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The map label for this gene is yafC [H]

Identifier: 93006367

GI number: 93006367

Start: 1895032

End: 1895937

Strand: Reverse

Name: yafC [H]

Synonym: Pcryo_1541

Alternate gene names: 93006367

Gene position: 1895937-1895032 (Counterclockwise)

Preceding gene: 93006372

Following gene: 93006362

Centisome position: 61.96

GC content: 43.6

Gene sequence:

>906_bases
ATGGGACAATTAGAAGACATGGCAATGTTTGTACGCATTGTTGAAGCCGGTAGCATTACCAAGGCTGCCGAACAACTAAA
TATTGCCAAATCAGCGGTTAGCCGTCGCCTCAAGGATTTAGAGACGCGGTTAGGCAGTCAACTTATTAGCCGCACCACGC
GCCAATCCAACTTAACCCAAGCCGGTGAGCAGTATTACCAAAAGGTGAACAATATACTGAGCGAAGTTGACGCCCTAAAC
GAAGAAACCAGTGGTGCGCCTACGCGTATCGAGGGCATCTTAAAAATGACCGCGCCATTGTCATTTGGCTTGATGCATCT
AAATGATGTCATTGATGAATATGCCAATCAGCATCCCAATTTGAACTTTGAGCTCGATTTCTCTGATCGGCATACAGATT
TGGTTGAAGAAGGGTTTGAATTGGGGATTCGTATTAGAGAACTGCAGGACTCGAGCTATCAAGCCAAACGTCTGGCGCTA
ATTCGCTATACCTTATGTGCCAGCCCTGAATATTTGAGTAAAATGGGCACACCAACTACCCTAGAAGATTTTGCCAAGCA
TGAGTTTTTACAATACGGTCTAAACAAATCCAGCACGATAGAGCTCATAGACGAGCAAGGTAAAAAGCACCAAGTCGCTA
TCAACTCAAAGATAAAGGTCAATAATGGCGACTTTTTACGAGAAATGGCGGTAAAAGGTCATGGAATCGCTTTTTTGCCC
AATTTTATTACTTACAAAACCTTAGCCAGTGGTGCACTTGTGCCCATCATGCAGCAGTATCAATTGCCGACTTTAAATGC
TTATGCTGTTTATCCCAAAAACCGATTTTTATCGCAGCGCTGCCGTTATCTAATCGATTTTATCGCTGAGCGTTTTGGCG
ATGATCCTTATTGGGATCGTTTTTAA

Upstream 100 bases:

>100_bases
CTAAGTAATGATTATATTTTAATTGTTGACCATGTATAGATAAACCGTAATAATCGCAAATAACAGTTCTTATTTAGAGA
ACAGTCAAGGAGCATCTTAT

Downstream 100 bases:

>100_bases
CTTTGTTTATGCGTTGGTGTTTGTTTTGGCTAAGTTTGTAATGCCTGTATAGATTCGCTTAGAGCAACTTAAAAGGTGTT
AAATACTTGCCAATGGCACT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MGQLEDMAMFVRIVEAGSITKAAEQLNIAKSAVSRRLKDLETRLGSQLISRTTRQSNLTQAGEQYYQKVNNILSEVDALN
EETSGAPTRIEGILKMTAPLSFGLMHLNDVIDEYANQHPNLNFELDFSDRHTDLVEEGFELGIRIRELQDSSYQAKRLAL
IRYTLCASPEYLSKMGTPTTLEDFAKHEFLQYGLNKSSTIELIDEQGKKHQVAINSKIKVNNGDFLREMAVKGHGIAFLP
NFITYKTLASGALVPIMQQYQLPTLNAYAVYPKNRFLSQRCRYLIDFIAERFGDDPYWDRF

Sequences:

>Translated_301_residues
MGQLEDMAMFVRIVEAGSITKAAEQLNIAKSAVSRRLKDLETRLGSQLISRTTRQSNLTQAGEQYYQKVNNILSEVDALN
EETSGAPTRIEGILKMTAPLSFGLMHLNDVIDEYANQHPNLNFELDFSDRHTDLVEEGFELGIRIRELQDSSYQAKRLAL
IRYTLCASPEYLSKMGTPTTLEDFAKHEFLQYGLNKSSTIELIDEQGKKHQVAINSKIKVNNGDFLREMAVKGHGIAFLP
NFITYKTLASGALVPIMQQYQLPTLNAYAVYPKNRFLSQRCRYLIDFIAERFGDDPYWDRF
>Mature_300_residues
GQLEDMAMFVRIVEAGSITKAAEQLNIAKSAVSRRLKDLETRLGSQLISRTTRQSNLTQAGEQYYQKVNNILSEVDALNE
ETSGAPTRIEGILKMTAPLSFGLMHLNDVIDEYANQHPNLNFELDFSDRHTDLVEEGFELGIRIRELQDSSYQAKRLALI
RYTLCASPEYLSKMGTPTTLEDFAKHEFLQYGLNKSSTIELIDEQGKKHQVAINSKIKVNNGDFLREMAVKGHGIAFLPN
FITYKTLASGALVPIMQQYQLPTLNAYAVYPKNRFLSQRCRYLIDFIAERFGDDPYWDRF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=35.3741496598639, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=29.6551724137931, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=28.6689419795222, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=28.0821917808219, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=28.3276450511945, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=27.3356401384083, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=23.6111111111111, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=25.4901960784314, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=23.3108108108108, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=25.7692307692308, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=125, Percent_Identity=31.2, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=25, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788748, Length=319, Percent_Identity=25.0783699059561, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34262; Mature: 34131

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQLEDMAMFVRIVEAGSITKAAEQLNIAKSAVSRRLKDLETRLGSQLISRTTRQSNLTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEQYYQKVNNILSEVDALNEETSGAPTRIEGILKMTAPLSFGLMHLNDVIDEYANQHPN
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LNFELDFSDRHTDLVEEGFELGIRIRELQDSSYQAKRLALIRYTLCASPEYLSKMGTPTT
CEEEECCCCCHHHHHHHHHHHCEEHEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCC
LEDFAKHEFLQYGLNKSSTIELIDEQGKKHQVAINSKIKVNNGDFLREMAVKGHGIAFLP
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCEEEECCCHHHHHHHHCCCCEEECC
NFITYKTLASGALVPIMQQYQLPTLNAYAVYPKNRFLSQRCRYLIDFIAERFGDDPYWDR
HHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
F
C
>Mature Secondary Structure 
GQLEDMAMFVRIVEAGSITKAAEQLNIAKSAVSRRLKDLETRLGSQLISRTTRQSNLTQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEQYYQKVNNILSEVDALNEETSGAPTRIEGILKMTAPLSFGLMHLNDVIDEYANQHPN
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LNFELDFSDRHTDLVEEGFELGIRIRELQDSSYQAKRLALIRYTLCASPEYLSKMGTPTT
CEEEECCCCCHHHHHHHHHHHCEEHEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCC
LEDFAKHEFLQYGLNKSSTIELIDEQGKKHQVAINSKIKVNNGDFLREMAVKGHGIAFLP
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCEEEECCCHHHHHHHHCCCCEEECC
NFITYKTLASGALVPIMQQYQLPTLNAYAVYPKNRFLSQRCRYLIDFIAERFGDDPYWDR
HHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]