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The map label for this gene is fusA [H]

Identifier: 93007009

GI number: 93007009

Start: 2685351

End: 2687477

Strand: Reverse

Name: fusA [H]

Synonym: Pcryo_2185

Alternate gene names: 93007009

Gene position: 2687477-2685351 (Counterclockwise)

Preceding gene: 93007010

Following gene: 93007008

Centisome position: 87.83

GC content: 44.01

Gene sequence:

>2127_bases
ATGGCTCGTAAAACTCCCCTAAAGCGCTATCGCAATATTGGTATCTCTGCGCACATTGATGCTGGTAAAACGACCACTAC
TGAACGTGTTTTGTTCTATACCGGTGTTAGCCACAAGATTGGTGAAGTACATGATGGCGCAGCCACTATGGACTGGATGG
AGCAAGAACAAGAGCGTGGTATTACTATCACCTCAGCGGCGACAACTTGTTTTTGGTCAGGTATGGCAAAACAGTTTGAC
GAACACCGTATCAATATCATTGACACCCCAGGTCACGTTGACTTCACAATTGAAGTTGAACGTTCTATGCGTGTTCTTGA
TGGCGCTTGCATGGTTTACTGTGCAGTAGGCGGCGTTCAGCCACAGTCTGAAACCGTATGGCGTCAGGCAAACAAATATA
AAGTTCCACGTCTTGCATTCATTAACAAAATGGATCGCGTTGGCGCTGATTTCTACCGCGTAGTAGAACAGATCAAAACT
CGTCTAGGCGGCAAGCCAGTACCATTGGTTATCCCAATTGGTAAAGAAGACGACTTTGAAGGCGTTGTAGATCTAGTAAC
GATGAAAGCCATCTATTGGGACGAAGCGTCTCAAGGCATGGAATATGATGAGCGTGAAATCCCAGCAGAACTACAAGAAA
AAGCGGAAGAATATCGTGAGTATCTAGTAGAAAGCGCTGCTGAAGCGACTGAAGAATTGATGAATGAGTATCTTGAGAAC
GGTGAATTAACTGTTGATCAAATTAACTCTGCTATTCGCCAGTTGACTATTGATAACGAAATCATTCCACTTCTTTGTGG
TACTGCCTTTAAAAACAAAGGTGTACAGAAGATGTTGGACGCTGTTATTCAGTATCTTCCTGCACCAATGGATGTACCTG
CTATTAAAGGTATCCTGGATGACAAAGATGAATCTGAAGGCACTCGTGAAGCGTCAGACGAAGCACCGTTTTCAGCTTTA
GCATTCAAAATCATGAATGATAAGTTCGTTGGTAACTTAACCTTCGTTCGTGTTTATTCAGGTGTTCTAAAGCAGGGCAG
CAGCGTTTATAACCCAGTTAAAATGAAACGTGAGCGTGTTGGCCGTATCGTACAGATGATGGCAAACTCTCAAGAAGAGT
TGCAAGAAATCCGTACTGGTGATATCGCAGCCTTGGTTGGCATGAAAGATGTAACCACTGGTGATACGTTGTGTGACGAG
CAAAATGTTATTACGCTTGAGCGTATGGAATTCCCAGATCCAGTTATCAGTCTAGCGGTTGAACCTAAAACCAAAGCTGA
CCAAGAAAAAATGTCAATCGCTTTAGGTCGTTTGGCGAAAGAAGATCCATCGTTCCGTGTACATACTGACGAAGAATCTG
GTCAGACTATCATCAGTGGTATGGGTGAGCTGCATCTTGAGATTCTTGTTGACCGTATGAAGCGTGAGTTTGGTGTTGAA
GCTAACATCGGCGCACCTCAGGTTGCTTATCGCGAAACGATTCGTAACACGGTTGAGCAAGAAGGCAAATTCGTACGTCA
GACTGGTGGTCGTGGTAAATTCGGTCACGTTTGGTTACGTCTTGAGCCTATGGATCCAGCTGGCGATGTTCTTTATGAAT
TTAAAGAAGAAGTTGTTGGTGGTACGGTACCAAAAGAATTCCACGGTGCGGTTGATAAAGGTATTCAAGAGCGCATGAAA
AACGGCGTACTTGCAGGTTACCCAATCGTTGGCGTAAAAGCGACCTTGTATGATGGTTCTTACCATGATGTTGACTCGGA
TGAGTTATCATTTAAAATGGCTGGTTCTATCGCATTCCGTAAAGGCTTCATGGCTGCAAACCCAACGCTACTTGAGCCAG
TGATGAAAGTAGAAGTTGAAACACCTGAAGACTATATGGGCGATATCATGGGCGATCTTAACCGTCGTCGTGGTATGGTT
CAAGGTATGGAAGACTTACCTGGCGGCACTAAGCAGATTCGTGCTGAAGTACCATTAGCGGAAATGTTTGGCTATGCCAC
ACAAATGCGTTCAATGTCACAGGGCCGTGCAACTTACTCTATGGAATTCCAAAAGTACGCTGAAATTCCAAAATCAGTTG
CAGAAGCTATCATCTCTAAATTCAACAATAAAGATGATGACGAATAA

Upstream 100 bases:

>100_bases
TCAATGATTAGTAATATTGCAGGGCTGAACGGCTTTGTCATAGAGTAGAGGTAGAGACACAACACATTAATATATACACG
AATTTCCCTAGGAAAACACT

Downstream 100 bases:

>100_bases
GCAAAAATCTATCGATAATGGCAATATAGGACATATCTCTATATTGTCAGTAGATAATCGAGCAATAATTGGTTAAAAGA
CTTGTTATTGCTCGTGATTT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MARKTPLKRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWSGMAKQFD
EHRINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFINKMDRVGADFYRVVEQIKT
RLGGKPVPLVIPIGKEDDFEGVVDLVTMKAIYWDEASQGMEYDEREIPAELQEKAEEYREYLVESAAEATEELMNEYLEN
GELTVDQINSAIRQLTIDNEIIPLLCGTAFKNKGVQKMLDAVIQYLPAPMDVPAIKGILDDKDESEGTREASDEAPFSAL
AFKIMNDKFVGNLTFVRVYSGVLKQGSSVYNPVKMKRERVGRIVQMMANSQEELQEIRTGDIAALVGMKDVTTGDTLCDE
QNVITLERMEFPDPVISLAVEPKTKADQEKMSIALGRLAKEDPSFRVHTDEESGQTIISGMGELHLEILVDRMKREFGVE
ANIGAPQVAYRETIRNTVEQEGKFVRQTGGRGKFGHVWLRLEPMDPAGDVLYEFKEEVVGGTVPKEFHGAVDKGIQERMK
NGVLAGYPIVGVKATLYDGSYHDVDSDELSFKMAGSIAFRKGFMAANPTLLEPVMKVEVETPEDYMGDIMGDLNRRRGMV
QGMEDLPGGTKQIRAEVPLAEMFGYATQMRSMSQGRATYSMEFQKYAEIPKSVAEAIISKFNNKDDDE

Sequences:

