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The map label for this gene is proB [H]

Identifier: 93006506

GI number: 93006506

Start: 2069998

End: 2071170

Strand: Reverse

Name: proB [H]

Synonym: Pcryo_1682

Alternate gene names: 93006506

Gene position: 2071170-2069998 (Counterclockwise)

Preceding gene: 93006507

Following gene: 93006505

Centisome position: 67.69

GC content: 47.14

Gene sequence:

>1173_bases
ATGGCAGTTGGCATAGAGAACACGATAGAAGAAGCAAGGTTTGTTGAGCAAGCTCGTAACTTTGATATTCAGCGCGTTAT
TGTCAAGATTGGCTCATCGTTATTGACCAATAATGGTCGAGGGCTTGATCGAACTGCCATTTACGAATGGGCAAAGCAGA
TCGCGCAACTCCATAAGCAAGGCGTAGAGGTACTGCTCGTATCTTCAGGTGCTGTCGCTGAAGGGGTGGTGCGTATGAAT
CTTGAGGAGCGTCCTAAAAAACTGGCGGCATTACAAGCCTGTGCATCTGTCGGACAGATGGGTTTGATTGAAACCTGGTG
GTCAGCATTGATTCAATATGGTATTCAAAGCTCACAGTTGCTGCTAACGCATGATGATTTGTCTAACCGTAGTCGCTATC
TTAATACTACTGGCGCGCTTACGCAGCTACTAGAATGGCGTGTGCTGCCGGTCATTAATGAAAATGATACCATTACTATT
GATGAGATTAAGTTTGGTGATAACGATACTCTAGGAGCGATGGCAGCGGCGATGGTCAATGCCGACTTGTACATTATCTT
AACTGATCAAGAAGGTGTCTTTACTGACAATCCACGCAACAACCCTCATGCCAGAATGATCCGTCAAGAGCGTGCGATGG
CTGATTATTTATTTGATATTGCAGGTGATGGTGGCAAGTTGGGTCGTGGTGGTATGTTAACCAAAATTCGTGCTGGTCGT
CTTGCTGCTATGGGTGGCTGTCCGACGGTGATTGTGAGTGGCGCTATTGATGATGTGATCACTCGAGTAGTGTCAGGCGA
AGCAGTTGGTACCTTGCTGACCACCAATGATCAAGACAAAATCATTGCTCGCAAACAGTGGCTTGCCGCGCATTTGCGTA
TGTCGGGCTCTTTAATTGTTGATGCAGGTGCAGCAAAAGCGGTGGTCGAACATCAAAAAAGCTTGTTGCCAGTTGGCGTG
TCTGAAGTTCGTGGAGATTTTGATGAAGGCGATGTGGTCGAAATCGTCCATCAGGATACTGGTGAGCGTATCGCCGTTGG
TCAAGTCAACTTTTCTTCTCGCGATGCCTGCCGCGTTGCTCGTGAGCGTACTGAGCAGTTCGATCGTATCCTTGGTAACA
ATGAAGAACGTGTCGTCATGGTACACCGTGATAACTTAGCATTGACGATGTAA

Upstream 100 bases:

>100_bases
ATGGCGTCGAAGTGGTTTACGCCCCTTAATCTGATTGGTCTTTGCACATATTTTTTAGATGTATCCCCATGATTAATTTA
CCATAGCAACAGGAGTTTAT

Downstream 100 bases:

>100_bases
AAAAATTAATATCTATAGAGCTATAAATATCTTGATAATAAGCGCTTTGCTATTCTTTCATTTAACGTAATTTATCCAGT
GTTATATGCGCTTTGGTGAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAVGIENTIEEARFVEQARNFDIQRVIVKIGSSLLTNNGRGLDRTAIYEWAKQIAQLHKQGVEVLLVSSGAVAEGVVRMN
LEERPKKLAALQACASVGQMGLIETWWSALIQYGIQSSQLLLTHDDLSNRSRYLNTTGALTQLLEWRVLPVINENDTITI
DEIKFGDNDTLGAMAAAMVNADLYIILTDQEGVFTDNPRNNPHARMIRQERAMADYLFDIAGDGGKLGRGGMLTKIRAGR
LAAMGGCPTVIVSGAIDDVITRVVSGEAVGTLLTTNDQDKIIARKQWLAAHLRMSGSLIVDAGAAKAVVEHQKSLLPVGV
SEVRGDFDEGDVVEIVHQDTGERIAVGQVNFSSRDACRVARERTEQFDRILGNNEERVVMVHRDNLALTM

Sequences:

>Translated_390_residues
MAVGIENTIEEARFVEQARNFDIQRVIVKIGSSLLTNNGRGLDRTAIYEWAKQIAQLHKQGVEVLLVSSGAVAEGVVRMN
LEERPKKLAALQACASVGQMGLIETWWSALIQYGIQSSQLLLTHDDLSNRSRYLNTTGALTQLLEWRVLPVINENDTITI
DEIKFGDNDTLGAMAAAMVNADLYIILTDQEGVFTDNPRNNPHARMIRQERAMADYLFDIAGDGGKLGRGGMLTKIRAGR
LAAMGGCPTVIVSGAIDDVITRVVSGEAVGTLLTTNDQDKIIARKQWLAAHLRMSGSLIVDAGAAKAVVEHQKSLLPVGV
SEVRGDFDEGDVVEIVHQDTGERIAVGQVNFSSRDACRVARERTEQFDRILGNNEERVVMVHRDNLALTM
>Mature_389_residues
AVGIENTIEEARFVEQARNFDIQRVIVKIGSSLLTNNGRGLDRTAIYEWAKQIAQLHKQGVEVLLVSSGAVAEGVVRMNL
EERPKKLAALQACASVGQMGLIETWWSALIQYGIQSSQLLLTHDDLSNRSRYLNTTGALTQLLEWRVLPVINENDTITID
EIKFGDNDTLGAMAAAMVNADLYIILTDQEGVFTDNPRNNPHARMIRQERAMADYLFDIAGDGGKLGRGGMLTKIRAGRL
AAMGGCPTVIVSGAIDDVITRVVSGEAVGTLLTTNDQDKIIARKQWLAAHLRMSGSLIVDAGAAKAVVEHQKSLLPVGVS
EVRGDFDEGDVVEIVHQDTGERIAVGQVNFSSRDACRVARERTEQFDRILGNNEERVVMVHRDNLALTM

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=302, Percent_Identity=29.4701986754967, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI62912457, Length=300, Percent_Identity=29.6666666666667, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=40.4371584699454, Blast_Score=251, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=32.3529411764706, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=32.8467153284672, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=30.2158273381295, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=31.4285714285714, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21357643, Length=313, Percent_Identity=31.629392971246, Blast_Score=128, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 42687; Mature: 42556

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGIENTIEEARFVEQARNFDIQRVIVKIGSSLLTNNGRGLDRTAIYEWAKQIAQLHKQ
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
GVEVLLVSSGAVAEGVVRMNLEERPKKLAALQACASVGQMGLIETWWSALIQYGIQSSQL
CCEEEEEECCCHHHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEE
LLTHDDLSNRSRYLNTTGALTQLLEWRVLPVINENDTITIDEIKFGDNDTLGAMAAAMVN
EEEECCCCCCHHEECCHHHHHHHHHHEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHC
ADLYIILTDQEGVFTDNPRNNPHARMIRQERAMADYLFDIAGDGGKLGRGGMLTKIRAGR
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
LAAMGGCPTVIVSGAIDDVITRVVSGEAVGTLLTTNDQDKIIARKQWLAAHLRMSGSLIV
EEECCCCCCEEECCHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
DAGAAKAVVEHQKSLLPVGVSEVRGDFDEGDVVEIVHQDTGERIAVGQVNFSSRDACRVA
ECCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHH
RERTEQFDRILGNNEERVVMVHRDNLALTM
HHHHHHHHHHHCCCCCEEEEEEECCEEEEC
>Mature Secondary Structure 
AVGIENTIEEARFVEQARNFDIQRVIVKIGSSLLTNNGRGLDRTAIYEWAKQIAQLHKQ
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
GVEVLLVSSGAVAEGVVRMNLEERPKKLAALQACASVGQMGLIETWWSALIQYGIQSSQL
CCEEEEEECCCHHHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEE
LLTHDDLSNRSRYLNTTGALTQLLEWRVLPVINENDTITIDEIKFGDNDTLGAMAAAMVN
EEEECCCCCCHHEECCHHHHHHHHHHEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHC
ADLYIILTDQEGVFTDNPRNNPHARMIRQERAMADYLFDIAGDGGKLGRGGMLTKIRAGR
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
LAAMGGCPTVIVSGAIDDVITRVVSGEAVGTLLTTNDQDKIIARKQWLAAHLRMSGSLIV
EEECCCCCCEEECCHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
DAGAAKAVVEHQKSLLPVGVSEVRGDFDEGDVVEIVHQDTGERIAVGQVNFSSRDACRVA
ECCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHH
RERTEQFDRILGNNEERVVMVHRDNLALTM
HHHHHHHHHHHCCCCCEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA