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The map label for this gene is glnA [H]

Identifier: 93006266

GI number: 93006266

Start: 1771506

End: 1772915

Strand: Reverse

Name: glnA [H]

Synonym: Pcryo_1440

Alternate gene names: 93006266

Gene position: 1772915-1771506 (Counterclockwise)

Preceding gene: 93006272

Following gene: 93006265

Centisome position: 57.94

GC content: 44.11

Gene sequence:

>1410_bases
ATGTCAAATAAACTACTTGATCTGATCAAATCATCCAACGCTAAATGGGTTGATTTCCGCTTCACTGATACCCGCGGTAA
AGAGCATCATATGAGCTTTCCAGCTCATAGCGTTGATGAAGAATCGATGGAAGATGGCAAAATGTTTGATGGCTCATCGA
TTGCTGGTTGGAAAGGGATTGAAGCATCAGACATGATATTGCGTCCTGATCCTGATACGGCGTTTATTGACCCATTCTTT
GATGCGGTTACGGTTGTTGTGACTTGTGACATCATTGAGCCATCAACCCTACAAGGTTATGACCGTGATCCACGTTCTAT
TGCGCGCCGTGCTGAAGAGTATTTAAAATCTACCGGTATTGGTGACACTGCCTATTTTGGTCCTGAGCCTGAATTCTTTG
TGTTTGATGAAGTAAAATGGTCTGTGGGCATGTCTGGTGTCAGTCATGAAATCGTCGCTGAAGAAGCCGCTTGGTCAACT
AACAAAGACTACGAGTGGGGCAACATGGGTCATCGCCCACGTGTCAAAGGCGGTTATGTACCAGTGCCACCGGTTGATAG
CGCACAAGATATGCGTACCGTCATGTGTGATCGCATTGAAGATATGATTGGTGAAGGTTGTATCGAAGTCCATCACCATG
AAGTTGCGCCTTGTCAGTTAGAGATTGGCGTTGCTTTTAACACCCTAGTACGCAAAGCAGATGAAGTGCAAAAACTCAAA
TATGCCGTACATAATGTTGCTCATCAGTTTGGAAAAACCGCAACCTTTATGCCAAAGCCTATCGTTGGTGATAATGGTTC
TGGTATGCACGTCCATATCTCAATCTCAAAAGATGGTGTCAATACTTTCTCTGGTGATGAATATGCGGGCTTGTCTGAAA
CGGCATTGTATTTTGTCGGTGGTATCATTAAACACGCTCGTGCACTTAATGCGATTACCAATCCATCAACCAACAGCTAT
AAGCGCCTTGTGCCACATTATGAAGCGCCTATCAAGCTTGCGTACTCAGCATCTAACCGTTCAGCGTCTATCCGTATTCC
GCACGTTAGTAGCCCAAAAGCAGTGCGTGTTGAAGCACGTTTCCCTGATCCAGCAGCCAACCCATATCTAGCGTTTGCGG
CGTTGTTGATGGCTGGTATTGACGGTATTCAAAACAAAATGCATCCAGGTGAAGCAGCTGACAAAAATTTATATGATTTG
CCACCAGAAGAAGAAGCACTAATCCCAACGGTCGCTGAAAACTTAGAAGTGGCGCTACAAGCACTAAAAGATGATCATGA
GTTCTTATTAAAAGGCGATGTATTCACTAAAGAGATGTTAGAGGCTTATATTGCTCTAAAACAAGAAGAAGCACAGCGCG
TCAACATTACTGTTCATCCAGTTGAGTTTGACTTGTATTACAGCTGCTAA

Upstream 100 bases:

>100_bases
ATAACGAGTAAAAATCGTTATCTTCAACCTCAGCACTGTTTAGTAATACAAAGCCATTACTATAATTTCAGTACCATGAT
AATCACTAGGGAAACTATCT

Downstream 100 bases:

>100_bases
TTTATAGCTGATACGCTCAATGATTTTAATAAAAAACCAGTTAAGTAGATCTTAGCTGGTTTTTTTTTGCTCTTGAATAT
GTGTGTTGACTGAGCTGACA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSNKLLDLIKSSNAKWVDFRFTDTRGKEHHMSFPAHSVDEESMEDGKMFDGSSIAGWKGIEASDMILRPDPDTAFIDPFF
DAVTVVVTCDIIEPSTLQGYDRDPRSIARRAEEYLKSTGIGDTAYFGPEPEFFVFDEVKWSVGMSGVSHEIVAEEAAWST
NKDYEWGNMGHRPRVKGGYVPVPPVDSAQDMRTVMCDRIEDMIGEGCIEVHHHEVAPCQLEIGVAFNTLVRKADEVQKLK
YAVHNVAHQFGKTATFMPKPIVGDNGSGMHVHISISKDGVNTFSGDEYAGLSETALYFVGGIIKHARALNAITNPSTNSY
KRLVPHYEAPIKLAYSASNRSASIRIPHVSSPKAVRVEARFPDPAANPYLAFAALLMAGIDGIQNKMHPGEAADKNLYDL
PPEEEALIPTVAENLEVALQALKDDHEFLLKGDVFTKEMLEAYIALKQEEAQRVNITVHPVEFDLYYSC

Sequences:

>Translated_469_residues
MSNKLLDLIKSSNAKWVDFRFTDTRGKEHHMSFPAHSVDEESMEDGKMFDGSSIAGWKGIEASDMILRPDPDTAFIDPFF
DAVTVVVTCDIIEPSTLQGYDRDPRSIARRAEEYLKSTGIGDTAYFGPEPEFFVFDEVKWSVGMSGVSHEIVAEEAAWST
NKDYEWGNMGHRPRVKGGYVPVPPVDSAQDMRTVMCDRIEDMIGEGCIEVHHHEVAPCQLEIGVAFNTLVRKADEVQKLK
YAVHNVAHQFGKTATFMPKPIVGDNGSGMHVHISISKDGVNTFSGDEYAGLSETALYFVGGIIKHARALNAITNPSTNSY
KRLVPHYEAPIKLAYSASNRSASIRIPHVSSPKAVRVEARFPDPAANPYLAFAALLMAGIDGIQNKMHPGEAADKNLYDL
PPEEEALIPTVAENLEVALQALKDDHEFLLKGDVFTKEMLEAYIALKQEEAQRVNITVHPVEFDLYYSC
>Mature_468_residues
SNKLLDLIKSSNAKWVDFRFTDTRGKEHHMSFPAHSVDEESMEDGKMFDGSSIAGWKGIEASDMILRPDPDTAFIDPFFD
AVTVVVTCDIIEPSTLQGYDRDPRSIARRAEEYLKSTGIGDTAYFGPEPEFFVFDEVKWSVGMSGVSHEIVAEEAAWSTN
KDYEWGNMGHRPRVKGGYVPVPPVDSAQDMRTVMCDRIEDMIGEGCIEVHHHEVAPCQLEIGVAFNTLVRKADEVQKLKY
AVHNVAHQFGKTATFMPKPIVGDNGSGMHVHISISKDGVNTFSGDEYAGLSETALYFVGGIIKHARALNAITNPSTNSYK
RLVPHYEAPIKLAYSASNRSASIRIPHVSSPKAVRVEARFPDPAANPYLAFAALLMAGIDGIQNKMHPGEAADKNLYDLP
PEEEALIPTVAENLEVALQALKDDHEFLLKGDVFTKEMLEAYIALKQEEAQRVNITVHPVEFDLYYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=475, Percent_Identity=23.7894736842105, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790301, Length=466, Percent_Identity=65.6652360515021, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=405, Percent_Identity=23.9506172839506, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51984; Mature: 51853

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKLLDLIKSSNAKWVDFRFTDTRGKEHHMSFPAHSVDEESMEDGKMFDGSSIAGWKGI
CCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCC
EASDMILRPDPDTAFIDPFFDAVTVVVTCDIIEPSTLQGYDRDPRSIARRAEEYLKSTGI
CCCCEEECCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDTAYFGPEPEFFVFDEVKWSVGMSGVSHEIVAEEAAWSTNKDYEWGNMGHRPRVKGGYV
CCCCCCCCCCCEEEEECEEHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PVPPVDSAQDMRTVMCDRIEDMIGEGCIEVHHHEVAPCQLEIGVAFNTLVRKADEVQKLK
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHH
YAVHNVAHQFGKTATFMPKPIVGDNGSGMHVHISISKDGVNTFSGDEYAGLSETALYFVG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
GIIKHARALNAITNPSTNSYKRLVPHYEAPIKLAYSASNRSASIRIPHVSSPKAVRVEAR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEEEEE
FPDPAANPYLAFAALLMAGIDGIQNKMHPGEAADKNLYDLPPEEEALIPTVAENLEVALQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALKDDHEFLLKGDVFTKEMLEAYIALKQEEAQRVNITVHPVEFDLYYSC
HHCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECC
>Mature Secondary Structure 
SNKLLDLIKSSNAKWVDFRFTDTRGKEHHMSFPAHSVDEESMEDGKMFDGSSIAGWKGI
CCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCC
EASDMILRPDPDTAFIDPFFDAVTVVVTCDIIEPSTLQGYDRDPRSIARRAEEYLKSTGI
CCCCEEECCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDTAYFGPEPEFFVFDEVKWSVGMSGVSHEIVAEEAAWSTNKDYEWGNMGHRPRVKGGYV
CCCCCCCCCCCEEEEECEEHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PVPPVDSAQDMRTVMCDRIEDMIGEGCIEVHHHEVAPCQLEIGVAFNTLVRKADEVQKLK
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHH
YAVHNVAHQFGKTATFMPKPIVGDNGSGMHVHISISKDGVNTFSGDEYAGLSETALYFVG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
GIIKHARALNAITNPSTNSYKRLVPHYEAPIKLAYSASNRSASIRIPHVSSPKAVRVEAR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEEEEE
FPDPAANPYLAFAALLMAGIDGIQNKMHPGEAADKNLYDLPPEEEALIPTVAENLEVALQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALKDDHEFLLKGDVFTKEMLEAYIALKQEEAQRVNITVHPVEFDLYYSC
HHCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]