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The map label for this gene is leuB [H]

Identifier: 93006395

GI number: 93006395

Start: 1927274

End: 1928377

Strand: Reverse

Name: leuB [H]

Synonym: Pcryo_1571

Alternate gene names: 93006395

Gene position: 1928377-1927274 (Counterclockwise)

Preceding gene: 93006396

Following gene: 93006394

Centisome position: 63.02

GC content: 45.47

Gene sequence:

>1104_bases
ATGGCAACGATTTTAACATTAGCGGGCGATGGTATTGGCCCTGAAATCATGACTCAGGCCATTGATGTGCTGAACGCTGT
CAATGATAAGTTTGCGTTAGGTTTGACCCTTGAGTCTGGATTGATCGGCGGTGTGGCAGTTGATTCAACAGGTGAACCTT
TGCCAGAAGAGACGCTACAACGTGCACGCGCAGCAGATGCAGTGTTATTAGGTGCAGTTGGTGGTCCTAAGTGGGATGGT
ATCGAACGTAGTAAACGTCCTGAGCGCGGTTTGCTAAAAATCCGTAGTGAGCTTGGTTTGTTTGCCAATCTGCGTGTAGC
CAAACTTTATCCGCAGCTTGTGAATGCTTCAAGCATTAAACCTGAGATTATCTCAGGTCTAGATTTGCTGATCGTGCGTG
AATTAACTGGTGGTATTTATTTTGGTGAGCCGCGGGGTATTCGTACACTAGAAAATGGTGAGCAGCAAGGCTATAACACC
ATGGTGTATAGCACTAGCGAAATCAATCGTATTGGCAAAGTTGCTTTTGAATTGGCGCAAACACGCGCGCAAGCAGCAGG
AACCGCTGCCAAAGTCTGCTCTATCGACAAAGCCAATGTATTGGAAGTTACTGAGCTTTGGAAACAGACGATGATTGAAT
TACAGCAGTCAGAGTATAGCGATGTGGCGTTATCTCATATGTATGCTGACAATGCTTGTATGCAGCTGATTAAAGACCCC
AAACAATTCGATGTTATGGTCACTGGCAATCTGTTTGGTGATATCTTATCTGATGAAGCAGCGATGCTGACGGGTTCTAT
TGGCATGCTGCCATCAGCCAGCCTTGATGAAGCGGGCAAAGGCATGTATGAGCCTTGTCATGGTTCAGCGCCTGATATCG
CAGGACAAGATATAGCCAATCCACTAGCGACCATTTTATCGGTTTCTATGATGTTACGTTATACTTTTAAACAAGAAGCA
GCAGCACAAGCGATTGAACAAGCGGTCAGTGATGTGCTTGATGATGGCTTACGTACGGTCGATATCTTGGATCGTAATGA
AGCTGGACTTATACAAGTCGGTTGTCAGCAAATGGGACAAGCCGTATTGGCAAAATTGATTTAA

Upstream 100 bases:

>100_bases
CAAAGTGTAATTATTAAAATCCTATCGTTATTAGCTAAAAACAAAAGCAAGTATAAAATAAGTTATAGATATATAAATTT
ACTCAACCTCAAGGACCAGT

Downstream 100 bases:

>100_bases
TAGAGATTACTTTATCCTAATCTGAATTTTTGAAAATTTATAATAATGAACCCATAATTTGCTAAGCAAGTTATGGGTTT
TTTACTGTTCGCTTTTAGAG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MATILTLAGDGIGPEIMTQAIDVLNAVNDKFALGLTLESGLIGGVAVDSTGEPLPEETLQRARAADAVLLGAVGGPKWDG
IERSKRPERGLLKIRSELGLFANLRVAKLYPQLVNASSIKPEIISGLDLLIVRELTGGIYFGEPRGIRTLENGEQQGYNT
MVYSTSEINRIGKVAFELAQTRAQAAGTAAKVCSIDKANVLEVTELWKQTMIELQQSEYSDVALSHMYADNACMQLIKDP
KQFDVMVTGNLFGDILSDEAAMLTGSIGMLPSASLDEAGKGMYEPCHGSAPDIAGQDIANPLATILSVSMMLRYTFKQEA
AAQAIEQAVSDVLDDGLRTVDILDRNEAGLIQVGCQQMGQAVLAKLI

Sequences:

>Translated_367_residues
MATILTLAGDGIGPEIMTQAIDVLNAVNDKFALGLTLESGLIGGVAVDSTGEPLPEETLQRARAADAVLLGAVGGPKWDG
IERSKRPERGLLKIRSELGLFANLRVAKLYPQLVNASSIKPEIISGLDLLIVRELTGGIYFGEPRGIRTLENGEQQGYNT
MVYSTSEINRIGKVAFELAQTRAQAAGTAAKVCSIDKANVLEVTELWKQTMIELQQSEYSDVALSHMYADNACMQLIKDP
KQFDVMVTGNLFGDILSDEAAMLTGSIGMLPSASLDEAGKGMYEPCHGSAPDIAGQDIANPLATILSVSMMLRYTFKQEA
AAQAIEQAVSDVLDDGLRTVDILDRNEAGLIQVGCQQMGQAVLAKLI
>Mature_366_residues
ATILTLAGDGIGPEIMTQAIDVLNAVNDKFALGLTLESGLIGGVAVDSTGEPLPEETLQRARAADAVLLGAVGGPKWDGI
ERSKRPERGLLKIRSELGLFANLRVAKLYPQLVNASSIKPEIISGLDLLIVRELTGGIYFGEPRGIRTLENGEQQGYNTM
VYSTSEINRIGKVAFELAQTRAQAAGTAAKVCSIDKANVLEVTELWKQTMIELQQSEYSDVALSHMYADNACMQLIKDPK
QFDVMVTGNLFGDILSDEAAMLTGSIGMLPSASLDEAGKGMYEPCHGSAPDIAGQDIANPLATILSVSMMLRYTFKQEAA
AQAIEQAVSDVLDDGLRTVDILDRNEAGLIQVGCQQMGQAVLAKLI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=31.044776119403, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178816, Length=317, Percent_Identity=29.6529968454259, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178821, Length=317, Percent_Identity=29.6529968454259, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4758582, Length=348, Percent_Identity=29.0229885057471, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI28178838, Length=340, Percent_Identity=28.8235294117647, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=157, Percent_Identity=35.031847133758, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87081683, Length=341, Percent_Identity=53.3724340175953, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=349, Percent_Identity=32.9512893982808, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1787381, Length=408, Percent_Identity=26.4705882352941, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=334, Percent_Identity=31.437125748503, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=350, Percent_Identity=26.8571428571429, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17550882, Length=347, Percent_Identity=29.1066282420749, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=347, Percent_Identity=44.0922190201729, Blast_Score=281, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=347, Percent_Identity=35.4466858789625, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=345, Percent_Identity=31.0144927536232, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324291, Length=250, Percent_Identity=30.8, Blast_Score=109, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24643268, Length=368, Percent_Identity=29.3478260869565, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24643270, Length=368, Percent_Identity=29.3478260869565, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24661184, Length=348, Percent_Identity=29.5977011494253, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078635, Length=260, Percent_Identity=28.0769230769231, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078633, Length=260, Percent_Identity=28.0769230769231, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24650122, Length=260, Percent_Identity=28.0769230769231, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078639, Length=262, Percent_Identity=27.8625954198473, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI161078637, Length=260, Percent_Identity=28.0769230769231, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281362242, Length=345, Percent_Identity=25.5072463768116, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648872, Length=345, Percent_Identity=25.5072463768116, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130355, Length=349, Percent_Identity=24.3553008595989, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39201; Mature: 39069

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATILTLAGDGIGPEIMTQAIDVLNAVNDKFALGLTLESGLIGGVAVDSTGEPLPEETLQ
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCHHHHH
RARAADAVLLGAVGGPKWDGIERSKRPERGLLKIRSELGLFANLRVAKLYPQLVNASSIK
HHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCHHHHHHHHHHCCCCCC
PEIISGLDLLIVRELTGGIYFGEPRGIRTLENGEQQGYNTMVYSTSEINRIGKVAFELAQ
HHHHCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
TRAQAAGTAAKVCSIDKANVLEVTELWKQTMIELQQSEYSDVALSHMYADNACMQLIKDP
HHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KQFDVMVTGNLFGDILSDEAAMLTGSIGMLPSASLDEAGKGMYEPCHGSAPDIAGQDIAN
CCEEEEEECHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
PLATILSVSMMLRYTFKQEAAAQAIEQAVSDVLDDGLRTVDILDRNEAGLIQVGCQQMGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
AVLAKLI
HHHHHHC
>Mature Secondary Structure 
ATILTLAGDGIGPEIMTQAIDVLNAVNDKFALGLTLESGLIGGVAVDSTGEPLPEETLQ
CEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCCCHHHHH
RARAADAVLLGAVGGPKWDGIERSKRPERGLLKIRSELGLFANLRVAKLYPQLVNASSIK
HHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCHHHHHHHHHHCCCCCC
PEIISGLDLLIVRELTGGIYFGEPRGIRTLENGEQQGYNTMVYSTSEINRIGKVAFELAQ
HHHHCCHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
TRAQAAGTAAKVCSIDKANVLEVTELWKQTMIELQQSEYSDVALSHMYADNACMQLIKDP
HHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KQFDVMVTGNLFGDILSDEAAMLTGSIGMLPSASLDEAGKGMYEPCHGSAPDIAGQDIAN
CCEEEEEECHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
PLATILSVSMMLRYTFKQEAAAQAIEQAVSDVLDDGLRTVDILDRNEAGLIQVGCQQMGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
AVLAKLI
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA