Click here to switch to the map view.

The map label for this gene is benM [H]

Identifier: 93006141

GI number: 93006141

Start: 1604212

End: 1605237

Strand: Reverse

Name: benM [H]

Synonym: Pcryo_1313

Alternate gene names: 93006141

Gene position: 1605237-1604212 (Counterclockwise)

Preceding gene: 93006143

Following gene: 93006140

Centisome position: 52.46

GC content: 46.59

Gene sequence:

>1026_bases
ATGGATTTAAAGCAGCTCAATGCGTTTATTGCCGTTGCCGAGCTTCGGAGCTTTAGTGCAGCCGCTACCAAAATCGGACT
GTCACAGCCCAGTTTAAGCCGTTTACTGAAGCAACTTGAGATAGACATGGGCGTGATGTTGGTTGATCGTTATCATAGAC
CGCTACATTTAACAGAAGCTGGCACGTTCTTTTACGATAAAATCAGTACCATACTGACAGAAGTCGATACGGTTGTTGGT
ATGACCCAGCGCTTATCTGCCCCTAGCAGCGCATTAAATATCGGTTTTGTACCCTCTATACTGTATGGGTTGCTACCTGA
GATTATTGCCAGACTGAAGCAGTCCAATCCTGAGATTGACGTCAACCTAAAGGACATTAGCTCCTATCAGCAAATGGATG
CGCTCAAATCTGGTGATATCGATATTGGTTTTGGACGCTTTGCCCATCAAGACCCATGGATACAGCAAATACTGTTACGC
CACGAGCGCTATGTCGTTGCCCTCCCCAAAGCCCATCCGCTTGCCAATGTGCGCGAGCAACGCTTGATCGATTTGGCCAA
TAACCGCCTGATTCTCTATCATCAAACCCACTTGCCTGTATCTACAATGCCAGAAACGGTATCAAGCACAGGGACTCCTA
AAACCTTGGGGCAGAATAAGAAAAATAATACGAGCCATCATCAAGCCTCTACTGTTGAGCAGATAACAGAGCCTTTACTA
TATTTATTTGCACAGTACGGCATCTCTCCATTTATGACCACCACCGTTAGTGACTTGCAAGTGGCACTCGGGTTAGTAGC
GGCGGGCGAAGGCATCACCTTAGTCCCTGCCAGCTTAAAAACGGTGCGTACGGAGCAGATCAGCTATCAGCGTCTCATTC
ACGAAAACGTCACCTCACCTATTTATCTGCATACACTTAAGGATTTTGTGCATCCTCATATTCCTGACTTATTGCGTGCG
ACTTATCATGTCTATGAGCAGCGCGGCATTACTTACCGCCGGCAAAAATTTGAATCTCAGCGCTAA

Upstream 100 bases:

>100_bases
GCTTCAAGCATAATTTTATAGTTTTGCTATTATTGATATAAAATACAGTAGTATCCGCTTCATCAATCGACATGATATTA
CAGATAAATGAGCATAAAAC

Downstream 100 bases:

>100_bases
ATTATCAGATATTGAGTCATAATTGTGGTATAAAGAAGCCCGAAAAAATAGCCATTGATAAATCTTGATATTTACATTTA
ATTATGCTAACTATCAGGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MDLKQLNAFIAVAELRSFSAAATKIGLSQPSLSRLLKQLEIDMGVMLVDRYHRPLHLTEAGTFFYDKISTILTEVDTVVG
MTQRLSAPSSALNIGFVPSILYGLLPEIIARLKQSNPEIDVNLKDISSYQQMDALKSGDIDIGFGRFAHQDPWIQQILLR
HERYVVALPKAHPLANVREQRLIDLANNRLILYHQTHLPVSTMPETVSSTGTPKTLGQNKKNNTSHHQASTVEQITEPLL
YLFAQYGISPFMTTTVSDLQVALGLVAAGEGITLVPASLKTVRTEQISYQRLIHENVTSPIYLHTLKDFVHPHIPDLLRA
TYHVYEQRGITYRRQKFESQR

Sequences:

>Translated_341_residues
MDLKQLNAFIAVAELRSFSAAATKIGLSQPSLSRLLKQLEIDMGVMLVDRYHRPLHLTEAGTFFYDKISTILTEVDTVVG
MTQRLSAPSSALNIGFVPSILYGLLPEIIARLKQSNPEIDVNLKDISSYQQMDALKSGDIDIGFGRFAHQDPWIQQILLR
HERYVVALPKAHPLANVREQRLIDLANNRLILYHQTHLPVSTMPETVSSTGTPKTLGQNKKNNTSHHQASTVEQITEPLL
YLFAQYGISPFMTTTVSDLQVALGLVAAGEGITLVPASLKTVRTEQISYQRLIHENVTSPIYLHTLKDFVHPHIPDLLRA
TYHVYEQRGITYRRQKFESQR
>Mature_341_residues
MDLKQLNAFIAVAELRSFSAAATKIGLSQPSLSRLLKQLEIDMGVMLVDRYHRPLHLTEAGTFFYDKISTILTEVDTVVG
MTQRLSAPSSALNIGFVPSILYGLLPEIIARLKQSNPEIDVNLKDISSYQQMDALKSGDIDIGFGRFAHQDPWIQQILLR
HERYVVALPKAHPLANVREQRLIDLANNRLILYHQTHLPVSTMPETVSSTGTPKTLGQNKKNNTSHHQASTVEQITEPLL
YLFAQYGISPFMTTTVSDLQVALGLVAAGEGITLVPASLKTVRTEQISYQRLIHENVTSPIYLHTLKDFVHPHIPDLLRA
TYHVYEQRGITYRRQKFESQR

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=308, Percent_Identity=29.2207792207792, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787879, Length=305, Percent_Identity=25.5737704918033, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=27.6978417266187, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI2367136, Length=321, Percent_Identity=25.2336448598131, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787601, Length=189, Percent_Identity=26.984126984127, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI157672245, Length=196, Percent_Identity=29.0816326530612, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081904, Length=190, Percent_Identity=29.4736842105263, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=143, Percent_Identity=34.2657342657343, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=206, Percent_Identity=25.2427184466019, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=171, Percent_Identity=25.7309941520468, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786401, Length=122, Percent_Identity=31.9672131147541, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=174, Percent_Identity=30.4597701149425, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789639, Length=125, Percent_Identity=32.8, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38389; Mature: 38389

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKQLNAFIAVAELRSFSAAATKIGLSQPSLSRLLKQLEIDMGVMLVDRYHRPLHLTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
GTFFYDKISTILTEVDTVVGMTQRLSAPSSALNIGFVPSILYGLLPEIIARLKQSNPEID
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHCCCCCEE
VNLKDISSYQQMDALKSGDIDIGFGRFAHQDPWIQQILLRHERYVVALPKAHPLANVREQ
EEHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHH
RLIDLANNRLILYHQTHLPVSTMPETVSSTGTPKTLGQNKKNNTSHHQASTVEQITEPLL
HHHHHCCCEEEEEEECCCCHHHCCHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
YLFAQYGISPFMTTTVSDLQVALGLVAAGEGITLVPASLKTVRTEQISYQRLIHENVTSP
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCC
IYLHTLKDFVHPHIPDLLRATYHVYEQRGITYRRQKFESQR
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCC
>Mature Secondary Structure
MDLKQLNAFIAVAELRSFSAAATKIGLSQPSLSRLLKQLEIDMGVMLVDRYHRPLHLTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
GTFFYDKISTILTEVDTVVGMTQRLSAPSSALNIGFVPSILYGLLPEIIARLKQSNPEID
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHHHHHHHCCCCCEE
VNLKDISSYQQMDALKSGDIDIGFGRFAHQDPWIQQILLRHERYVVALPKAHPLANVREQ
EEHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHH
RLIDLANNRLILYHQTHLPVSTMPETVSSTGTPKTLGQNKKNNTSHHQASTVEQITEPLL
HHHHHCCCEEEEEEECCCCHHHCCHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
YLFAQYGISPFMTTTVSDLQVALGLVAAGEGITLVPASLKTVRTEQISYQRLIHENVTSP
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCC
IYLHTLKDFVHPHIPDLLRATYHVYEQRGITYRRQKFESQR
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]