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The map label for this gene is aspA [H]

Identifier: 93006243

GI number: 93006243

Start: 1739051

End: 1740463

Strand: Direct

Name: aspA [H]

Synonym: Pcryo_1417

Alternate gene names: 93006243

Gene position: 1739051-1740463 (Clockwise)

Preceding gene: 93006242

Following gene: 93006248

Centisome position: 56.83

GC content: 44.66

Gene sequence:

>1413_bases
ATGACAACCACTACTCGCCAAGAATACGACTTACTTGGTCATCGCGATGTGCCAGCTGACGCTTACTACGGCATACAAAC
CTTACGTGCTTTTGAAAACTTCACTGTGAGCGGTGTGCGCTTACATCAATTCTCTACCTTTATCCGTGCCTTTGCTACCG
TCAAAAAAGCCGCTGCCATTGCCAATAACAAAGTTGGTGTATTAAATGATACCGTCAAAGATGCGATCGTCGCGGCATGT
GATGACTTAATCGCTGATAAATATCATGACCAATTTATCGTCGATATGTTTCAAGGCGGCGCAGGCACCTCAACCAATAT
GAATGTCAATGAGGTCGTTGCCAATCGTGCTTTAGAGATATTAGGCTATGAAAAAGGGCAATATCAGCATGTGCATCCAA
ATAATCATGTCAACCTTTCGCAGTCGACCAATGATGCCTATCCAACGGCGCTTAATGTCGCATTGGATGACTATTGCACG
CGTCTATTGGATAAGATGGCGGTGCTGCACCAAAGTCTAATTAACAAAAGCATCGAATTCGCCGATCAATTAAAAATGGG
ACGCACCCATCTACAAGATGCGGTGCCGATGACAGCAGGTCAAGAATTCAACGCTTTTGCAACGATGGTCAAAAAAGAGA
TGGCGCGTATCGAGTCAACTCGCGCACTTTTTCATGAGATTAATATGGGCGGTACCGCTATCGGTACTGGCTTGAATGCA
CCTGCTGGGTATTCAGAGCTGTGTGCTGAGACATTACAAGAGCTAACTGGCAGACCTTATTATATCGCTGAAGATTTGAT
TGAAGCCACACAAGATACCAGTGCTTATGTTGCTTTGTCTGGCAATCTGCGTCTATTTGCTGCTCGTCTATCAAAGATTG
CTCATGACTTACGCTTGCAATCCTCGGGTCCTCGTGCAGGCTTGGCACAATATCGCCTGCCTGAGATGCAGCCTGGTAGC
TCAATCATGCCGGGTAAAGTAAACCCTGTTATACCTGAAGTGGTCAATCAAGCGTGTTTTCATGTAATGGGTATTGATAC
CGCTATCGTCATGGCATCAGAGAACGCTCAGCTACAACTCAATGTCTTTGAGCCAGTCATTGCTTTCAACTTATTTACCG
GTATGAGCATGATGGCAAATATCTGTGAGATATTTGCCACGCGCTGTATCGATGGCGTAATGATGAATAATGAAGCGTGT
AATACTGAAGTATTCAACAATATTGGTCTGGTAACCGCATTAAACCCACATTTGGGTTATGAGTCGTCTACCTATGTGGC
AAGAAAAGCCCAAGAAACCGGTGGTCGTGTTTATGACATCGTTCTAGAAGAGAACCTGATGGATAAAGAGACGTTAGACA
ATCTACTCTCTCCTGCTAACATGGTATATCCAAAGGTCAAACTAACCCCTTAG

Upstream 100 bases:

>100_bases
CTCATTTGAGGCGACATCGCCATAGTGATAACAGGGTTTTATAAATATCGCAGGCGATGGCCGTTTTTATCAGGGCTTTT
TATTGAAGGAATTTTATTAA

Downstream 100 bases:

>100_bases
TTTTTCTTTTTTAACGTAAAAAGGCTGGGTTAGCAATTGCTAATCCAGCCTTTTTTTATTTTATTCATACCTGCATTATT
TGGGGAAAGCCTATAAGCTA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTTTTRQEYDLLGHRDVPADAYYGIQTLRAFENFTVSGVRLHQFSTFIRAFATVKKAAAIANNKVGVLNDTVKDAIVAAC
DDLIADKYHDQFIVDMFQGGAGTSTNMNVNEVVANRALEILGYEKGQYQHVHPNNHVNLSQSTNDAYPTALNVALDDYCT
RLLDKMAVLHQSLINKSIEFADQLKMGRTHLQDAVPMTAGQEFNAFATMVKKEMARIESTRALFHEINMGGTAIGTGLNA
PAGYSELCAETLQELTGRPYYIAEDLIEATQDTSAYVALSGNLRLFAARLSKIAHDLRLQSSGPRAGLAQYRLPEMQPGS
SIMPGKVNPVIPEVVNQACFHVMGIDTAIVMASENAQLQLNVFEPVIAFNLFTGMSMMANICEIFATRCIDGVMMNNEAC
NTEVFNNIGLVTALNPHLGYESSTYVARKAQETGGRVYDIVLEENLMDKETLDNLLSPANMVYPKVKLTP

Sequences:

>Translated_470_residues
MTTTTRQEYDLLGHRDVPADAYYGIQTLRAFENFTVSGVRLHQFSTFIRAFATVKKAAAIANNKVGVLNDTVKDAIVAAC
DDLIADKYHDQFIVDMFQGGAGTSTNMNVNEVVANRALEILGYEKGQYQHVHPNNHVNLSQSTNDAYPTALNVALDDYCT
RLLDKMAVLHQSLINKSIEFADQLKMGRTHLQDAVPMTAGQEFNAFATMVKKEMARIESTRALFHEINMGGTAIGTGLNA
PAGYSELCAETLQELTGRPYYIAEDLIEATQDTSAYVALSGNLRLFAARLSKIAHDLRLQSSGPRAGLAQYRLPEMQPGS
SIMPGKVNPVIPEVVNQACFHVMGIDTAIVMASENAQLQLNVFEPVIAFNLFTGMSMMANICEIFATRCIDGVMMNNEAC
NTEVFNNIGLVTALNPHLGYESSTYVARKAQETGGRVYDIVLEENLMDKETLDNLLSPANMVYPKVKLTP
>Mature_469_residues
TTTTRQEYDLLGHRDVPADAYYGIQTLRAFENFTVSGVRLHQFSTFIRAFATVKKAAAIANNKVGVLNDTVKDAIVAACD
DLIADKYHDQFIVDMFQGGAGTSTNMNVNEVVANRALEILGYEKGQYQHVHPNNHVNLSQSTNDAYPTALNVALDDYCTR
LLDKMAVLHQSLINKSIEFADQLKMGRTHLQDAVPMTAGQEFNAFATMVKKEMARIESTRALFHEINMGGTAIGTGLNAP
AGYSELCAETLQELTGRPYYIAEDLIEATQDTSAYVALSGNLRLFAARLSKIAHDLRLQSSGPRAGLAQYRLPEMQPGSS
IMPGKVNPVIPEVVNQACFHVMGIDTAIVMASENAQLQLNVFEPVIAFNLFTGMSMMANICEIFATRCIDGVMMNNEACN
TEVFNNIGLVTALNPHLGYESSTYVARKAQETGGRVYDIVLEENLMDKETLDNLLSPANMVYPKVKLTP

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=41.648590021692, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI87082375, Length=468, Percent_Identity=51.2820512820513, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787896, Length=460, Percent_Identity=40.2173913043478, Blast_Score=344, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=41.648590021692, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=39.6341463414634, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324993, Length=466, Percent_Identity=39.9141630901288, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=40.3887688984881, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=39.8268398268398, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24640179, Length=462, Percent_Identity=39.8268398268398, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=39.3088552915767, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24583245, Length=467, Percent_Identity=37.2591006423983, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51615; Mature: 51484

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTRQEYDLLGHRDVPADAYYGIQTLRAFENFTVSGVRLHQFSTFIRAFATVKKAAAI
CCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ANNKVGVLNDTVKDAIVAACDDLIADKYHDQFIVDMFQGGAGTSTNMNVNEVVANRALEI
HCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGYEKGQYQHVHPNNHVNLSQSTNDAYPTALNVALDDYCTRLLDKMAVLHQSLINKSIEF
HCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADQLKMGRTHLQDAVPMTAGQEFNAFATMVKKEMARIESTRALFHEINMGGTAIGTGLNA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PAGYSELCAETLQELTGRPYYIAEDLIEATQDTSAYVALSGNLRLFAARLSKIAHDLRLQ
CCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCC
SSGPRAGLAQYRLPEMQPGSSIMPGKVNPVIPEVVNQACFHVMGIDTAIVMASENAQLQL
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
NVFEPVIAFNLFTGMSMMANICEIFATRCIDGVMMNNEACNTEVFNNIGLVTALNPHLGY
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHCCCEEEEECCCCCC
ESSTYVARKAQETGGRVYDIVLEENLMDKETLDNLLSPANMVYPKVKLTP
CCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCHHHCCEEEECC
>Mature Secondary Structure 
TTTTRQEYDLLGHRDVPADAYYGIQTLRAFENFTVSGVRLHQFSTFIRAFATVKKAAAI
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ANNKVGVLNDTVKDAIVAACDDLIADKYHDQFIVDMFQGGAGTSTNMNVNEVVANRALEI
HCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGYEKGQYQHVHPNNHVNLSQSTNDAYPTALNVALDDYCTRLLDKMAVLHQSLINKSIEF
HCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADQLKMGRTHLQDAVPMTAGQEFNAFATMVKKEMARIESTRALFHEINMGGTAIGTGLNA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PAGYSELCAETLQELTGRPYYIAEDLIEATQDTSAYVALSGNLRLFAARLSKIAHDLRLQ
CCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCC
SSGPRAGLAQYRLPEMQPGSSIMPGKVNPVIPEVVNQACFHVMGIDTAIVMASENAQLQL
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
NVFEPVIAFNLFTGMSMMANICEIFATRCIDGVMMNNEACNTEVFNNIGLVTALNPHLGY
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHCCCEEEEECCCCCC
ESSTYVARKAQETGGRVYDIVLEENLMDKETLDNLLSPANMVYPKVKLTP
CCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]