE
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
AIYGDGVLEVLPDGFGFLRSSEGSYLAGPDDIYVSPSQIRRFSLKTGDSIAGTIRPPKDS
EEECCCHHHHCCCCHHHHCCCCCCEECCCCCEEECHHHHHEECCCCCCCCEECCCCCCCC
ERYFALLKVGEINFDTPDRSRHKLIFENLTPLFPTEQLKLELGNGTTEDLTGRIIDLIAP
CCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHC
IGKGQRSIIVAPPKAGKTMLLQSIAQSITRNNPECYLIVLLIDERPEEVTEMERTVRGEV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH
VASTFDEPPQRHVQVAEMVIEKAKRLVEHKQDVVILLDSITRLARAYNTVIPSSGKVLTG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEC
GLDANALERPKRFFGAARNIEEGGSLTIIASALIDTGSKMDSVIFEEFKGTGNQEITLER
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEH
DLAEKRVFPAINIKKSGTRREERLLDEDKLRKVWILRKLLQPMDGVQATEFLLDRLKEAK
HHHHHHCCCCEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
TNDEFFEQMKRKSQN
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
ASLILSTANDLSMNLTELKKKSIAELLAIAKEMGLDNMARSRKQDIIFAILKTHARNGE
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
AIYGDGVLEVLPDGFGFLRSSEGSYLAGPDDIYVSPSQIRRFSLKTGDSIAGTIRPPKDS
EEECCCHHHHCCCCHHHHCCCCCCEECCCCCEEECHHHHHEECCCCCCCCEECCCCCCCC
ERYFALLKVGEINFDTPDRSRHKLIFENLTPLFPTEQLKLELGNGTTEDLTGRIIDLIAP
CCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHC
IGKGQRSIIVAPPKAGKTMLLQSIAQSITRNNPECYLIVLLIDERPEEVTEMERTVRGEV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH
VASTFDEPPQRHVQVAEMVIEKAKRLVEHKQDVVILLDSITRLARAYNTVIPSSGKVLTG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEC
GLDANALERPKRFFGAARNIEEGGSLTIIASALIDTGSKMDSVIFEEFKGTGNQEITLER
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEH
DLAEKRVFPAINIKKSGTRREERLLDEDKLRKVWILRKLLQPMDGVQATEFLLDRLKEAK
HHHHHHCCCCEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
TNDEFFEQMKRKSQN
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]