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The map label for this gene is mdh [H]

Identifier: 93006868

GI number: 93006868

Start: 2510939

End: 2511928

Strand: Reverse

Name: mdh [H]

Synonym: Pcryo_2044

Alternate gene names: 93006868

Gene position: 2511928-2510939 (Counterclockwise)

Preceding gene: 93006869

Following gene: 93006867

Centisome position: 82.09

GC content: 47.27

Gene sequence:

>990_bases
ATGTCAATGAAACAGCCTGTACGCGTTGCCGTGACTGGTGCCGCTGGTAATATCAGCTATGCTATGTTATTTCGTATTGC
TTCTGGCGAGATGCTAGGCAAAGATCAGCCAGTGATTCTGCAATTACTTGAAATCGCTCCAGCACTCGACGCCCTAAAGG
GTGTGGTCATGGAATTAGAAGACTGTGCATTCCCATTATTGGCTGGTGTCGTCCAAACCGATGATGCGACCGTGGCTTTT
AAAGATGTCGATTATGCGTTACTCGTAGGCTCACGCCCTCGTGGTCCTGGTATGGAACGTAAGGATTTGCTAGAAGCCAA
TGCTGCGATTTTCTCAGCGCAAGGTAAAGCATTGAATGATGTTGCAAGCCGTGATGTTAAAGTATTAGTAGTAGGTAATC
CTGCTAACACCAACGCTGTTATCGCTCAGCGTAACGCACCGGATTTAGATCCACGTAACTTCACTGCCATGACTCGCCTA
GATCATAACCGTGCAATGGCACAGTTAGCTGGCAAGACTGACAGCACGGTTAATGACGTGAAGAAAATGATCATCTGGGG
CAACCATTCATCAACTCAGTATCCTGATTTGACGGCGAGCACTGTAAATGGCAAACCAGCGTTAGACTTGGTTGATCGCG
CATGGTATGAAGGTACTTATATCCCAGAAGTACAACAACGTGGCGCTGCAATCATCAAAGCACGCGGTGCATCATCTGCT
GCTTCTGCTGCCAACGCGGCTATCGCACATGTACGCACGTGGGTAATGGGTACTGATGAGAATGATTGGGTATCTATGGG
TGTCTATTCAAACGGCGAGTACGGTATTGCTAAAGGTTTGATTTACTCATTCCCAGTAACTTGTGCTAACGGCGATTGGT
CTATCGTAGATGGTGTTGATGTGTCATCAGATTTCTCAAAAGAGAAAATGGCAGCCACTGAGCAAGAGCTGAGCGAAGAG
CGTGATGCAGTGGCACACTTATTACCGTAA

Upstream 100 bases:

>100_bases
CTATAGTAATCTAGTAAGCATTCAGCGAGTAAGTCGCTATCTAAATCGATAATCACCAATTAGAATCGTGAATATCTAAT
CAATAATTAGGGAGTATTCA

Downstream 100 bases:

>100_bases
GCTTTTAGGCTATTGTCATATTAGGGCTGTTTATATTTAGCACTAAGTAAAAAGCCCTCATTTATCATGAGGGCTTTTTT
TGTTTGAATTTTAGCGTGCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSMKQPVRVAVTGAAGNISYAMLFRIASGEMLGKDQPVILQLLEIAPALDALKGVVMELEDCAFPLLAGVVQTDDATVAF
KDVDYALLVGSRPRGPGMERKDLLEANAAIFSAQGKALNDVASRDVKVLVVGNPANTNAVIAQRNAPDLDPRNFTAMTRL
DHNRAMAQLAGKTDSTVNDVKKMIIWGNHSSTQYPDLTASTVNGKPALDLVDRAWYEGTYIPEVQQRGAAIIKARGASSA
ASAANAAIAHVRTWVMGTDENDWVSMGVYSNGEYGIAKGLIYSFPVTCANGDWSIVDGVDVSSDFSKEKMAATEQELSEE
RDAVAHLLP

Sequences:

>Translated_329_residues
MSMKQPVRVAVTGAAGNISYAMLFRIASGEMLGKDQPVILQLLEIAPALDALKGVVMELEDCAFPLLAGVVQTDDATVAF
KDVDYALLVGSRPRGPGMERKDLLEANAAIFSAQGKALNDVASRDVKVLVVGNPANTNAVIAQRNAPDLDPRNFTAMTRL
DHNRAMAQLAGKTDSTVNDVKKMIIWGNHSSTQYPDLTASTVNGKPALDLVDRAWYEGTYIPEVQQRGAAIIKARGASSA
ASAANAAIAHVRTWVMGTDENDWVSMGVYSNGEYGIAKGLIYSFPVTCANGDWSIVDGVDVSSDFSKEKMAATEQELSEE
RDAVAHLLP
>Mature_328_residues
SMKQPVRVAVTGAAGNISYAMLFRIASGEMLGKDQPVILQLLEIAPALDALKGVVMELEDCAFPLLAGVVQTDDATVAFK
DVDYALLVGSRPRGPGMERKDLLEANAAIFSAQGKALNDVASRDVKVLVVGNPANTNAVIAQRNAPDLDPRNFTAMTRLD
HNRAMAQLAGKTDSTVNDVKKMIIWGNHSSTQYPDLTASTVNGKPALDLVDRAWYEGTYIPEVQQRGAAIIKARGASSAA
SAANAAIAHVRTWVMGTDENDWVSMGVYSNGEYGIAKGLIYSFPVTCANGDWSIVDGVDVSSDFSKEKMAATEQELSEER
DAVAHLLP

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=332, Percent_Identity=54.2168674698795, Blast_Score=356, Evalue=1e-98,
Organism=Homo sapiens, GI89886456, Length=332, Percent_Identity=26.2048192771084, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=53.9877300613497, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=53.8167938931298, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI32566800, Length=171, Percent_Identity=53.2163742690059, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24583394, Length=331, Percent_Identity=53.4743202416918, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35157; Mature: 35026

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKQPVRVAVTGAAGNISYAMLFRIASGEMLGKDQPVILQLLEIAPALDALKGVVMELE
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DCAFPLLAGVVQTDDATVAFKDVDYALLVGSRPRGPGMERKDLLEANAAIFSAQGKALND
HHHHHHHHHHEECCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCHHHH
VASRDVKVLVVGNPANTNAVIAQRNAPDLDPRNFTAMTRLDHNRAMAQLAGKTDSTVNDV
HHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHH
KKMIIWGNHSSTQYPDLTASTVNGKPALDLVDRAWYEGTYIPEVQQRGAAIIKARGASSA
EEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCH
ASAANAAIAHVRTWVMGTDENDWVSMGVYSNGEYGIAKGLIYSFPVTCANGDWSIVDGVD
HHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCCCHHHCEEEECCEEECCCCEEEEECCC
VSSDFSKEKMAATEQELSEERDAVAHLLP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SMKQPVRVAVTGAAGNISYAMLFRIASGEMLGKDQPVILQLLEIAPALDALKGVVMELE
CCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DCAFPLLAGVVQTDDATVAFKDVDYALLVGSRPRGPGMERKDLLEANAAIFSAQGKALND
HHHHHHHHHHEECCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCHHHH
VASRDVKVLVVGNPANTNAVIAQRNAPDLDPRNFTAMTRLDHNRAMAQLAGKTDSTVNDV
HHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHH
KKMIIWGNHSSTQYPDLTASTVNGKPALDLVDRAWYEGTYIPEVQQRGAAIIKARGASSA
EEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCH
ASAANAAIAHVRTWVMGTDENDWVSMGVYSNGEYGIAKGLIYSFPVTCANGDWSIVDGVD
HHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCCCHHHCEEEECCEEECCCCEEEEECCC
VSSDFSKEKMAATEQELSEERDAVAHLLP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA