Click here to switch to the map view.

The map label for this gene is trmJ [H]

Identifier: 93006040

GI number: 93006040

Start: 1466868

End: 1467749

Strand: Reverse

Name: trmJ [H]

Synonym: Pcryo_1212

Alternate gene names: 93006040

Gene position: 1467749-1466868 (Counterclockwise)

Preceding gene: 93006041

Following gene: 93006039

Centisome position: 47.97

GC content: 45.01

Gene sequence:

>882_bases
ATGAGTAACAATGCACCCTCATCTTTAGCAACAGCAGTATCAAACGTCAGCAATTATTTGTCTTGTGTTCAAGTAGTGAT
GGTCAATACGACATTGCCTGCCAATATTGGTTCAGCAGCACGCGCCATGCACACCATGGGGTTATCGAGATTGACGGTGG
TTGATCCAAAATTACCGATTGATGACACTAGCGTCTCACATGCTGCTGGTGGCAGTGATTTGCTATCATCAGCAATCATC
GCACCAACGCTTGAGTCTGCTATCGCTGATTGCCAATTGGTATTTGCGGCCAGTAGTCGTAGCCGTCATTTGCCGCGTCC
TGTGGTCACGCCGACACAAGCTGCCAAAATCATGTTGGACTTTATTGATAAGCAAAGCACAGTTAATGGGCAAGATGGTA
TAAATACAGCGCCCAATATTGCTATCCTGTTTGGGCGTGAAGATCGCGGCTTGACCAATGAAGAATTGGCTTATGCTGAC
TATCATGTTCAAATTGATGCCAACCCTGCTTATCCAGTGCTCAATGTTGCTTCAGCCGTGCAAGTGATCGCGAGCTTTAT
TTATGCTTATGCACAAACTCATATCCAAGCTGACAGCGAAGTTAATGATATAGCACAGGTAACTGACAACCTAGAGACGC
TTGAACAAGGGATGCTTAATACCTATGTGCGCCAGCAATGGGATGAGCCAGCGATTACTCAACAGCAATTGCAACTGCTT
ACTGAGCGTACCACCGCATTAATGGTTCAACGCAAACTGGCAGATAGTGAAAACTTAAAATCGCTGCCATCACGCTTATC
TAGACTCGGTTCACGTATTCAACTCGACCAAAAAGAGTATCAGTTACTCAGTGCTCTGCTAGCAAAACTTTTAAAAGACT
AA

Upstream 100 bases:

>100_bases
GCCGTGAGCGTCTTATCAGTGAACAAGCCCCTCTTATCGAGGACATCGCAGTTGCATTGCAACGTGCTGAGCAAGACATC
AACCAGCGACTAGGACAATA

Downstream 100 bases:

>100_bases
CATTTAATCAGTTAATTTTCTTGCCACTATGGGCATTATTAGTAGTACAGGTAGTCATATCATTTAACAATAAAAAGGGA
TTTACGATGAATTGGACTGT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSNNAPSSLATAVSNVSNYLSCVQVVMVNTTLPANIGSAARAMHTMGLSRLTVVDPKLPIDDTSVSHAAGGSDLLSSAII
APTLESAIADCQLVFAASSRSRHLPRPVVTPTQAAKIMLDFIDKQSTVNGQDGINTAPNIAILFGREDRGLTNEELAYAD
YHVQIDANPAYPVLNVASAVQVIASFIYAYAQTHIQADSEVNDIAQVTDNLETLEQGMLNTYVRQQWDEPAITQQQLQLL
TERTTALMVQRKLADSENLKSLPSRLSRLGSRIQLDQKEYQLLSALLAKLLKD

Sequences:

>Translated_293_residues
MSNNAPSSLATAVSNVSNYLSCVQVVMVNTTLPANIGSAARAMHTMGLSRLTVVDPKLPIDDTSVSHAAGGSDLLSSAII
APTLESAIADCQLVFAASSRSRHLPRPVVTPTQAAKIMLDFIDKQSTVNGQDGINTAPNIAILFGREDRGLTNEELAYAD
YHVQIDANPAYPVLNVASAVQVIASFIYAYAQTHIQADSEVNDIAQVTDNLETLEQGMLNTYVRQQWDEPAITQQQLQLL
TERTTALMVQRKLADSENLKSLPSRLSRLGSRIQLDQKEYQLLSALLAKLLKD
>Mature_292_residues
SNNAPSSLATAVSNVSNYLSCVQVVMVNTTLPANIGSAARAMHTMGLSRLTVVDPKLPIDDTSVSHAAGGSDLLSSAIIA
PTLESAIADCQLVFAASSRSRHLPRPVVTPTQAAKIMLDFIDKQSTVNGQDGINTAPNIAILFGREDRGLTNEELAYADY
HVQIDANPAYPVLNVASAVQVIASFIYAYAQTHIQADSEVNDIAQVTDNLETLEQGMLNTYVRQQWDEPAITQQQLQLLT
ERTTALMVQRKLADSENLKSLPSRLSRLGSRIQLDQKEYQLLSALLAKLLKD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=235, Percent_Identity=37.8723404255319, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790865, Length=271, Percent_Identity=29.520295202952, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31732; Mature: 31601

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNAPSSLATAVSNVSNYLSCVQVVMVNTTLPANIGSAARAMHTMGLSRLTVVDPKLPI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
DDTSVSHAAGGSDLLSSAIIAPTLESAIADCQLVFAASSRSRHLPRPVVTPTQAAKIMLD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHH
FIDKQSTVNGQDGINTAPNIAILFGREDRGLTNEELAYADYHVQIDANPAYPVLNVASAV
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHH
QVIASFIYAYAQTHIQADSEVNDIAQVTDNLETLEQGMLNTYVRQQWDEPAITQQQLQLL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TERTTALMVQRKLADSENLKSLPSRLSRLGSRIQLDQKEYQLLSALLAKLLKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNNAPSSLATAVSNVSNYLSCVQVVMVNTTLPANIGSAARAMHTMGLSRLTVVDPKLPI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
DDTSVSHAAGGSDLLSSAIIAPTLESAIADCQLVFAASSRSRHLPRPVVTPTQAAKIMLD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHH
FIDKQSTVNGQDGINTAPNIAILFGREDRGLTNEELAYADYHVQIDANPAYPVLNVASAV
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHH
QVIASFIYAYAQTHIQADSEVNDIAQVTDNLETLEQGMLNTYVRQQWDEPAITQQQLQLL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TERTTALMVQRKLADSENLKSLPSRLSRLGSRIQLDQKEYQLLSALLAKLLKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA