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The map label for this gene is glyS [H]

Identifier: 93006879

GI number: 93006879

Start: 2522795

End: 2524888

Strand: Reverse

Name: glyS [H]

Synonym: Pcryo_2055

Alternate gene names: 93006879

Gene position: 2524888-2522795 (Counterclockwise)

Preceding gene: 93006880

Following gene: 93006878

Centisome position: 82.52

GC content: 44.75

Gene sequence:

>2094_bases
ATGAGTACTATTCTATTTGAACTGGGGTGTGAAGAGCTGCCTCCAAAAAGTCTAAAACCTTTACGTGACGCATTACAAGA
AAGTGTTACCGAGCAATTAGCAGACGCTAATATCAGCTTCGATAGTATCAAAGCCTTTGCGGCACCGCGTCGTTTAGCAA
TACAGATTCAAGGCATTAGCGATAAACAGCCGGATCGTACTGAGGAAAAACGTGGACCTGCGATTAAAGCTGCCTTTGAT
GCTGATGGTAATCCTACCCGTGCGGCGATGGGCTTTGCAAAAGGTTTGGGTATTGAAGCCAGCAAGTTGACCACTATTAA
TACCGATAAAGGTGATTACGTCGGTTATGTGCAAACGATTCAGGGTCAAGCAACCACTGAGCTGCTACCTGCTATTTTTC
AAACTGCACTTGATAATTTGCCGATTGCTAAACGTATGCGTTCAGGCGCGAGCCGTAATGAGTTCGTGCGTCCCATACAG
TGGGCAGTCTTGATGCAAAATGATGCTGTCGTTGATGCCACTATCCAAGGTCATCAGACAGGGACGCAAACTCGTGGTCA
TCGCTTCCATAGCCCTGATTACCATAACATTGCGCATGCCAATGATTATGAGCAACTGCTTGATGGTTTAAAAGTCGTAG
CTGATTTTGATAAGCGTCAAATGCTGATTAAAAACCAAGTCAAGGCATTGGCAGATGAGGTTAGTTCTGACGCTATCGTG
CCGCAGAGTTTATTAGATGAAGTCACCGCATTGGTTGATTTCCCTATTGCCCTGCGTGCAAGCTTTGAAGCGCGTTTTTT
ACAAGTGCCGCAAGAAGCGCTTATCTCTACCATGCAAGCGGATCAAAAGTATTTTTGCTTAACCGATAAAGCAGGCAAAT
TGCAGCCTTACTTTATTTTTATTACCAATATTGAGTCAAAAGATCCTAATCAAATTATTGAAGGTAATGAGAAAGTCGTG
CGTCCACGCTTGGCTGATGCAGAGTTTTTCTTCTTACAAGATCAAAAGCAGCCATTATTTGCGCTGACAGAAAGCCTAAA
AACTCGCGTCTTCCAAGACAAGCTGGGTACGATTTGGGAAAAATCTGAGCGCATTGCTAAGCTTGCCGCCTTTATTGCCG
CCTTAATGCAACAGCAAGGTCAGCAAATTGATATCGATCAAACAGTGCGTGCAGGTATTTTGTCCAAAGCAGACTTGGCA
AGTTCACTGGTCGGTGAATATCCTGAATTACAAGGTATCGCTGGTACTTACTATGCACGTTTGAATGATGAGCCAGAAGC
CGTAGCAGCCAGTTTAGAAGAGCAGTATTTGCCCAAGTTCAGTGGTGATGTATTACCACAGACGCCAGTTGGTATTTGCT
TAGCATTAGCAGATCGTTTGGACACGCTCGTCGGTATTTTTGCCATTGACCAAGCACCGACAGGTTCAAAAGATCCGTTC
AGTTTACGTCGTTCAGCGATTGGTATCCTGCGTATTCTCATTGAAAAGCAGCTGCCAATTAATCTAGTGGCTCTCATTGA
GCAAGCCATTAAGGGCTATAGTACCGCGGAAGGTAGCAAGATTGACAAGATGGGCGACACATTTACCCAAGTGATGGCAT
TTTTGAACTCGCGCTATCGGGCGATGTATACCGAGCAAGGCGTTAACGTCGATACCATCCAAGCAGTACAGGCGATTAAC
CCGCATATGCCGCTTGATTTTGATCAGCGTATTCGTGCCGTTCAAACGTTTAGCAACTTACCTCAAGCGTCGATGCTTGC
TGATTCAAACAAACGTGTCGCCAATATTTTAGCCAAGTCAGAAGTGAGCGTTGCTGACACTGTTGATGAAGCTCTACTAT
CTGAACCTGCTGAGCAAAATTTATATGCGAGTGTGAAGCAAGCACAGACAGTGGTACAACCATTACTTGAGCAGGCTGAC
TATACGCAAGTATTGCAAACGCTTGCTAGCTTAGATGAGCCGTTGACGCAATTCTTTGATAATGTGATGGTCAACAGTGA
AGATACGGCGCTTAAAGCCAACCGCTTAGCGTTGCTTAAGCAAGTACGCGCATTGTTTTTAACTGTAGCTGATATCAGTG
AGCTACAGTTATAG

