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The map label for this gene is ilvB [H]

Identifier: 93006373

GI number: 93006373

Start: 1901206

End: 1902948

Strand: Reverse

Name: ilvB [H]

Synonym: Pcryo_1549

Alternate gene names: 93006373

Gene position: 1902948-1901206 (Counterclockwise)

Preceding gene: 93006374

Following gene: 93006372

Centisome position: 62.19

GC content: 48.08

Gene sequence:

>1743_bases
ATGACGCATTCTCTACAAACGCCTTCTTCATTGCCAAATTCCGCGCCAACTGCCTCATCTCTAACTTGCGGTGAGCTGCT
TGTTCAGTGGTTAGAGTATTATGGGGTGGAGACGGTATTTGGTATTCCGGGCGTACATACGGTCGAGCTATATCGTGGCC
TGCCCAATACCGATATTCGCCACGTGACACCGCGTCATGAGCAAGGCGCAGGCTTTATGGCGGATGGTTATTACCGCGCT
TCTGGTAAAGTTGGAGTATGCTTTATCATCACCGGTCCCGGCATGACCAACATCATGACGGCGATGGCACAAGCATTGGC
GGATTCTATACCGATGCTGGTGATATCTAGCGTCAATAAAGTGCAGCATACCGGCAGCGGTGAAGGGCACTTGCATGAAT
TGCACGATCAGCAAGGTATGGTCAGTAAGGTTGCGCTCATGAGTAAAACGATTTGGCAGCCTGAGTCATTACCAAAAGTC
ATCGCTGAAGCTTTTGCGTTATTCAATGGCGCGCGTCCAGGGCCGGTACATATCCAGCTGCCCATTGATGTGATTACCGC
CGATGCCAGTCATGTCGCCAAGCCGCCTAAGCTAAATAAGCTAGATGCTTCTCTAAACAGCCAATCTACTAATAATATTT
CGACGCAACTACAAGCAAACAAACCGCTACCTCATCCTGCGCAATTGGATTTGATTGTTGAGCAGCTTAAAAGCGCAAAA
AATCCAGTATTGCTCTATGGCGGTGGCTGCGTTGATGTTAATCATGACGCACAAAAATTAGCAGAACTTATCGATGCACC
GACGTTTTTGACCATCAATGCCAAAGGTTTACTGCCGCCTGCTCATCCGCTGAGTTTGGGTAGCAATCAGTCATTGGATG
TTGGTCGCGCTGTCATTAGCGAGGCCGACTTAGTATTGGCGATTGGTACTGAGCTTGGCGAGACTGATTATGACGTCTTT
TTTAATGGTGGGTTCCAAATCAATGGCACGCTGATTCGTATCGACTGCGATGCTCAGCAATTACAGCGTCCGTTTAGAGC
AGATATTGCGCTATTGTCAGATGCGCAAATGGCTATCAGCGGTTTATGTACGCGCTTAGAAAATCAACCGCTGAATCATC
AAGCCGCACCGCGAGTAGCAAAGGTAAAGCAAGCGTTGATAGAAGAAATGACGCCAAATTTTGCTGGTCAAAACGCACTG
CTACAGCTGATTCGAGATGAAGTAAAAGACGTCATTTTCGTTGGCGATTCAACCCAGCCTGTTTATAGTGGCAACCTTGG
TTTTGAGGCTTTAGCCACGCGTCGATGGTTTAACTCATCGACTGGCTTTGGCACCTTGGGTTATGGTTTGCCTGCCGCGA
TTGGTGCCATGATTGGCTCAAAAGCGCCGGTTGTGAGTCTGATTGGTGATGGCGGCATTCAGTTTACCATCACAGAACTA
ATCTGCGCCGCCGAGCTTGATCTACCATTGATTGTGCTGTTATGGAACAACCAAGGTTACGGTGAGATTCGCCGCTATAT
GGAAGAGGGCGGCCTGCCACTGATCGGGGTCAATATTAAAACCCCGAACTTTGAGCCATTAGCCGCAGGATTTGGTGCCG
GTTATCGTCGAATCACAGATAAGCAGCAGCTGCTCGAGGCATTAAAAGAAGACATCAAAGGCAAACAACCGATTATTTAT
GAGGTTGATGAAGCCGATGAGTTTCTTCTTGAAATGGGCAAGAGTTTTACCTGTTTTAGCTGA

Upstream 100 bases:

>100_bases
TGTCCTTTATAGGGAATTAAAGGGCAGTTTGGGTTTAGCTTGGATTTGATATTGAACGACTTATTTAATTGCTATTGAAT
TTTTTTAGGATGATTAACTC

Downstream 100 bases:

>100_bases
TGTAATCAAGCATCATTTACATGAAAAATAACTAATAAAACGGCATTCAACTATTTGCTCAATTCGCTAAAACTAGGGAT
AGGTCAGACAGCGAAGGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MTHSLQTPSSLPNSAPTASSLTCGELLVQWLEYYGVETVFGIPGVHTVELYRGLPNTDIRHVTPRHEQGAGFMADGYYRA
SGKVGVCFIITGPGMTNIMTAMAQALADSIPMLVISSVNKVQHTGSGEGHLHELHDQQGMVSKVALMSKTIWQPESLPKV
IAEAFALFNGARPGPVHIQLPIDVITADASHVAKPPKLNKLDASLNSQSTNNISTQLQANKPLPHPAQLDLIVEQLKSAK
NPVLLYGGGCVDVNHDAQKLAELIDAPTFLTINAKGLLPPAHPLSLGSNQSLDVGRAVISEADLVLAIGTELGETDYDVF
FNGGFQINGTLIRIDCDAQQLQRPFRADIALLSDAQMAISGLCTRLENQPLNHQAAPRVAKVKQALIEEMTPNFAGQNAL
LQLIRDEVKDVIFVGDSTQPVYSGNLGFEALATRRWFNSSTGFGTLGYGLPAAIGAMIGSKAPVVSLIGDGGIQFTITEL
ICAAELDLPLIVLLWNNQGYGEIRRYMEEGGLPLIGVNIKTPNFEPLAAGFGAGYRRITDKQQLLEALKEDIKGKQPIIY
EVDEADEFLLEMGKSFTCFS

Sequences:

>Translated_580_residues
MTHSLQTPSSLPNSAPTASSLTCGELLVQWLEYYGVETVFGIPGVHTVELYRGLPNTDIRHVTPRHEQGAGFMADGYYRA
SGKVGVCFIITGPGMTNIMTAMAQALADSIPMLVISSVNKVQHTGSGEGHLHELHDQQGMVSKVALMSKTIWQPESLPKV
IAEAFALFNGARPGPVHIQLPIDVITADASHVAKPPKLNKLDASLNSQSTNNISTQLQANKPLPHPAQLDLIVEQLKSAK
NPVLLYGGGCVDVNHDAQKLAELIDAPTFLTINAKGLLPPAHPLSLGSNQSLDVGRAVISEADLVLAIGTELGETDYDVF
FNGGFQINGTLIRIDCDAQQLQRPFRADIALLSDAQMAISGLCTRLENQPLNHQAAPRVAKVKQALIEEMTPNFAGQNAL
LQLIRDEVKDVIFVGDSTQPVYSGNLGFEALATRRWFNSSTGFGTLGYGLPAAIGAMIGSKAPVVSLIGDGGIQFTITEL
ICAAELDLPLIVLLWNNQGYGEIRRYMEEGGLPLIGVNIKTPNFEPLAAGFGAGYRRITDKQQLLEALKEDIKGKQPIIY
EVDEADEFLLEMGKSFTCFS
>Mature_579_residues
THSLQTPSSLPNSAPTASSLTCGELLVQWLEYYGVETVFGIPGVHTVELYRGLPNTDIRHVTPRHEQGAGFMADGYYRAS
GKVGVCFIITGPGMTNIMTAMAQALADSIPMLVISSVNKVQHTGSGEGHLHELHDQQGMVSKVALMSKTIWQPESLPKVI
AEAFALFNGARPGPVHIQLPIDVITADASHVAKPPKLNKLDASLNSQSTNNISTQLQANKPLPHPAQLDLIVEQLKSAKN
PVLLYGGGCVDVNHDAQKLAELIDAPTFLTINAKGLLPPAHPLSLGSNQSLDVGRAVISEADLVLAIGTELGETDYDVFF
NGGFQINGTLIRIDCDAQQLQRPFRADIALLSDAQMAISGLCTRLENQPLNHQAAPRVAKVKQALIEEMTPNFAGQNALL
QLIRDEVKDVIFVGDSTQPVYSGNLGFEALATRRWFNSSTGFGTLGYGLPAAIGAMIGSKAPVVSLIGDGGIQFTITELI
CAAELDLPLIVLLWNNQGYGEIRRYMEEGGLPLIGVNIKTPNFEPLAAGFGAGYRRITDKQQLLEALKEDIKGKQPIIYE
VDEADEFLLEMGKSFTCFS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=573, Percent_Identity=26.0034904013962, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=21.2121212121212, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI1790104, Length=570, Percent_Identity=29.8245614035088, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=29.7101449275362, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786717, Length=503, Percent_Identity=28.4294234592445, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=25.044404973357, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1787096, Length=577, Percent_Identity=21.8370883882149, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17542570, Length=583, Percent_Identity=24.8713550600343, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=496, Percent_Identity=24.7983870967742, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=496, Percent_Identity=24.7983870967742, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=28.0068728522337, Blast_Score=183, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6320816, Length=537, Percent_Identity=24.2085661080074, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=515, Percent_Identity=23.3009708737864, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=518, Percent_Identity=23.7451737451737, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=518, Percent_Identity=22.972972972973, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320588, Length=551, Percent_Identity=20.8711433756806, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=22.6785714285714, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62437; Mature: 62306

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSLQTPSSLPNSAPTASSLTCGELLVQWLEYYGVETVFGIPGVHTVELYRGLPNTDIR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCEEHHHHCCCCCCCCC
HVTPRHEQGAGFMADGYYRASGKVGVCFIITGPGMTNIMTAMAQALADSIPMLVISSVNK
CCCCCCCCCCCEEECCEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHH
VQHTGSGEGHLHELHDQQGMVSKVALMSKTIWQPESLPKVIAEAFALFNGARPGPVHIQL
HHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
PIDVITADASHVAKPPKLNKLDASLNSQSTNNISTQLQANKPLPHPAQLDLIVEQLKSAK
EEEEEECCHHHCCCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
NPVLLYGGGCVDVNHDAQKLAELIDAPTFLTINAKGLLPPAHPLSLGSNQSLDVGRAVIS
CCEEEECCCEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHC
EADLVLAIGTELGETDYDVFFNGGFQINGTLIRIDCDAQQLQRPFRADIALLSDAQMAIS
CCCEEEEECCCCCCCCCEEEECCCEEECCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHH
GLCTRLENQPLNHQAAPRVAKVKQALIEEMTPNFAGQNALLQLIRDEVKDVIFVGDSTQP
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCC
VYSGNLGFEALATRRWFNSSTGFGTLGYGLPAAIGAMIGSKAPVVSLIGDGGIQFTITEL
EEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEHHHH
ICAAELDLPLIVLLWNNQGYGEIRRYMEEGGLPLIGVNIKTPNFEPLAAGFGAGYRRITD
HHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCHHHCCC
KQQLLEALKEDIKGKQPIIYEVDEADEFLLEMGKSFTCFS
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
THSLQTPSSLPNSAPTASSLTCGELLVQWLEYYGVETVFGIPGVHTVELYRGLPNTDIR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCEEHHHHCCCCCCCCC
HVTPRHEQGAGFMADGYYRASGKVGVCFIITGPGMTNIMTAMAQALADSIPMLVISSVNK
CCCCCCCCCCCEEECCEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHH
VQHTGSGEGHLHELHDQQGMVSKVALMSKTIWQPESLPKVIAEAFALFNGARPGPVHIQL
HHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
PIDVITADASHVAKPPKLNKLDASLNSQSTNNISTQLQANKPLPHPAQLDLIVEQLKSAK
EEEEEECCHHHCCCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
NPVLLYGGGCVDVNHDAQKLAELIDAPTFLTINAKGLLPPAHPLSLGSNQSLDVGRAVIS
CCEEEECCCEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHC
EADLVLAIGTELGETDYDVFFNGGFQINGTLIRIDCDAQQLQRPFRADIALLSDAQMAIS
CCCEEEEECCCCCCCCCEEEECCCEEECCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHH
GLCTRLENQPLNHQAAPRVAKVKQALIEEMTPNFAGQNALLQLIRDEVKDVIFVGDSTQP
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCC
VYSGNLGFEALATRRWFNSSTGFGTLGYGLPAAIGAMIGSKAPVVSLIGDGGIQFTITEL
EEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEHHHH
ICAAELDLPLIVLLWNNQGYGEIRRYMEEGGLPLIGVNIKTPNFEPLAAGFGAGYRRITD
HHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCHHHCCC
KQQLLEALKEDIKGKQPIIYEVDEADEFLLEMGKSFTCFS
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]