Click here to switch to the map view.

The map label for this gene is waaA [H]

Identifier: 93006554

GI number: 93006554

Start: 2142887

End: 2144482

Strand: Reverse

Name: waaA [H]

Synonym: Pcryo_1730

Alternate gene names: 93006554

Gene position: 2144482-2142887 (Counterclockwise)

Preceding gene: 93006555

Following gene: 93006553

Centisome position: 70.08

GC content: 46.12

Gene sequence:

>1596_bases
ATGCCATCTTCTGACACCTCAAAAAATCTTTCTATTCAGGCACAGCCTCTGCAAAGCCATGCGGTATATAAGCCACCTGT
GTATTATCAAGTCGGCATAAGCTTATTAAAGCCGTTATATCGCCTGCAAGTATGGCGCCGCTCGCATGCGCGCGATAATT
ATAAGCAAGAAGTTGAACAGCGTTTTGGTAAGCGTTATCCTGCGCCTCCTATAGCCACAGAAACGTATCTGCCAGCTGCG
GATTTAAACAGTAGCATCAATGTTAATAAGCGAAATAAAACCATCTGGTGCCACGCGGTCTCTTTGGGAGAAACCAATAC
CGTTGCGCCATTATTGGATGCACTATTGTCTCGTGGTTATCGGATATGGCTGACCAACACTACCCAGACTGGTTTTGCGC
GCGGTGCCAGTCGATTTGCAGAAGACATTGCGCAAGGTCGATTGAGTCACAGTTATGTGCCGGTTGATAGTCCGGCGGTG
ATTGAGCGCTTTTTGACTCATGTACAACCTGTTGCTGCCTTATTTGTAGAAACAGAGCTATGGGCAAATATTTTAACCAA
ATTATCTGAGCATCATATCCCAAGTATTCTAGTCAATGGTCGACTATCAGCATCCTCTTTTAAAAGCTATCAAAAAATTG
GCGCAGTCAGCACCAGCATGATGAAAAATTTGAGTTTAATCATTGCGCAGGATAATGACTCCGCCAAACGCTTTCGACAA
TTGGGTGCGCATAGCGCCCAAATCCGTGTGGCAGGCTCGCTAAAATGGGTCATTAATACGCCTAAATTGTCTAATGTATA
TGAGCCAGATGATGACATGACTATCGATGTCGATATTGAAAATAGCGAGCAGACAGAGTTAGCAAAAAAAACGAGTGTGA
CGGCGAGTGATTTGGGCGTGGTCAATCGCCCAATTTGGGTGGCAGCAAGCACTCATGGTGGTGAAGAGGATACGGTACTG
TCATGGCAGCAGCAATTACTGTCAAACCCTGCATTGGCAGAGGCGCTACTTATTATCGTCCCACGCCATCCAGAGCGCTT
TGATGAAGTGGCAGCACTGATTCAAAATTCAGGCTTGACCATGGCACGGCGCAGTGCAGCAGAAGCTATCGACATAACTA
CCCAAGTCTATCTGGCGGATAGCATGGGTGAGTTAATGAATTGGTATGCTCTTGCCGATGTGGCATTGGTTGGCGGCTCA
TTGGTCGAGGTGGGCGGACATAATCCAGTTGAGCCGGCAAGTGTGGCGACACCCGTGTTGATGGGTATCCATACGCAATC
TTGCCAAAGCGTCGTGGATAAATTAGCAAGCGTAGGGGCGTTATATCAGCCAAACAACCCTTTTTATCAGGCGATTGATC
ATACTAATAATCATGATGAACAAGCGAACGAAAACCAAAAATTTGCCGATGATGAGACGCTGATTTATCAGCAGCTAAAA
TATTGGATGAGTCATTTAGCATTGGCTAAACAGGCAGGGGAGGCGGGCGCGCAGATGACCGATCAGCAGCAAGCAGTATT
GAGTCGTCAATTATCGATGATAGAAAAAGTCATCGAGCAGCATGCTCTTCAAAGTGTTAGCCAAGATTTAAATTAA

Upstream 100 bases:

>100_bases
AATTTAAAAACGTATTGTTTGGTAATAAATTCAGGTAGAATTGGTGCGTTTTTTGTGCGCGACTCCTATTATCTGCTCCA
TTAATTATCTAGGCTTTTGC

Downstream 100 bases:

>100_bases
GAGCCACTATGAACTGTATTTTATTACCGGCTGCAAACTTTTCTAATGAATCTGCATATATCAATGCAGTGGCGCAAATT
GAGCATGTCAACCAAGTTCT

Product: three-deoxy-D-manno-octulosonic-acid transferase-like

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MPSSDTSKNLSIQAQPLQSHAVYKPPVYYQVGISLLKPLYRLQVWRRSHARDNYKQEVEQRFGKRYPAPPIATETYLPAA
DLNSSINVNKRNKTIWCHAVSLGETNTVAPLLDALLSRGYRIWLTNTTQTGFARGASRFAEDIAQGRLSHSYVPVDSPAV
IERFLTHVQPVAALFVETELWANILTKLSEHHIPSILVNGRLSASSFKSYQKIGAVSTSMMKNLSLIIAQDNDSAKRFRQ
LGAHSAQIRVAGSLKWVINTPKLSNVYEPDDDMTIDVDIENSEQTELAKKTSVTASDLGVVNRPIWVAASTHGGEEDTVL
SWQQQLLSNPALAEALLIIVPRHPERFDEVAALIQNSGLTMARRSAAEAIDITTQVYLADSMGELMNWYALADVALVGGS
LVEVGGHNPVEPASVATPVLMGIHTQSCQSVVDKLASVGALYQPNNPFYQAIDHTNNHDEQANENQKFADDETLIYQQLK
YWMSHLALAKQAGEAGAQMTDQQQAVLSRQLSMIEKVIEQHALQSVSQDLN

Sequences:

>Translated_531_residues
MPSSDTSKNLSIQAQPLQSHAVYKPPVYYQVGISLLKPLYRLQVWRRSHARDNYKQEVEQRFGKRYPAPPIATETYLPAA
DLNSSINVNKRNKTIWCHAVSLGETNTVAPLLDALLSRGYRIWLTNTTQTGFARGASRFAEDIAQGRLSHSYVPVDSPAV
IERFLTHVQPVAALFVETELWANILTKLSEHHIPSILVNGRLSASSFKSYQKIGAVSTSMMKNLSLIIAQDNDSAKRFRQ
LGAHSAQIRVAGSLKWVINTPKLSNVYEPDDDMTIDVDIENSEQTELAKKTSVTASDLGVVNRPIWVAASTHGGEEDTVL
SWQQQLLSNPALAEALLIIVPRHPERFDEVAALIQNSGLTMARRSAAEAIDITTQVYLADSMGELMNWYALADVALVGGS
LVEVGGHNPVEPASVATPVLMGIHTQSCQSVVDKLASVGALYQPNNPFYQAIDHTNNHDEQANENQKFADDETLIYQQLK
YWMSHLALAKQAGEAGAQMTDQQQAVLSRQLSMIEKVIEQHALQSVSQDLN
>Mature_530_residues
PSSDTSKNLSIQAQPLQSHAVYKPPVYYQVGISLLKPLYRLQVWRRSHARDNYKQEVEQRFGKRYPAPPIATETYLPAAD
LNSSINVNKRNKTIWCHAVSLGETNTVAPLLDALLSRGYRIWLTNTTQTGFARGASRFAEDIAQGRLSHSYVPVDSPAVI
ERFLTHVQPVAALFVETELWANILTKLSEHHIPSILVNGRLSASSFKSYQKIGAVSTSMMKNLSLIIAQDNDSAKRFRQL
GAHSAQIRVAGSLKWVINTPKLSNVYEPDDDMTIDVDIENSEQTELAKKTSVTASDLGVVNRPIWVAASTHGGEEDTVLS
WQQQLLSNPALAEALLIIVPRHPERFDEVAALIQNSGLTMARRSAAEAIDITTQVYLADSMGELMNWYALADVALVGGSL
VEVGGHNPVEPASVATPVLMGIHTQSCQSVVDKLASVGALYQPNNPFYQAIDHTNNHDEQANENQKFADDETLIYQQLKY
WMSHLALAKQAGEAGAQMTDQQQAVLSRQLSMIEKVIEQHALQSVSQDLN

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=417, Percent_Identity=36.21103117506, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 58643; Mature: 58512

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSDTSKNLSIQAQPLQSHAVYKPPVYYQVGISLLKPLYRLQVWRRSHARDNYKQEVEQ
CCCCCCCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RFGKRYPAPPIATETYLPAADLNSSINVNKRNKTIWCHAVSLGETNTVAPLLDALLSRGY
HHCCCCCCCCCCCCCCCCHHCCCCCCEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCE
RIWLTNTTQTGFARGASRFAEDIAQGRLSHSYVPVDSPAVIERFLTHVQPVAALFVETEL
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WANILTKLSEHHIPSILVNGRLSASSFKSYQKIGAVSTSMMKNLSLIIAQDNDSAKRFRQ
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
LGAHSAQIRVAGSLKWVINTPKLSNVYEPDDDMTIDVDIENSEQTELAKKTSVTASDLGV
HCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHCCC
VNRPIWVAASTHGGEEDTVLSWQQQLLSNPALAEALLIIVPRHPERFDEVAALIQNSGLT
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHEEEEEECCCCHHHHHHHHHHHCCCCH
MARRSAAEAIDITTQVYLADSMGELMNWYALADVALVGGSLVEVGGHNPVEPASVATPVL
HHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
MGIHTQSCQSVVDKLASVGALYQPNNPFYQAIDHTNNHDEQANENQKFADDETLIYQQLK
HCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHH
YWMSHLALAKQAGEAGAQMTDQQQAVLSRQLSMIEKVIEQHALQSVSQDLN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSSDTSKNLSIQAQPLQSHAVYKPPVYYQVGISLLKPLYRLQVWRRSHARDNYKQEVEQ
CCCCCCCCCEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RFGKRYPAPPIATETYLPAADLNSSINVNKRNKTIWCHAVSLGETNTVAPLLDALLSRGY
HHCCCCCCCCCCCCCCCCHHCCCCCCEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCE
RIWLTNTTQTGFARGASRFAEDIAQGRLSHSYVPVDSPAVIERFLTHVQPVAALFVETEL
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WANILTKLSEHHIPSILVNGRLSASSFKSYQKIGAVSTSMMKNLSLIIAQDNDSAKRFRQ
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
LGAHSAQIRVAGSLKWVINTPKLSNVYEPDDDMTIDVDIENSEQTELAKKTSVTASDLGV
HCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHCCC
VNRPIWVAASTHGGEEDTVLSWQQQLLSNPALAEALLIIVPRHPERFDEVAALIQNSGLT
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHEEEEEECCCCHHHHHHHHHHHCCCCH
MARRSAAEAIDITTQVYLADSMGELMNWYALADVALVGGSLVEVGGHNPVEPASVATPVL
HHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
MGIHTQSCQSVVDKLASVGALYQPNNPFYQAIDHTNNHDEQANENQKFADDETLIYQQLK
HCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHH
YWMSHLALAKQAGEAGAQMTDQQQAVLSRQLSMIEKVIEQHALQSVSQDLN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]