Click here to switch to the map view.

The map label for this gene is htrB [H]

Identifier: 93006010

GI number: 93006010

Start: 1429990

End: 1430916

Strand: Reverse

Name: htrB [H]

Synonym: Pcryo_1182

Alternate gene names: 93006010

Gene position: 1430916-1429990 (Counterclockwise)

Preceding gene: 93006011

Following gene: 93006009

Centisome position: 46.76

GC content: 43.26

Gene sequence:

>927_bases
ATGAATCCGTATCATATTTTCAAAATCGTGCCACTGTCTGTCTTGCAGATGCTGGCACGTTTTGTGGCGTGGATAATCTT
AAGGATGCCAAGCGCTAGTATCATGCGCACAGTTAAAATCAATATGGCACTGATTGCCCCTACGTTATCTCACCTGCAAA
AACAAGCCTTAACCAAAGAGATTATCTATCATCAATGTTTAACCAGCATTGAGTCAATCAAAAGCTGGGCGATGCCACCT
GATTGGAGTATCGCTCAAATTCGTGACGTTCATAATAAAGACATCTTACTTGATGGACTTGCTAGTCCTAACGGCATGCT
CGCGATCGTACCGCATTTGGGTACGTGGGAGATGATGAATGCTTGGCTCAATCAATTTGGCTCGCCAACCATCATGTATA
AGCCCGTAAAAGGTGACATTACCAACAATTTTATATTGCAAGGTCGTGCCCGCCTCAATGCGACATTAGTACCGACGGAT
GGTAGCGGTGTAAAAGCAGTATTTAAAACACTCAAACAAGGTGGTTTTAGTATTGTCTTACCTGACCACGTTCCTGACCC
ATCAGGCGGTGTCATCGTGCCTTTTTTTGGCATTGAAACGTTGACCAGTACACTCGCCTCCAAACTGGCTAGTAAAACCA
AATGCGCACTGGTCGGATTGGCATGTATTCGCCGCGACGATGGGCATGGCTTTGATATTTATTGCTATAAACTCGATGAC
CCAGCATTATATGATCGCAATACTGAAACGGCAGCTTATGCACTCAATCTAGCAATGCAACGTATGATTGAAGACAATTA
CAGTCATTATATGTGGGGTTATCGACGCTTTAAGCATATCCCTATGATAGACAACCCTTACAACACTAATAAAGCCAATC
TAGCCGCATTTATTCGCGCACATCATCCTAGTGTCGATAATAAATAA

Upstream 100 bases:

>100_bases
GTACAAGCTGACACCAGTAAGTCTGCTGAATAAATAGTCTGATCATGCGAAGCTATTTTTTACAGTGCCTGTTATGTGAT
TCATTACTGTGTGAATGGTC

Downstream 100 bases:

>100_bases
AATCGATACAGTGAAATCCGTACGCTACTGATAGACTCTCAATTTTAGTGTTGTTTATCAATTTTTTAAAAGTTAGCCCC
TTATTTATATAAATATGAGT

Product: lipid A biosynthesis acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNPYHIFKIVPLSVLQMLARFVAWIILRMPSASIMRTVKINMALIAPTLSHLQKQALTKEIIYHQCLTSIESIKSWAMPP
DWSIAQIRDVHNKDILLDGLASPNGMLAIVPHLGTWEMMNAWLNQFGSPTIMYKPVKGDITNNFILQGRARLNATLVPTD
GSGVKAVFKTLKQGGFSIVLPDHVPDPSGGVIVPFFGIETLTSTLASKLASKTKCALVGLACIRRDDGHGFDIYCYKLDD
PALYDRNTETAAYALNLAMQRMIEDNYSHYMWGYRRFKHIPMIDNPYNTNKANLAAFIRAHHPSVDNK

Sequences:

>Translated_308_residues
MNPYHIFKIVPLSVLQMLARFVAWIILRMPSASIMRTVKINMALIAPTLSHLQKQALTKEIIYHQCLTSIESIKSWAMPP
DWSIAQIRDVHNKDILLDGLASPNGMLAIVPHLGTWEMMNAWLNQFGSPTIMYKPVKGDITNNFILQGRARLNATLVPTD
GSGVKAVFKTLKQGGFSIVLPDHVPDPSGGVIVPFFGIETLTSTLASKLASKTKCALVGLACIRRDDGHGFDIYCYKLDD
PALYDRNTETAAYALNLAMQRMIEDNYSHYMWGYRRFKHIPMIDNPYNTNKANLAAFIRAHHPSVDNK
>Mature_308_residues
MNPYHIFKIVPLSVLQMLARFVAWIILRMPSASIMRTVKINMALIAPTLSHLQKQALTKEIIYHQCLTSIESIKSWAMPP
DWSIAQIRDVHNKDILLDGLASPNGMLAIVPHLGTWEMMNAWLNQFGSPTIMYKPVKGDITNNFILQGRARLNATLVPTD
GSGVKAVFKTLKQGGFSIVLPDHVPDPSGGVIVPFFGIETLTSTLASKLASKTKCALVGLACIRRDDGHGFDIYCYKLDD
PALYDRNTETAAYALNLAMQRMIEDNYSHYMWGYRRFKHIPMIDNPYNTNKANLAAFIRAHHPSVDNK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=294, Percent_Identity=26.530612244898, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 34526; Mature: 34526

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPYHIFKIVPLSVLQMLARFVAWIILRMPSASIMRTVKINMALIAPTLSHLQKQALTKE
CCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHHH
IIYHQCLTSIESIKSWAMPPDWSIAQIRDVHNKDILLDGLASPNGMLAIVPHLGTWEMMN
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCHHHHH
AWLNQFGSPTIMYKPVKGDITNNFILQGRARLNATLVPTDGSGVKAVFKTLKQGGFSIVL
HHHHHCCCCEEEEECCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEE
PDHVPDPSGGVIVPFFGIETLTSTLASKLASKTKCALVGLACIRRDDGHGFDIYCYKLDD
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
PALYDRNTETAAYALNLAMQRMIEDNYSHYMWGYRRFKHIPMIDNPYNTNKANLAAFIRA
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEE
HHPSVDNK
CCCCCCCC
>Mature Secondary Structure
MNPYHIFKIVPLSVLQMLARFVAWIILRMPSASIMRTVKINMALIAPTLSHLQKQALTKE
CCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHEEEHHHHHHHHHHHHHHHHHHHHH
IIYHQCLTSIESIKSWAMPPDWSIAQIRDVHNKDILLDGLASPNGMLAIVPHLGTWEMMN
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCHHHHH
AWLNQFGSPTIMYKPVKGDITNNFILQGRARLNATLVPTDGSGVKAVFKTLKQGGFSIVL
HHHHHCCCCEEEEECCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEE
PDHVPDPSGGVIVPFFGIETLTSTLASKLASKTKCALVGLACIRRDDGHGFDIYCYKLDD
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
PALYDRNTETAAYALNLAMQRMIEDNYSHYMWGYRRFKHIPMIDNPYNTNKANLAAFIRA
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEE
HHPSVDNK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]