>Translated_708_residues
MARKTPLKRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWSGMAKQFD
EHRINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFINKMDRVGADFYRVVEQIKT
RLGGKPVPLVIPIGKEDDFEGVVDLVTMKAIYWDEASQGMEYDEREIPAELQEKAEEYREYLVESAAEATEELMNEYLEN
GELTVDQINSAIRQLTIDNEIIPLLCGTAFKNKGVQKMLDAVIQYLPAPMDVPAIKGILDDKDESEGTREASDEAPFSAL
AFKIMNDKFVGNLTFVRVYSGVLKQGSSVYNPVKMKRERVGRIVQMMANSQEELQEIRTGDIAALVGMKDVTTGDTLCDE
QNVITLERMEFPDPVISLAVEPKTKADQEKMSIALGRLAKEDPSFRVHTDEESGQTIISGMGELHLEILVDRMKREFGVE
ANIGAPQVAYRETIRNTVEQEGKFVRQTGGRGKFGHVWLRLEPMDPAGDVLYEFKEEVVGGTVPKEFHGAVDKGIQERMK
NGVLAGYPIVGVKATLYDGSYHDVDSDELSFKMAGSIAFRKGFMAANPTLLEPVMKVEVETPEDYMGDIMGDLNRRRGMV
QGMEDLPGGTKQIRAEVPLAEMFGYATQMRSMSQGRATYSMEFQKYAEIPKSVAEAIISKFNNKDDDE
>Mature_707_residues
ARKTPLKRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWSGMAKQFDE
HRINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFINKMDRVGADFYRVVEQIKTR
LGGKPVPLVIPIGKEDDFEGVVDLVTMKAIYWDEASQGMEYDEREIPAELQEKAEEYREYLVESAAEATEELMNEYLENG
ELTVDQINSAIRQLTIDNEIIPLLCGTAFKNKGVQKMLDAVIQYLPAPMDVPAIKGILDDKDESEGTREASDEAPFSALA
FKIMNDKFVGNLTFVRVYSGVLKQGSSVYNPVKMKRERVGRIVQMMANSQEELQEIRTGDIAALVGMKDVTTGDTLCDEQ
NVITLERMEFPDPVISLAVEPKTKADQEKMSIALGRLAKEDPSFRVHTDEESGQTIISGMGELHLEILVDRMKREFGVEA
NIGAPQVAYRETIRNTVEQEGKFVRQTGGRGKFGHVWLRLEPMDPAGDVLYEFKEEVVGGTVPKEFHGAVDKGIQERMKN
GVLAGYPIVGVKATLYDGSYHDVDSDELSFKMAGSIAFRKGFMAANPTLLEPVMKVEVETPEDYMGDIMGDLNRRRGMVQ
GMEDLPGGTKQIRAEVPLAEMFGYATQMRSMSQGRATYSMEFQKYAEIPKSVAEAIISKFNNKDDDE

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=706, Percent_Identity=44.0509915014164, Blast_Score=585, Evalue=1e-167,
Organism=Homo sapiens, GI19923640, Length=718, Percent_Identity=37.7437325905292, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI25306287, Length=716, Percent_Identity=34.3575418994413, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI25306283, Length=457, Percent_Identity=39.1684901531729, Blast_Score=317, Evalue=3e-86,
Organism=Homo sapiens, GI4503483, Length=484, Percent_Identity=26.8595041322314, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157426893, Length=153, Percent_Identity=36.6013071895425, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=41.0071942446043, Blast_Score=97, Evalue=8e-20,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=40.8333333333333, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=40.8333333333333, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=40.8333333333333, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1789738, Length=700, Percent_Identity=69.7142857142857, Blast_Score=1010, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=493, Percent_Identity=27.9918864097363, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI48994988, Length=152, Percent_Identity=39.4736842105263, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=37.3417721518987, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=693, Percent_Identity=40.8369408369408, Blast_Score=527, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17556745, Length=726, Percent_Identity=29.4765840220386, Blast_Score=315, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI17506493, Length=827, Percent_Identity=26.2394195888754, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17557151, Length=159, Percent_Identity=36.4779874213836, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=148, Percent_Identity=33.1081081081081, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=148, Percent_Identity=33.1081081081081, Blast_Score=76, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=704, Percent_Identity=43.0397727272727, Blast_Score=560, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6322359, Length=803, Percent_Identity=31.2577833125778, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324707, Length=482, Percent_Identity=26.7634854771784, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320593, Length=482, Percent_Identity=26.7634854771784, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=155, Percent_Identity=34.8387096774194, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=174, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582462, Length=708, Percent_Identity=43.0790960451977, Blast_Score=585, Evalue=1e-167,
Organism=Drosophila melanogaster, GI221458488, Length=734, Percent_Identity=32.8337874659401, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24585709, Length=816, Percent_Identity=26.593137254902, Blast_Score=192, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24585711, Length=817, Percent_Identity=26.8053855569155, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24585713, Length=817, Percent_Identity=26.8053855569155, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI78706572, Length=204, Percent_Identity=30.3921568627451, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574573, Length=208, Percent_Identity=32.6923076923077, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 79156; Mature: 79025

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTPLKRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVHDGAATMDWMEQEQERG
CCCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTCFWSGMAKQFDEHRINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ
EEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHCCCC
PQSETVWRQANKYKVPRLAFINKMDRVGADFYRVVEQIKTRLGGKPVPLVIPIGKEDDFE
CCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
GVVDLVTMKAIYWDEASQGMEYDEREIPAELQEKAEEYREYLVESAAEATEELMNEYLEN
HHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELTVDQINSAIRQLTIDNEIIPLLCGTAFKNKGVQKMLDAVIQYLPAPMDVPAIKGILD
CCEEHHHHHHHHHHHHCCCCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHCC
DKDESEGTREASDEAPFSALAFKIMNDKFVGNLTFVRVYSGVLKQGSSVYNPVKMKRERV
CCCCCCCCCCCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
GRIVQMMANSQEELQEIRTGDIAALVGMKDVTTGDTLCDEQNVITLERMEFPDPVISLAV
HHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHEEEE
EPKTKADQEKMSIALGRLAKEDPSFRVHTDEESGQTIISGMGELHLEILVDRMKREFGVE
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ANIGAPQVAYRETIRNTVEQEGKFVRQTGGRGKFGHVWLRLEPMDPAGDVLYEFKEEVVG
CCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHC
GTVPKEFHGAVDKGIQERMKNGVLAGYPIVGVKATLYDGSYHDVDSDELSFKMAGSIAFR
CCCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCCCCCEECCHHHHH
KGFMAANPTLLEPVMKVEVETPEDYMGDIMGDLNRRRGMVQGMEDLPGGTKQIRAEVPLA
CCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHCCCHH
EMFGYATQMRSMSQGRATYSMEFQKYAEIPKSVAEAIISKFNNKDDDE
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARKTPLKRYRNIGISAHIDAGKTTTTERVLFYTGVSHKIGEVHDGAATMDWMEQEQERG
CCCCHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
ITITSAATTCFWSGMAKQFDEHRINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ
EEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHCCCC
PQSETVWRQANKYKVPRLAFINKMDRVGADFYRVVEQIKTRLGGKPVPLVIPIGKEDDFE
CCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
GVVDLVTMKAIYWDEASQGMEYDEREIPAELQEKAEEYREYLVESAAEATEELMNEYLEN
HHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GELTVDQINSAIRQLTIDNEIIPLLCGTAFKNKGVQKMLDAVIQYLPAPMDVPAIKGILD
CCEEHHHHHHHHHHHHCCCCCHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHCC
DKDESEGTREASDEAPFSALAFKIMNDKFVGNLTFVRVYSGVLKQGSSVYNPVKMKRERV
CCCCCCCCCCCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
GRIVQMMANSQEELQEIRTGDIAALVGMKDVTTGDTLCDEQNVITLERMEFPDPVISLAV
HHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHEEEE
EPKTKADQEKMSIALGRLAKEDPSFRVHTDEESGQTIISGMGELHLEILVDRMKREFGVE
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ANIGAPQVAYRETIRNTVEQEGKFVRQTGGRGKFGHVWLRLEPMDPAGDVLYEFKEEVVG
CCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHC
GTVPKEFHGAVDKGIQERMKNGVLAGYPIVGVKATLYDGSYHDVDSDELSFKMAGSIAFR
CCCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCCCCCEECCHHHHH
KGFMAANPTLLEPVMKVEVETPEDYMGDIMGDLNRRRGMVQGMEDLPGGTKQIRAEVPLA
CCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHCCCHH
EMFGYATQMRSMSQGRATYSMEFQKYAEIPKSVAEAIISKFNNKDDDE
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA