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The map label for this gene is ilvE [H]

Identifier: 93006004

GI number: 93006004

Start: 1423215

End: 1424144

Strand: Direct

Name: ilvE [H]

Synonym: Pcryo_1176

Alternate gene names: 93006004

Gene position: 1423215-1424144 (Clockwise)

Preceding gene: 93006003

Following gene: 93006005

Centisome position: 46.51

GC content: 45.16

Gene sequence:

>930_bases
ATGAATATGGCAACAAAGGAAGGCAAGCTTTGGATGAATGGCACGATGATTGAACAGCCAGATGCCAAAATTCATGTGCT
TACGCACAGCATGCATTATGGTATCGCAGTCTTTGAAGGCGTCCGTGCCTATCAAACCGCTGATAACCGTACCGCGATTT
TCCGCTTAAAAGAACATACTGAGCGTCTGCTAGGTTCGGCAAAGATTTTCCAAATGGATGTGCCTTTTGATGCAGCAACA
CTTGAGCAAGCACACAAAGATGTGGTCAAGCAGAACAATCTAGCAGAAGCTTATATCCGCCCGCTGATTTGGTTCGGTGC
CGAAAAACTGGGTCTATCATCACGTGATAATAGCGTTAATGCCATGGTAGCTGCTTGGCATTGGGGCGCGTACCTTGGTG
AAGAAGGTATCAAAAACGGTATTCGTGTAAAAACGTCATCATATACCCATCATTTGCCAAACGTGACCATGTGTAAAGCA
AAAGCCTCAAGTAACTATCCTGTATCTATCATGGCAAACCAAGAAGTGACCCGTAACGGCTATGACGAAGCCATCTTGAT
GGATCCACAAGGCTATGTCTGCCAAGGCGCTGGCGAGAACTTATTTTTAGTTAAAGATGGTGTATTGCATACCCCTGATT
TAGCAGGCGGGGCACTTGACGGTATCACGCGTCGAACTATTGTCCAATTTGCTGAAGATTTGGGCATTAAAGTTGTTGAG
CGCCGTATCACTCGTGATGAGTTTTACTTAGCGGATGAAATATTTATGACAGGTACCGCTGCTGAAGTCACGCCTATTCG
TGAATACGATGATCGTACTATCGGTAATGGTGGTCGCGGACCCTTGACTGAAAAGCTGCAAACCTTGTACTTTGATGTGG
TCCATGGTCGCAATGAGCAGTATATGGATTGGTTAAGTTTTATTGACTGA

Upstream 100 bases:

>100_bases
TAAAACAGACACTCAGTGTCATTATAAAATTTTAATTAAAACATTTACTTAACACTCAACAAAAAAAGTTAGACGTTAGC
GCTAGCATAAGGATTATTAG

Downstream 100 bases:

>100_bases
CATCAAGTTTGTTTGAATTAATAAAAAGGGGCTGAATATACTCATTTAGTTCCTTTTTATATTTTCTATAAACTTAAATT
ATATTTAAATTAAAAATATC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNMATKEGKLWMNGTMIEQPDAKIHVLTHSMHYGIAVFEGVRAYQTADNRTAIFRLKEHTERLLGSAKIFQMDVPFDAAT
LEQAHKDVVKQNNLAEAYIRPLIWFGAEKLGLSSRDNSVNAMVAAWHWGAYLGEEGIKNGIRVKTSSYTHHLPNVTMCKA
KASSNYPVSIMANQEVTRNGYDEAILMDPQGYVCQGAGENLFLVKDGVLHTPDLAGGALDGITRRTIVQFAEDLGIKVVE
RRITRDEFYLADEIFMTGTAAEVTPIREYDDRTIGNGGRGPLTEKLQTLYFDVVHGRNEQYMDWLSFID

Sequences:

>Translated_309_residues
MNMATKEGKLWMNGTMIEQPDAKIHVLTHSMHYGIAVFEGVRAYQTADNRTAIFRLKEHTERLLGSAKIFQMDVPFDAAT
LEQAHKDVVKQNNLAEAYIRPLIWFGAEKLGLSSRDNSVNAMVAAWHWGAYLGEEGIKNGIRVKTSSYTHHLPNVTMCKA
KASSNYPVSIMANQEVTRNGYDEAILMDPQGYVCQGAGENLFLVKDGVLHTPDLAGGALDGITRRTIVQFAEDLGIKVVE
RRITRDEFYLADEIFMTGTAAEVTPIREYDDRTIGNGGRGPLTEKLQTLYFDVVHGRNEQYMDWLSFID
>Mature_309_residues
MNMATKEGKLWMNGTMIEQPDAKIHVLTHSMHYGIAVFEGVRAYQTADNRTAIFRLKEHTERLLGSAKIFQMDVPFDAAT
LEQAHKDVVKQNNLAEAYIRPLIWFGAEKLGLSSRDNSVNAMVAAWHWGAYLGEEGIKNGIRVKTSSYTHHLPNVTMCKA
KASSNYPVSIMANQEVTRNGYDEAILMDPQGYVCQGAGENLFLVKDGVLHTPDLAGGALDGITRRTIVQFAEDLGIKVVE
RRITRDEFYLADEIFMTGTAAEVTPIREYDDRTIGNGGRGPLTEKLQTLYFDVVHGRNEQYMDWLSFID

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=309, Percent_Identity=44.0129449838188, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17565728, Length=293, Percent_Identity=27.3037542662116, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322608, Length=263, Percent_Identity=26.615969581749, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322002, Length=308, Percent_Identity=25, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34652; Mature: 34652

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMATKEGKLWMNGTMIEQPDAKIHVLTHSMHYGIAVFEGVRAYQTADNRTAIFRLKEHT
CCCCCCCCEEEECCEEEECCCCEEEEEEECCHHCHHHHHCHHHHHCCCCCEEEEEEHHHH
ERLLGSAKIFQMDVPFDAATLEQAHKDVVKQNNLAEAYIRPLIWFGAEKLGLSSRDNSVN
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHCCCCCCCCCE
AMVAAWHWGAYLGEEGIKNGIRVKTSSYTHHLPNVTMCKAKASSNYPVSIMANQEVTRNG
EEEEEEHHHHHHCHHHHCCCEEEEECCCEECCCCEEEEEECCCCCCCEEEEECCCHHCCC
YDEAILMDPQGYVCQGAGENLFLVKDGVLHTPDLAGGALDGITRRTIVQFAEDLGIKVVE
CCCEEEECCCCCEEECCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHH
RRITRDEFYLADEIFMTGTAAEVTPIREYDDRTIGNGGRGPLTEKLQTLYFDVVHGRNEQ
HHHCCCHHEEEHHEEEECCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
YMDWLSFID
HHHHHHHCC
>Mature Secondary Structure
MNMATKEGKLWMNGTMIEQPDAKIHVLTHSMHYGIAVFEGVRAYQTADNRTAIFRLKEHT
CCCCCCCCEEEECCEEEECCCCEEEEEEECCHHCHHHHHCHHHHHCCCCCEEEEEEHHHH
ERLLGSAKIFQMDVPFDAATLEQAHKDVVKQNNLAEAYIRPLIWFGAEKLGLSSRDNSVN
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHCCCCCCCCCE
AMVAAWHWGAYLGEEGIKNGIRVKTSSYTHHLPNVTMCKAKASSNYPVSIMANQEVTRNG
EEEEEEHHHHHHCHHHHCCCEEEEECCCEECCCCEEEEEECCCCCCCEEEEECCCHHCCC
YDEAILMDPQGYVCQGAGENLFLVKDGVLHTPDLAGGALDGITRRTIVQFAEDLGIKVVE
CCCEEEECCCCCEEECCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHH
RRITRDEFYLADEIFMTGTAAEVTPIREYDDRTIGNGGRGPLTEKLQTLYFDVVHGRNEQ
HHHCCCHHEEEHHEEEECCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
YMDWLSFID
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]