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The map label for this gene is ispD

Identifier: 93006692

GI number: 93006692

Start: 2307618

End: 2308460

Strand: Reverse

Name: ispD

Synonym: Pcryo_1868

Alternate gene names: 93006692

Gene position: 2308460-2307618 (Counterclockwise)

Preceding gene: 93006693

Following gene: 93006691

Centisome position: 75.44

GC content: 44.6

Gene sequence:

>843_bases
ATGGAATCAACCCATGAGTACTGCATTATGAATACCACTCCTAATATCCATGCCATGATCGTTGCTGCTGGACGCGGTAG
TCGCTTTGGCGCGTCTATTGCTAAACAATATACCTTACTGCAAGGTCAAACCTTATTACAGCATAGTGTGGCACGTCTGG
CTGAGAGTAATTATATTGATCGATGTCTATTGGTTGTGGCTCAAGATGATAGCACTGCCCAGACACTGAGCTTTGCGCTT
CCTATCTATTATGCTATTGGCGGTACTGAACGTTGGCAATCGGTGCAGGCAGGAGTCGAGGCAATGATTAATGCGGGCGC
AGATGAGTCGGATTTGGTTGTGATCCATGATGCAGCTCGTCCTGCAGTGCCAACTCATGATATTGATGCCGTCATTCAAG
CAGCGATGCTAGAGCCTTATGGTGCTATTTTAGCGACACCGGTTGCTGATACCCTAAAACAGTCTTACATAGCATCTAAC
ACACTATCAACGCCAGCATATGAAGCTCCACTATCTCTTCAGCAACTGAATACTCAGCCGGAGCTTGCTAATAGTCAAAG
CGGTTATGACACGCTTCATACTTATGCTCAAAAAACTATTGATCGCAGCCATATGTGGCAGGCACAAACACCGCAAGTAT
TTAGGCTTGGACAATTGCAACAAGTCTTGAACTATGTCACTAAGCATAATCTGGCCATTACTGACGAAGCCAGTGCTTTT
GAGCATTTAGAACTGCCAATTCGATTGGTAACAGGCAGTCGTCAAAACATCAAACTGACTTATCCTGATGATATTATTTT
ACTGACAGCCATTCTCAAGGCTCAATTCGCCTCTATCTCATAA

Upstream 100 bases:

>100_bases
GTATTGATTCTAAAGTCGTTGCAATGAGCTATTATTGATAATAATAGTTTTTTTTCATTTTTATACCTCTTAATTCCCAT
TTTTCTCTTATCCTATTTTC

Downstream 100 bases:

>100_bases
AATTCTTATCACACTCTGATTTCTGTATTTTTTAACTTTATATATTTTATATAACTTTTTTATTCTGTCATAAAAATGTC
GAATATTCATTGTATCTATC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MESTHEYCIMNTTPNIHAMIVAAGRGSRFGASIAKQYTLLQGQTLLQHSVARLAESNYIDRCLLVVAQDDSTAQTLSFAL
PIYYAIGGTERWQSVQAGVEAMINAGADESDLVVIHDAARPAVPTHDIDAVIQAAMLEPYGAILATPVADTLKQSYIASN
TLSTPAYEAPLSLQQLNTQPELANSQSGYDTLHTYAQKTIDRSHMWQAQTPQVFRLGQLQQVLNYVTKHNLAITDEASAF
EHLELPIRLVTGSRQNIKLTYPDDIILLTAILKAQFASIS

Sequences:

>Translated_280_residues
MESTHEYCIMNTTPNIHAMIVAAGRGSRFGASIAKQYTLLQGQTLLQHSVARLAESNYIDRCLLVVAQDDSTAQTLSFAL
PIYYAIGGTERWQSVQAGVEAMINAGADESDLVVIHDAARPAVPTHDIDAVIQAAMLEPYGAILATPVADTLKQSYIASN
TLSTPAYEAPLSLQQLNTQPELANSQSGYDTLHTYAQKTIDRSHMWQAQTPQVFRLGQLQQVLNYVTKHNLAITDEASAF
EHLELPIRLVTGSRQNIKLTYPDDIILLTAILKAQFASIS
>Mature_280_residues
MESTHEYCIMNTTPNIHAMIVAAGRGSRFGASIAKQYTLLQGQTLLQHSVARLAESNYIDRCLLVVAQDDSTAQTLSFAL
PIYYAIGGTERWQSVQAGVEAMINAGADESDLVVIHDAARPAVPTHDIDAVIQAAMLEPYGAILATPVADTLKQSYIASN
TLSTPAYEAPLSLQQLNTQPELANSQSGYDTLHTYAQKTIDRSHMWQAQTPQVFRLGQLQQVLNYVTKHNLAITDEASAF
EHLELPIRLVTGSRQNIKLTYPDDIILLTAILKAQFASIS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=255, Percent_Identity=30.9803921568627, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PSYCK (Q1Q9K8)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_581129.1
- ProteinModelPortal:   Q1Q9K8
- STRING:   Q1Q9K8
- GeneID:   4034389
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1868
- NMPDR:   fig|335284.3.peg.2277
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   Q1Q9K8
- ProtClustDB:   CLSK839390
- BioCyc:   PCRY335284:PCRYO_1868-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 30626; Mature: 30626

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTHEYCIMNTTPNIHAMIVAAGRGSRFGASIAKQYTLLQGQTLLQHSVARLAESNYID
CCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
RCLLVVAQDDSTAQTLSFALPIYYAIGGTERWQSVQAGVEAMINAGADESDLVVIHDAAR
EEEEEEECCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PAVPTHDIDAVIQAAMLEPYGAILATPVADTLKQSYIASNTLSTPAYEAPLSLQQLNTQP
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
ELANSQSGYDTLHTYAQKTIDRSHMWQAQTPQVFRLGQLQQVLNYVTKHNLAITDEASAF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHH
EHLELPIRLVTGSRQNIKLTYPDDIILLTAILKAQFASIS
HHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MESTHEYCIMNTTPNIHAMIVAAGRGSRFGASIAKQYTLLQGQTLLQHSVARLAESNYID
CCCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
RCLLVVAQDDSTAQTLSFALPIYYAIGGTERWQSVQAGVEAMINAGADESDLVVIHDAAR
EEEEEEECCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
PAVPTHDIDAVIQAAMLEPYGAILATPVADTLKQSYIASNTLSTPAYEAPLSLQQLNTQP
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
ELANSQSGYDTLHTYAQKTIDRSHMWQAQTPQVFRLGQLQQVLNYVTKHNLAITDEASAF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHH
EHLELPIRLVTGSRQNIKLTYPDDIILLTAILKAQFASIS
HHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA