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The map label for this gene is argG [H]

Identifier: 93006688

GI number: 93006688

Start: 2302170

End: 2303408

Strand: Reverse

Name: argG [H]

Synonym: Pcryo_1864

Alternate gene names: 93006688

Gene position: 2303408-2302170 (Counterclockwise)

Preceding gene: 93006691

Following gene: 93006686

Centisome position: 75.28

GC content: 45.04

Gene sequence:

>1239_bases
ATGGCTCAGCTTGATCCAAAAACTATTAATAAAATCGTATTGGCATATTCAGGGGGTCTTGACACCTCAATCATCGCTCG
ATGGCTACAAGAAACCTATGATGCAGAAGTGATCACTTTCACTGCGGATATTGGTCAAGGTGAAGAAGTTGAGCCTGCGC
GTGCCAAAGCTGAAGCCATGGGTATCAAACATATCCATATTGAAGACTTGCGCGAAGAATTTGCTCGTGATTACGTGTTC
CCAATGTTCCGTGCCAATGCCATCTACGAAGGTGAGTATCTGCTTGGTACTTCTATCGCGCGTCCACTCATCGCTAAGCG
TTTGGTTGAAATCGCTAAAGAACATAATGCTGATGCGATCAGCCATGGTGCAACCGGTAAAGGTAATGACCAAGTACGCT
TTGAGCTTGGTGCCGTCGCTTTATCTCCAGACGTCGTGACTATCGCGCCTTGGCGTGAGTGGGATTTATCGAGCCGTGAA
AGCTTGATGGAATATGCTAAAGAGCATAATATTGCCATCGACTATGCGGGCAATAAGAAAAAATCTCCGTACTCGATGGA
TGCCAATTTATTACATATCTCTTATGAAGGCGGCATTTTAGAAGATCCGTATGCTGAAGCAGAAGATGACATGTGGCGCT
GGTCAGTAAGCCCAGAAGAAGCACCAGATGAAGCCCAATATCTGGAATTAGAGTATGAAAAAGGCGACATCGTTGCCATC
GATGGTGAAGCGCTTAAGCCATATGAAGTAATGATTAAGCTTAATGAGATTGGTGGTAAGCATGGTATCGGACGTTTGGA
TATCGTTGAAAACCGTTACGTCGGTATGAAGTCGCGCGGCTGCTACGAGACGCCAGCGGGCACCATTATGTTAAAAGCGC
ATCGTGGTATTGAGTCATTGACACTTGACCGTGAAGCGGCGCATCTAAAAGATGAGCTGATGCCACGTTATGCTAAGACT
ATCTACAACGGCTATTGGTTCAGCCCTGAGCGTATGATGCTACAGGCGCTGATTGATAAGTCACAAGAGTATGTTAACGG
TACGGTACGTGTCAAATTATACAAAGGTGCTGTAAGCGTGGTTGGTCGTAAGTCAGATGATTCGTTATTTGATGAAAAAA
TTGCGACTTTTGAAGATGATGCGGGCGCCTATGATCAAAAAGATGCAGAAGGTTTCATTCGTCTAAATGGTTTACGTTTG
GCAATTGAAGCCAGCCGTGGTCGCGATTTATCAAAATAA

Upstream 100 bases:

>100_bases
TTGCCAATGGTAAATATGATGTACACTGAGCGAGCAATCTATCTTCCTCGTGATATAGTCGATTTACCTATCATTAAATT
CCTCATACTAGGAGTGTTTC

Downstream 100 bases:

>100_bases
GCAACGTTAACGAAAGGTTTTTTAAGTCCCTGATGAATTAACTGACCGATAAAAAAGAGTCGCTATTTATATAGCGCCTC
TTTTTTTATTGCTGATAGGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAQLDPKTINKIVLAYSGGLDTSIIARWLQETYDAEVITFTADIGQGEEVEPARAKAEAMGIKHIHIEDLREEFARDYVF
PMFRANAIYEGEYLLGTSIARPLIAKRLVEIAKEHNADAISHGATGKGNDQVRFELGAVALSPDVVTIAPWREWDLSSRE
SLMEYAKEHNIAIDYAGNKKKSPYSMDANLLHISYEGGILEDPYAEAEDDMWRWSVSPEEAPDEAQYLELEYEKGDIVAI
DGEALKPYEVMIKLNEIGGKHGIGRLDIVENRYVGMKSRGCYETPAGTIMLKAHRGIESLTLDREAAHLKDELMPRYAKT
IYNGYWFSPERMMLQALIDKSQEYVNGTVRVKLYKGAVSVVGRKSDDSLFDEKIATFEDDAGAYDQKDAEGFIRLNGLRL
AIEASRGRDLSK

Sequences:

>Translated_412_residues
MAQLDPKTINKIVLAYSGGLDTSIIARWLQETYDAEVITFTADIGQGEEVEPARAKAEAMGIKHIHIEDLREEFARDYVF
PMFRANAIYEGEYLLGTSIARPLIAKRLVEIAKEHNADAISHGATGKGNDQVRFELGAVALSPDVVTIAPWREWDLSSRE
SLMEYAKEHNIAIDYAGNKKKSPYSMDANLLHISYEGGILEDPYAEAEDDMWRWSVSPEEAPDEAQYLELEYEKGDIVAI
DGEALKPYEVMIKLNEIGGKHGIGRLDIVENRYVGMKSRGCYETPAGTIMLKAHRGIESLTLDREAAHLKDELMPRYAKT
IYNGYWFSPERMMLQALIDKSQEYVNGTVRVKLYKGAVSVVGRKSDDSLFDEKIATFEDDAGAYDQKDAEGFIRLNGLRL
AIEASRGRDLSK
>Mature_411_residues
AQLDPKTINKIVLAYSGGLDTSIIARWLQETYDAEVITFTADIGQGEEVEPARAKAEAMGIKHIHIEDLREEFARDYVFP
MFRANAIYEGEYLLGTSIARPLIAKRLVEIAKEHNADAISHGATGKGNDQVRFELGAVALSPDVVTIAPWREWDLSSRES
LMEYAKEHNIAIDYAGNKKKSPYSMDANLLHISYEGGILEDPYAEAEDDMWRWSVSPEEAPDEAQYLELEYEKGDIVAID
GEALKPYEVMIKLNEIGGKHGIGRLDIVENRYVGMKSRGCYETPAGTIMLKAHRGIESLTLDREAAHLKDELMPRYAKTI
YNGYWFSPERMMLQALIDKSQEYVNGTVRVKLYKGAVSVVGRKSDDSLFDEKIATFEDDAGAYDQKDAEGFIRLNGLRLA
IEASRGRDLSK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=399, Percent_Identity=48.6215538847118, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI16950633, Length=399, Percent_Identity=48.6215538847118, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1789563, Length=411, Percent_Identity=26.0340632603406, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=42.0792079207921, Blast_Score=335, Evalue=8e-93,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=43.0348258706468, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 46245; Mature: 46114

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLDPKTINKIVLAYSGGLDTSIIARWLQETYDAEVITFTADIGQGEEVEPARAKAEAM
CCCCCHHHHCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHH
GIKHIHIEDLREEFARDYVFPMFRANAIYEGEYLLGTSIARPLIAKRLVEIAKEHNADAI
CEEEEEHHHHHHHHHHHHHCHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCCHH
SHGATGKGNDQVRFELGAVALSPDVVTIAPWREWDLSSRESLMEYAKEHNIAIDYAGNKK
CCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCC
KSPYSMDANLLHISYEGGILEDPYAEAEDDMWRWSVSPEEAPDEAQYLELEYEKGDIVAI
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEE
DGEALKPYEVMIKLNEIGGKHGIGRLDIVENRYVGMKSRGCYETPAGTIMLKAHRGIESL
CCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCE
TLDREAAHLKDELMPRYAKTIYNGYWFSPERMMLQALIDKSQEYVNGTVRVKLYKGAVSV
ECCHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHCCHHHCCCEEEEEEEECEEEE
VGRKSDDSLFDEKIATFEDDAGAYDQKDAEGFIRLNGLRLAIEASRGRDLSK
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
AQLDPKTINKIVLAYSGGLDTSIIARWLQETYDAEVITFTADIGQGEEVEPARAKAEAM
CCCCHHHHCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHH
GIKHIHIEDLREEFARDYVFPMFRANAIYEGEYLLGTSIARPLIAKRLVEIAKEHNADAI
CEEEEEHHHHHHHHHHHHHCHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHHHCCCCHH
SHGATGKGNDQVRFELGAVALSPDVVTIAPWREWDLSSRESLMEYAKEHNIAIDYAGNKK
CCCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCC
KSPYSMDANLLHISYEGGILEDPYAEAEDDMWRWSVSPEEAPDEAQYLELEYEKGDIVAI
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEE
DGEALKPYEVMIKLNEIGGKHGIGRLDIVENRYVGMKSRGCYETPAGTIMLKAHRGIESL
CCCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCE
TLDREAAHLKDELMPRYAKTIYNGYWFSPERMMLQALIDKSQEYVNGTVRVKLYKGAVSV
ECCHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHCCHHHCCCEEEEEEEECEEEE
VGRKSDDSLFDEKIATFEDDAGAYDQKDAEGFIRLNGLRLAIEASRGRDLSK
EECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA