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The map label for this gene is benM [H]

Identifier: 93006099

GI number: 93006099

Start: 1541957

End: 1542874

Strand: Direct

Name: benM [H]

Synonym: Pcryo_1271

Alternate gene names: 93006099

Gene position: 1541957-1542874 (Clockwise)

Preceding gene: 93006084

Following gene: 93006101

Centisome position: 50.39

GC content: 38.89

Gene sequence:

>918_bases
ATGGAATTGAGACATTTACGCTACTTTGTAGCAGTGGCAGAGGAAAAAAGTTTTAATAAGGCAGCAGAGCGTTTGTTTAT
TTCACAACCACCACTGAGCCGTCAGATAAAACAATTAGAAGAAGAGATAGGCGTCACGCTCATTGATAGGGACAATCGCC
CTTTAAAACTTACTGAAGCTGGCGAGTTTTTTTATGATCATGCGCTGCAGATAATAAAGAAATCAGATGATCTGCGTGCT
ATGACTTTTCGTAAGGGTAATTTTGACAACTCACTCTCTATAGGTTTTGTATCGTCGATATTATATGGCATATTGCCAAA
AGTGATTGCGCGCTTCAGAGAGGTCTATCCAAATATAGAAATTAAGTTATATGAATTAAACTCTTGGCAGCAAACACAAA
GCTTGGTAGAGGGTAAAATAGATGTTGGTTATGGAAGGTTATTATTCGAAGACGCTTCTATTAGACGATTACTATTAAGA
GAGGAGAGCTTAGTTGTAGCTGTTCCTGTTAAACATCGTTTGGCACAAGAGAAGCAAAAGAGCATTCGTTTAATGGACTT
AACCAATGAAAACTTATTGTTATATCCGATGGCACCTCGTCCTAGTTTTATTGACTATGTCTTAGAGTTGTTTGAGGCAC
GCAATCTAAAAGCAAACTATTTTACTGAGGTACGTGAGCTGCATATTGCGTTAGGGTTGGTAGCAGCAGGTGAGGGCTTG
ACTATCGTACCAAAAACGCTTGAGCATCTGCGGAGTCAGGAAGTGACTTATATTCCTTTCGAGGACGGGCTTTTAACCTC
TCCTATTGTCATGAGTGTGAGACACTTTGACAAGTCAGAATTGTTAAAAACATTGTTAGACGTGACTTATCAGATTTATG
ATGAAGAAGGGTTTGATTATAAGCGTGAGACAATATGA

Upstream 100 bases:

>100_bases
CTATTATTAATACCTTTTAGGTATTTTGATAACCAACCCGCACCATGTTATGTTCTTTAGGTATCGATAAGCTTATAGAT
CACTCAAGGAATAACGTTCA

Downstream 100 bases:

>100_bases
ATACATCATAGTGTTAATATAGAATATAAACCTATGCGTGAGAATTTACGAGTGCTAGTTCAATAAACGAGTCATTGCCG
TTAAGCCCAATAAAAGCTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MELRHLRYFVAVAEEKSFNKAAERLFISQPPLSRQIKQLEEEIGVTLIDRDNRPLKLTEAGEFFYDHALQIIKKSDDLRA
MTFRKGNFDNSLSIGFVSSILYGILPKVIARFREVYPNIEIKLYELNSWQQTQSLVEGKIDVGYGRLLFEDASIRRLLLR
EESLVVAVPVKHRLAQEKQKSIRLMDLTNENLLLYPMAPRPSFIDYVLELFEARNLKANYFTEVRELHIALGLVAAGEGL
TIVPKTLEHLRSQEVTYIPFEDGLLTSPIVMSVRHFDKSELLKTLLDVTYQIYDEEGFDYKRETI

Sequences:

