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The map label for this gene is ksgA [H]

Identifier: 93006282

GI number: 93006282

Start: 1795890

End: 1796753

Strand: Reverse

Name: ksgA [H]

Synonym: Pcryo_1456

Alternate gene names: 93006282

Gene position: 1796753-1795890 (Counterclockwise)

Preceding gene: 93006284

Following gene: 93006281

Centisome position: 58.72

GC content: 45.6

Gene sequence:

>864_bases
ATGTCAAAAACTACATTTGATGCTCAATCTATTACCAACAGCCTACGTGCTGCCAAACACCAACCGCGTAAACGCTTTGG
TCAAAACTTCTTACATGACCGTAGTGTTATCCGTGAGATTGTTGAGAGCATTCGCTTAGAGCGTGATGACAATTTGATTG
AGATTGGGCCAGGGATGGGCGCACTGACTGAGCCGTTATTGGCAGAAGTAGATGCGATGACTGTAGTCGAGTTGGATCGT
GATTTGGCAGATAGCTTGCGTATCCGTATCGGGGCTAATAGTCATCCAAATTTTGAGATTATCAAAAACAATGCCATGCA
TGTGGATTATCGGGAGCTTTATAGCGATGAGCGCGGCAAATTACGCGTGGTGGGTAATCTACCGTATAACATCTCAACGC
CCATACTCTTTCACTTACTCAGCTATGCCGATGTGATTGAAGACATGCACTTTATGCTGCAAAAAGAAGTGGTTGAACGT
ATCACCGCTGACGTCGGTAGCAAAACCTATGGTCGCCTATCGGTCATTATGCAGTACCACTGCCATACGGATTATTTGCT
CACCGTACCACGTGGTGCTTTTAATCCGCCGCCAAAAGTGACCAGTGCCGTATTTCGTCTGACGCCGCACATTATTAAGC
CAGTCGTGGCAGAAGATGAAGAGTATTTTGCATTGGTAGTGCGCGAAACCTTTAATCATCGCCGTAAAACGTTGCGCGCT
ATATTCAAAAAATCAACGTTATTGCCAACGTTGAGCGAAGACGATTTTGCAGCTTGCGCCATTGATCCACAAGCCCGTCC
TGAAACCTTAAGCGTGAAAGATTTTGTCAATTTGAGCAATCAAGCGCGTAAAGTAGAAGGCTGA

Upstream 100 bases:

>100_bases
AAACACAGTCAAATAATAAGCTGTGATATAATTCATCCCAATAAAACACTCATTTTATCGTCATTGCTCACTTTTACCTC
TTACTCATTAAGACTATTCT

Downstream 100 bases:

>100_bases
TTTATCAGTGCTATTCATACCGTAGATATCATTCATTGCTAAGTAGTATCTACAATATTCACAAGAAAGATTGAGAGCTT
ATGAGTTATCGTCATCAGTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSKTTFDAQSITNSLRAAKHQPRKRFGQNFLHDRSVIREIVESIRLERDDNLIEIGPGMGALTEPLLAEVDAMTVVELDR
DLADSLRIRIGANSHPNFEIIKNNAMHVDYRELYSDERGKLRVVGNLPYNISTPILFHLLSYADVIEDMHFMLQKEVVER
ITADVGSKTYGRLSVIMQYHCHTDYLLTVPRGAFNPPPKVTSAVFRLTPHIIKPVVAEDEEYFALVVRETFNHRRKTLRA
IFKKSTLLPTLSEDDFAACAIDPQARPETLSVKDFVNLSNQARKVEG

Sequences:

>Translated_287_residues
MSKTTFDAQSITNSLRAAKHQPRKRFGQNFLHDRSVIREIVESIRLERDDNLIEIGPGMGALTEPLLAEVDAMTVVELDR
DLADSLRIRIGANSHPNFEIIKNNAMHVDYRELYSDERGKLRVVGNLPYNISTPILFHLLSYADVIEDMHFMLQKEVVER
ITADVGSKTYGRLSVIMQYHCHTDYLLTVPRGAFNPPPKVTSAVFRLTPHIIKPVVAEDEEYFALVVRETFNHRRKTLRA
IFKKSTLLPTLSEDDFAACAIDPQARPETLSVKDFVNLSNQARKVEG
>Mature_286_residues
SKTTFDAQSITNSLRAAKHQPRKRFGQNFLHDRSVIREIVESIRLERDDNLIEIGPGMGALTEPLLAEVDAMTVVELDRD
LADSLRIRIGANSHPNFEIIKNNAMHVDYRELYSDERGKLRVVGNLPYNISTPILFHLLSYADVIEDMHFMLQKEVVERI
TADVGSKTYGRLSVIMQYHCHTDYLLTVPRGAFNPPPKVTSAVFRLTPHIIKPVVAEDEEYFALVVRETFNHRRKTLRAI
FKKSTLLPTLSEDDFAACAIDPQARPETLSVKDFVNLSNQARKVEG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=298, Percent_Identity=29.8657718120805, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI7657198, Length=249, Percent_Identity=32.1285140562249, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=47.5095785440613, Blast_Score=238, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI25146882, Length=251, Percent_Identity=30.6772908366534, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25141369, Length=290, Percent_Identity=27.2413793103448, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=259, Percent_Identity=27.4131274131274, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21358017, Length=256, Percent_Identity=32.03125, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21357273, Length=288, Percent_Identity=28.8194444444444, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32644; Mature: 32513

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTTFDAQSITNSLRAAKHQPRKRFGQNFLHDRSVIREIVESIRLERDDNLIEIGPGMG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
ALTEPLLAEVDAMTVVELDRDLADSLRIRIGANSHPNFEIIKNNAMHVDYRELYSDERGK
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCEEEEHHHHHCCCCCC
LRVVGNLPYNISTPILFHLLSYADVIEDMHFMLQKEVVERITADVGSKTYGRLSVIMQYH
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
CHTDYLLTVPRGAFNPPPKVTSAVFRLTPHIIKPVVAEDEEYFALVVRETFNHRRKTLRA
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IFKKSTLLPTLSEDDFAACAIDPQARPETLSVKDFVNLSNQARKVEG
HHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKTTFDAQSITNSLRAAKHQPRKRFGQNFLHDRSVIREIVESIRLERDDNLIEIGPGMG
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
ALTEPLLAEVDAMTVVELDRDLADSLRIRIGANSHPNFEIIKNNAMHVDYRELYSDERGK
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCEEEEHHHHHCCCCCC
LRVVGNLPYNISTPILFHLLSYADVIEDMHFMLQKEVVERITADVGSKTYGRLSVIMQYH
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
CHTDYLLTVPRGAFNPPPKVTSAVFRLTPHIIKPVVAEDEEYFALVVRETFNHRRKTLRA
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IFKKSTLLPTLSEDDFAACAIDPQARPETLSVKDFVNLSNQARKVEG
HHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA