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The map label for this gene is opuD [H]

Identifier: 93006637

GI number: 93006637

Start: 2237876

End: 2239561

Strand: Reverse

Name: opuD [H]

Synonym: Pcryo_1813

Alternate gene names: 93006637

Gene position: 2239561-2237876 (Counterclockwise)

Preceding gene: 93006638

Following gene: 93006636

Centisome position: 73.19

GC content: 45.97

Gene sequence:

>1686_bases
ATGGCTGATTTAAAAGGATTTAACAGTAAGCAACATGAGCAAGGACGCTCTCATAAAACCGATAAGATAGCGGATAATTT
AATGCATGTAGAGACTCCATCAGATACTTTAGGGCTAAAAAACCCAGCATTTTGGTACAGTGGCGGCTTTATCATTGCCT
TTGTTCTTATGGCCATCTTTGCCGAAGAGCGTTTGGGAAAGCTCATCAATACCGGATTTTCTTGGTCGGCAACGGTCTTT
GGTCCTTTTTGGCAAATTTTATTGTTAGCGACCTTTGTGATTGCACTAGCAGTCGGCGCAGGTCGTACAGGACGCGTCAT
TTTAGGTAATCTAGCGAAGCCTGAGATGGACGGTTTTAAATGGATGGCGATTTTATTCTGTACGCTGCTCGCTGGCGGCG
GCGTCTTTTGGGCAGCCGCTGAACCTATCGCTCATTTCGTTAGCGCACCGCCACTGTATGGCGAATCTGCTGATATCCAA
CAACGCGCTTTCAATGCGCTATCTCAGTCCTTTATGCATTGGGGATTTCTAGCTTGGTCAATTGTCGGTAGCTTGACGGC
TATTGTGGTGATGCATCTACATTATGATAAAGGCTTGCCGCTGAAACCGCGTACGCTTTTATACCCTATGTTTGGTGAGC
GCGTGCTTACAGGTCATACGGGTGCAATTATCGACGCTTGCTGTATTGTCGCGGTTGCTGCCGGTACGATCGGTCCTATT
GGATTTTTGGGATTACAAACCAGCTATGCGTTGAATACGCTGTTTGGTATTCCTGATACCTTTACTACCCAACTTATCAT
TATCATCTTTGCTATTGCACTTTATACGCTCTCAGCCATTAGTGGTCTTGCTCGAGGTATGCAGCTGCTCAGTCGCTTTA
ATGTCATATTGGCCTTTGGTCTGATGGTCTTTATTTTGATGTTTGGTCCTACCAAATTTATTGTTAATGGGTATATTCAA
GGTGTTGGCACCATGGTGCAAAACTTTATCCCGATGGCAACCTATCGCGGTGATGAAGCTTGGCTTGGTGGTTGGACGGT
TTTCTTTTGGGGCTGGTTTTTGGGTTATGGCCCGATGATGGCAATATTTATCGCTCGTATCTCACGCGGTCGCACGATTC
GTGAATTGATTACGACCGTGTGCATTTTGGCGCCATTGGTTACCTGCTTTTGGTTCACGGTCATCGGCGGTTCGGGTCTT
GCGTTTGAGATTGCCAATCCTGGTAGTGTGAGTACAGCATTTGAAGGCTTTAATCTTCCTGGAGCACTGCTTGCCATCAC
TCAGCAGCTGCCATTTCCTTTGATTACTTCCTTATTGTTTATCATTCTAACCATGGTTTTCATTGTTACTACTGGTGATT
CGATGACCTATACCATCAGTGTCGTGATCAGTGGTGAGAGAGAGCCAAATGCTATCATTCGCACCTTTTGGGGCGTGATG
ATGGGGGTGACGGCGATTGTTCTTATCTCGCTTGGTGCAGGCGGCGTGACAGCGTTGCAATCCTTTATTGTCATTACGGC
GGTGCCTGTCTCCTTTGTTTTGCTGCCATCATTATGGAATGGACCAAAAATTGCGCAGCAGATGGCAAGGGATCAAGATC
TTTATCGCCCTAATAGCGTGGAAACTGCTCATCAACTCATCGAAGAGCAGCTTGTCGAAAAAAGTATTGGTAAAGAAGTT
GATTAG

Upstream 100 bases:

>100_bases
AATAAGCATTAAAGTAACAAGCATTAAAATATAGATGGGTAAGACGGTTTAGATAAAACGCCAATTTAAATGACGCAACA
TTATCATATGGAATTACATC

Downstream 100 bases:

>100_bases
ATAGCGCTTTGTTATCGCTGATAGTTTATTTGCTCATAAAAACTGCAAATCTACAGTATTAAAAGGTGGTTGGTTCAACA
CATTATTGAAGGTGATAGTT

Product: BCCT transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MADLKGFNSKQHEQGRSHKTDKIADNLMHVETPSDTLGLKNPAFWYSGGFIIAFVLMAIFAEERLGKLINTGFSWSATVF
GPFWQILLLATFVIALAVGAGRTGRVILGNLAKPEMDGFKWMAILFCTLLAGGGVFWAAAEPIAHFVSAPPLYGESADIQ
QRAFNALSQSFMHWGFLAWSIVGSLTAIVVMHLHYDKGLPLKPRTLLYPMFGERVLTGHTGAIIDACCIVAVAAGTIGPI
GFLGLQTSYALNTLFGIPDTFTTQLIIIIFAIALYTLSAISGLARGMQLLSRFNVILAFGLMVFILMFGPTKFIVNGYIQ
GVGTMVQNFIPMATYRGDEAWLGGWTVFFWGWFLGYGPMMAIFIARISRGRTIRELITTVCILAPLVTCFWFTVIGGSGL
AFEIANPGSVSTAFEGFNLPGALLAITQQLPFPLITSLLFIILTMVFIVTTGDSMTYTISVVISGEREPNAIIRTFWGVM
MGVTAIVLISLGAGGVTALQSFIVITAVPVSFVLLPSLWNGPKIAQQMARDQDLYRPNSVETAHQLIEEQLVEKSIGKEV
D

Sequences:

>Translated_561_residues
MADLKGFNSKQHEQGRSHKTDKIADNLMHVETPSDTLGLKNPAFWYSGGFIIAFVLMAIFAEERLGKLINTGFSWSATVF
GPFWQILLLATFVIALAVGAGRTGRVILGNLAKPEMDGFKWMAILFCTLLAGGGVFWAAAEPIAHFVSAPPLYGESADIQ
QRAFNALSQSFMHWGFLAWSIVGSLTAIVVMHLHYDKGLPLKPRTLLYPMFGERVLTGHTGAIIDACCIVAVAAGTIGPI
GFLGLQTSYALNTLFGIPDTFTTQLIIIIFAIALYTLSAISGLARGMQLLSRFNVILAFGLMVFILMFGPTKFIVNGYIQ
GVGTMVQNFIPMATYRGDEAWLGGWTVFFWGWFLGYGPMMAIFIARISRGRTIRELITTVCILAPLVTCFWFTVIGGSGL
AFEIANPGSVSTAFEGFNLPGALLAITQQLPFPLITSLLFIILTMVFIVTTGDSMTYTISVVISGEREPNAIIRTFWGVM
MGVTAIVLISLGAGGVTALQSFIVITAVPVSFVLLPSLWNGPKIAQQMARDQDLYRPNSVETAHQLIEEQLVEKSIGKEV
D
>Mature_560_residues
ADLKGFNSKQHEQGRSHKTDKIADNLMHVETPSDTLGLKNPAFWYSGGFIIAFVLMAIFAEERLGKLINTGFSWSATVFG
PFWQILLLATFVIALAVGAGRTGRVILGNLAKPEMDGFKWMAILFCTLLAGGGVFWAAAEPIAHFVSAPPLYGESADIQQ
RAFNALSQSFMHWGFLAWSIVGSLTAIVVMHLHYDKGLPLKPRTLLYPMFGERVLTGHTGAIIDACCIVAVAAGTIGPIG
FLGLQTSYALNTLFGIPDTFTTQLIIIIFAIALYTLSAISGLARGMQLLSRFNVILAFGLMVFILMFGPTKFIVNGYIQG
VGTMVQNFIPMATYRGDEAWLGGWTVFFWGWFLGYGPMMAIFIARISRGRTIRELITTVCILAPLVTCFWFTVIGGSGLA
FEIANPGSVSTAFEGFNLPGALLAITQQLPFPLITSLLFIILTMVFIVTTGDSMTYTISVVISGEREPNAIIRTFWGVMM
GVTAIVLISLGAGGVTALQSFIVITAVPVSFVLLPSLWNGPKIAQQMARDQDLYRPNSVETAHQLIEEQLVEKSIGKEVD

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=486, Percent_Identity=30.8641975308642, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1786224, Length=472, Percent_Identity=27.5423728813559, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788102, Length=468, Percent_Identity=27.5641025641026, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60990; Mature: 60858

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLKGFNSKQHEQGRSHKTDKIADNLMHVETPSDTLGLKNPAFWYSGGFIIAFVLMAIF
CCCCCCCCCHHHHCCCCCCHHHHHHHHEECCCCCHHCCCCCCCCEECCHHHHHHHHHHHH
AEERLGKLINTGFSWSATVFGPFWQILLLATFVIALAVGAGRTGRVILGNLAKPEMDGFK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHH
WMAILFCTLLAGGGVFWAAAEPIAHFVSAPPLYGESADIQQRAFNALSQSFMHWGFLAWS
HHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVGSLTAIVVMHLHYDKGLPLKPRTLLYPMFGERVLTGHTGAIIDACCIVAVAAGTIGPI
HHHHHHHHHHHHHCCCCCCCCCCHHEEEHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCH
GFLGLQTSYALNTLFGIPDTFTTQLIIIIFAIALYTLSAISGLARGMQLLSRFNVILAFG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVFILMFGPTKFIVNGYIQGVGTMVQNFIPMATYRGDEAWLGGWTVFFWGWFLGYGPMM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHH
AIFIARISRGRTIRELITTVCILAPLVTCFWFTVIGGSGLAFEIANPGSVSTAFEGFNLP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCC
GALLAITQQLPFPLITSLLFIILTMVFIVTTGDSMTYTISVVISGEREPNAIIRTFWGVM
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHH
MGVTAIVLISLGAGGVTALQSFIVITAVPVSFVLLPSLWNGPKIAQQMARDQDLYRPNSV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCH
ETAHQLIEEQLVEKSIGKEVD
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADLKGFNSKQHEQGRSHKTDKIADNLMHVETPSDTLGLKNPAFWYSGGFIIAFVLMAIF
CCCCCCCCHHHHCCCCCCHHHHHHHHEECCCCCHHCCCCCCCCEECCHHHHHHHHHHHH
AEERLGKLINTGFSWSATVFGPFWQILLLATFVIALAVGAGRTGRVILGNLAKPEMDGFK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHH
WMAILFCTLLAGGGVFWAAAEPIAHFVSAPPLYGESADIQQRAFNALSQSFMHWGFLAWS
HHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVGSLTAIVVMHLHYDKGLPLKPRTLLYPMFGERVLTGHTGAIIDACCIVAVAAGTIGPI
HHHHHHHHHHHHHCCCCCCCCCCHHEEEHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCH
GFLGLQTSYALNTLFGIPDTFTTQLIIIIFAIALYTLSAISGLARGMQLLSRFNVILAFG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMVFILMFGPTKFIVNGYIQGVGTMVQNFIPMATYRGDEAWLGGWTVFFWGWFLGYGPMM
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHH
AIFIARISRGRTIRELITTVCILAPLVTCFWFTVIGGSGLAFEIANPGSVSTAFEGFNLP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCC
GALLAITQQLPFPLITSLLFIILTMVFIVTTGDSMTYTISVVISGEREPNAIIRTFWGVM
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHH
MGVTAIVLISLGAGGVTALQSFIVITAVPVSFVLLPSLWNGPKIAQQMARDQDLYRPNSV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCH
ETAHQLIEEQLVEKSIGKEVD
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]