>Translated_305_residues
MELRHLRYFVAVAEEKSFNKAAERLFISQPPLSRQIKQLEEEIGVTLIDRDNRPLKLTEAGEFFYDHALQIIKKSDDLRA
MTFRKGNFDNSLSIGFVSSILYGILPKVIARFREVYPNIEIKLYELNSWQQTQSLVEGKIDVGYGRLLFEDASIRRLLLR
EESLVVAVPVKHRLAQEKQKSIRLMDLTNENLLLYPMAPRPSFIDYVLELFEARNLKANYFTEVRELHIALGLVAAGEGL
TIVPKTLEHLRSQEVTYIPFEDGLLTSPIVMSVRHFDKSELLKTLLDVTYQIYDEEGFDYKRETI
>Mature_305_residues
MELRHLRYFVAVAEEKSFNKAAERLFISQPPLSRQIKQLEEEIGVTLIDRDNRPLKLTEAGEFFYDHALQIIKKSDDLRA
MTFRKGNFDNSLSIGFVSSILYGILPKVIARFREVYPNIEIKLYELNSWQQTQSLVEGKIDVGYGRLLFEDASIRRLLLR
EESLVVAVPVKHRLAQEKQKSIRLMDLTNENLLLYPMAPRPSFIDYVLELFEARNLKANYFTEVRELHIALGLVAAGEGL
TIVPKTLEHLRSQEVTYIPFEDGLLTSPIVMSVRHFDKSELLKTLLDVTYQIYDEEGFDYKRETI

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=35.191637630662, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787879, Length=283, Percent_Identity=34.2756183745583, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=30.0813008130081, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693105, Length=267, Percent_Identity=30.3370786516854, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=28.1368821292776, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI157672245, Length=192, Percent_Identity=34.375, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081904, Length=189, Percent_Identity=31.2169312169312, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=32.4137931034483, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=27.734375, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787806, Length=248, Percent_Identity=26.2096774193548, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788748, Length=251, Percent_Identity=28.2868525896414, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788297, Length=262, Percent_Identity=27.8625954198473, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082132, Length=170, Percent_Identity=30.5882352941176, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=144, Percent_Identity=32.6388888888889, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=166, Percent_Identity=31.3253012048193, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787530, Length=203, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789173, Length=147, Percent_Identity=31.9727891156463, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=300, Percent_Identity=23.6666666666667, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789204, Length=250, Percent_Identity=25.6, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35388; Mature: 35388

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFVAVAEEKSFNKAAERLFISQPPLSRQIKQLEEEIGVTLIDRDNRPLKLTEA
CCHHHHEEEEHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEECC
GEFFYDHALQIIKKSDDLRAMTFRKGNFDNSLSIGFVSSILYGILPKVIARFREVYPNIE
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IKLYELNSWQQTQSLVEGKIDVGYGRLLFEDASIRRLLLREESLVVAVPVKHRLAQEKQK
EEEEECCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEECHHHHHHHHHHH
SIRLMDLTNENLLLYPMAPRPSFIDYVLELFEARNLKANYFTEVRELHIALGLVAAGEGL
CEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCC
TIVPKTLEHLRSQEVTYIPFEDGLLTSPIVMSVRHFDKSELLKTLLDVTYQIYDEEGFDY
EECHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCC
KRETI
CCCCC
>Mature Secondary Structure
MELRHLRYFVAVAEEKSFNKAAERLFISQPPLSRQIKQLEEEIGVTLIDRDNRPLKLTEA
CCHHHHEEEEHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEECC
GEFFYDHALQIIKKSDDLRAMTFRKGNFDNSLSIGFVSSILYGILPKVIARFREVYPNIE
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IKLYELNSWQQTQSLVEGKIDVGYGRLLFEDASIRRLLLREESLVVAVPVKHRLAQEKQK
EEEEECCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEECHHHHHHHHHHH
SIRLMDLTNENLLLYPMAPRPSFIDYVLELFEARNLKANYFTEVRELHIALGLVAAGEGL
CEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCC
TIVPKTLEHLRSQEVTYIPFEDGLLTSPIVMSVRHFDKSELLKTLLDVTYQIYDEEGFDY
EECHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCC
KRETI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]