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The map label for this gene is phoB [H]

Identifier: 93007040

GI number: 93007040

Start: 2721017

End: 2721721

Strand: Reverse

Name: phoB [H]

Synonym: Pcryo_2216

Alternate gene names: 93007040

Gene position: 2721721-2721017 (Counterclockwise)

Preceding gene: 93007041

Following gene: 93007039

Centisome position: 88.95

GC content: 45.67

Gene sequence:

>705_bases
ATGTCTAAAGAGCAAATTTTGATTGTTGAAGATGAAGATGCCATTCGTGAAATGGTCGTCATGACCCTAGAGATGGCGGG
CTTTGATAGTTTGCAGGCAGCGGATGTGTCAGAGGCGCACCAACAAGTGGTCGATCATCGTCCAGCGCTGATTTTATTGG
ACTGGATGTTACCCGGTGATAAAAGTGGTATCGATTTTTGCCGTATGCTAAAAAGCGATACGCTGCTCGCCGAGATACCG
GTTATTATGTTGACCGCCAAAAGTGAAGAAGACAGTAAAGTGCATGGTCTTGACGCAGGCGCTGATGATTATATGACAAA
GCCTTTCTCGACCCGTGAGCTTATCTCAAGAATCAAAGCGGTATTGCGACGTAGTAACGCGCTAAGCGGCGACAAACCTA
TTAATATAGGCAGGCTAACTTTAGATGCCAAAAGTCAACGCTTCATGGTGGGTGATGATATTATCGATATGGGTCCGACA
GAATATCGCTTATTGGCGTTTTTTATGAGCCATCCTGAGCGCGCTTATACCCGTGCCCAGCTGCTCGATCAAGTATGGGG
CGGTAACGTCTACATCGAAGATAGAACCATTGATGTTCACGTAAAGCGTTTGCGCACATTACTACGTCCACATGATTGTG
ATACATTGATACAAACGGTACGCGGTACAGGTTATCGATTTTCTAGCCGTATCGAAAAAGGGTAA

Upstream 100 bases:

>100_bases
TAATTAAGCCCTTTAATGAAGAAACGCTTATTTGCGTTTGTTACTGCTTTTATAAACGCTATTTTGTCCTCTAACATTAA
GAATATATTAAGAATACATT

Downstream 100 bases:

>100_bases
TCGCTAGTAATATGAATATTGTATAGTGTCACCTTTTAATAGTGTCGCCTTTTATCGGGAGCGGCCAATAGTAGAAAAAT
TATAAAAAATAATGGATAAA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSKEQILIVEDEDAIREMVVMTLEMAGFDSLQAADVSEAHQQVVDHRPALILLDWMLPGDKSGIDFCRMLKSDTLLAEIP
VIMLTAKSEEDSKVHGLDAGADDYMTKPFSTRELISRIKAVLRRSNALSGDKPINIGRLTLDAKSQRFMVGDDIIDMGPT
EYRLLAFFMSHPERAYTRAQLLDQVWGGNVYIEDRTIDVHVKRLRTLLRPHDCDTLIQTVRGTGYRFSSRIEKG

Sequences:

>Translated_234_residues
MSKEQILIVEDEDAIREMVVMTLEMAGFDSLQAADVSEAHQQVVDHRPALILLDWMLPGDKSGIDFCRMLKSDTLLAEIP
VIMLTAKSEEDSKVHGLDAGADDYMTKPFSTRELISRIKAVLRRSNALSGDKPINIGRLTLDAKSQRFMVGDDIIDMGPT
EYRLLAFFMSHPERAYTRAQLLDQVWGGNVYIEDRTIDVHVKRLRTLLRPHDCDTLIQTVRGTGYRFSSRIEKG
>Mature_233_residues
SKEQILIVEDEDAIREMVVMTLEMAGFDSLQAADVSEAHQQVVDHRPALILLDWMLPGDKSGIDFCRMLKSDTLLAEIPV
IMLTAKSEEDSKVHGLDAGADDYMTKPFSTRELISRIKAVLRRSNALSGDKPINIGRLTLDAKSQRFMVGDDIIDMGPTE
YRLLAFFMSHPERAYTRAQLLDQVWGGNVYIEDRTIDVHVKRLRTLLRPHDCDTLIQTVRGTGYRFSSRIEKG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=55.0660792951542, Blast_Score=266, Evalue=6e-73,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=38.0952380952381, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=36.7256637168142, Blast_Score=136, Evalue=9e-34,
Organism=Escherichia coli, GI1788394, Length=234, Percent_Identity=34.1880341880342, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=34.3478260869565, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=36.1990950226244, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790863, Length=249, Percent_Identity=31.7269076305221, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=33.6322869955157, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787375, Length=226, Percent_Identity=30.0884955752212, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=35.3711790393013, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790552, Length=232, Percent_Identity=32.7586206896552, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=26.5486725663717, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=28.448275862069, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=26.3829787234043, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=34.5454545454545, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26482; Mature: 26351

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEQILIVEDEDAIREMVVMTLEMAGFDSLQAADVSEAHQQVVDHRPALILLDWMLPGD
CCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCC
KSGIDFCRMLKSDTLLAEIPVIMLTAKSEEDSKVHGLDAGADDYMTKPFSTRELISRIKA
CCHHHHHHHHHCCCCEEECCEEEEEECCCCCCCEECCCCCCCHHCCCCCCHHHHHHHHHH
VLRRSNALSGDKPINIGRLTLDAKSQRFMVGDDIIDMGPTEYRLLAFFMSHPERAYTRAQ
HHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LLDQVWGGNVYIEDRTIDVHVKRLRTLLRPHDCDTLIQTVRGTGYRFSSRIEKG
HHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCCC
>Mature Secondary Structure 
SKEQILIVEDEDAIREMVVMTLEMAGFDSLQAADVSEAHQQVVDHRPALILLDWMLPGD
CCCCEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCC
KSGIDFCRMLKSDTLLAEIPVIMLTAKSEEDSKVHGLDAGADDYMTKPFSTRELISRIKA
CCHHHHHHHHHCCCCEEECCEEEEEECCCCCCCEECCCCCCCHHCCCCCCHHHHHHHHHH
VLRRSNALSGDKPINIGRLTLDAKSQRFMVGDDIIDMGPTEYRLLAFFMSHPERAYTRAQ
HHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LLDQVWGGNVYIEDRTIDVHVKRLRTLLRPHDCDTLIQTVRGTGYRFSSRIEKG
HHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]