Upstream 100 bases:

>100_bases
TCGTCAAGCGGCGCTCGATAAGTATTTGCCAAAAGAAGATGTGGCAACTGAAAACACAGATAACAACCAAACTACTAATA
ACAATAAATAGGAGCATCCC

Downstream 100 bases:

>100_bases
TTTGGTCCCTGATAGCTTAGTCACTATAACGGCTAAATAATGTATTCTATAAAATAAAAACTGGCTCAATGTGGCCAGTT
TTTTATATGATTATTCTGAA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSTILFELGCEELPPKSLKPLRDALQESVTEQLADANISFDSIKAFAAPRRLAIQIQGISDKQPDRTEEKRGPAIKAAFD
ADGNPTRAAMGFAKGLGIEASKLTTINTDKGDYVGYVQTIQGQATTELLPAIFQTALDNLPIAKRMRSGASRNEFVRPIQ
WAVLMQNDAVVDATIQGHQTGTQTRGHRFHSPDYHNIAHANDYEQLLDGLKVVADFDKRQMLIKNQVKALADEVSSDAIV
PQSLLDEVTALVDFPIALRASFEARFLQVPQEALISTMQADQKYFCLTDKAGKLQPYFIFITNIESKDPNQIIEGNEKVV
RPRLADAEFFFLQDQKQPLFALTESLKTRVFQDKLGTIWEKSERIAKLAAFIAALMQQQGQQIDIDQTVRAGILSKADLA
SSLVGEYPELQGIAGTYYARLNDEPEAVAASLEEQYLPKFSGDVLPQTPVGICLALADRLDTLVGIFAIDQAPTGSKDPF
SLRRSAIGILRILIEKQLPINLVALIEQAIKGYSTAEGSKIDKMGDTFTQVMAFLNSRYRAMYTEQGVNVDTIQAVQAIN
PHMPLDFDQRIRAVQTFSNLPQASMLADSNKRVANILAKSEVSVADTVDEALLSEPAEQNLYASVKQAQTVVQPLLEQAD
YTQVLQTLASLDEPLTQFFDNVMVNSEDTALKANRLALLKQVRALFLTVADISELQL

Sequences:

>Translated_697_residues
MSTILFELGCEELPPKSLKPLRDALQESVTEQLADANISFDSIKAFAAPRRLAIQIQGISDKQPDRTEEKRGPAIKAAFD
ADGNPTRAAMGFAKGLGIEASKLTTINTDKGDYVGYVQTIQGQATTELLPAIFQTALDNLPIAKRMRSGASRNEFVRPIQ
WAVLMQNDAVVDATIQGHQTGTQTRGHRFHSPDYHNIAHANDYEQLLDGLKVVADFDKRQMLIKNQVKALADEVSSDAIV
PQSLLDEVTALVDFPIALRASFEARFLQVPQEALISTMQADQKYFCLTDKAGKLQPYFIFITNIESKDPNQIIEGNEKVV
RPRLADAEFFFLQDQKQPLFALTESLKTRVFQDKLGTIWEKSERIAKLAAFIAALMQQQGQQIDIDQTVRAGILSKADLA
SSLVGEYPELQGIAGTYYARLNDEPEAVAASLEEQYLPKFSGDVLPQTPVGICLALADRLDTLVGIFAIDQAPTGSKDPF
SLRRSAIGILRILIEKQLPINLVALIEQAIKGYSTAEGSKIDKMGDTFTQVMAFLNSRYRAMYTEQGVNVDTIQAVQAIN
PHMPLDFDQRIRAVQTFSNLPQASMLADSNKRVANILAKSEVSVADTVDEALLSEPAEQNLYASVKQAQTVVQPLLEQAD
YTQVLQTLASLDEPLTQFFDNVMVNSEDTALKANRLALLKQVRALFLTVADISELQL
>Mature_696_residues
STILFELGCEELPPKSLKPLRDALQESVTEQLADANISFDSIKAFAAPRRLAIQIQGISDKQPDRTEEKRGPAIKAAFDA
DGNPTRAAMGFAKGLGIEASKLTTINTDKGDYVGYVQTIQGQATTELLPAIFQTALDNLPIAKRMRSGASRNEFVRPIQW
AVLMQNDAVVDATIQGHQTGTQTRGHRFHSPDYHNIAHANDYEQLLDGLKVVADFDKRQMLIKNQVKALADEVSSDAIVP
QSLLDEVTALVDFPIALRASFEARFLQVPQEALISTMQADQKYFCLTDKAGKLQPYFIFITNIESKDPNQIIEGNEKVVR
PRLADAEFFFLQDQKQPLFALTESLKTRVFQDKLGTIWEKSERIAKLAAFIAALMQQQGQQIDIDQTVRAGILSKADLAS
SLVGEYPELQGIAGTYYARLNDEPEAVAASLEEQYLPKFSGDVLPQTPVGICLALADRLDTLVGIFAIDQAPTGSKDPFS
LRRSAIGILRILIEKQLPINLVALIEQAIKGYSTAEGSKIDKMGDTFTQVMAFLNSRYRAMYTEQGVNVDTIQAVQAINP
HMPLDFDQRIRAVQTFSNLPQASMLADSNKRVANILAKSEVSVADTVDEALLSEPAEQNLYASVKQAQTVVQPLLEQADY
TQVLQTLASLDEPLTQFFDNVMVNSEDTALKANRLALLKQVRALFLTVADISELQL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=695, Percent_Identity=46.4748201438849, Blast_Score=605, Evalue=1e-174,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76911; Mature: 76779

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILFELGCEELPPKSLKPLRDALQESVTEQLADANISFDSIKAFAAPRRLAIQIQGIS
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCC
DKQPDRTEEKRGPAIKAAFDADGNPTRAAMGFAKGLGIEASKLTTINTDKGDYVGYVQTI
CCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEHHHHH
QGQATTELLPAIFQTALDNLPIAKRMRSGASRNEFVRPIQWAVLMQNDAVVDATIQGHQT
CCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEEEECCCCEEEEEECCCCC
GTQTRGHRFHSPDYHNIAHANDYEQLLDGLKVVADFDKRQMLIKNQVKALADEVSSDAIV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQSLLDEVTALVDFPIALRASFEARFLQVPQEALISTMQADQKYFCLTDKAGKLQPYFIF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
ITNIESKDPNQIIEGNEKVVRPRLADAEFFFLQDQKQPLFALTESLKTRVFQDKLGTIWE
EEECCCCCCHHHHCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KSERIAKLAAFIAALMQQQGQQIDIDQTVRAGILSKADLASSLVGEYPELQGIAGTYYAR
HHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEE
LNDEPEAVAASLEEQYLPKFSGDVLPQTPVGICLALADRLDTLVGIFAIDQAPTGSKDPF
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHH
SLRRSAIGILRILIEKQLPINLVALIEQAIKGYSTAEGSKIDKMGDTFTQVMAFLNSRYR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AMYTEQGVNVDTIQAVQAINPHMPLDFDQRIRAVQTFSNLPQASMLADSNKRVANILAKS
HHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHC
EVSVADTVDEALLSEPAEQNLYASVKQAQTVVQPLLEQADYTQVLQTLASLDEPLTQFFD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NVMVNSEDTALKANRLALLKQVRALFLTVADISELQL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STILFELGCEELPPKSLKPLRDALQESVTEQLADANISFDSIKAFAAPRRLAIQIQGIS
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCC
DKQPDRTEEKRGPAIKAAFDADGNPTRAAMGFAKGLGIEASKLTTINTDKGDYVGYVQTI
CCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEHHHHH
QGQATTELLPAIFQTALDNLPIAKRMRSGASRNEFVRPIQWAVLMQNDAVVDATIQGHQT
CCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEEEECCCCEEEEEECCCCC
GTQTRGHRFHSPDYHNIAHANDYEQLLDGLKVVADFDKRQMLIKNQVKALADEVSSDAIV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQSLLDEVTALVDFPIALRASFEARFLQVPQEALISTMQADQKYFCLTDKAGKLQPYFIF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
ITNIESKDPNQIIEGNEKVVRPRLADAEFFFLQDQKQPLFALTESLKTRVFQDKLGTIWE
EEECCCCCCHHHHCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
KSERIAKLAAFIAALMQQQGQQIDIDQTVRAGILSKADLASSLVGEYPELQGIAGTYYAR
HHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEE
LNDEPEAVAASLEEQYLPKFSGDVLPQTPVGICLALADRLDTLVGIFAIDQAPTGSKDPF
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHH
SLRRSAIGILRILIEKQLPINLVALIEQAIKGYSTAEGSKIDKMGDTFTQVMAFLNSRYR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AMYTEQGVNVDTIQAVQAINPHMPLDFDQRIRAVQTFSNLPQASMLADSNKRVANILAKS
HHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHC
EVSVADTVDEALLSEPAEQNLYASVKQAQTVVQPLLEQADYTQVLQTLASLDEPLTQFFD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NVMVNSEDTALKANRLALLKQVRALFLTVADISELQL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA