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The map label for this gene is hupB [H]

Identifier: 93006477

GI number: 93006477

Start: 2037810

End: 2038082

Strand: Reverse

Name: hupB [H]

Synonym: Pcryo_1653

Alternate gene names: 93006477

Gene position: 2038082-2037810 (Counterclockwise)

Preceding gene: 93006486

Following gene: 93006476

Centisome position: 66.61

GC content: 41.76

Gene sequence:

>273_bases
ATGAATAAGTCAGAATTAATTGATAGCATTGCTGAGAAAAGTGGTCTAAATAAAACTCAAGCTGGCGATGCATTGAACGC
AGTTATGGAAAGCGTTGGCGAAGCTCTAGAAGCTGGCGATAGCATTTCACTAGTTGGTTTTGGTACTTTTAGCGTAAAAG
ACCGTAAAGCACGTACTGGCCGTAACCCTAAGACTGGTGAAGAGCTAAGTATCCCAGCCAGCAAAGTACCAAGCTTTAAA
GCTGGTAAAAACCTAAAAGAACGCTTGAACTAG

Upstream 100 bases:

>100_bases
TATGCATGAGCCAAGTGGTTTTTATTGCTTAAGTGCTCTGAGAGCGATACAATGCTTGGCGTGAGCGTTTTTTTACCCCC
TAAAACTTGAAGGAAATTTT

Downstream 100 bases:

>100_bases
GCCGTATCTATTTAAAGACAGTCGCACCTTATGGCGTGATACACGAATAAGAACGGCGTCTGTTGACGGTAAAGTGATAA
AAAGTGATGGCTCATAACAG

Product: histone-like DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKSELIDSIAEKSGLNKTQAGDALNAVMESVGEALEAGDSISLVGFGTFSVKDRKARTGRNPKTGEELSIPASKVPSFK
AGKNLKERLN

Sequences:

>Translated_90_residues
MNKSELIDSIAEKSGLNKTQAGDALNAVMESVGEALEAGDSISLVGFGTFSVKDRKARTGRNPKTGEELSIPASKVPSFK
AGKNLKERLN
>Mature_90_residues
MNKSELIDSIAEKSGLNKTQAGDALNAVMESVGEALEAGDSISLVGFGTFSVKDRKARTGRNPKTGEELSIPASKVPSFK
AGKNLKERLN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=60, Blast_Score=116, Evalue=3e-28,
Organism=Escherichia coli, GI1790433, Length=87, Percent_Identity=55.1724137931034, Blast_Score=103, Evalue=3e-24,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=41.7582417582418, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=39.3258426966292, Blast_Score=77, Evalue=2e-16,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9508; Mature: 9508

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELIDSIAEKSGLNKTQAGDALNAVMESVGEALEAGDSISLVGFGTFSVKDRKARTG
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCC
RNPKTGEELSIPASKVPSFKAGKNLKERLN
CCCCCCCCCCCCHHHCCCCCCCCCHHHHCC
>Mature Secondary Structure
MNKSELIDSIAEKSGLNKTQAGDALNAVMESVGEALEAGDSISLVGFGTFSVKDRKARTG
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCC
RNPKTGEELSIPASKVPSFKAGKNLKERLN
CCCCCCCCCCCCHHHCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867950; 10984043; 6793399 [H]